#mesoSPIM
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mesospim.bsky.social
Dual #mesoSPIM by Francesco Giardini, Leonardo Sacconi and team, with two telecentric lenses and sCMOS cameras for imaging and reconstruction of entire mouse heart 🐭 💖 One heart in 25 minutes 🚂 for high-res 3D map of cardiomyocyte orientations and laminar organization. doi.org/10.1113/JP29...
High‐throughput, high‐fidelity reconstruction of whole mouse‐heart cytoarchitecture at cellular scale
Abstract figure legend Experimental workflow of the dual-camera light-sheet microscope (dual-mesoSPIM) pipeline. Intact adult mouse hearts undergo CUBIC-based delipidation and refractive-index matchi...
doi.org
October 20, 2026 at 3:02 PM
brandy-syglass.bsky.social
🔬 A #3DThursday fan favorite from #BINA2026! This is a tiny, lab-grown model of a lung, meant to mimic the structure and function of real tissues. I think we showed this #organoid to everyone who stopped by ...💜

🔬: #mesoSPIM Lightsheet
🥼: Hei Ming Lai, Illumos
🎬: #syGlass, v2.7.1

#I2KxBINA2026
October 8, 2026 at 3:59 PM
nvladimus.bsky.social
Did you see their custom dual-camera #mesoSPIM? :)
August 20, 2026 at 6:22 AM
nvladimus.bsky.social
Indeed! I managed to generate a decent online documentation for our #mesoSPIM project in 2h, something I was procrastinating for 5 years. Feels like magic.
March 4, 2026 at 8:41 AM
nvladimus.bsky.social
We are trying to materialize this idea with Benchtop #mesoSPIM. Make a robust framework and let people do crazy things with it.
February 6, 2025 at 6:38 PM
nvladimus.bsky.social
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
January 20, 2025 at 10:22 AM
nvladimus.bsky.social
A monumental work by Hei Ming LAI and team. Deep staining of centimeter-scale tissues, mouse or human, in 3 days! Tested with over 350 antibodies! Multiple rounds! Antibodies or RNA FISH! Uses 2 low-cost chemicals in addition to standard iDISCO protocol!

Imaged with what? With #mesoSPIM !
💪💯
brandy-syglass.bsky.social Brandy A Freitas @brandy-syglass.bsky.social · Jan 16
Happy #3DThursday! Step into a kidney, captured in stunning detail using INSIHGT. This isn’t just beautiful; it’s vital, transforming how we study health, aging, and disease. I'm lost in it, I love it.🎨🔬

Imaged by Hei Ming Lai, CUHK
Visualized in syGlass.io
Reference: www.nature.com/articles/s41...
January 16, 2025 at 4:36 PM
nvladimus.bsky.social
Did you know? Our Center for Microscopy and Image Analysis has detailed step-by-step instructions (zmb.dozuki.com/c/Lightsheet...) how to operate and troubleshoot your #mesoSPIM microscope. By
@mgarbelli.bsky.social and Jose Maria Mateos Melero, for users like you 😉
December 17, 2024 at 2:35 PM
nvladimus.bsky.social
The mesoSPIM project in 2024, The Year of Benchtop 🎆
mailchi.mp/dced0daca419...
(image courtesy Tchern Lenn) #mesoSPIM
December 12, 2024 at 4:02 PM
brandy-syglass.bsky.social
It was actually the first mesoSPIM dataset I ever rendered and I’ve been so happy to learn the history of it. Did you go to the anniversary conference this year? Michael made it but I was in Montreal at BINA.
October 23, 2025 at 6:34 PM
brandy-syglass.bsky.social
The #mesoSPIM community just celebrated 10 years of open-source light-sheet microscopy in Zürich! For #3DThursday, we're honoring Martina Schaettin & Fabian Voigt’s iconic 3D chick embryo dataset — a luminous benchmark for open science and shared discovery.
#Microscopy #Lightsheet #syGlass
October 23, 2025 at 3:33 PM
brandy-syglass.bsky.social
Organoids for #3DThursday! This is a tiny, lab-grown 3D model of a lung, meant to mimic the structure and function of real tissues.

🔬: #mesoSPIM #Lightsheet
🧫: Lung Organoid
🥼: Hei Ming Lai
🏛️: Illumos Ltd, www.nature.com/articles/s41...
🎬: syGlass.io

#SciComm #TissueEngineering #Organoid #syGlass
August 7, 2025 at 9:45 AM
brandy-syglass.bsky.social
For #3DThursday, whole mouse liver imaged with #mesoSPIM light sheet microscopy! Showing how structure meets function across the organ.
🥽: syGlass.io
🥼: Hei Ming Lai
📝: INSIHGT -> www.nature.com/articles/s41...
🏛️: Chinese University of Hong Kong

#LightSheet #SciComm #3DImaging #syGlass
May 15, 2025 at 4:31 PM
ierp-inrae.bsky.social
🚀 Nouvelle étape pour #MesoSPIM !
Après avoir monté le microscope à l'IERP de l'INRAE Jouy-en-Josas avec des physiciens de l'ENS Paris-Saclay 🤝, il devient transportable 🧳.

Rendez-vous à #MiFoBio & #JST_RmuI pour le découvrir en vrai 🔬✨

@inrae-france.bsky.social @gdrimabio.bsky.social
October 3, 2025 at 1:58 PM
ierp-inrae.bsky.social
🔬 On June 6, we inaugurated the first #MesoSPIM in France at INRAE Jouy-en-Josas & ENS Paris-Saclay! A big thanks to @nvladimus.bsky.social , @mgarbelli.bsky.social ,Angeliki Vavladeli & all speakers and sponsors. Exciting step forward for #opensource imaging & interdisciplinary science! #microscopy
June 10, 2025 at 10:34 AM
sdvupsaclay.bsky.social
RAPPEL ! Inauguration MesoSPIM à l'IERP - 6 juin 2025
RAPPEL ! Inauguration MesoSPIM à l'IERP - 6 juin 2025
En 2024, l’IERP (INRAE) et le LuMin (ENS Paris Saclay) ont lancé l’initiative MesoSPIM Paris Saclay pour le montage d’un système open source dédié à l’imagerie 3D à feuille de lumière d’échantillons de grande taille transparisés (ex organes murins ou poissons entiers de 4 cm de long). -> Voir notre récent Focus Plateforme   Ce système unique en France, est entièrement ouvert à la communauté et vise à rassembler des communautés (biologistes, physiciens, mathématiciens) pour promouvoir des avancées en imagerie des systèmes complexes et en traitement des données (AI, détection et identification de profils pathologiques, intégration de données multimodales, aide au diagnostic assisté par ordinateur, ..) pour des applications en Santé (humaine et animale).   Nous organisons une journée de lancement le 6 juin 2025 à INRAE, Jouy en Josas ouverte à tous (site web)   L’objectif est triple : Information et formation : comprendre le fonctionnement et les capacités du mesoSPIM avec des sessions pratiques. Fédération : rassembler une communauté d’utilisateurs/développeurs potentiels et renforcer les connexions interdisciplinaires Dissémination : améliorer la visibilité de l'Université Paris-Saclay au sein des communautés de microscopie nationale et internationale.    Le programme est disponible sur le site de l’évènement.   Accueil et Introduction : un MesoSPIM dans le périmètre de Paris-Saclay, Christelle Langevin - François Marquier Nikita Vladimorov (Keynote) (Zurich University) Benchtop mesoSPIM : a next-generation open-source light-sheet microscope for cleared samples  Alexandra Fragola (Institut des sciences moléculaires d’Orsay,  Université Paris-Saclay) Adaptive optics light-sheet microscopy for in vivo imaging Coffee break Maxence Fretaud (INRAE, Université Paris-Saclay) Title coming soon.  Maxence Wisztorski, Isabelle Fournier (Lille University) Toward High Spatially Resolved Proteomics Using Expansion Microscopy  Hugues Talbot (CentraleSupélec - IA Saclay). Title coming soon.  Lunch Poster Session / MesoSPIM demonstration in small groups     Les inscriptions sont gratuites mais obligatoires, le nombre de places étant limitées.
sco.lt
May 18, 2025 at 7:55 PM
sdvupsaclay.bsky.social
RAPPEL ! Inauguration MesoSPIM à l'IERP - 6 juin 2025 sco.lt/6fNT9c
RAPPEL ! Inauguration MesoSPIM à l'IERP - 6 juin 2025 | Life Sciences Université Paris-Saclay
En 2024, l’IERP (INRAE) et le LuMin (ENS Paris Saclay) ont lancé l’initiative MesoSPIM Paris Saclay pour le montage d’un système open source dédié à l’imagerie 3D à feuille de lumière d’échantillons d...
sco.lt
March 27, 2025 at 2:59 PM
sdvupsaclay.bsky.social
SAVE THE DATE ! Inauguration MesoSPIM à l'IERP - 6 juin 2025 sco.lt/5qudI8
SAVE THE DATE ! Inauguration MesoSPIM à l'IERP - 6 juin 2025 | Life Sciences Université Paris-Saclay
En 2024, l’IERP (INRAE) et le LuMin (ENS Paris Saclay) ont lancé l’initiative MesoSPIM Paris Saclay pour le montage d’un système open source dédié à l’imagerie 3D à feuille de lumière d’échantillons de grande taille transparisés (ex organes murins ou poissons entiers de 4 cm de long). -> Voir not...
sco.lt
March 11, 2025 at 11:10 PM
sdvupsaclay.bsky.social
FOCUS PLATEFORME : L'IERP et le LuMIn lancent l'initiative MesoSPIM Paris Saclay - une première en France sco.lt/6juBtI
FOCUS PLATEFORME : L'IERP et le LuMIn lancent l'initiative MesoSPIM Paris Saclay - une première en France | Life Sciences Université Paris-Saclay
L’unité INRAE / Infectiologie Expérimentale des Rongeurs et Poissons (IERP) et l'unité ENS Paris Saclay/ Lumière, Matière et Interfaces (LuMIn) ont lancé l'initiative MesoSPIM Paris-Saclay en septembr...
sco.lt
March 2, 2025 at 4:40 PM
mads100tist.bsky.social
I'm busing the mesoSPIM today and I'm jot gonna lie I'm gonna pee myself from the excitement @imcf-basel.bsky.social
A mesoSPIM microscope. Imagine a LEGO construction, but it's metal and black. Lots of vertical and horizontal metal stuff, silver bolts and cables. It's sitting on a table, next to a joystick.
February 2, 2026 at 1:12 PM
jsdaniel02.bsky.social
I'm excited to be able to be in Zurich for the mesoSPIM symposium. This is a really fantastic project.
May 15, 2025 at 6:24 PM
jcregg.bsky.social
Very happy to have contributed to this study! Congrats to all the authors - an amazing tour de force integrating tissue clearing, mesoSPIM-ultra imaging, and cm-scale single-neuron tracing.
voigtvision.bsky.social voigtvision.bsky.social @voigtvision.bsky.social · Jul 6
🚨 New Preprint! 🚨: A new method for quantifying #tissue #clearing and a next-get #lightsheet #Microscope - the #mesoSPIM ultra by Marko Pende, @jcregg.bsky.social, @murawalalab.bsky.social @mdibl.org & many more!
Preprint: www.biorxiv.org/content/10.6...
Github: github.com/mesoSPIM/mes...
July 8, 2026 at 1:08 PM
jasonmileskirk.bsky.social
New chamber mount design for our @mesospim.bsky.social - magnetic kinematic bases from @thorlabs.bsky.social for the win! 3D printed (for now) bases hold the chamber snugly for precise positioning between experiments. Fill port on the front allows for media addition without disturbing imaging.
Stainless Imaging chamber for mesoSPIM with 3D printed kinematic mounts
June 3, 2025 at 7:09 PM
natcardiovascres.nature.com
❤️Our November issue is out❤️Read about an imaging method that combines cardiac electrophysiology with 3D structural heart reconstruction, a targeted glycophagy ATG8 therapy reversing diabetic heart disease, and more.

www.nature.com/natcardiovas...
Image: Leonardo Sacconi, Institute of Clinical Physiology. Cover design: Bethany Vukomanovic Cardiac fibrosis and arrhythmia Three-dimensional structural reconstruction of entire cleared mouse ventricles from a diseased heart acquired using mesoSPIM tomography. Myocardium is visualized by fluorescence (gray), and fibrosis is detected via scattered light (red) (see Giardini et al.).
November 13, 2025 at 1:50 PM
ijpb-versaillescly.bsky.social
🔬 Afternoon workshops at #IJPB covered a wide range of cutting-edge imaging techniques: FLIM & STED, microfluidics, expansion microscopy, pH biosensors, laser microdissection, Mesospim, and more! #RMUI

@inrae-france.bsky.social
November 6, 2025 at 4:56 PM