#supernatants
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dicleverly.bsky.social
That's how I ended up with vulture supernatants in my publication instead of culture supernatants.
🤦‍♀️
September 25, 2026 at 4:36 PM
ralfwittenbrink.bsky.social
Antihistaminika, insbesondere Histaminrezeptor-H1 (HRH1)-Antagonisten, hemmen wirksam die #SARSCoV2-Infektion

In dieser Studie wurde „nachgewiesen, dass HRH1 als alternativer Rezeptor für #SARSCoV2 fungiert, indem er direkt an das virale Spike-Protein bindet.🧵

#COVID19 #Corona
Fig 7 Antihistamine drugs prevented SARS-CoV-2 infection in transgenic hACE2 mice. (A) Acrivastine at serially diluted concentrations was premixed with authentic SARS-CoV-2 D614G viruses, followed by incubation with HEK293T-hACE2 cells. After 48 h, the viral RNA in the supernatants was extracted and quantified via RT-PCR-based detection. The IC50 value was calculated based on the relative number of viral RNA copies within each concentration. (B) The IC50 of triprolidine against the authentic SARS-CoV-2 D614G strain was measured and calculated as described in panel A. (C) Schematic of the animal infection assay. Four transgenic hACE2 mice (C57BL/6) were intravenously administrated with acrivastine at a dosage of 10 mg/kg of body weight. Mice in the control group were administrated with an equal volume of saline. Six hours later, all mice were intranasally challenged with 1 × 105 focus-forming units of the SARS-CoV-2 D614G virus. …
July 6, 2024 at 1:11 PM
kgandersen.bsky.social
Because that is exactly what the samples the scientists were carrying appear to have been - diagnostic specimens that were inactivated and hence harmless.

This is a well-defined and widely used term, yet this is never mentioned once in the complaint.

E.g.,: www.cbp.gov/border-secur...
Examples of Biological Materials Biological materials can include the following: • Cultured cells or tissue: such as nonrecombinant or recombinant products, hybridomas, or supernatants • Diagnostic specimens: such as blood, tissue, fluids, or other bodily material transported for the purposes of diagnosis or research
June 4, 2026 at 7:07 PM
adlunglab.com
🏁 Dive into the details of this groundbreaking work in Scientific Reports: 🔗 doi.org/10.1038/s415... 🧪 @natureportfolio.bsky.social
4/4
Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model
Scientific Reports - Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model
doi.org
January 8, 2025 at 7:00 AM
viroscope.bsky.social
Atomic Force Microscopy, within the BSL-3 environment @cemipai.bsky.social with AFM wizard Sébastien Lyonnais, allowed us to probe native infectious particle topology at the single virion level, revealing that these hybrid viral particles exist at the native state in viral supernatants.
May 10, 2025 at 8:12 AM
sjorsscheres.bsky.social
I haven't yet found convincing evidence that such oligomers are present in human brains. Previously, @andrewmstern.bsky.social showed that so-called soluble oligomeric fractions of AD brains contained #Abeta filaments. When spun down, toxicity disappeared!

www.sciencedirect.com/science/arti...
Abundant Aβ fibrils in ultracentrifugal supernatants of aqueous extracts from Alzheimer’s disease brains
Soluble oligomers of amyloid β-protein (Aβ) have been defined as aggregates in supernatants following ultracentrifugation of aqueous extracts from Alz…
www.sciencedirect.com
March 14, 2025 at 4:25 PM
esiode.bsky.social
📃Scientific paper: Liquid biopsy using ascitic fluid and pleural effusion supernatants for genomic profiling in gastroi...

➡️ Continued on ES/IODE

April 6, 2024 at 2:41 AM
biorxiv-microbiol.bsky.social
A simple procedure to demonstrate antimicrobial activity in cell-free supernatants https://www.biorxiv.org/content/10.64898/2026.06.22.733903v1
June 24, 2026 at 3:18 AM
plosbiology.org
Archaea-on-bacteria action! @romainstrock.bsky.social @tobiaswarnecke.bsky.social &co show that many #archaea encode #peptidoglycan hydrolases, which specifically target #bacterial cell walls, experimentally confirming the killing capacity of 2 of these enzymes @plosbiology.org 🧪 plos.io/4lrJBBa
Left: Results of spotting various supernatants onto lawns of Halalkalibacterium halodurans, Phycicoccus endophyticus, and Virgibacillus salexigens. a,b: top fraction (>3 kDa) of supernatant from Haloferax volcanii expressing an intact (a) or catalytic mutant (b) version of Woldo; c,d: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing an intact (c) or catalytic mutant (d) version of Woldo; e: unfiltered supernatant from Halogranum salarium B-1; f,g: top fraction (>3 kDa) of supernatant from H. volcanii expressing (f) Danwoldo or (g) and empty plasmid control; h,i: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing (h) Danwoldo or (i) and empty plasmid control. Top right: Structural overlay of zoocin A from Streptococcus zooepidemicus (UniProt ID: O54308) and Woldo, a candidate PGH from Halogranum salarium B-1 (UniProt ID: J2ZZK6), highlighting homologous M23 domains but divergent cell wall binding domains. TRD: target recognition domain. PG: peptidoglycan. Bottom right: Images of bacterial cells following exposure to H. volcanii supernatants expressing Woldo, Danwoldo or the control (as above). Bacteria are stained using LIVE/DEAD BacLight bacterial viability staining kit with ingress of red dye into bacterial cells indicative of cells with terminally compromised cell wall integrity.
August 15, 2025 at 4:29 PM
42inabook.bsky.social
The reality for Supernatants is that “ time accumulation of contribution returns” is essential to a liveable balance (pension) on retirement

The genesis of the whole deal was a progressive increase from 9 to 15 % over time ….Not the reduction of contributions at any point

Workers sold out again
September 13, 2026 at 12:31 AM
pallettlab.bsky.social
Anyone got any advice/assays that work for measuring TGFb in cell culture supernatants 🧪 #immunosky

Experiment failure today!!!
December 19, 2024 at 4:58 PM
eternalcatwar.bsky.social
also lmao the microplates in the stock photo here suck for most applications unless you’re trying to pull off supernatants
February 11, 2025 at 6:45 AM
plosbiology.org
Archaea-on-bacteria action! @romainstrock.bsky.social @tobiaswarnecke.bsky.social &co show that many #archaea encode #peptidoglycan hydrolases, which specifically target #bacterial cell walls, experimentally confirming the killing capacity of 2 of these enzymes @plosbiology.org 🧪 plos.io/4lrJBBa
Left: Results of spotting various supernatants onto lawns of Halalkalibacterium halodurans, Phycicoccus endophyticus, and Virgibacillus salexigens. a,b: top fraction (>3 kDa) of supernatant from Haloferax volcanii expressing an intact (a) or catalytic mutant (b) version of Woldo; c,d: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing an intact (c) or catalytic mutant (d) version of Woldo; e: unfiltered supernatant from Halogranum salarium B-1; f,g: top fraction (>3 kDa) of supernatant from H. volcanii expressing (f) Danwoldo or (g) and empty plasmid control; h,i: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing (h) Danwoldo or (i) and empty plasmid control. Top right: Structural overlay of zoocin A from Streptococcus zooepidemicus (UniProt ID: O54308) and Woldo, a candidate PGH from Halogranum salarium B-1 (UniProt ID: J2ZZK6), highlighting homologous M23 domains but divergent cell wall binding domains. TRD: target recognition domain. PG: peptidoglycan. Bottom right: Images of bacterial cells following exposure to H. volcanii supernatants expressing Woldo, Danwoldo or the control (as above). Bacteria are stained using LIVE/DEAD BacLight bacterial viability staining kit with ingress of red dye into bacterial cells indicative of cells with terminally compromised cell wall integrity.
August 15, 2025 at 8:13 AM
asm.org
Natural and commercially available lung surfactants minimize the cytolytic capacity of S. aureus supernatants against primary human cells, in part by reducing transcript abundance of virulence factors. Learn more in #JournalSpectrum: asm.social/2oC
Surfactant from mouse and rat lungs protects human primary cells from S. aureus-mediated cytotoxicity.
May 2, 2025 at 5:52 PM
christinafaher1.bsky.social
Is the rumen fluid viscous? If not, there are large filter units I've used to filter supernatants of large cultures to collect and analyze secreted proteins.
E.g. www.thermofisher.com/order/catalo...
February 10, 2025 at 7:27 PM
lycomorpha.bsky.social
Hmm. Time for some more small good things in a fkd up world:

1. Today we draw. Look at that temperature 😍

2. Made some really chuffing good madder lakes this week - from top down - red (alizarine), pink-brown, coral pink (it's not so orange IRL)
A screen cap from my phone of the weather forecast in my area, the main point being that it's 18 °C and cloudy right now, with a predicted high of 21, and a 50% chance of rain. We also have a west south westerly breeze, maximum gusts prediction is 20 MPH, and low pollen count. Temperatures for the rest of of the week are also in the low 20's. As I say that please imagine an angelic choir starts singing the Hallelujah chorus from Handel's Messiah. 3 different colours of freshly made pigment being filtered out of their colourful supernatants using coffee filters over large jars. To their right there's beakers with ladles in with the rest of the solution waiting to be filtered.
August 20, 2026 at 8:45 AM
iplantmicro.bsky.social
We are very happy to host Weronika and Natalia from Lodz University of Technology for their PhD internship! They will learn some of our protocols to identify key metabolites in their strains supernatants, welcome! 🇵🇱
@itqbnova.bsky.social
March 16, 2026 at 12:22 PM
plosbiology.org
Archaea-on-bacteria action! @romainstrock.bsky.social @tobiaswarnecke.bsky.social &co show that many #archaea encode #peptidoglycan hydrolases, which specifically target #bacterial cell walls, experimentally confirming the killing capacity of 2 of these enzymes @plosbiology.org 🧪 plos.io/4lrJBBa
Left: Results of spotting various supernatants onto lawns of Halalkalibacterium halodurans, Phycicoccus endophyticus, and Virgibacillus salexigens. a,b: top fraction (>3 kDa) of supernatant from Haloferax volcanii expressing an intact (a) or catalytic mutant (b) version of Woldo; c,d: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing an intact (c) or catalytic mutant (d) version of Woldo; e: unfiltered supernatant from Halogranum salarium B-1; f,g: top fraction (>3 kDa) of supernatant from H. volcanii expressing (f) Danwoldo or (g) and empty plasmid control; h,i: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing (h) Danwoldo or (i) and empty plasmid control. Top right: Structural overlay of zoocin A from Streptococcus zooepidemicus (UniProt ID: O54308) and Woldo, a candidate PGH from Halogranum salarium B-1 (UniProt ID: J2ZZK6), highlighting homologous M23 domains but divergent cell wall binding domains. TRD: target recognition domain. PG: peptidoglycan. Bottom right: Images of bacterial cells following exposure to H. volcanii supernatants expressing Woldo, Danwoldo or the control (as above). Bacteria are stained using LIVE/DEAD BacLight bacterial viability staining kit with ingress of red dye into bacterial cells indicative of cells with terminally compromised cell wall integrity.
August 15, 2025 at 1:54 PM
dmcilroy.bsky.social
Here's the neutralization assay on the supernatants:
Graph with three curves on it
February 22, 2025 at 1:36 PM
gregmedlock.bsky.social
Tried Edison Analysis (not Kosmos) on two tasks and was blown away:

1. Uploaded 96 high-res NMR spectra of bacterial supernatants and asked for metabolite ID and quantification

2. Uploaded a bacterial genome and asked it to identify mobile genetic elements (more complex, but this is the gist)
sgrodriques.bsky.social Sam Rodriques @sgrodriques.bsky.social · Nov 5
Give it a try here: platform.edisonscientific.com

And read our paper: edisonscientific.com/kosmos-report

We have built this to accelerate science, and we think it's pretty neat. We are super excited to see what people think.
Edison Platform
AI Agents for Scientific Discovery
platform.edisonscientific.com
January 15, 2026 at 4:17 PM
psynubs.bsky.social
I think they’re confident they can suck young men in and those that voted NO and of course their stalwart cohort of cashed up supernatants, then theres Gina’s money and Murdochs MSM and the dills who can’t see a truth from blatant lie.
February 4, 2025 at 12:09 AM
sailorrooscout.bsky.social
small tonsil spheres, each a “mini-me” that acts just like a lymph node- the ideal environment for antibody manufacturing. Sure enough, B-cells in these organoids that recognized any of the four conjoined hemagglutinin molecules swallowed the whole matrix and, potentially, displayed bits of all four
Supplementary figure 11: The aggregates in IIV are typically homotypic, and interactions between HAs from different subtypes or with non-HA proteins are limited. The binding of IIV to a panel of antibodies specific to HA and non-HA proteins was monitored using biolayer interferometry (BLI). Shown are representative traces of direct antibody binding to IIV (solid circle, solid line). The plot also shows the overlay of antibody binding to IIV captured by the indicated antibody (sequential binding, open circle and dashed line): (A) 5A7, or (B) C585. The binding experiments were repeated at least twice using two independent vials of the seasonal vaccine formulation. Supplementary figure 12: Design and characterization of the covalent coupled heterologous influenza antigen. (A) HAs from distinct influenza subtypes were coupled using the sortase ligation system. To facilitate this, a pentaglycine tag (G5) was engineered at the N-terminus of each subunit of the scaffold, lumazine synthase (LS), a self-assembling 60-mer. As shown, purified full-length HAs with a C-terminus LPET motif were iteratively conjugated to the functional scaffold (G5LS). (B) SDS-PAGE and gaussian peak fit of the size-exclusion chromatograms is shown for the purified scaffold and full-length HAs. Lane 1: G5LS. The protein dissociates into monomers (18.3-kDa) after boiling and migrates at the corresponding size on a protein denaturing gel. Lanes 2-5: Purified HAs; H1, H3, B(vi) and B(ya). The trimeric, full-length HAs dissociate upon boiling and migrate at the corresponding monomeric size (~62.5-kDa). (C) Supplementary figure 13: Internalization of intact coupled heterologous antigen in an engineered B cell line. (A) B cells internalize cognate antigen via the process of B cell receptor (BR)-mediated endocytosis preceding processing and presentation of antigen-derived peptides on MHC-II. We generated a B cell line expressing a membrane-bound immunoglobulin corresponding to the H1 HA-specific BCR (CR6261) This cell line binds (i) Homologous H1 HA Supplementary figure 14: The antibody response to full-length HAs in tonsil organoids after the indicated stimulation was determined by ELISA. The anti-HA Ig binding AUC of organoid culture supernatants against the distinct influenza subtypes, following the indicated antigen stimulation, is shown for each donor (n = 10).
January 2, 2025 at 5:08 PM
sailorrooscout.bsky.social
subtypes, thus recruiting far more helper T-cells to kick-start their activation. The result was solid antibody responses to all four influenza strains. There is considerable concern about a viral strain that could cause the next devastating pandemic: namely avian or “bird flu,” which recently has
Supplementary figure 15: The antibody response to HA head and stem-domains after stimulation of tonsil organoids with the coupled heterologous influenza antigen was measured by ELISA (n = 10): (A) H1, (B) H3, (C) B(vi), and (D) B(ya). ELISA binding curves for a representative donor are shown. The Ig binding AUC is shown using box plots. P-values were calculated using a two-tailed paired t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and not significant (n.s.). Supplementary figure 16: Strain-specific response in tonsil organoid culture supernatants against full-length HAs, after depletion of antibodies using H1 HA were measured by ELISA: (A) H1, (B) H3, (C) B(vi), and (D) B(ya). The organoids were stimulated the coupled heterologous antigen (n = 6). The anti-HA Ig binding AUC is shown using box plots. P-values were determined using a two-tailed Wilcoxon test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and not significant (n.s.). Supplementary figure 17: The antibody response to the HA stem-domain after stimulation of tonsil organoids with IIV or the coupled heterologous influenza antigen was measured by ELISA (n = 10): (A) H1, (B) H3, (C) B(vi), and (D) B(ya). The anti-HA stem Ig binding AUC is shown using box plots. P-values were calculated using a two-tailed paired t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and not significant (n.s.). Supplementary figure 18: The antibody response to full-length HAs after stimulation of tonsil organoids with the indicated antigens was measured by ELISA (n = 10): (A) H1, (B) H3, (C) B(vi), and (D) B(ya). The anti-HA Ig binding AUC is shown using box plots. Adjusted p-values were calculated using one-way ANOVA with Dunnett's test for pairwise comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Supplementary figure 19: Gating strategy and representative flow cytometry plots showing CD4+ Th cell activation (CD38+) after stimulation of tonsil organoids with influenza antigens. Supplementary figure 20: The CD4+ Th response to the full-length scaffold and HA proteins in tonsil organoids after stimulation with the coupled heterologous HA antigen was measured by flow cytometry (n = 6). The response to the antigen components without stimulation is also shown. (A) Frequency of activated CD4+ Th (CD137+OX40+) cells is plotted using box plots. (B) The scaffold- and HA-specific fold-change in response to stimulation is shown using box plots. P-values were calculated using two-tailed Wilcoxon test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Supplementary figure 21: Gating strategy and representative flow cytometry plots showing the staining of antigen-specific non-naïve B cells using H5 HA- and H1 HA-spheromers after stimulation of tonsil organoids with influenza antigens.
January 2, 2025 at 5:11 PM
cp-cellhostmicrobe.bsky.social
Microbiota-derived valeric acid blunts C. parapsilosis

Valeric acid levels inversely correlate w/Candida burden in allo-HCT patients. VA induces loss of intracellular C. parapsilosis pH homeostasis & VA derivatives suppress C. parapsilosis growth in vivo
www.cell.com/cell-host-mi...
Intracellular acidification by microbiota-derived valeric acid facilitates trans-kingdom ecology limiting Candida parapsilosis colonization
By screening Lachnospiraceae supernatants using SHAP analysis, Yasuma-Mitobe et al. identify bacteria-derived valeric acid (VA) as an inhibitor of C. parapsilosis growth. VA levels inversely correlate...
www.cell.com
June 5, 2026 at 4:04 PM