King Cada
@kingcada.bsky.social
Studying the molecular sociology between cellular membranes and organelles, in neurons, with the proteins that shape and transport them
Reposted by King Cada
He is a remarkably gifted scientist but he is also a welcome reminder to me that the best ideas in a lab often come from the imagination of trainees. Yiu can read about his integrin work here: www.science.org/doi/10.1126/... and in a preprint we hope to post later this year (watch this space)
Molecular exaptation by the integrin αI domain
An ancient protein domain insertion seamlessly preserved conformational signaling while providing a novel surface for adaptation.
www.science.org
July 16, 2026 at 3:27 AM
He is a remarkably gifted scientist but he is also a welcome reminder to me that the best ideas in a lab often come from the imagination of trainees. Yiu can read about his integrin work here: www.science.org/doi/10.1126/... and in a preprint we hope to post later this year (watch this space)
Reposted by King Cada
Annexin A11 and TDP-43: core players in neurodegeneration pubmed.ncbi.nlm.nih.gov/42414528/ #cryoem
July 8, 2026 at 11:47 AM
Annexin A11 and TDP-43: core players in neurodegeneration pubmed.ncbi.nlm.nih.gov/42414528/ #cryoem
Reposted by King Cada
An evolutionarily conserved, short linear actin filament-binding motif (SFM), present in >100 proteins.
Great collaboration with the Windhorst and Marlovits labs. Happy to have contributed, by using macrophage podosomes as a readout.
Publication in Nat Cell Biol:
www.nature.com/articles/s41...
Great collaboration with the Windhorst and Marlovits labs. Happy to have contributed, by using macrophage podosomes as a readout.
Publication in Nat Cell Biol:
www.nature.com/articles/s41...
July 6, 2026 at 11:20 AM
An evolutionarily conserved, short linear actin filament-binding motif (SFM), present in >100 proteins.
Great collaboration with the Windhorst and Marlovits labs. Happy to have contributed, by using macrophage podosomes as a readout.
Publication in Nat Cell Biol:
www.nature.com/articles/s41...
Great collaboration with the Windhorst and Marlovits labs. Happy to have contributed, by using macrophage podosomes as a readout.
Publication in Nat Cell Biol:
www.nature.com/articles/s41...
Reposted by King Cada
Incredibly proud of Messi Haile @mehsehret.bsky.social, a founding member of the Ho Lab, for her beautiful work uncovering the inner workings of a central piece of the malaria parasite invasion machinery: the malarial moving junction, out today in @cellpress.bsky.social! tinyurl.com/4p3md2eb 🦠❄️🔬
June 30, 2026 at 5:13 PM
Incredibly proud of Messi Haile @mehsehret.bsky.social, a founding member of the Ho Lab, for her beautiful work uncovering the inner workings of a central piece of the malaria parasite invasion machinery: the malarial moving junction, out today in @cellpress.bsky.social! tinyurl.com/4p3md2eb 🦠❄️🔬
Reposted by King Cada
Phosphatidylserine and RhoB connect PI4P and PA #metabolism to maintain plasma #membrane identity. New study from Shiying Huang, Tamas Balla, Jeremy M. Baskin @jeremybaskin.bsky.social, @weillinstitute.bsky.social and colleagues:
rupress.org/jcb/article/...
#lipid
rupress.org/jcb/article/...
#lipid
July 3, 2026 at 1:45 PM
Phosphatidylserine and RhoB connect PI4P and PA #metabolism to maintain plasma #membrane identity. New study from Shiying Huang, Tamas Balla, Jeremy M. Baskin @jeremybaskin.bsky.social, @weillinstitute.bsky.social and colleagues:
rupress.org/jcb/article/...
#lipid
rupress.org/jcb/article/...
#lipid
Reposted by King Cada
With a motivating push from @christlet.bsky.social I made a new Fiji plugin, CurveMorph. The idea was to create a tool for kymograph generation along the dynamic (bending/shaking) filament shapes (microtubules, cilia, neurites, etc).
The logo was made using the plugin.
github.com/UU-cellbiolo...
The logo was made using the plugin.
github.com/UU-cellbiolo...
June 16, 2026 at 12:20 PM
With a motivating push from @christlet.bsky.social I made a new Fiji plugin, CurveMorph. The idea was to create a tool for kymograph generation along the dynamic (bending/shaking) filament shapes (microtubules, cilia, neurites, etc).
The logo was made using the plugin.
github.com/UU-cellbiolo...
The logo was made using the plugin.
github.com/UU-cellbiolo...
Reposted by King Cada
Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization?
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
Think globally, act locally: Redefining organellar membrane environments through cryo-electron tomography
Early enthusiasm for the “cellular revolution” in cryo-electron tomography (cryo-ET) was largely driven by the promise of resolving protein structures…
www.sciencedirect.com
June 16, 2026 at 6:08 PM
Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization?
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
Reposted by King Cada
In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn
June 11, 2026 at 6:02 PM
In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn
Glad to know I am not alone in feeling this.
It is outrageous that word still does not recognize the word “clathrin”
June 11, 2026 at 12:30 AM
Glad to know I am not alone in feeling this.
Reposted by King Cada
Very excited to be sharing our new paper, just out in Nature Cell Biology!
The big question we investigate: How do migrating cells put their front 🔴 and back 🔵 in the right place?
The big question we investigate: How do migrating cells put their front 🔴 and back 🔵 in the right place?
June 10, 2026 at 6:27 PM
Very excited to be sharing our new paper, just out in Nature Cell Biology!
The big question we investigate: How do migrating cells put their front 🔴 and back 🔵 in the right place?
The big question we investigate: How do migrating cells put their front 🔴 and back 🔵 in the right place?
Reposted by King Cada
In-cell structures visualize human pre-ribosome assembly in the nucleolus https://www.biorxiv.org/content/10.64898/2026.06.01.729245v1
June 2, 2026 at 1:30 PM
In-cell structures visualize human pre-ribosome assembly in the nucleolus https://www.biorxiv.org/content/10.64898/2026.06.01.729245v1
Reposted by King Cada
So excited to share our new paper out today in @nature.com - 'LASER couples damage sensing to ESCRT assembly for lysosome repair'. This was an amazing collaborative effort with Claire Goul during our time in @robzonculab.bsky.social : www.nature.com/articles/s41...
LASER couples damage sensing to ESCRT assembly for lysosome repair - Nature
ATG8 conjugation on damaged lysosomes triggers rapid assembly of a protein complex containing TFG, which directs lysosomal membrane repair by recruiting ESCRT proteins to sites of damage.
www.nature.com
June 3, 2026 at 5:26 PM
So excited to share our new paper out today in @nature.com - 'LASER couples damage sensing to ESCRT assembly for lysosome repair'. This was an amazing collaborative effort with Claire Goul during our time in @robzonculab.bsky.social : www.nature.com/articles/s41...
Reposted by King Cada
🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9
Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons
The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...
www.biorxiv.org
January 12, 2026 at 7:14 PM
🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9
Reposted by King Cada
Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
January 12, 2026 at 7:57 PM
Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Woot. Glad our work made it to your JC!
Incredible work from the Mizuno lab capturing the process of axon regeneration. On-grid axotomies!! www.nature.com/articles/s41...
Client Challenge
www.nature.com
January 8, 2026 at 8:35 PM
Woot. Glad our work made it to your JC!
Reposted by King Cada
Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇
www.nature.com/articles/s41...
More info 👇
Membrane editing with proximity labeling reveals regulators of lipid homeostasis - Nature Chemical Biology
Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.
www.nature.com
January 7, 2026 at 2:59 PM
Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇
www.nature.com/articles/s41...
More info 👇
Reposted by King Cada
Cryo-ET Reveals Distinct Gag Lattice Architectures in Virus-like Particles and Immature HIV-1 pubmed.ncbi.nlm.nih.gov/41427417/ #cryoem
December 24, 2025 at 6:01 AM
Cryo-ET Reveals Distinct Gag Lattice Architectures in Virus-like Particles and Immature HIV-1 pubmed.ncbi.nlm.nih.gov/41427417/ #cryoem
Reposted by King Cada
Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...
December 23, 2025 at 2:13 PM
Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...
Reposted by King Cada
Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
December 19, 2025 at 4:45 PM
Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
Reposted by King Cada
IsoNet2 determines cellular structures at submolecular resolution without averaging www.biorxiv.org/content/10.64898/2025.12.09.693325v1 #cryoem
December 12, 2025 at 11:42 AM
IsoNet2 determines cellular structures at submolecular resolution without averaging www.biorxiv.org/content/10.64898/2025.12.09.693325v1 #cryoem
Reposted by King Cada
This week, check out a new #Primer on #cryogenic electron tomography (cryo-ET) used for visualizing 3D structures of cells with sub-nanometer resolution. 🔬🧫
www.nature.com/articles/s43...
www.nature.com/articles/s43...
Correlative cryogenic light and electron tomography of eukaryotic cells - Nature Reviews Methods Primers
Cryogenic electron tomography provides unparalleled insights into cell biology and disease. When combined with light microscopy, it enables precise localization of regions of interest and direct corre...
www.nature.com
December 11, 2025 at 3:54 PM
This week, check out a new #Primer on #cryogenic electron tomography (cryo-ET) used for visualizing 3D structures of cells with sub-nanometer resolution. 🔬🧫
www.nature.com/articles/s43...
www.nature.com/articles/s43...
Reposted by King Cada
Excited to share our new preprint in collaboration with Ahmet Yildiz's lab. Check out how our team uncovers a novel binding footprint and motor regulation mechanism for MAP9 Congrats to Burak Cetin and @aryantaheri.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 18, 2025 at 10:43 PM
Excited to share our new preprint in collaboration with Ahmet Yildiz's lab. Check out how our team uncovers a novel binding footprint and motor regulation mechanism for MAP9 Congrats to Burak Cetin and @aryantaheri.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Our lab’s paper spearheaded by Keni and pushed to completion (and satisfaction by the reviewers) by Satish is finally out www.nature.com/articles/s41.... Was so fortunate to contribute to this project and learned a lot in both neuronal cell biology and cryoET. Also, Satish is on the job market!
November 12, 2025 at 5:35 PM
Our lab’s paper spearheaded by Keni and pushed to completion (and satisfaction by the reviewers) by Satish is finally out www.nature.com/articles/s41.... Was so fortunate to contribute to this project and learned a lot in both neuronal cell biology and cryoET. Also, Satish is on the job market!
Reposted by King Cada
ICYMI: New online: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
Nature Structural & Molecular Biology, Published online: 06 November 2025; doi:10.1038/s41594-025-01693-4Teng and Zeng et al. use cryo-electron microscopy to show that the crescent scaffold of KICSTOR anchors GATOR1 to lysosomes and disruption of the interaction causes mTORC1 hyperactivation and TFE3 mislocalization. KICSTOR enables nutrient-dependent mTORC1 regulation by binding anionic lipids for lysosomal targeting.
go.nature.com
November 7, 2025 at 11:45 AM
ICYMI: New online: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
Reposted by King Cada
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
November 7, 2025 at 9:50 PM
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...