#SmoothMuscleOnAChip with #CyclicStretch #Proteomics

PDMS #Microfluidic 1-mm-wide channel + Fluigent Push Pull pump

MOVAS SMC in collagen #Hydrogel

static vs 7% physiologic vs 19% pathologic strain

Mechanic stress-induced #ERStress

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#TemporoMandibularJoint #OsteoArthritis

#FGF9/FGFR3 acts downstream of ⬆️VWA1 in Compensatory responses (⏫ColII ACAN BCL2) of Condylar Cartilage in rat +0.5-mm Zr occlusal plate on molars
& Chondrocyte +6% #CyclicStretch

#ArthritisRes 2025
arthritis-research.biomedcentral.com/articles/10....

#Piezo1 #YAP Bioenergetics

Human Umbilical Cord MSC

#CyclicStretch (10% strain, 0.5Hz, 7 d)
#Piezo➡️
YAP nuclear import➡️
⏫MFN1/2 ⏬DRP1➡️
⏫Mitochondrial fusion ⏫mtDNA-encoded genes➡️
⏫OXPHOS (⏫Glycolysis)➡️
⏫SMC contractile proteins

#FASEBJ 2025
faseb.onlinelibrary.wiley.com/doi/10.1096/...

Mechanical Control of Inflammation

#CyclicStretch (10% strain, 1 Hz, 6 h) ⏬TNFα-induced Inflammation of 👤Aortic Smooth Muscle Cell on collagen I
Indie of NF-κB?🥸

Bulk RNAseq➡️
15% (pathological) vs 10% vs static on collagen vs fibronectin vs laminin

bioRxiv 2025
www.biorxiv.org/content/10.1...

#YAP #VeinGraftDisease

#CyclicStretch (10% strain 1 Hz), or TGFβ1+TSP-1▶️
⏫YAP nuclear enrichment
⏫migration/proliferation/collagen
in👤CD34+CD31- Saphenous Vein progenitors

Verteporfin, YAP/TAZi
⏬intimal hyperplasia
in ApoE*3-Leiden🐭vein graft

#JAHA_AHA 2025
www.ahajournals.org/doi/10.1161/...

#FGF9 ➡️Mechanosensitive Osteocyte-to-Osteoblast/Osteoclast signaling in Maxillary Bone formation

#CyclicStretch ➡️⏬FGF9 in MC3T3 preosteoblasts (😆)

FGF9⏫ in underdeveloped🧍‍♂️Maxilla

FGF9 ⏬osteogenesis &⏫osteoclastogenesis
ATF4/5/6 NR2F1

#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...

#SmoothMuscleCell #Aging Mechanobiology

24- vs 4-mo rat SkM feed artery

Aged SMC
👉RNAseq ⏬Contractile gene #FocalAdhesion
👉Faster kinetics⏫αSMA filament ⏫vinculin cluster to #CyclicStretch
👉Pressure Inert on 4 kPa matrix; Sensitive on 100 kPa

#FrontPhysiol 2025
www.frontiersin.org/journals/phy...

🔬 New preprint !!! 👇
📃 shorturl.at/uTj8m
🧫 We identified NET1/ARHGEF8 as a stretch-sensitive RhoGEF in smooth muscle cells
Thanks to Mary-Adel Mrad and the team for their hard work 💪
#vascularbiology #smoothmusclecell #cyclicstretch↔️
@inserm.fr @cnrsbiologie.bsky.social