very unusual - a tRNA regulated anion channel
December 18, 2025 at 5:26 PM
very unusual - a tRNA regulated anion channel
Fantastic, comprehensive, useful review if you care about RNA modifications in bacteria: @asm.org @csdetweiler.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Compendium of RNA modifications for bacterial stress adaptation | Microbiology and Molecular Biology Reviews
SUMMARYRNA modifications in the tRNA, rRNA, and mRNA constitute a widespread layer of post-transcriptional
gene regulation, commonly known as the epitranscriptome. In bacteria, tRNA and rRNA
modificat...
journals.asm.org
September 23, 2026 at 2:59 AM
Fantastic, comprehensive, useful review if you care about RNA modifications in bacteria: @asm.org @csdetweiler.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
TRNA INVENTS UBEREATS
July 24, 2026 at 3:34 AM
TRNA INVENTS UBEREATS
DNA/RNA transcription translation jokes
August 18, 2026 at 5:44 PM
DNA/RNA transcription translation jokes
📣 Final version of our @elife.bsky.social article is now online! We show how the human tRNA methyltransferase TRMT1 is recognized, cleaved, and inactivated by the SARS-CoV-2 main protease to disrupt host tRNA modification!
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease
The SARS-CoV-2 main protease specifically cleaves a conserved sequence in the human tRNA modifying enzyme TRMT1, resulting in reduced tRNA binding and the complete loss of TRMT1-mediated tRNA methyltr...
elifesciences.org
January 9, 2025 at 2:36 PM
📣 Final version of our @elife.bsky.social article is now online! We show how the human tRNA methyltransferase TRMT1 is recognized, cleaved, and inactivated by the SARS-CoV-2 main protease to disrupt host tRNA modification!
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
⭐NAR Breakthrough!⭐
🧬 Sun et al. just dropped a high-throughput method to track all #tRNA #modifications across cells & tissues using #massspec.
Read the full article here: doi.org/10.1093/nar/...
#NARBreakthrough #RNA #tRNA #epitranscriptomics #systemsbiology #therapeuticdiscovery.
🧬 Sun et al. just dropped a high-throughput method to track all #tRNA #modifications across cells & tissues using #massspec.
Read the full article here: doi.org/10.1093/nar/...
#NARBreakthrough #RNA #tRNA #epitranscriptomics #systemsbiology #therapeuticdiscovery.
tRNA modification profiling reveals epitranscriptome regulatory networks in Pseudomonas aeruginosa
Abstract. Transfer RNA (tRNA) modifications have emerged as critical post-transcriptional regulators of gene expression affecting diverse biological and di
doi.org
July 28, 2025 at 8:12 AM
⭐NAR Breakthrough!⭐
🧬 Sun et al. just dropped a high-throughput method to track all #tRNA #modifications across cells & tissues using #massspec.
Read the full article here: doi.org/10.1093/nar/...
#NARBreakthrough #RNA #tRNA #epitranscriptomics #systemsbiology #therapeuticdiscovery.
🧬 Sun et al. just dropped a high-throughput method to track all #tRNA #modifications across cells & tissues using #massspec.
Read the full article here: doi.org/10.1093/nar/...
#NARBreakthrough #RNA #tRNA #epitranscriptomics #systemsbiology #therapeuticdiscovery.
Interested in direct tRNA-seq by Nanopore? We developed a new RNA-based barcoding aproach for seq parallelization; it is also cost-saving. Scripts available on github. #tRNA #Nanopore
track.smtpsendmail.com/9032119/c?p=...
track.smtpsendmail.com/9032119/c?p=...
ADAM-tRNA-seq: an optimized approach for demultiplexing and enhanced hierarchal mapping in direct tRNA sequencing
Abstract. Transfer RNAs (tRNAs) play an essential role in protein synthesis and cellular homeostasis, and their dysregulation is associated with various hu
track.smtpsendmail.com
January 27, 2026 at 10:14 PM
Interested in direct tRNA-seq by Nanopore? We developed a new RNA-based barcoding aproach for seq parallelization; it is also cost-saving. Scripts available on github. #tRNA #Nanopore
track.smtpsendmail.com/9032119/c?p=...
track.smtpsendmail.com/9032119/c?p=...
In First Release @science.org this week
A hypoxia-responsive tRNA-derived small RNA confers renal protection through RNA autophagy | Science www.science.org/doi/10.1126/...
A hypoxia-responsive tRNA-derived small RNA confers renal protection through RNA autophagy | Science www.science.org/doi/10.1126/...
A hypoxia-responsive tRNA-derived small RNA confers renal protection through RNA autophagy
Transfer RNA-derived small RNAs (tsRNAs, or tDRs) perform a range of cellular functions. Here, we showed that a hypoxia-induced tDR, derived from the 3′ end of tRNA-Asp-GTC (tRNA-Asp-GTC-3′tDR), activ...
www.science.org
July 21, 2025 at 4:02 PM
In First Release @science.org this week
A hypoxia-responsive tRNA-derived small RNA confers renal protection through RNA autophagy | Science www.science.org/doi/10.1126/...
A hypoxia-responsive tRNA-derived small RNA confers renal protection through RNA autophagy | Science www.science.org/doi/10.1126/...
New preprint on our method aa-tRNA-seq: chemically capturing aminoacylated tRNA molecules & pulling them through nanopores. It’s been a blast working with my collaborator Aleks Radakovic to take this from idea to promising pilot data to some looking exciting biology to chase down in future work.
Here for my first post. Our new approach to study tRNA aminoacylation by nanopore sequencing. Started as a napkin sketch ~1 year ago! This was a fun one, congrats to @laurakwhite.bsky.social and Aleks.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Nanopore sequencing of intact aminoacylated tRNAs
Transfer RNAs (tRNA) are decorated during biogenesis with a variety of modifications that modulate their stability, aminoacylation, and decoding potential during translation. The complex landscape of ...
www.biorxiv.org
November 19, 2024 at 3:17 AM
New preprint on our method aa-tRNA-seq: chemically capturing aminoacylated tRNA molecules & pulling them through nanopores. It’s been a blast working with my collaborator Aleks Radakovic to take this from idea to promising pilot data to some looking exciting biology to chase down in future work.
Sustainable regeneration of 20 aminoacyl-tRNA synthetases in a reconstituted system toward self-synthesizing artificial systems
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Sustainable regeneration of 20 aminoacyl-tRNA synthetases in a reconstituted system toward self-synthesizing artificial systems
An in vitro system is capable of sustainably regenerating 20 aminoacyl-tRNA synthetases toward artificial cells.
www.science.org
April 5, 2025 at 8:17 PM
Sustainable regeneration of 20 aminoacyl-tRNA synthetases in a reconstituted system toward self-synthesizing artificial systems
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
It is finally (FINALLY) out! We characterized Kathy Collin's super cool OTTR protocol for tRNA and tRF cloning, showing that it is comparable (mim-tRNA-seq, maybe nano-tRNA -seq and LIDAR) or superior (everything else) to the best protocols in the literature.
elifesciences.org/articles/77616
elifesciences.org/articles/77616
Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTR
elifesciences.org
April 28, 2025 at 12:33 AM
It is finally (FINALLY) out! We characterized Kathy Collin's super cool OTTR protocol for tRNA and tRF cloning, showing that it is comparable (mim-tRNA-seq, maybe nano-tRNA -seq and LIDAR) or superior (everything else) to the best protocols in the literature.
elifesciences.org/articles/77616
elifesciences.org/articles/77616
Our new paper maps the tRNA modification landscape in Vibrio cholerae! 💫
We describe differences from E. coli and discuss links to decoding of stress-related codons 🦠
Huge thanks to amazing co-authors and collaborators!
@plos.org #rnasky #microsky #tRNAmodifications
We describe differences from E. coli and discuss links to decoding of stress-related codons 🦠
Huge thanks to amazing co-authors and collaborators!
@plos.org #rnasky #microsky #tRNAmodifications
The tRNA epitranscriptomic landscape and RNA modification enzymes in Vibrio cholerae
Author summary This study charts the first genome-wide map of transfer RNA (tRNA) modifications in the cholera pathogen, Vibrio cholerae, revealing how chemical marks on tRNAs shape translation and st...
journals.plos.org
November 3, 2025 at 11:58 PM
Our new paper maps the tRNA modification landscape in Vibrio cholerae! 💫
We describe differences from E. coli and discuss links to decoding of stress-related codons 🦠
Huge thanks to amazing co-authors and collaborators!
@plos.org #rnasky #microsky #tRNAmodifications
We describe differences from E. coli and discuss links to decoding of stress-related codons 🦠
Huge thanks to amazing co-authors and collaborators!
@plos.org #rnasky #microsky #tRNAmodifications
Better not let them find out about tRNA…
February 7, 2025 at 4:56 PM
Better not let them find out about tRNA…
Preprint alert 🚨: We built a CRISPRi screen for a part of the genome that is unusually awkward to perturb: the RNA Pol III transcriptome.
Hundreds of tRNA genes, lots of sequence similarity, plenty of redundancy...
Turns out the biology had a few surprises for us 👇
www.biorxiv.org/content/10.6...
Hundreds of tRNA genes, lots of sequence similarity, plenty of redundancy...
Turns out the biology had a few surprises for us 👇
www.biorxiv.org/content/10.6...
Targeted CRISPRi screening reveals unexpected resilience across the RNA polymerase III transcriptome
Increased RNA polymerase III (Pol III) activity and tRNA abundance are widely linked to cancer cell growth, yet the functional requirement for individual Pol III genes and core components remains uncl...
www.biorxiv.org
September 28, 2026 at 9:07 AM
Preprint alert 🚨: We built a CRISPRi screen for a part of the genome that is unusually awkward to perturb: the RNA Pol III transcriptome.
Hundreds of tRNA genes, lots of sequence similarity, plenty of redundancy...
Turns out the biology had a few surprises for us 👇
www.biorxiv.org/content/10.6...
Hundreds of tRNA genes, lots of sequence similarity, plenty of redundancy...
Turns out the biology had a few surprises for us 👇
www.biorxiv.org/content/10.6...
mRNA, tRNA, rRNA? Make up your mind!
January 23, 2025 at 4:15 PM
mRNA, tRNA, rRNA? Make up your mind!
Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution: Trends in Biochemical Sciences www.cell.com/trends/bioch...
Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution
Chemical modifications of rRNA, tRNA, and mRNA play key roles in protein synthesis
by affecting the structure of these RNAs, by modulating decoding, and by influencing
ribosomal efficiency. Recent adv...
www.cell.com
October 24, 2025 at 8:28 AM
Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution: Trends in Biochemical Sciences www.cell.com/trends/bioch...
Excited to share our new work on the earliest steps of Pol-III transcription: We provide structural and kinetic insights into tRNA promoter engagement by yeast transcription factor TFIIIC !
Cool collaboration with the Müller and Eustermann Labs @embl.org !
academic.oup.com/nar/advance-...
Cool collaboration with the Müller and Eustermann Labs @embl.org !
academic.oup.com/nar/advance-...
Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC
Abstract. Transcription of transfer RNA (tRNA) genes by RNA polymerase (Pol) III requires the general transcription factor IIIC (TFIIIC), which recognizes
academic.oup.com
December 11, 2024 at 7:39 AM
Excited to share our new work on the earliest steps of Pol-III transcription: We provide structural and kinetic insights into tRNA promoter engagement by yeast transcription factor TFIIIC !
Cool collaboration with the Müller and Eustermann Labs @embl.org !
academic.oup.com/nar/advance-...
Cool collaboration with the Müller and Eustermann Labs @embl.org !
academic.oup.com/nar/advance-...
Today's holiday ☕ reading is this 2024 paper on PARIS, a bacterial defense system shaped like a literal propeller that responds to viral infection by hitting the cellular self-destruct button - cleaving an essential tRNA. www.nature.com/articles/s41...
A virally encoded tRNA neutralizes the PARIS antiviral defence system - Nature
Structural and functional studies reveal how viral proteins trigger the phage antirestriction induced system (PARIS) to degrade host tRNA and how viral tRNAs suppress the PARIS nuclease and thereby ov...
www.nature.com
December 24, 2024 at 3:18 PM
Today's holiday ☕ reading is this 2024 paper on PARIS, a bacterial defense system shaped like a literal propeller that responds to viral infection by hitting the cellular self-destruct button - cleaving an essential tRNA. www.nature.com/articles/s41...
Happy to share our new review in Royal Society Open Biology 🎉
tRNA-modifying enzymes in bacterial stress adaptation:
How tRNA mods rewire translation under oxidative + antibiotic stress (MoTTs, moonlighting, therapy angles).
Read: doi.org/10.1098/rsob...
#RNAsky #microsky #AMR #RNAmodifications
tRNA-modifying enzymes in bacterial stress adaptation:
How tRNA mods rewire translation under oxidative + antibiotic stress (MoTTs, moonlighting, therapy angles).
Read: doi.org/10.1098/rsob...
#RNAsky #microsky #AMR #RNAmodifications
tRNA-modifying enzymes in bacterial stress adaptation | Open Biology
Transfer RNAs (tRNAs) and their modifications are central to bacterial translation
and physiology, yet their roles in stress adaptation remain underexplored. While extensively
studied in eukaryotes, a...
doi.org
October 8, 2025 at 2:02 PM
Happy to share our new review in Royal Society Open Biology 🎉
tRNA-modifying enzymes in bacterial stress adaptation:
How tRNA mods rewire translation under oxidative + antibiotic stress (MoTTs, moonlighting, therapy angles).
Read: doi.org/10.1098/rsob...
#RNAsky #microsky #AMR #RNAmodifications
tRNA-modifying enzymes in bacterial stress adaptation:
How tRNA mods rewire translation under oxidative + antibiotic stress (MoTTs, moonlighting, therapy angles).
Read: doi.org/10.1098/rsob...
#RNAsky #microsky #AMR #RNAmodifications
Our paper on 🦠queuosine 34 modification of tRNA tyrosine and antibiotic response is finally out in its final form in @eLife ... and quite different from the preprint version. Many thanks to reviewers, editors and kudos to all authors🙏 elifesciences.org/articles/96317
Aminoglycoside tolerance in Vibrio cholerae engages translational reprogramming associated with queuosine tRNA modification
tRNA Q34 modification impacts tyrosine codon decoding and leads to proteome reprogramming in response to antibiotic stress in Vibrio cholerae.
elifesciences.org
January 12, 2025 at 11:07 AM
Our paper on 🦠queuosine 34 modification of tRNA tyrosine and antibiotic response is finally out in its final form in @eLife ... and quite different from the preprint version. Many thanks to reviewers, editors and kudos to all authors🙏 elifesciences.org/articles/96317
Our paper is out! Hiding in plain sight among Cas12a nucleases, Cas12a3 cleaves not its RNA target but the 3′ ends of tRNA. Huge thanks to all who made this possible, especially the Beisel lab, Biao & Dirk for the structure, & @sebastianglatt.bsky.social for all things tRNA. doi.org/10.1038/s415...
RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity - Nature
Cas12a3 nucleases constitute a distinct clade of type V CRISPR–Cas bacterial immune systems that preferentially cleave the 3′ tails of tRNAs after recognition of target RNA to induce growth arres...
www.nature.com
January 7, 2026 at 4:15 PM
Our paper is out! Hiding in plain sight among Cas12a nucleases, Cas12a3 cleaves not its RNA target but the 3′ ends of tRNA. Huge thanks to all who made this possible, especially the Beisel lab, Biao & Dirk for the structure, & @sebastianglatt.bsky.social for all things tRNA. doi.org/10.1038/s415...
Out now in @science.org
Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis | Science www.science.org/doi/10.1126/...
Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis | Science www.science.org/doi/10.1126/...
Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis
Suppressor transfer RNAs (sup-tRNAs) can rescue disease-causing nonsense mutations by promoting readthrough of premature termination codons (PTCs). Their clinical translation is limited by suboptimal ...
www.science.org
August 28, 2026 at 9:38 AM
Out now in @science.org
Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis | Science www.science.org/doi/10.1126/...
Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis | Science www.science.org/doi/10.1126/...
WarpDemuX-tRNA: barcode multiplexing for nanopore tRNA sequencing #rnasky💫👏 academic.oup.com/nar/article/...
WarpDemuX-tRNA: barcode multiplexing for nanopore tRNA sequencing
Abstract. Transfer RNA (tRNA) plays an essential role in protein translation, and tRNA modifications are important to their function. Recently, nanopore di
academic.oup.com
September 11, 2025 at 7:39 AM
WarpDemuX-tRNA: barcode multiplexing for nanopore tRNA sequencing #rnasky💫👏 academic.oup.com/nar/article/...