The human pangenome continues to grow and improve! Release 2 is here! Click through for the details, but this is a pretty amazing dataset including not just the phased assemblies, but PacBio HiFi, ONT Ultralong, Dovetail/Illumina Hi-C, PacBio Kinnex, and Illumina WGS for all samples
📢 HPRC Release 2 is here!
Now with phased genomes from 200+ individuals, a 5x increase from Release 1.
Explore sequencing data, assemblies, annotations & alignments in our interactive data explorer ⬇️:
humanpangenome.org/hprc-data-re...
Now with phased genomes from 200+ individuals, a 5x increase from Release 1.
Explore sequencing data, assemblies, annotations & alignments in our interactive data explorer ⬇️:
humanpangenome.org/hprc-data-re...
May 12, 2025 at 1:52 PM
The human pangenome continues to grow and improve! Release 2 is here! Click through for the details, but this is a pretty amazing dataset including not just the phased assemblies, but PacBio HiFi, ONT Ultralong, Dovetail/Illumina Hi-C, PacBio Kinnex, and Illumina WGS for all samples
Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social).
myloasm-docs.github.io
myloasm-docs.github.io
myloasm - metagenomic assembly with (noisy) long reads
myloasm-docs.github.io
May 28, 2025 at 5:54 PM
Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social).
myloasm-docs.github.io
myloasm-docs.github.io
Celebrating @udelaware.bsky.social Sequencing Center for their #HiFiSequencing record! 🤯 They generated 194Gb in a single Revio SPRQ run, powering crucial research on biological pest control. #PacBio #Genomics
March 19, 2025 at 8:06 PM
Celebrating @udelaware.bsky.social Sequencing Center for their #HiFiSequencing record! 🤯 They generated 194Gb in a single Revio SPRQ run, powering crucial research on biological pest control. #PacBio #Genomics
NGS and Multi-Omics November 2024 update. News from Illumina, Complete Genomics, PacBio, Element Bio and Singular Genomics open.substack.com/pub/albertvi...
NGS and Multi-Omics November 2024 update
News from Illumina, Complete Genomics, PacBio, Element Bio and Singular Genomics
open.substack.com
November 15, 2024 at 8:56 PM
NGS and Multi-Omics November 2024 update. News from Illumina, Complete Genomics, PacBio, Element Bio and Singular Genomics open.substack.com/pub/albertvi...
There's an opening in our CompBio group at #PacBio. Apply below if you're interested in method / tool development for long-read sequencing applications! #Bioinformatics
pacbio.wd12.myworkdayjobs.com/PacBio-/job/...
pacbio.wd12.myworkdayjobs.com/PacBio-/job/...
December 12, 2025 at 6:40 PM
There's an opening in our CompBio group at #PacBio. Apply below if you're interested in method / tool development for long-read sequencing applications! #Bioinformatics
pacbio.wd12.myworkdayjobs.com/PacBio-/job/...
pacbio.wd12.myworkdayjobs.com/PacBio-/job/...
New metagenome assembler for ONT R10 and PacBio HiFi reads, from Jim Shaw. See Jim's thread for details
Announcing myloasm, a new long-read (ONT R10/PacBio) metagenome assembler that I've been working on during my postdoc in the Heng Li lab (@lh3lh3.bsky.social).
myloasm-docs.github.io
myloasm-docs.github.io
myloasm - metagenomic assembly with (noisy) long reads
myloasm-docs.github.io
May 28, 2025 at 7:07 PM
New metagenome assembler for ONT R10 and PacBio HiFi reads, from Jim Shaw. See Jim's thread for details
We just got our 1st full-length 16S rRNA PacBio dataset back and it's a game changer (in the context of bacterial isolates)!
Do folks have recommendations for archaeal or bac-arch primer sets for PacBio 16S runs?
Do folks have recommendations for archaeal or bac-arch primer sets for PacBio 16S runs?
June 27, 2025 at 2:58 PM
We just got our 1st full-length 16S rRNA PacBio dataset back and it's a game changer (in the context of bacterial isolates)!
Do folks have recommendations for archaeal or bac-arch primer sets for PacBio 16S runs?
Do folks have recommendations for archaeal or bac-arch primer sets for PacBio 16S runs?
This PacBio lego puzzle was fun! But lorrrrrrdddyyyyy the instructions 🙈🙈🙈
June 24, 2025 at 11:57 PM
This PacBio lego puzzle was fun! But lorrrrrrdddyyyyy the instructions 🙈🙈🙈
Not new (from 2024) but very cool paper
www.nature.com/articles/s41...
They generated genomes of fruit flies and their Wolbachia endosymbionts from single individuals using PacBio sequencing.
www.nature.com/articles/s41...
They generated genomes of fruit flies and their Wolbachia endosymbionts from single individuals using PacBio sequencing.
Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes - Nature Communications
Pacific Biosciences (PacBio) High Fidelity (HiFi) long-read sequencing usually requires a relatively high amount of DNA input ( > 1 µg). Here the authors develop LILAP, a Low-Input (100 ng), Low-co...
www.nature.com
February 24, 2026 at 4:33 AM
Not new (from 2024) but very cool paper
www.nature.com/articles/s41...
They generated genomes of fruit flies and their Wolbachia endosymbionts from single individuals using PacBio sequencing.
www.nature.com/articles/s41...
They generated genomes of fruit flies and their Wolbachia endosymbionts from single individuals using PacBio sequencing.
“StarPhase: Comprehensive Phase-Aware Pharmacogenomic Diplotyper for Long-Read Sequencing Data” is now on biorxiv! In this work, we explore the use of long-read sequencing (#PacBio #HiFi) for #pharmacogenomics #PGx. 1/N
Pre-print: doi.org/10.1101/2024...
Repo: github.com/PacificBiosc...
Pre-print: doi.org/10.1101/2024...
Repo: github.com/PacificBiosc...
GitHub - PacificBiosciences/pb-StarPhase: A phase-aware pharmacogenomic diplotyper for PacBio datasets
A phase-aware pharmacogenomic diplotyper for PacBio datasets - PacificBiosciences/pb-StarPhase
github.com
December 11, 2024 at 2:30 PM
“StarPhase: Comprehensive Phase-Aware Pharmacogenomic Diplotyper for Long-Read Sequencing Data” is now on biorxiv! In this work, we explore the use of long-read sequencing (#PacBio #HiFi) for #pharmacogenomics #PGx. 1/N
Pre-print: doi.org/10.1101/2024...
Repo: github.com/PacificBiosc...
Pre-print: doi.org/10.1101/2024...
Repo: github.com/PacificBiosc...
Very pleased to share that we just released a new ref genome for Colias eurytheme and added the first genome for C philodice to NCBI. Both chrom-level, made with pacbio hi-fi and ci-fi (=pac bio chromatin reads). Both from MD. Please use and share widely, I’ll have a citable source preprinted soon.
September 27, 2026 at 6:15 PM
Very pleased to share that we just released a new ref genome for Colias eurytheme and added the first genome for C philodice to NCBI. Both chrom-level, made with pacbio hi-fi and ci-fi (=pac bio chromatin reads). Both from MD. Please use and share widely, I’ll have a citable source preprinted soon.
Need long-read sequencing? We just completed the first ever run on @uoregon.bsky.social GC3F core’s new
@pacbio.bsky.social Revio instrument! Perfect for amplicons too long for Illumina, and with Kinnex, you can get >10x more data. Great pricing, no queue, try it: gc3f.uoregon.edu/pacbio-seque...
@pacbio.bsky.social Revio instrument! Perfect for amplicons too long for Illumina, and with Kinnex, you can get >10x more data. Great pricing, no queue, try it: gc3f.uoregon.edu/pacbio-seque...
November 25, 2024 at 2:53 PM
Need long-read sequencing? We just completed the first ever run on @uoregon.bsky.social GC3F core’s new
@pacbio.bsky.social Revio instrument! Perfect for amplicons too long for Illumina, and with Kinnex, you can get >10x more data. Great pricing, no queue, try it: gc3f.uoregon.edu/pacbio-seque...
@pacbio.bsky.social Revio instrument! Perfect for amplicons too long for Illumina, and with Kinnex, you can get >10x more data. Great pricing, no queue, try it: gc3f.uoregon.edu/pacbio-seque...
Now live! Submit a 100 word proposal to win free #PacBio HiFi sequencing for #metagenomics. Long-read metagenomics is rapidly gaining traction and this is a great time to apply it to your research.
programs.pacb.com/l/1652/2026-...
programs.pacb.com/l/1652/2026-...
2026 PacBio SMRT Grant | Metagenomics
programs.pacb.com
May 28, 2026 at 3:20 PM
Now live! Submit a 100 word proposal to win free #PacBio HiFi sequencing for #metagenomics. Long-read metagenomics is rapidly gaining traction and this is a great time to apply it to your research.
programs.pacb.com/l/1652/2026-...
programs.pacb.com/l/1652/2026-...
From expanding tandem repeat databases to enabling multiomic profiling in a single run, #PacBio #HiFisequencing keeps pushing genomics forward. This month’s Powered by PacBio blog features five studies redefining clinical, microbial, and plant research.
Details here: bit.ly/3Cv33N8
Details here: bit.ly/3Cv33N8
Powered by PacBio: Selected publications from January 2025 - PacBio
January 2025 edition of the Powered by PacBio blog series explores how our technology is making impactful genomic discoveries.
bit.ly
February 6, 2025 at 8:16 PM
From expanding tandem repeat databases to enabling multiomic profiling in a single run, #PacBio #HiFisequencing keeps pushing genomics forward. This month’s Powered by PacBio blog features five studies redefining clinical, microbial, and plant research.
Details here: bit.ly/3Cv33N8
Details here: bit.ly/3Cv33N8
My colleague Dr Shannon Corrigan will be speaking at the PacBio workshop at #PAG32 (13.1., 12:50 to 3pm) on where we're at with our HiFi fish genomes and what we're doing with them!
www.linkedin.com/posts/pacbio...
www.linkedin.com/posts/pacbio...
PacBio on LinkedIn: #pacbio #pag32
At PAG 2025, we’ll be showcasing how PacBio HiFi sequencing is helping to advance research in plant and animal sciences. From conserving endangered species to…
www.linkedin.com
January 12, 2025 at 3:24 AM
My colleague Dr Shannon Corrigan will be speaking at the PacBio workshop at #PAG32 (13.1., 12:50 to 3pm) on where we're at with our HiFi fish genomes and what we're doing with them!
www.linkedin.com/posts/pacbio...
www.linkedin.com/posts/pacbio...
Long-read sequencing can revolutionise the way we monitor #biodiversity.
A comprehensive guide for using #ONT & #PacBio for analysis of biodiversity through #eDNA, #metabarcoding, metagenomics
Just published in @methodsinecoevol.bsky.social
besjournals.onlinelibrary.wiley.com/doi/10.1111/...
A comprehensive guide for using #ONT & #PacBio for analysis of biodiversity through #eDNA, #metabarcoding, metagenomics
Just published in @methodsinecoevol.bsky.social
besjournals.onlinelibrary.wiley.com/doi/10.1111/...
February 3, 2026 at 3:54 PM
Long-read sequencing can revolutionise the way we monitor #biodiversity.
A comprehensive guide for using #ONT & #PacBio for analysis of biodiversity through #eDNA, #metabarcoding, metagenomics
Just published in @methodsinecoevol.bsky.social
besjournals.onlinelibrary.wiley.com/doi/10.1111/...
A comprehensive guide for using #ONT & #PacBio for analysis of biodiversity through #eDNA, #metabarcoding, metagenomics
Just published in @methodsinecoevol.bsky.social
besjournals.onlinelibrary.wiley.com/doi/10.1111/...
We updated our SMRT Cell Sequencing 101 post to reflect how PacBio HiFi sequencing actually works today.
From SPRQ-Nx chemistry to what you can do in a single run, it’s a more current look at sequencing performance.
Read the full blog here: bit.ly/4uhjhi8
#PacBio #HiFisequencing #Seq101
From SPRQ-Nx chemistry to what you can do in a single run, it’s a more current look at sequencing performance.
Read the full blog here: bit.ly/4uhjhi8
#PacBio #HiFisequencing #Seq101
Sequencing 101: The SMRT Cell in PacBio long-read sequencing - PacBio
SMRT Cells are integral reaction centers for PacBio HiFi long-read sequencing. This article details what SMRT Cells are and what they can do.
https://bit.ly/4uhjhi8"
April 27, 2026 at 10:31 PM
We updated our SMRT Cell Sequencing 101 post to reflect how PacBio HiFi sequencing actually works today.
From SPRQ-Nx chemistry to what you can do in a single run, it’s a more current look at sequencing performance.
Read the full blog here: bit.ly/4uhjhi8
#PacBio #HiFisequencing #Seq101
From SPRQ-Nx chemistry to what you can do in a single run, it’s a more current look at sequencing performance.
Read the full blog here: bit.ly/4uhjhi8
#PacBio #HiFisequencing #Seq101
Congratulations @wshuai.bsky.social and co-authors!
We’re excited to introduce MODIFI, our new scalable method for detecting DNA modifications in PacBio metagenomic data and estimating ECE-host linkage.
Check out the preprint: www.biorxiv.org/content/10.6...
We’re excited to introduce MODIFI, our new scalable method for detecting DNA modifications in PacBio metagenomic data and estimating ECE-host linkage.
Check out the preprint: www.biorxiv.org/content/10.6...
www.biorxiv.org
March 30, 2026 at 10:12 PM
Congratulations @wshuai.bsky.social and co-authors!
We’re excited to introduce MODIFI, our new scalable method for detecting DNA modifications in PacBio metagenomic data and estimating ECE-host linkage.
Check out the preprint: www.biorxiv.org/content/10.6...
We’re excited to introduce MODIFI, our new scalable method for detecting DNA modifications in PacBio metagenomic data and estimating ECE-host linkage.
Check out the preprint: www.biorxiv.org/content/10.6...
#Epigenetics goes beyond DNA sequences, shaping how genes are expressed. With #PacBio #HiFisequencing, researchers access a truly comprehensive epigenome solution—analyzing DNA, RNA, and methylation patterns on a single platform.
Full blog here: bit.ly/4gfQbrW
Full blog here: bit.ly/4gfQbrW
Sequencing 101: Epigenetics - PacBio
Epigenetics can impact everything from development to disease. See how PacBio HiFi sequencing technology is changing the game.
bit.ly
February 4, 2025 at 4:46 PM
#Epigenetics goes beyond DNA sequences, shaping how genes are expressed. With #PacBio #HiFisequencing, researchers access a truly comprehensive epigenome solution—analyzing DNA, RNA, and methylation patterns on a single platform.
Full blog here: bit.ly/4gfQbrW
Full blog here: bit.ly/4gfQbrW
Do you sequence genomes? Then this is a must-read: www.biorxiv.org/content/10.1...
Strong sequencing bias in Nanopore and PacBio prevents assembly of Drosophila melanogaster Y-linked genes
Nanopore and PacBio are generally considered free from sequence composition bias, a key factor – alongside read length – that explains their success in producing high quality genome assemblies. Howeve...
www.biorxiv.org
March 5, 2025 at 12:55 PM
Do you sequence genomes? Then this is a must-read: www.biorxiv.org/content/10.1...
Loll I must have targeted commercials on my Samsung TV because I just saw a PacBio ad on one of the TV program commercial breaks.
December 21, 2024 at 12:15 AM
Loll I must have targeted commercials on my Samsung TV because I just saw a PacBio ad on one of the TV program commercial breaks.
SingleM v0.20.2 - ONT/PacBio input reads now supported (if somewhat inefficiently), plus improved Lyrebird database for phage profiling.
Microbial fraction is now prokaryotic fraction, easier to specify input genomes.
Microbial fraction is now prokaryotic fraction, easier to specify input genomes.
November 17, 2025 at 4:49 AM
SingleM v0.20.2 - ONT/PacBio input reads now supported (if somewhat inefficiently), plus improved Lyrebird database for phage profiling.
Microbial fraction is now prokaryotic fraction, easier to specify input genomes.
Microbial fraction is now prokaryotic fraction, easier to specify input genomes.