METTL14 is crucial for limb development: disrupting it in mice affects 11 proteins, leading to abnormal cartilage and impaired chondrogenesis. PMID:40307229, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-59346-5 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development | Nature Communications
m6A RNA methylation is essential for many aspects of mammalian development but its roles in chondrogenesis remain largely unknown. Here, we show that m6A is necessary for chondrogenesis and limb morphogenesis using limb progenitor-specific knockout mice of Mettl14, an essential subunit in the m6A methyltransferase complex. The knockout disrupts cartilage anlagen formation in limb buds with 11 downregulated proteins known to dysregulate chondrogenesis and shorten limb skeletons upon mutation in mice and humans. Further studies show a gene regulatory hierarchy among the 11 proteins. m6A stabilizes the transcript and increases the protein level of GDF5, a BMP family member. This activates the chondrogenic transcription factor genes Runx2 and Runx3, whose mRNAs are also stabilized by m6A. They promote the transcription of six collagen genes and two other chondrogenic genes, Ddrgk1 and Pbxip1. Thus, this study uncovers an m6A-based cascade essential for chondrogenesis during limb skeletal d
doi.org
May 2, 2025 at 1:10 PM
In this work my team and co-workers demonstrated a purely mechanically regulated route for #StemCell #Chondrogenesis via engineering of the hydrogels #viscous nature. No #growthfactors needed. Conducted @uofglasgow.bsky.social @theglasgowcemi.bsky.social Funded @ukri.org
April 17, 2025 at 2:11 PM
In this work my team and co-workers demonstrated a purely mechanically regulated route for #StemCell #Chondrogenesis via engineering of the hydrogels #viscous nature. No #growthfactors needed. Conducted @uofglasgow.bsky.social @theglasgowcemi.bsky.social Funded @ukri.org
A low-annotation-budget PubMedBERT classifier for chondrogenesis regulator discovery via active learning https://www.biorxiv.org/content/10.64898/2026.09.24.754045v1
September 29, 2026 at 11:46 PM
A low-annotation-budget PubMedBERT classifier for chondrogenesis regulator discovery via active learning https://www.biorxiv.org/content/10.64898/2026.09.24.754045v1
A low-annotation-budget PubMedBERT classifier for chondrogenesis regulator discovery via active learning https://www.biorxiv.org/content/10.64898/2026.09.24.754045v1
September 29, 2026 at 11:46 PM
A low-annotation-budget PubMedBERT classifier for chondrogenesis regulator discovery via active learning https://www.biorxiv.org/content/10.64898/2026.09.24.754045v1
Can scaffolds heal joints? Study finds GCS/DBM scaffold enhances chondrogenesis for osteoarthritis. #Osteoarthritis #TissueEngineering
www.rimpacts.com/rd/p?c=10082... 📄DOI: doi.org/10.34172/bi....
www.rimpacts.com/rd/p?c=10082... 📄DOI: doi.org/10.34172/bi....
Chondrogenic Potential of Pmscs Cultured on Chondroitin Sulfate/ Gelatin-Modified Dbm Scaffold
Can scaffolds heal joints? Study finds GCS/DBM scaffold enhances chondrogenesis for osteoarthritis. #Osteoarthritis #TissueEngineering
www.rimpacts.com
December 16, 2025 at 5:54 AM
Can scaffolds heal joints? Study finds GCS/DBM scaffold enhances chondrogenesis for osteoarthritis. #Osteoarthritis #TissueEngineering
www.rimpacts.com/rd/p?c=10082... 📄DOI: doi.org/10.34172/bi....
www.rimpacts.com/rd/p?c=10082... 📄DOI: doi.org/10.34172/bi....
Thrilled to share our new bioRxiv preprint led by Eloise Fadial (@efadial.bsky.social) 🥳🎉! Using PRDM16 cKO mice, hiPSC models, scRNA-seq & CUT&RUN, Eloise shows that PRDM16 is a key genetic and epigenetic regulator of knee chondrogenesis and chondrocyte identity.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
www.biorxiv.org
February 7, 2026 at 4:52 PM
Thrilled to share our new bioRxiv preprint led by Eloise Fadial (@efadial.bsky.social) 🥳🎉! Using PRDM16 cKO mice, hiPSC models, scRNA-seq & CUT&RUN, Eloise shows that PRDM16 is a key genetic and epigenetic regulator of knee chondrogenesis and chondrocyte identity.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Mechanistically, she found that PRDM16 regulates knee chondrogenesis through DNA binding and by modulating H3K4me3 deposition at promoter and enhancer regions of key chondrogenic and hypertrophic genes (e.g., MEF2C), thereby shaping chondrocyte phenotype specification and joint homeostasis.
February 7, 2026 at 4:52 PM
Mechanistically, she found that PRDM16 regulates knee chondrogenesis through DNA binding and by modulating H3K4me3 deposition at promoter and enhancer regions of key chondrogenic and hypertrophic genes (e.g., MEF2C), thereby shaping chondrocyte phenotype specification and joint homeostasis.
Pro-longed (+1 wk) FGF9 treatment in Takasato #KidneyOrganoid
👉Delays ectopic #Chondrogenesis
👉⏫AQP2
👉Reduces mesenchymal compartment
👉Enhances vascularization
The performance when implanted?🧐
Vanessa LaPointe lab #NPJRegMed 2025
www.nature.com/articles/s41...
👉Delays ectopic #Chondrogenesis
👉⏫AQP2
👉Reduces mesenchymal compartment
👉Enhances vascularization
The performance when implanted?🧐
Vanessa LaPointe lab #NPJRegMed 2025
www.nature.com/articles/s41...
September 16, 2025 at 12:04 PM
Pro-longed (+1 wk) FGF9 treatment in Takasato #KidneyOrganoid
👉Delays ectopic #Chondrogenesis
👉⏫AQP2
👉Reduces mesenchymal compartment
👉Enhances vascularization
The performance when implanted?🧐
Vanessa LaPointe lab #NPJRegMed 2025
www.nature.com/articles/s41...
👉Delays ectopic #Chondrogenesis
👉⏫AQP2
👉Reduces mesenchymal compartment
👉Enhances vascularization
The performance when implanted?🧐
Vanessa LaPointe lab #NPJRegMed 2025
www.nature.com/articles/s41...
Twist1 is the first transcription factor differentially expressed in female cells. It is not expressed there either in mouse or chicken and has never been implicated in sex determination before! However, Twist1 has been shown to antagonize Sox9, a pro-testicular gene, but in mouse chondrogenesis 👀.
February 2, 2026 at 11:19 AM
Twist1 is the first transcription factor differentially expressed in female cells. It is not expressed there either in mouse or chicken and has never been implicated in sex determination before! However, Twist1 has been shown to antagonize Sox9, a pro-testicular gene, but in mouse chondrogenesis 👀.
#DBfeature 🐣
Influence of DNA-methylation at multiple stages of limb chondrogenesis
"A hypomethylated environment...change cell fate in the interdigital tissue, resulting in an ectopic digit"
by Jesús Chimal-Monroy, Jessica Marín-Llera et al
www.sciencedirect.com/science/arti...
Influence of DNA-methylation at multiple stages of limb chondrogenesis
"A hypomethylated environment...change cell fate in the interdigital tissue, resulting in an ectopic digit"
by Jesús Chimal-Monroy, Jessica Marín-Llera et al
www.sciencedirect.com/science/arti...
July 24, 2024 at 11:24 AM
#DBfeature 🐣
Influence of DNA-methylation at multiple stages of limb chondrogenesis
"A hypomethylated environment...change cell fate in the interdigital tissue, resulting in an ectopic digit"
by Jesús Chimal-Monroy, Jessica Marín-Llera et al
www.sciencedirect.com/science/arti...
Influence of DNA-methylation at multiple stages of limb chondrogenesis
"A hypomethylated environment...change cell fate in the interdigital tissue, resulting in an ectopic digit"
by Jesús Chimal-Monroy, Jessica Marín-Llera et al
www.sciencedirect.com/science/arti...
🚨Next DevBioConnect is live now
Dr. Diana Carlone is presenting CXXC Finger Protein 1 regulation of BMP signaling & Chondrogenesis during Limb development
www.sciencedirect.com/science/arti...
Join us on Zoom😆
utsouthwestern-edu.zoom.us/j/2815736063
Dr. Diana Carlone is presenting CXXC Finger Protein 1 regulation of BMP signaling & Chondrogenesis during Limb development
www.sciencedirect.com/science/arti...
Join us on Zoom😆
utsouthwestern-edu.zoom.us/j/2815736063
CXXC Finger Protein 1 drives BMP signaling and progenitor cell differentiation during limb development
The mechanisms mediating endochondral bone formation remain incompletely understood. Here, we show that CXXC Finger Protein 1 (CFP1) is required for t…
www.sciencedirect.com
March 10, 2026 at 4:09 PM
🚨Next DevBioConnect is live now
Dr. Diana Carlone is presenting CXXC Finger Protein 1 regulation of BMP signaling & Chondrogenesis during Limb development
www.sciencedirect.com/science/arti...
Join us on Zoom😆
utsouthwestern-edu.zoom.us/j/2815736063
Dr. Diana Carlone is presenting CXXC Finger Protein 1 regulation of BMP signaling & Chondrogenesis during Limb development
www.sciencedirect.com/science/arti...
Join us on Zoom😆
utsouthwestern-edu.zoom.us/j/2815736063
Our new paper is out! Well done Marc, Thais, Fran et al @cgm-newcastle.bsky.social! And thank you to @nc3rs.bsky.social
and the #JGWPattersonFoundation for funding and support.
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...
and the #JGWPattersonFoundation for funding and support.
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...
Dynamic Compression Improves Chondrogenesis in the Tissue Engineered Model of Cartilage
Hyaline cartilage is a dense avascular tissue with low regenerative potential, present at the ends of the diarthrodial joints and in the cartilage growth plate. Skeletal diseases often result from ex...
analyticalsciencejournals.onlinelibrary.wiley.com
May 27, 2025 at 12:53 PM
Our new paper is out! Well done Marc, Thais, Fran et al @cgm-newcastle.bsky.social! And thank you to @nc3rs.bsky.social
and the #JGWPattersonFoundation for funding and support.
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...
and the #JGWPattersonFoundation for funding and support.
analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...
A priming effect was seen when human bone-marrow stem cells were cultured on topographical substrates, enabling secreted factors to be captured, and then re-incorporated into the growth media of porcine chondrocytes to promote chondrogenesis and mediate inflammatory conditions. 🔬
October 1, 2025 at 10:49 AM
A priming effect was seen when human bone-marrow stem cells were cultured on topographical substrates, enabling secreted factors to be captured, and then re-incorporated into the growth media of porcine chondrocytes to promote chondrogenesis and mediate inflammatory conditions. 🔬
🚀New pre-print from the lab, led by PhD candidate Xiaolin Dong! www.biorxiv.org/content/10.6...
In this study, we uncovered crosstalk between mucin-type O-glycans and HSPGs, showing that truncated O-GalNAc glycans destabilize proteoglycans, impair FGF signaling, & disrupt chondrogenesis. #glycotime
In this study, we uncovered crosstalk between mucin-type O-glycans and HSPGs, showing that truncated O-GalNAc glycans destabilize proteoglycans, impair FGF signaling, & disrupt chondrogenesis. #glycotime
Mucin-type O-glycans regulate proteoglycan stability and chondrocyte maturation
O -glycosylation is a ubiquitous post-translational modification essential for protein stability, cell signaling, and tissue organization, yet how distinct O -glycan subclasses coordinate tissue devel...
www.biorxiv.org
December 18, 2025 at 3:08 AM
🚀New pre-print from the lab, led by PhD candidate Xiaolin Dong! www.biorxiv.org/content/10.6...
In this study, we uncovered crosstalk between mucin-type O-glycans and HSPGs, showing that truncated O-GalNAc glycans destabilize proteoglycans, impair FGF signaling, & disrupt chondrogenesis. #glycotime
In this study, we uncovered crosstalk between mucin-type O-glycans and HSPGs, showing that truncated O-GalNAc glycans destabilize proteoglycans, impair FGF signaling, & disrupt chondrogenesis. #glycotime
LAMA5 deficiency disrupts ECM-WNT crosstalk in chondrogenesis and contributes to idiopathic short stature https://www.biorxiv.org/content/10.1101/2025.11.19.689218v1
November 19, 2025 at 7:31 PM
LAMA5 deficiency disrupts ECM-WNT crosstalk in chondrogenesis and contributes to idiopathic short stature https://www.biorxiv.org/content/10.1101/2025.11.19.689218v1
A Vascularized Microphysiological System Reproducing Endochondral Ossification in Vitro to Study Ewing #Sarcoma Proliferation and Migration
advanced.onlinelibrary.wiley.com/doi/full/10....
advanced.onlinelibrary.wiley.com/doi/full/10....
A Vascularized Microphysiological System Reproducing Endochondral Ossification in Vitro to Study Ewing Sarcoma Proliferation and Migration
A biofabricated 3D in vitro model recapitulating endochondral ossification (ECO) is described, mimicking the steps from condensation to chondrogenesis and hypertrophy, culminating with vascularizatio...
advanced.onlinelibrary.wiley.com
September 28, 2025 at 1:24 PM
A Vascularized Microphysiological System Reproducing Endochondral Ossification in Vitro to Study Ewing #Sarcoma Proliferation and Migration
advanced.onlinelibrary.wiley.com/doi/full/10....
advanced.onlinelibrary.wiley.com/doi/full/10....
The authors included RRIDs in their in Bioactive Materials paper! RRIDs improve reproducibility in scientific research. #BetterScience #methodsmatter #RRID
Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
Read the full paper: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
doi.org
April 9, 2026 at 7:00 AM
The authors included RRIDs in their in Bioactive Materials paper! RRIDs improve reproducibility in scientific research. #BetterScience #methodsmatter #RRID
PRDM16 Coordinates Genetic and Epigenetic Programs Governing Chondrogenesis and Chondrocyte Phenotype Specification in the Knee Joint https://www.biorxiv.org/content/10.64898/2026.01.28.701140v1
February 1, 2026 at 5:00 AM
PRDM16 Coordinates Genetic and Epigenetic Programs Governing Chondrogenesis and Chondrocyte Phenotype Specification in the Knee Joint https://www.biorxiv.org/content/10.64898/2026.01.28.701140v1
#DBfeature #ICYMI
The epigenetic factor CFP1 regulates BMP signaling during limb development, expanding and maintaining mesenchymal progenitors and initiating chondrogenesis.
By Emanuele Pignatti, Lijie Jiang, Diana Carlone, et al.
tinyurl.com/y3xevhew
The epigenetic factor CFP1 regulates BMP signaling during limb development, expanding and maintaining mesenchymal progenitors and initiating chondrogenesis.
By Emanuele Pignatti, Lijie Jiang, Diana Carlone, et al.
tinyurl.com/y3xevhew
July 31, 2026 at 9:46 PM
#DBfeature #ICYMI
The epigenetic factor CFP1 regulates BMP signaling during limb development, expanding and maintaining mesenchymal progenitors and initiating chondrogenesis.
By Emanuele Pignatti, Lijie Jiang, Diana Carlone, et al.
tinyurl.com/y3xevhew
The epigenetic factor CFP1 regulates BMP signaling during limb development, expanding and maintaining mesenchymal progenitors and initiating chondrogenesis.
By Emanuele Pignatti, Lijie Jiang, Diana Carlone, et al.
tinyurl.com/y3xevhew
Haploinsufficiency of KPNA7 causes otosclerosis, likely due to the release of import inhibition of PTHrP and the reactivation of chondrogenesis in the globuli interossei https://www.biorxiv.org/content/10.1101/2025.08.22.671726v1
August 27, 2025 at 4:33 AM
Haploinsufficiency of KPNA7 causes otosclerosis, likely due to the release of import inhibition of PTHrP and the reactivation of chondrogenesis in the globuli interossei https://www.biorxiv.org/content/10.1101/2025.08.22.671726v1
I'll be presenting a poster from my PhD research at @limsltu.bsky.social and NUS Singapore highlighting the benefits of mesenchymal stem cell cultures on topographical substrates for cartilage regeneration (chondrogenesis).
October 1, 2025 at 10:49 AM
I'll be presenting a poster from my PhD research at @limsltu.bsky.social and NUS Singapore highlighting the benefits of mesenchymal stem cell cultures on topographical substrates for cartilage regeneration (chondrogenesis).
Excessive chondrogenesis in the blastema initiates during hypomorphic limb regeneration in Xenopus froglet, but not during patterned limb regeneration in Xenopus tadpoles and newts https://www.biorxiv.org/content/10.1101/2025.03.29.646070v1
April 2, 2025 at 7:30 PM
Excessive chondrogenesis in the blastema initiates during hypomorphic limb regeneration in Xenopus froglet, but not during patterned limb regeneration in Xenopus tadpoles and newts https://www.biorxiv.org/content/10.1101/2025.03.29.646070v1
Mutations in Fibronectin Dysregulate Chondrogenesis in Corner Fracture Type Spondylometaphyseal Dysplasia https://www.biorxiv.org/content/10.1101/2023.12.22.573039v1
Mutations in Fibronectin Dysregulate Chondrogenesis in Corner Fracture Type Spondylometaphyseal Dysplasia https://www.biorxiv.org/content/10.1101/2023.12.22.573039v1
Fibronectin (FN) is a key extracellular matrix glycoprotein indispensable for the development and fu
www.biorxiv.org
December 23, 2023 at 9:31 PM
Mutations in Fibronectin Dysregulate Chondrogenesis in Corner Fracture Type Spondylometaphyseal Dysplasia https://www.biorxiv.org/content/10.1101/2023.12.22.573039v1
RRIDs were included in this International Journal of Biological Macromolecules paper. Thanks for making your methods matter! #methodsmatter #OpenScience #RRID
Enhancing BMSC chondrogenesis with a dynamic viscoelastic hyaluronan hydrogel loaded with kartogenin for cartilage repair
doi.org
May 21, 2025 at 11:14 PM
RRIDs were included in this International Journal of Biological Macromolecules paper. Thanks for making your methods matter! #methodsmatter #OpenScience #RRID
The authors included RRIDs in their Nature Communications paper! RRIDs improve reproducibility in scientific research. #STMpublishing #ReproducibleResearch #reproducibility
METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development
doi.org
May 24, 2025 at 7:01 AM
The authors included RRIDs in their Nature Communications paper! RRIDs improve reproducibility in scientific research. #STMpublishing #ReproducibleResearch #reproducibility