Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Hello Bsky folks - here is a paper that we published earlier this year - great collaboration with the Bellen lab, Hyung-lok Chung, @okanca.bsky.social and others. Have a look if you're interested in Cdk8/19, mitochondria, Parkinsonism and Drosophila!
Liao, J. Z., Chung, H. L., Shih, C., Wong, K. K. L., ...., Sew, K., Bellen, H. J., Verheyen, E. M. (2024). Cdk8/CDK19 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency in Drosophila. Nat Commun, 15(1):3326 www.nature.com/articles/s41...
Cdk8/CDK19 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency in Drosophila - Nature Communications
Mitochondrial fission, performed by Drp1, is carefully regulated, particularly in neurons. Here, the authors examine Drosophila Cdk8/CDK19 function in mitochondrial fission and uncover a role phosphor...
www.nature.com
November 20, 2024 at 5:51 AM
Hello Bsky folks - here is a paper that we published earlier this year - great collaboration with the Bellen lab, Hyung-lok Chung, @okanca.bsky.social and others. Have a look if you're interested in Cdk8/19, mitochondria, Parkinsonism and Drosophila!
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila
Read this Research Article by Xiao Li, Jun-Yuan Ji @tulaneu.bsky.social and colleagues:
https://doi.org/10.1242/dev.203111
Read this Research Article by Xiao Li, Jun-Yuan Ji @tulaneu.bsky.social and colleagues:
https://doi.org/10.1242/dev.203111
December 5, 2024 at 10:51 AM
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila
Read this Research Article by Xiao Li, Jun-Yuan Ji @tulaneu.bsky.social and colleagues:
https://doi.org/10.1242/dev.203111
Read this Research Article by Xiao Li, Jun-Yuan Ji @tulaneu.bsky.social and colleagues:
https://doi.org/10.1242/dev.203111
Excellent presentation by Elizabeth Bonner @ebonner.bsky.social on understanding the biological consequences of CDK8 mis-splicing in SF3B1-mutant MDS. #ASH24
December 8, 2024 at 1:25 AM
Excellent presentation by Elizabeth Bonner @ebonner.bsky.social on understanding the biological consequences of CDK8 mis-splicing in SF3B1-mutant MDS. #ASH24
Hey! I'm Victor. I'm a cell and cancer biologist. My lab mainly studies CDK8/19, cell cycle regulation, and new anti-cancer drug candidates. I also love history and DnD, so don't be surprised if I follow you. I'm Russian (sorry) with family in the US, so there is occasional politics.
October 26, 2024 at 9:20 AM
Hey! I'm Victor. I'm a cell and cancer biologist. My lab mainly studies CDK8/19, cell cycle regulation, and new anti-cancer drug candidates. I also love history and DnD, so don't be surprised if I follow you. I'm Russian (sorry) with family in the US, so there is occasional politics.
#cryoEM Look at this !
Structure of human transcriptional Mediator complex with the CDK8 kinase module
www.rcsb.org/structure/8TQW
www.cell.com/molecular-ce...
Structure of human transcriptional Mediator complex with the CDK8 kinase module
www.rcsb.org/structure/8TQW
www.cell.com/molecular-ce...
October 9, 2024 at 8:03 AM
#cryoEM Look at this !
Structure of human transcriptional Mediator complex with the CDK8 kinase module
www.rcsb.org/structure/8TQW
www.cell.com/molecular-ce...
Structure of human transcriptional Mediator complex with the CDK8 kinase module
www.rcsb.org/structure/8TQW
www.cell.com/molecular-ce...
Read this Cancer Discovery research article, "CDK8 Inhibition Releases the Muscle Differentiation Block in Fusion-Driven Alveolar Rhabdomyosarcoma," presented by Kimberly Stegmaier at #AACRpediatric26. buff.ly/6Fl9QqH
September 23, 2026 at 7:30 PM
Read this Cancer Discovery research article, "CDK8 Inhibition Releases the Muscle Differentiation Block in Fusion-Driven Alveolar Rhabdomyosarcoma," presented by Kimberly Stegmaier at #AACRpediatric26. buff.ly/6Fl9QqH
Now in Cancer Discovery: two studies showing that CDK8 inhibitors act by trapping Mediator on chromatin & that cancers w/driver oncogenes acting as dosage-sensitive transcription factors (e.g. aRMS, Ewing sarcoma) are vulnerable to impairment of transcriptional regulatory complex dynamics:
July 21, 2026 at 3:30 PM
Now in Cancer Discovery: two studies showing that CDK8 inhibitors act by trapping Mediator on chromatin & that cancers w/driver oncogenes acting as dosage-sensitive transcription factors (e.g. aRMS, Ewing sarcoma) are vulnerable to impairment of transcriptional regulatory complex dynamics:
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila url: journals.biologists.com/dev/article/...
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila | Development | The Company of Biologists
journals.biologists.com
September 9, 2025 at 7:41 AM
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila url: journals.biologists.com/dev/article/...
CDK8 Inhibition Releases the Muscle Differentiation Block in Fusion-driven Alveolar Rhabdomyosarcoma - by Susu Zhang, Kathleen Engel, Corinne Linardic, Kim Stegmaier, and colleagues @danafarber.bsky.social @broadinstitute.org doi.org/10.1158/2159...
CDK8 Inhibition Releases the Muscle Differentiation Block in Fusion-driven Alveolar Rhabdomyosarcoma
Abstract. Alveolar rhabdomyosarcoma (aRMS) is a fusion-driven pediatric cancer with poor survival and limited therapeutic options. To uncover novel vulnerabilities, we employed complex-based analysis ...
doi.org
July 21, 2026 at 3:30 PM
CDK8 Inhibition Releases the Muscle Differentiation Block in Fusion-driven Alveolar Rhabdomyosarcoma - by Susu Zhang, Kathleen Engel, Corinne Linardic, Kim Stegmaier, and colleagues @danafarber.bsky.social @broadinstitute.org doi.org/10.1158/2159...
Funded postdoctoral position in our lab @SFU_Science working on Cdk8/CDK19 function using Drosophila models. verheyenlab.weebly.com/uploads/4/7/...
December 8, 2023 at 10:45 PM
Funded postdoctoral position in our lab @SFU_Science working on Cdk8/CDK19 function using Drosophila models. verheyenlab.weebly.com/uploads/4/7/...
CDK8 Inhibition Induces Mediator Trapping and Impairment of the EWSR1-FLI1 Transcriptional Program in Ewing Sarcoma - by James McGinnis, Shuai Wang, David MacFadden, and colleagues doi.org/10.1158/2159...
CDK8 inhibition induces Mediator trapping and impairment of the EWSR1::FLI1 transcriptional program in Ewing sarcoma.
Abstract. Ewing sarcoma is characterized by a chromosomal translocation resulting in the fusion protein EWSR1::FLI1. We utilize endogenous EWSR1::FLI1 target gene reporters in patient-derived cell lin...
doi.org
July 21, 2026 at 3:30 PM
CDK8 Inhibition Induces Mediator Trapping and Impairment of the EWSR1-FLI1 Transcriptional Program in Ewing Sarcoma - by James McGinnis, Shuai Wang, David MacFadden, and colleagues doi.org/10.1158/2159...
Ever wondered how plants breathe and manage water loss? 🌿 This depends on tiny pores called stomata. A new study reveals how a key regulator, CDK8, ensures these pores form in the right places, connecting leaf growth to function.
Curious to learn more?
onlinelibrary.wiley.com/doi/10.1111/...
Curious to learn more?
onlinelibrary.wiley.com/doi/10.1111/...
CDK8 of the mediator kinase module connects leaf development to the establishment of correct stomata patterning by regulating the levels of the transcription factor SPEECHLESS (SPCH)
The components of the mediator kinase module are highly conserved across eukaryotic lines and cyclin-dependent kinase 8 (CDK8) is essential for correct cell proliferation and development. We show her...
onlinelibrary.wiley.com
November 28, 2024 at 7:38 AM
Ever wondered how plants breathe and manage water loss? 🌿 This depends on tiny pores called stomata. A new study reveals how a key regulator, CDK8, ensures these pores form in the right places, connecting leaf growth to function.
Curious to learn more?
onlinelibrary.wiley.com/doi/10.1111/...
Curious to learn more?
onlinelibrary.wiley.com/doi/10.1111/...
Read this Cancer Discovery research article, "CDK8 Inhibition Induces Mediator Trapping and Impairment of the EWSR1::FLI1 Transcriptional Program in Ewing Sarcoma," by David McFadden as a supplement to #AACRpediatric26. buff.ly/useIazj
September 24, 2026 at 7:30 PM
Read this Cancer Discovery research article, "CDK8 Inhibition Induces Mediator Trapping and Impairment of the EWSR1::FLI1 Transcriptional Program in Ewing Sarcoma," by David McFadden as a supplement to #AACRpediatric26. buff.ly/useIazj
Researchers for the first time show that knocking out CDK8 & CDK19 in mice disrupts testosterone production and male fertility.
💬 They also share what it’s like trying to publish while navigating local funding pressures and global publishing norms.
buff.ly/jTfZdpl
💬 They also share what it’s like trying to publish while navigating local funding pressures and global publishing norms.
buff.ly/jTfZdpl
May 25, 2025 at 8:01 PM
Researchers for the first time show that knocking out CDK8 & CDK19 in mice disrupts testosterone production and male fertility.
💬 They also share what it’s like trying to publish while navigating local funding pressures and global publishing norms.
buff.ly/jTfZdpl
💬 They also share what it’s like trying to publish while navigating local funding pressures and global publishing norms.
buff.ly/jTfZdpl
CDK8 remodels the tumor microenvironment to resist the therapeutic efficacy of targeted KRASG12D inhibition in pancreatic ductal adenocarcinoma 🧪 www.biorxiv.org/content/10.1...
CDK8 remodels the tumor microenvironment to resist the therapeutic efficacy of targeted KRASG12D inhibition in pancreatic ductal adenocarcinoma
Mutations in KRAS are a dominant driver of pancreatic ductal adenocarcinoma (PDAC), with over 40% of PDAC patients presenting with KRASG12D mutations. The recent development of small molecule inhibito...
www.biorxiv.org
February 4, 2025 at 7:25 AM
CDK8 remodels the tumor microenvironment to resist the therapeutic efficacy of targeted KRASG12D inhibition in pancreatic ductal adenocarcinoma 🧪 www.biorxiv.org/content/10.1...
Low expression of OXCT1 promotes #TumorMigration & #ColorectalCancer liver #metastasis (#CRLM), while its upregulation suppresses the Wnt signaling pathway by downregulating CDK8 expression and histone H3 acetylation. #epigenetics #medsky
#OpenAccess: www.sciencedirect.com/science/arti...
#OpenAccess: www.sciencedirect.com/science/arti...
April 19, 2025 at 11:00 PM
Low expression of OXCT1 promotes #TumorMigration & #ColorectalCancer liver #metastasis (#CRLM), while its upregulation suppresses the Wnt signaling pathway by downregulating CDK8 expression and histone H3 acetylation. #epigenetics #medsky
#OpenAccess: www.sciencedirect.com/science/arti...
#OpenAccess: www.sciencedirect.com/science/arti...
I try yesterday with 4800 aa (med13l-med12l-cdk8) and it worked in less than 30min.
May 11, 2024 at 7:32 AM
I try yesterday with 4800 aa (med13l-med12l-cdk8) and it worked in less than 30min.
CDK8 coordinates jasmonate-induced immunity with sulfur-responsive defense in Arabidopsis
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
CDK8 coordinates jasmonate-induced immunity with sulfur-responsive defense in Arabidopsis | PNAS
Plant immunity draws heavily on carbon-, nitrogen-, and sulfur-based precursors for
the deployment of specialized defense compounds. The jasmonate ...
www.pnas.org
July 27, 2026 at 3:13 PM
CDK8 coordinates jasmonate-induced immunity with sulfur-responsive defense in Arabidopsis
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
INPUT CODE FOR ALBUM MUSIC ART:
corymahlermusic.bandcamp.com/yum
gnq4-c877
yw84-w2qv
2jzh-cdk8
a92j-wctn
8tx8-cc3j
hxg8-k3mw
hck3-y49s
hs3m-7qgl
44x9-vzrj
corymahlermusic.bandcamp.com/yum
gnq4-c877
yw84-w2qv
2jzh-cdk8
a92j-wctn
8tx8-cc3j
hxg8-k3mw
hck3-y49s
hs3m-7qgl
44x9-vzrj
Flalaski
corymahlermusic.bandcamp.com
April 27, 2026 at 5:41 PM
INPUT CODE FOR ALBUM MUSIC ART:
corymahlermusic.bandcamp.com/yum
gnq4-c877
yw84-w2qv
2jzh-cdk8
a92j-wctn
8tx8-cc3j
hxg8-k3mw
hck3-y49s
hs3m-7qgl
44x9-vzrj
corymahlermusic.bandcamp.com/yum
gnq4-c877
yw84-w2qv
2jzh-cdk8
a92j-wctn
8tx8-cc3j
hxg8-k3mw
hck3-y49s
hs3m-7qgl
44x9-vzrj
Scientists identify #OXCT1 as a #TumorSuppressor in #ColorectalLiverMetastasis, where its loss drives migration and #metastasis via CDK8–Wnt/β‑catenin signaling; low OXCT1 correlates with poor outcomes, highlighting therapeutic potential.
#OpenAccess: doi.org/10.1016/j.ge...
#OpenAccess: doi.org/10.1016/j.ge...
January 4, 2026 at 12:31 AM
Scientists identify #OXCT1 as a #TumorSuppressor in #ColorectalLiverMetastasis, where its loss drives migration and #metastasis via CDK8–Wnt/β‑catenin signaling; low OXCT1 correlates with poor outcomes, highlighting therapeutic potential.
#OpenAccess: doi.org/10.1016/j.ge...
#OpenAccess: doi.org/10.1016/j.ge...
And another PhD defence for our lab, this time, Katerina, the first PhD student of my main scientific partner-in-crime Sasha. Katerina is an absolutely fearless scientist who, despite being a chemist by training, can do everything from histology to bioinformatics.
June 24, 2025 at 5:43 PM
And another PhD defence for our lab, this time, Katerina, the first PhD student of my main scientific partner-in-crime Sasha. Katerina is an absolutely fearless scientist who, despite being a chemist by training, can do everything from histology to bioinformatics.
Targeting oncogenic KRAS in pancreatic cancer:
Raghu Kalluri and colleagues implicated tumor microenvironment remodeling by mediator kinase CDK8 in resistance development and as potential new vulnerability
link.springer.com/article/10.1...
Raghu Kalluri and colleagues implicated tumor microenvironment remodeling by mediator kinase CDK8 in resistance development and as potential new vulnerability
link.springer.com/article/10.1...
CDK8 remodels the tumor microenvironment and promotes resistance to KRASG12D inhibitors and daraxonrasib in PDAC - The EMBO Journal
Mutations in KRAS are a dominant driver of pancreatic ductal adenocarcinoma (PDAC), with about 50% of patients presenting with KRASG12D mutations. Small molecule inhibitors targeting KRASG12D suppress...
link.springer.com
July 14, 2026 at 12:57 PM
Targeting oncogenic KRAS in pancreatic cancer:
Raghu Kalluri and colleagues implicated tumor microenvironment remodeling by mediator kinase CDK8 in resistance development and as potential new vulnerability
link.springer.com/article/10.1...
Raghu Kalluri and colleagues implicated tumor microenvironment remodeling by mediator kinase CDK8 in resistance development and as potential new vulnerability
link.springer.com/article/10.1...
People with Down syndrome (DS) often experience heightened immune activity due to hyperactive interferon (IFN) signaling. Our latest research shows that inhibiting specific proteins, CDK8 and CDK19, could help dial down this overactive response. Read more: bit.ly/439vQ4E
February 27, 2025 at 10:16 PM
People with Down syndrome (DS) often experience heightened immune activity due to hyperactive interferon (IFN) signaling. Our latest research shows that inhibiting specific proteins, CDK8 and CDK19, could help dial down this overactive response. Read more: bit.ly/439vQ4E