#SmoothMuscleCell
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haoyin.bsky.social
Epigenetic regulation of vascular #SmoothMuscleCell plasticity in vascular injury, repair & aging

PRMT5, EZH2, LSD1, CBP/P300, heterochromatin, BRD4

Happy to contribute a bit (Mechano-epigenetics; Aneurysm) to this review by my PhD supervisor😁

#VasPharma 2026
www.sciencedirect.com/science/arti...
October 25, 2026 at 7:49 PM
haoyin.bsky.social
Human aorta is a mm-thick 50-layered organ

#SmoothMuscleCell phenotypic gradients across aortic media?👇

Here is an important study from Dr. Hiromi Yanagisawa

Micro-biopsies RNAseq from 4 distinct layers in 👤aortic media

5 BAV + 4 Sporadic

#AortaEd
#JVSVS 2026
jvsvs.org/article/S266...
October 23, 2026 at 6:54 PM
haoyin.bsky.social
A high-density micromass (50k cells/10 µL) #SmoothMuscleCell calcification assay

Mimicking atherosclerotic chondromyocyte state (100-gene marker panel)
Or mesenchymal condensation?

240/552 #CoronaryArteryDisease risk genes are differentially expressed

bioRxiv 2026
www.biorxiv.org/content/10.6...
October 3, 2026 at 1:23 PM
haoyin.bsky.social
Carbonic anhydrase 9, an intracellular pH regulator, is widely used to mark #Hypoxia in tumors (+some tissues)

Here's new evidence for CA9 (+CA12) in human pulmonary arterial #SmoothMuscleCell & 🐭#PulmonaryHypertension (S4, CA9/CA12 dual inhibitor) 😎

#AJRCMB 2026
academic.oup.com/ajrcmb/advan...
October 1, 2026 at 11:32 AM
haoyin.bsky.social
#SpatialTranscriptomics #Atherosclerosis

CosMx 6k @brukerspatial.bsky.social over 1119 FOVs, 4 patients (♀️♂️ stable/unstable plaque)➡️
Sex-/Stability-related lesional cell architecture😎

+scRNAseq
#SmoothMuscleCell, T, Mφ in sex-related plaque stability

#ATVB 2026
www.ahajournals.org/doi/10.1161/...
May 23, 2026 at 12:18 PM
haoyin.bsky.social
#Ferroptosis #VascularCalcification

SREBP1 transcriptionally⏫iASPP (likely FASN▶️FAO, BHLHE40▶️Circadian, too)➡️ ⏬#SmoothMuscleCell #Ferroptosis #Osteogenesis (BMP2 RUNX2 Calcification)

#CardiovascDiabetol 2025
cardiab.biomedcentral.com/articles/10....
May 3, 2026 at 7:01 PM
haoyin.bsky.social
NAMPT #AbdominalAorticAneurysm

👤AAA
⏬NAMPT OXPHOS mitoETC #SmoothMuscleCell contractile phenotype
⏫Senescence
⏫B cell pDC

1⃣#ExtracellularVesicle from NAMPT-OE #hiPSC-#MesenchymalStemCell or 2⃣P7C3 NAMPTa
⬆️SMC NAD+
⬇️SMC senescence 🐭AAA

#BioactiveMat 2023
www.sciencedirect.com/science/arti...
April 30, 2026 at 6:47 PM
haoyin.bsky.social
miR-26b as a repressor #AorticMedialCalcification

⏬Aortic Fibroblast BMP4 to #SmoothMuscleCell SMAD1 (direct target gene of miR-26) signaling

miR-26b KO➡️spontaneous aortic calcification
+ #EndothelialCell SMC Fibroblast states scRNAseq

#CardiovascRes 2025
academic.oup.com/cardiovascre...
April 28, 2026 at 12:01 PM
haoyin.bsky.social
Loss of contractile phenotype (⏬SMMHC SMTN ⏫S100A4 MMP2) of #SmoothMuscleCell & Elastin fiber in #AcuteAorticDissection

Type A/B? Any aneurysm?🧐

24 case (non-monogenic) vs 17 control

"aortic wall adjacent to the entry site of the dissection"

#AortaEd
#CJCOpen 2025
www.cjcopen.ca/article/S258...
March 15, 2026 at 7:26 PM
haoyin.bsky.social
#SIRT6 #VascularCalcification

Rat #HighFatDiet + VitD3 + Nicotine
▶️Aortic medial calcification & ⏬SIRT6

SIRT6 overexpression ⏬Palmitic acid-induced #SmoothMuscleCell Apoptosis & Osteogenesis

#JHyperten 2023
journals.lww.com/jhypertensio...
March 8, 2026 at 7:07 PM
haoyin.bsky.social
#Autophagy #VascularAging

SM22α+ #SmoothMuscleCell Atg7 KO🐭3.5 mo
Aorta myography with oscillatory tension 10 Hz

👉Peterson modulus⏫
pressure-dependent
systolic 80~300 mmHg
diastolic 40~260 mmHg

↔️🫀functions

Paul Fransen lab #PflügersArchiv 2020
link.springer.com/article/10.1...
February 27, 2026 at 1:00 PM
haoyin.bsky.social
Transcript signature of lineage-specific mouse proximal thoracic aortic #SmoothMuscleCell

Wnt1-Cre cardiac neural crest (inner media)-derived SMC
vs
Mef2c-Cre second heart field (outer media)-derived SMC

#AortaEd
David Dichek lab #ATVB 2025
www.ahajournals.org/doi/10.1161/...
February 22, 2026 at 8:18 PM
haoyin.bsky.social
1400W, an iNOS inhibitor, reduces #ThoracicAorticAneurysm #ThroacicAorticDissecion in BAPN mouse, in addition to previously reported Marfan mice

A possible role in #SmoothMuscleCell Mitochondrial fission/fusion

#AortaEd

#EurJPharm 2025
www.sciencedirect.com/science/arti...
February 11, 2026 at 8:51 PM
haoyin.bsky.social
#VascularCalcification

🐭aortic #SmoothMuscleCell in anchored 3D fibrin hydrogel for 14 d
👉Accumulate proteoglycan (IHC Proteomics)
👉No elastin fiber formation
👉TGFβ-responsive
👉Calcifiable, retaining similar individual sensitivity to 2D culture

www.sciencedirect.com/science/arti...
February 3, 2026 at 12:59 PM
haoyin.bsky.social
#SmoothMuscleCell #Aging Mechanobiology

24- vs 4-mo rat SkM feed artery

Aged SMC
👉RNAseq ⏬Contractile gene #FocalAdhesion
👉Faster kinetics⏫αSMA filament ⏫vinculin cluster to #CyclicStretch
👉Pressure Inert on 4 kPa matrix; Sensitive on 100 kPa

#FrontPhysiol 2025
www.frontiersin.org/journals/phy...
January 5, 2026 at 8:36 PM
haoyin.bsky.social
#VeinAging
Great saphenous vein 18~89 y/o

Normal
👉Subintimal longitudinal #SmoothMuscleCell bundle peak at middle age
👉Outer medial longitudinal SMC bundle ⬆️with Age

Varicose
👉Alternating zones of hypertrophic & atrophic SMCs
👉Excess collagen deposit

#JCM_MDPI 2025
www.mdpi.com/2077-0383/14...
December 30, 2025 at 8:15 PM
haoyin.bsky.social
Contribution of Gli1+ #AdventitialStemCell to #SmoothMuscleCell
❌Atherosclerosis
❌Wire-Injured Femoral Artery
✅Femoral Artery Anastomosis

1⃣Gli1-CreER;R26-tdT🐭
2⃣Myh11-Dre;Gli1-CreER;R26-IR1🐭
Gli1+ lineage➡️ZsG
Preexisting SMC➡️tdT

Adv Sci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
December 8, 2025 at 12:49 PM
haoyin.bsky.social
#HutchinsonGilfordProgeriaSyndrome

#SmoothMuscleCell Progerin transgene drives #EndMT #Atherosclerosis🐭

⏫Aortic Endothelial Cell permeability
⏫Lesional EC infiltr▶️ColIV- & TGFβ1-rich plaque😮

Paracrine of progeroid aortic SMC?😆

#CircAHA 2024
www.ahajournals.org/doi/10.1161/...
December 4, 2025 at 11:55 AM
haoyin.bsky.social
The quantitative analysis of Re-orientation of #SmoothMuscleCell #CollagenFiber in🐭#Atherosclerosis

#Calcification Annexin A2/5/6 (#MatrixVesicle markers)⬆️ only seen in zones of SMC/Collagen disorganization

Xufeng Niu & Yubo Fan labs Research 2025
spj.science.org/doi/10.34133...
November 30, 2025 at 8:03 PM
haoyin.bsky.social
Gelsolin as a selective #Pericyte marker vs #SmoothMuscleCell Fibroblast in mouse #SkeletalMuscle
Antibody R&D MAB8170 Cryosection

BulkRNAseq
(SkM PDGFRβ+SMA− pericyte vs PDGFRβ+SMA+ SMC) vs (Brain pericyte vs SMC)

#SciRep 2025
www.nature.com/articles/s41...
November 26, 2025 at 8:53 PM
haoyin.bsky.social
PRMT5 di-methylates H3R8 & H4R3▶️
⏬Myocardin-dependent #SmoothMuscleCell contractile gene transcription
⏫SMC proliferation/migration & intimal hyperplasia in injured🐭carotid artery

PRMT5 (& PRMT4) ⬆️in PDGBB-treated SMC &👤coronary #Atherosclerosis

#CardiovascRes 2023
academic.oup.com/cardiovascre...
November 22, 2025 at 8:22 PM
haoyin.bsky.social
PRMT5 methylates & stabilizes KLF4 protein▶️
⏫#SmoothMuscleCell proliferation/migration & intimal hyperplasia in injured🐭carotid artery

Evidence from #SmoothMuscleCell PRMT5 KO🐭 & Lentiviral PRMT5

PRMT5⬆️ in👤carotid stenosis & PDGF-BB-treated SMC

#CardioVascRes 2023
academic.oup.com/cardiovascre...
November 20, 2025 at 8:10 PM
haoyin.bsky.social
PRMT5 methylates NF-κB p65 to enhance trimethylamine N-oxide-NOX4-induced #SmoothMuscleCell inflammation

SM22α+ SMC PRMT5 KO🐭 + carotid wire injury + TMAO i.p.▶️
⏬p65 methylation
⏬Vascular cell VCAM1
⏬CD68 recruitment

#CellDeathDis 2022
www.nature.com/articles/s41...
November 17, 2025 at 8:04 PM
haoyin.bsky.social
#SmoothMuscleCell m6A Epitranscriptomics

METTL14 ⬆️in 🐭 #ThoracicAorticAneurysm (AngII+HFD) or erastin-treated SMC

Liproxstatin-1 or AAV-shMettl14⏬🐭TAA

METTL14-IGF2BP2 stabilizes ACSL4 mRNA➡️
SMC #Ferroptosis

SRAMP➡️m6A site prediction

#AJPCellPhys 2025
journals.physiology.org/doi/full/10....
November 16, 2025 at 8:34 PM
haoyin.bsky.social
#SmoothMuscleCell Mettl14, ⬆️in injured artery, aggravates #IntimalHyperplasia via ⏫nascent KLF4 & SERPINE1 mRNA synthesis (Not impacting mRNA stability)

Likely Mettl3, but not other m6A readers/writers/erasers, plays a minor role

@insight.jci.org 2025
insight.jci.org/articles/vie...
November 13, 2025 at 8:02 PM