Nature research paper: Mapping and engineering RNA-driven architecture of the multiphase nucleolus
https://go.nature.com/44Tib11
https://go.nature.com/44Tib11
Mapping and engineering RNA-driven architecture of the multiphase nucleolus - Nature
Spatially segregated rRNA processing dictates nucleolar morphology and drives outward progression of pre-ribosomal RNA through nucleolar phases.
go.nature.com
July 8, 2025 at 8:41 PM
Nature research paper: Mapping and engineering RNA-driven architecture of the multiphase nucleolus
https://go.nature.com/44Tib11
https://go.nature.com/44Tib11
Radial pH differences across sub-nucleolar zones, the FC, DFC, GC, aligned with ribosome biogenesis. No membrane. No ion channels.
Still, a gradient. 12/n
Still, a gradient. 12/n
December 17, 2025 at 12:46 AM
Radial pH differences across sub-nucleolar zones, the FC, DFC, GC, aligned with ribosome biogenesis. No membrane. No ion channels.
Still, a gradient. 12/n
Still, a gradient. 12/n
We found that copies of the LINE1 transposon are key “architects” of the nucleolar genome in human embryonic stem cells. This work brings together two facets of Barbara McClintock’s pioneering research: nucleolar organizer regions and transposable elements.
www.genesdev.org/cgi/doi/10.1...
www.genesdev.org/cgi/doi/10.1...
LINE1 elements at distal junctions of rDNA repeats regulate nucleolar organization in human embryonic stem cells
A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields
www.genesdev.org
January 2, 2025 at 8:36 PM
We found that copies of the LINE1 transposon are key “architects” of the nucleolar genome in human embryonic stem cells. This work brings together two facets of Barbara McClintock’s pioneering research: nucleolar organizer regions and transposable elements.
www.genesdev.org/cgi/doi/10.1...
www.genesdev.org/cgi/doi/10.1...
Excited to share Benson’s and Maria’s work in collab w/Kostas Tzelepis: we found the nucleolar protein NPM1 is a marker of AML and can be targeted therapeutically
connecting ‘cell surface RNA biology’ to cancer biology
@natbiotech.nature.com
www.nature.com/articles/s41...
connecting ‘cell surface RNA biology’ to cancer biology
@natbiotech.nature.com
www.nature.com/articles/s41...
April 23, 2025 at 11:44 AM
Excited to share Benson’s and Maria’s work in collab w/Kostas Tzelepis: we found the nucleolar protein NPM1 is a marker of AML and can be targeted therapeutically
connecting ‘cell surface RNA biology’ to cancer biology
@natbiotech.nature.com
www.nature.com/articles/s41...
connecting ‘cell surface RNA biology’ to cancer biology
@natbiotech.nature.com
www.nature.com/articles/s41...
New method drop:
HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning.
www.biorxiv.org/content/10.1...
HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning.
www.biorxiv.org/content/10.1...
June 2, 2025 at 10:30 AM
New method drop:
HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning.
www.biorxiv.org/content/10.1...
HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning.
www.biorxiv.org/content/10.1...
I am pleased to share our latest manuscript, a collaboration with my @sickkidsto.bsky.socical colleague Dr. Ran Kafri. Led by PhD student Rachel Webster, who found that the CDK4/Rb/E2F pathway regulates metabolism to prevent premature aging in C. elegans. www.nature.com/articles/s41...
CDK-4 regulates nucleolar size and metabolism at the cost of late-life fitness in C. elegans - Heredity
Heredity - CDK-4 regulates nucleolar size and metabolism at the cost of late-life fitness in C. elegans
www.nature.com
May 18, 2025 at 12:40 PM
I am pleased to share our latest manuscript, a collaboration with my @sickkidsto.bsky.socical colleague Dr. Ran Kafri. Led by PhD student Rachel Webster, who found that the CDK4/Rb/E2F pathway regulates metabolism to prevent premature aging in C. elegans. www.nature.com/articles/s41...
Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity, but how? This study shows that the nucleolar RNA #exosome surveillance mechanism can be temporarily inhibited, reducing translation in #Celegans to adapt to starvation @plosbiology.org 🧪 plos.io/3Zs1B6c
May 23, 2025 at 9:54 AM
Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity, but how? This study shows that the nucleolar RNA #exosome surveillance mechanism can be temporarily inhibited, reducing translation in #Celegans to adapt to starvation @plosbiology.org 🧪 plos.io/3Zs1B6c
Check out this amazing work from @wbderry.bsky.social lab on nucleolar size regulation by CDK-4 published in @heredityjournal.bsky.social as part of the C. elegans Special Issue. www.nature.com/articles/s41...
CDK-4 regulates nucleolar size and metabolism at the cost of late-life fitness in C. elegans - Heredity
Heredity - CDK-4 regulates nucleolar size and metabolism at the cost of late-life fitness in C. elegans
www.nature.com
May 28, 2025 at 8:30 AM
Check out this amazing work from @wbderry.bsky.social lab on nucleolar size regulation by CDK-4 published in @heredityjournal.bsky.social as part of the C. elegans Special Issue. www.nature.com/articles/s41...
Combining sequencing and imaging, @brangwynnelab.bsky.social maps the spatiotemporal dynamics of rRNA processing, demonstrating how rRNA serves as both a scaffold and a substrate for the nucleolus—a multiphase, liquid-like structure. 🧪 🧬
Mapping and engineering RNA-driven architecture of the multiphase nucleolus - Nature
Spatially segregated rRNA processing dictates nucleolar morphology and drives outward progression of pre-ribosomal RNA through nucleolar phases.
www.nature.com
July 14, 2025 at 2:06 PM
Combining sequencing and imaging, @brangwynnelab.bsky.social maps the spatiotemporal dynamics of rRNA processing, demonstrating how rRNA serves as both a scaffold and a substrate for the nucleolus—a multiphase, liquid-like structure. 🧪 🧬
Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity, but how? This study shows that the nucleolar RNA #exosome surveillance mechanism can be temporarily inhibited, reducing translation in #Celegans to adapt to starvation @plosbiology.org 🧪 plos.io/3Zs1B6c
May 22, 2025 at 4:48 PM
Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity, but how? This study shows that the nucleolar RNA #exosome surveillance mechanism can be temporarily inhibited, reducing translation in #Celegans to adapt to starvation @plosbiology.org 🧪 plos.io/3Zs1B6c
Very excited about our recent preprint- @lindashuyingxu.bsky.social and collabs discover that neutrophils repurpose their nucleolus for storage of pre-made IFN-I to be rapidly released via a nucleolar stress response that results in nucleolar extrusion and secretion.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 29, 2026 at 10:06 PM
Very excited about our recent preprint- @lindashuyingxu.bsky.social and collabs discover that neutrophils repurpose their nucleolus for storage of pre-made IFN-I to be rapidly released via a nucleolar stress response that results in nucleolar extrusion and secretion.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Online Now: The nucleolar granular component mediates genome-nucleolus interactions and establishes their repressive chromatin states Online now:
The nucleolar granular component mediates genome-nucleolus interactions and establishes their repressive chromatin states
Gupta et al. showed that NPM1, a nucleolar granular component factor, mediates NAD association with the nucleolus. NPM1 binds NADs and recruits histone methyltransferase G9a to establish H3K9me2 repressive chromatin, revealing that the nucleolus functions not only as a scaffold for NADs but also actively contributes to their chromatin repression.
dlvr.it
May 23, 2025 at 10:57 PM
Online Now: The nucleolar granular component mediates genome-nucleolus interactions and establishes their repressive chromatin states Online now:
Excited to share our new bioRxiv preprint on nucleolar dynamics during oogenesis! We show that oocytes extensively remodel nucleolar architecture during development to support massive ribosome production. Thanks to our collaborators from the Gerton lab!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
www.biorxiv.org
May 21, 2026 at 8:05 PM
Excited to share our new bioRxiv preprint on nucleolar dynamics during oogenesis! We show that oocytes extensively remodel nucleolar architecture during development to support massive ribosome production. Thanks to our collaborators from the Gerton lab!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
🧬 Sticky situation in the nucleolus?
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
May 31, 2025 at 10:02 AM
🧬 Sticky situation in the nucleolus?
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
Many congratulations to my former PhD student, Priyanka Dogra on her brilliant, tour de force, postdoctoral work from St. Jude on ribosome biogenesis in nucleolar granular component sub-phases, just out in Molecular Cell.
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Granular component sub-phases direct ribosome biogenesis in the nucleolus
Dogra et al. use super-resolution microscopy to uncover the molecular origins of spatial
heterogeneity in the nucleolar granular component (GC). They show that differential
interactions of nucleolar p...
www.cell.com
July 15, 2026 at 5:18 PM
Many congratulations to my former PhD student, Priyanka Dogra on her brilliant, tour de force, postdoctoral work from St. Jude on ribosome biogenesis in nucleolar granular component sub-phases, just out in Molecular Cell.
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
IDPSeminars is back this week (Tomorrow, March 6th)! Come hear about nucleolar organization and IDRs in host-pathogen interactions!
PST 10 am
EST 1 pm
CET 7 pm!
Info: idpseminars.com
Signup form: forms.gle/3yCHeYvuuqLh...
PST 10 am
EST 1 pm
CET 7 pm!
Info: idpseminars.com
Signup form: forms.gle/3yCHeYvuuqLh...
March 5, 2025 at 1:14 PM
IDPSeminars is back this week (Tomorrow, March 6th)! Come hear about nucleolar organization and IDRs in host-pathogen interactions!
PST 10 am
EST 1 pm
CET 7 pm!
Info: idpseminars.com
Signup form: forms.gle/3yCHeYvuuqLh...
PST 10 am
EST 1 pm
CET 7 pm!
Info: idpseminars.com
Signup form: forms.gle/3yCHeYvuuqLh...
New preprint 📜 from @AlanMonziani in the lab – EPB41L4A-AS1 long noncoding RNA acts in both cis- and trans-acting transcriptional regulation and controls nucleolar biology www.biorxiv.org/content/10.1... . A🧵
EPB41L4A-AS1 long noncoding RNA acts in both cis- and trans-acting transcriptional regulation and controls nucleolar biology
Mammalian genomes are pervasively transcribed into long noncoding RNAs (lncRNAs), whose functions and modes of action remain poorly understood. EPB41L4A-AS1 is an evolutionary conserved, broadly and h...
www.biorxiv.org
April 10, 2025 at 5:58 AM
New preprint 📜 from @AlanMonziani in the lab – EPB41L4A-AS1 long noncoding RNA acts in both cis- and trans-acting transcriptional regulation and controls nucleolar biology www.biorxiv.org/content/10.1... . A🧵
Jessica Sheu-Gruttadauria et al. show that the material properties of the #nucleolus are shaped by the assembly state of ribosomal precursors, which tune nucleolar dynamics and morphology through their interactions with the nucleolar scaffolding protein NPM1. rupress.org/jcb/article/...
#Biophysics
#Biophysics
September 9, 2026 at 4:45 PM
Jessica Sheu-Gruttadauria et al. show that the material properties of the #nucleolus are shaped by the assembly state of ribosomal precursors, which tune nucleolar dynamics and morphology through their interactions with the nucleolar scaffolding protein NPM1. rupress.org/jcb/article/...
#Biophysics
#Biophysics
Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity, but how? This study shows that the nucleolar RNA #exosome surveillance mechanism can be temporarily inhibited, reducing translation in #Celegans to adapt to starvation @plosbiology.org 🧪 plos.io/3Zs1B6c
May 22, 2025 at 1:30 PM
Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity, but how? This study shows that the nucleolar RNA #exosome surveillance mechanism can be temporarily inhibited, reducing translation in #Celegans to adapt to starvation @plosbiology.org 🧪 plos.io/3Zs1B6c
🥳 🎉 New preprint from the lab is out!
It's time to officially introduce the world to the Nucleolar Integrity and Stress Microprotein (NISM)!
Check out the preprint in the link below
It's time to officially introduce the world to the Nucleolar Integrity and Stress Microprotein (NISM)!
Check out the preprint in the link below
HDAC5-encoded Microprotein NISM Mediates Nucleolar Formation and Ribosomal RNA Synthesis https://www.biorxiv.org/content/10.64898/2026.02.21.707204v1
February 23, 2026 at 3:49 PM
🥳 🎉 New preprint from the lab is out!
It's time to officially introduce the world to the Nucleolar Integrity and Stress Microprotein (NISM)!
Check out the preprint in the link below
It's time to officially introduce the world to the Nucleolar Integrity and Stress Microprotein (NISM)!
Check out the preprint in the link below
🧬 Sticky situation in the nucleolus?
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
June 13, 2025 at 8:21 AM
🧬 Sticky situation in the nucleolus?
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
A new study shows that the ‘Treacle’ protein forms liquid-like condensates that organise nucleolar structure and keep ribosome production flowing. #Genetics #CellBio
buff.ly/eyOFzvR
📰Happy to share our preprint.🥳Here we investigate🔬, the essential role of the conserved RNA helicase Drs1. We show that its engagement and activity license timely incorporation of 25S rRNA domain III into maturing nucleolar pre-60S subunits.👇
doi.org/10.64898/2026.09.05.749353
#cryoEM #ribosome #RNA
doi.org/10.64898/2026.09.05.749353
#cryoEM #ribosome #RNA
September 10, 2026 at 8:17 AM
📰Happy to share our preprint.🥳Here we investigate🔬, the essential role of the conserved RNA helicase Drs1. We show that its engagement and activity license timely incorporation of 25S rRNA domain III into maturing nucleolar pre-60S subunits.👇
doi.org/10.64898/2026.09.05.749353
#cryoEM #ribosome #RNA
doi.org/10.64898/2026.09.05.749353
#cryoEM #ribosome #RNA
