Now online! CRISPR mutagenesis of the MYC locus reveals a druggable ultraconserved RNA element
CRISPR mutagenesis of the MYC locus reveals a druggable ultraconserved RNA element
CRISPR tiling mutagenesis of the 10-kb human MYC locus mapped the fitness landscape of an entire human gene at base-pair resolution. This unbiased interrogation revealed that 67% of functionally essential base pairs are noncoding. An ultraconserved RNA regulatory element was identified that controls MYC localization and can be therapeutically targeted to suppress cancer cell growth in patient-derived tumor organoids.
dlvr.it
November 4, 2026 at 3:59 PM
Now online! CRISPR mutagenesis of the MYC locus reveals a druggable ultraconserved RNA element
Excited to share that our MYC mutagenesis paper is now online at Cell @cp-cell.bsky.social! Free access link through Dec 24 authors.elsevier.com/a/1o316L7PXy.... Special thanks to our handling editor Dr. Jude Nicholson and the reviewers for their support and feedback throughout the process!
November 4, 2026 at 5:04 PM
Excited to share that our MYC mutagenesis paper is now online at Cell @cp-cell.bsky.social! Free access link through Dec 24 authors.elsevier.com/a/1o316L7PXy.... Special thanks to our handling editor Dr. Jude Nicholson and the reviewers for their support and feedback throughout the process!
Check out our paper on therapeutic vulnerabilities of the human fungal pathogen Cryptococcus neoformans. We describe a method we call CRISPR-insert for genome mutagenesis. This is the first genome-wide CRISPR/Cas9 method implemented and validated in any fungal pathogen (to our knowledge). 1/3
November 5, 2026 at 9:55 PM
Check out our paper on therapeutic vulnerabilities of the human fungal pathogen Cryptococcus neoformans. We describe a method we call CRISPR-insert for genome mutagenesis. This is the first genome-wide CRISPR/Cas9 method implemented and validated in any fungal pathogen (to our knowledge). 1/3
CRISPR mutagenesis of the MYC locus reveals a druggable ultraconserved RNA element - ScienceDirect
CRISPR mutagenesis of the MYC locus reveals a druggable ultraconserved RNA element
Noncoding sequences comprise most of the human genome; however, their functional significance remains largely elusive. Using CRISPR tiling mutagenesis…
www.sciencedirect.com
November 6, 2026 at 2:06 AM
CRISPR mutagenesis of the MYC locus reveals a druggable ultraconserved RNA element - ScienceDirect
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/... #jcampubs
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation
Using targeted mutagenesis, this study shows that form II CODH does not contribute to CO oxidation in the model carboxydovore Paraburkholderia terrae COX and reveals that canonical maturation pathway...
enviromicro-journals.onlinelibrary.wiley.com
November 3, 2026 at 4:06 PM
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/... #jcampubs
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation - Dawson - 2026 - Environmental Microbiology - Wiley Online Library
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation
Using targeted mutagenesis, this study shows that form II CODH does not contribute to CO oxidation in the model carboxydovore Paraburkholderia terrae COX and reveals that canonical maturation pathway...
enviromicro-journals.onlinelibrary.wiley.com
November 3, 2026 at 10:50 AM
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation - Dawson - 2026 - Environmental Microbiology - Wiley Online Library
This has pretty significant consequences to those of us who work with infectious organisms and perform gene synthesis (and possibly PCR mutagenesis).
Anyone know what a "sequence of concern" is?
www.whitehouse.gov
November 6, 2026 at 1:15 PM
This has pretty significant consequences to those of us who work with infectious organisms and perform gene synthesis (and possibly PCR mutagenesis).
The new issue of Yeast (Vol.43, N.6) is out 😀, with exciting research on stress adaptation and mating-type specific gene expression, as well as reviews on genome engineering using transposons, and mutagenesis during quiescence!!!
👇 Browse the full issue here:
onlinelibrary.wiley.com/doi/10.1002/...
👇 Browse the full issue here:
onlinelibrary.wiley.com/doi/10.1002/...
Issue Information
No abstract is available for this article.
onlinelibrary.wiley.com
November 5, 2026 at 12:07 PM
The new issue of Yeast (Vol.43, N.6) is out 😀, with exciting research on stress adaptation and mating-type specific gene expression, as well as reviews on genome engineering using transposons, and mutagenesis during quiescence!!!
👇 Browse the full issue here:
onlinelibrary.wiley.com/doi/10.1002/...
👇 Browse the full issue here:
onlinelibrary.wiley.com/doi/10.1002/...
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation.
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation.
Published in Environmental microbiology
doi.org
November 3, 2026 at 8:00 AM
Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation.
🧬New today!
📄Deep #CRISPR mutagenesis predicts cancer risk in Li-Fraumeni syndrome
📄Deep #CRISPR mutagenesis predicts cancer risk in Li-Fraumeni syndrome
Deep CRISPR mutagenesis predicts cancer risk in Li-Fraumeni syndrome
TP53 encodes for the transcription factor TP53, which controls the expression of genes
involved in cell cycle, apoptosis, senescence, stem cell differentiation, metabolism,
and DNA repair.1 ∼50% of al...
www.cell.com
November 2, 2026 at 5:40 PM
🧬New today!
📄Deep #CRISPR mutagenesis predicts cancer risk in Li-Fraumeni syndrome
📄Deep #CRISPR mutagenesis predicts cancer risk in Li-Fraumeni syndrome
Is there mutagenesis?
November 1, 2026 at 2:00 PM
Is there mutagenesis?
🎓 Congratulations to Noé Seija on successfully defending his PhD thesis entitled: “Epigenetic and transcriptional determinants of AID mutagenesis in B cells” 👏
He was supervised by Dr. Javier Di Noia, Director of the Molecular biology of the B cell Research Unit.
He was supervised by Dr. Javier Di Noia, Director of the Molecular biology of the B cell Research Unit.
November 3, 2026 at 5:27 PM
🎓 Congratulations to Noé Seija on successfully defending his PhD thesis entitled: “Epigenetic and transcriptional determinants of AID mutagenesis in B cells” 👏
He was supervised by Dr. Javier Di Noia, Director of the Molecular biology of the B cell Research Unit.
He was supervised by Dr. Javier Di Noia, Director of the Molecular biology of the B cell Research Unit.
RRID:AB_228302 from @thermofishersci.bsky.social was used by authors in their Cell Reports paper. Thanks for making your methods matter! #accelerateopenscience #RRID #BetterScience
Heterogeneous contributions of APOBEC3 deaminases to substitution and indel mutagenesis in lung adenocarcinoma
Read the full paper: Heterogeneous contributions of APOBEC3 deaminases to substitution and indel mutagenesis in lung adenocarcinoma
doi.org
November 4, 2026 at 8:00 AM
RRID:AB_228302 from @thermofishersci.bsky.social was used by authors in their Cell Reports paper. Thanks for making your methods matter! #accelerateopenscience #RRID #BetterScience
Struggling with Q5® site-directed mutagenesis PCR? Primer design, template quality, annealing temperature, and reaction conditions can all make or break your experiment. Here's a practical troubleshooting guide to help scientists identify common pitfalls and improve PCR success. 🧬
November 3, 2026 at 1:30 AM
Struggling with Q5® site-directed mutagenesis PCR? Primer design, template quality, annealing temperature, and reaction conditions can all make or break your experiment. Here's a practical troubleshooting guide to help scientists identify common pitfalls and improve PCR success. 🧬
Frequent prion-based switching of DNA repair proteins boosts mutagenesis in S. cerevisiae, enabling rapid adaptation in diverse environments. Evolution just got a twist! PMID:42263676, Cell 2026, @Cell https://www.cell.com/cell/fulltext/S0092-8674(26)00577-5 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
https://www.cell.com/cell/fulltext/S0092-8674(26)00577-5
No description available
www.cell.com
November 5, 2026 at 1:00 PM
DMF, DCM, NMP, HBTU. Solid phase peptide synthesis is no fucking joke, and if even a few parts per million of those organic solvents carry over to the DP, people are going to get acute toxicity and long term mutagenesis.
November 2, 2026 at 3:10 PM
DMF, DCM, NMP, HBTU. Solid phase peptide synthesis is no fucking joke, and if even a few parts per million of those organic solvents carry over to the DP, people are going to get acute toxicity and long term mutagenesis.
New CRISPR prime editing targets histone H3, allowing precise changes to Lys sites. PMID:42420522, Nat Genet 2026, @NatureGenet @OTSociety @NAR_Open https://doi.org/10.1038/s41588-026-02675-y #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing | Nature Genetics
Histone post-translational modifications are fundamental to genome regulation, yet dissecting the functions of individual histone marks in mammals remains challenging due to the presence of multiple histone gene copies. Here we develop a high-throughput clustered regularly interspaced short palindromic repeats (CRISPR) prime editing platform enabling precise, reversible and combinatorial mutagenesis of canonical and noncanonical histone H3 genes within their native genomic context. Using systematic lysine-to-arginine substitutions benchmarked against synonymous controls, we identify key residues, including H3K4, H3K9, H3K14, H3K18 and H3K79, whose mutation compromises fitness in mouse embryonic stem cells. We further show that H3K56, linked to genome stability in yeast and Drosophila, has a conserved role in mammalian cells. Through analysis of selected double mutants, we uncover functional crosstalk across residues, with combinations such as H3K27R + H3K36R impairing stem cell self-re
doi.org
November 4, 2026 at 1:00 AM
modalities, reducing latency for 1 MB track predictions. Supports concurrent multi-variant evaluation for in silico saturation mutagenesis ( score_ism_variants ) without HTTP gateway timeouts. Supports model fold selection ( ALL_FOLDS, FOLD_0, FOLD_1, FOLD_2, and FOLD_3 )
November 5, 2026 at 12:20 AM
modalities, reducing latency for 1 MB track predictions. Supports concurrent multi-variant evaluation for in silico saturation mutagenesis ( score_ism_variants ) without HTTP gateway timeouts. Supports model fold selection ( ALL_FOLDS, FOLD_0, FOLD_1, FOLD_2, and FOLD_3 )
Charlotte Auerbach, «madre de la mutagénesis química»
Charlotte Auerbach, «madre de la mutagénesis química» - Mujeres con ciencia
Charlotte Auerbach. Mutagénesis química. Material genético. Agente tóxico. Madre de la mutagénesis química. Gas mostaza. Biomedicina.
mujeresconciencia.com
October 28, 2026 at 2:31 PM
Charlotte Auerbach, «madre de la mutagénesis química»
Mutagenesis to cover up engineering evidence - it's not new, you also have to remember: these are the same idiots like Peter Thiel who believe in their Transhumanist Immortality Bullshit that won't work - because they themselves don't even have a functional mind to transfer.
November 1, 2026 at 4:25 PM
Mutagenesis to cover up engineering evidence - it's not new, you also have to remember: these are the same idiots like Peter Thiel who believe in their Transhumanist Immortality Bullshit that won't work - because they themselves don't even have a functional mind to transfer.
3/9 But generating these distributions is only the beginning. We also want to understand what specifies specific chromatin states.
Introducing PRISM: Per-Read In Silico Mutagenesis. It reveals which DNA bases contribute to individual predicted configurations of TF binding and nucleosomes.
Introducing PRISM: Per-Read In Silico Mutagenesis. It reveals which DNA bases contribute to individual predicted configurations of TF binding and nucleosomes.
November 5, 2026 at 6:18 PM
3/9 But generating these distributions is only the beginning. We also want to understand what specifies specific chromatin states.
Introducing PRISM: Per-Read In Silico Mutagenesis. It reveals which DNA bases contribute to individual predicted configurations of TF binding and nucleosomes.
Introducing PRISM: Per-Read In Silico Mutagenesis. It reveals which DNA bases contribute to individual predicted configurations of TF binding and nucleosomes.
Genomic Correlates of APOBEC-Associated Mutagenesis in Esophageal Squamous Cell Carcinoma https://www.biorxiv.org/content/10.64898/2026.10.27.762354v1
November 1, 2026 at 2:33 AM
Genomic Correlates of APOBEC-Associated Mutagenesis in Esophageal Squamous Cell Carcinoma https://www.biorxiv.org/content/10.64898/2026.10.27.762354v1