🔬🐦 Zebra finch retina!
AAV-mediated GCaMP expression reveals elongated photoreceptors
🟢 GCaMP | 🔴 DAPI | ⚪ Transmission: distinctive oil droplets in the photoreceptor layer
These droplets contribute to spectral filtering
#FluorescenceFriday #ZebraFinch #Retina #Vision #GCaMP #Neuroscience
AAV-mediated GCaMP expression reveals elongated photoreceptors
🟢 GCaMP | 🔴 DAPI | ⚪ Transmission: distinctive oil droplets in the photoreceptor layer
These droplets contribute to spectral filtering
#FluorescenceFriday #ZebraFinch #Retina #Vision #GCaMP #Neuroscience
September 25, 2026 at 10:53 AM
🔬🐦 Zebra finch retina!
AAV-mediated GCaMP expression reveals elongated photoreceptors
🟢 GCaMP | 🔴 DAPI | ⚪ Transmission: distinctive oil droplets in the photoreceptor layer
These droplets contribute to spectral filtering
#FluorescenceFriday #ZebraFinch #Retina #Vision #GCaMP #Neuroscience
AAV-mediated GCaMP expression reveals elongated photoreceptors
🟢 GCaMP | 🔴 DAPI | ⚪ Transmission: distinctive oil droplets in the photoreceptor layer
These droplets contribute to spectral filtering
#FluorescenceFriday #ZebraFinch #Retina #Vision #GCaMP #Neuroscience
October 31, 2025 at 2:09 PM
a GCaMP tadpole for #fluorescenceFriday as an introductory hello on BlueSky :) Transgenic by Lora Sweeney (ISTA), and picci by our postdoc Michi Forsthofer
November 16, 2024 at 2:01 PM
a GCaMP tadpole for #fluorescenceFriday as an introductory hello on BlueSky :) Transgenic by Lora Sweeney (ISTA), and picci by our postdoc Michi Forsthofer
Resolving synaptic events using subsynaptically targeted GCaMP8 variants by Dion K Dickman and team: www.biorxiv.org/content/10.1...
June 20, 2025 at 9:30 PM
Resolving synaptic events using subsynaptically targeted GCaMP8 variants by Dion K Dickman and team: www.biorxiv.org/content/10.1...
Here is a cool example of just how sensitive these skin cells are to touch using GCaMP calcium imaging in a living mouse!
December 11, 2024 at 9:23 PM
Here is a cool example of just how sensitive these skin cells are to touch using GCaMP calcium imaging in a living mouse!
Pairing this assay with our cichlid GCaMP calcium imaging will let us watch neural activity while they perform the behavior—connecting sensory processing, motor control, and evolutionary change in a highly accessible vertebrate system. Looking forward to more #neuroethology in this model!
September 27, 2026 at 3:03 PM
Pairing this assay with our cichlid GCaMP calcium imaging will let us watch neural activity while they perform the behavior—connecting sensory processing, motor control, and evolutionary change in a highly accessible vertebrate system. Looking forward to more #neuroethology in this model!
Posting a second #FluorescenceFriday of images just off the scope of cre-dependent GCaMP in locus coeruleus neurons. Huge thanks to @lindsayschwarz.bsky.social for helping us out with this!!
November 15, 2024 at 10:28 PM
Posting a second #FluorescenceFriday of images just off the scope of cre-dependent GCaMP in locus coeruleus neurons. Huge thanks to @lindsayschwarz.bsky.social for helping us out with this!!
2 beautiful cells expressing #GCaMP in #chicken retina via AAV-mediated retinal #transduction.
The dendritic fields of these cells are vast, beautiful and ordered, depicting how truly intricate #retina connectivity is. #Avian retina is remarkable.
My choice for today's #FluorescenceFriday image.
The dendritic fields of these cells are vast, beautiful and ordered, depicting how truly intricate #retina connectivity is. #Avian retina is remarkable.
My choice for today's #FluorescenceFriday image.
March 13, 2025 at 10:12 PM
2 beautiful cells expressing #GCaMP in #chicken retina via AAV-mediated retinal #transduction.
The dendritic fields of these cells are vast, beautiful and ordered, depicting how truly intricate #retina connectivity is. #Avian retina is remarkable.
My choice for today's #FluorescenceFriday image.
The dendritic fields of these cells are vast, beautiful and ordered, depicting how truly intricate #retina connectivity is. #Avian retina is remarkable.
My choice for today's #FluorescenceFriday image.
With everything that's going on, I needed this cool data 1 day before #flourescencefriday. It's my first time with #GCaMP imaging (green), and you can clearly see Aβ42 induced hyperexcitability at the same time that we also see BBB leakage (not shown)! #zebrafishrock
February 28, 2025 at 1:32 AM
With everything that's going on, I needed this cool data 1 day before #flourescencefriday. It's my first time with #GCaMP imaging (green), and you can clearly see Aβ42 induced hyperexcitability at the same time that we also see BBB leakage (not shown)! #zebrafishrock
Completely agree! E.g: In this preprint, I co-expressed axon-GCaMP and inhibitory DREADD (H4) and showed that local CNO infusion attenuates terminal Ca2+ during singing. In my case, I wanted to validate the DREADD, but this can also be used the other way around (1/3)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
January 28, 2026 at 5:21 PM
Completely agree! E.g: In this preprint, I co-expressed axon-GCaMP and inhibitory DREADD (H4) and showed that local CNO infusion attenuates terminal Ca2+ during singing. In my case, I wanted to validate the DREADD, but this can also be used the other way around (1/3)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
January 18, 2025 at 6:22 PM
Anybody else seeing SCN Per2Luc/GCaMP rhythms? @jonesjeffr.bsky.social @erikherzog.bsky.social
Unveil the allure of Welo Fire Opals from Ethiopia, known for their stunning color play and unique hydrophanic properties. A gem that transforms with light and water.
May 31, 2026 at 10:06 AM
Anybody else seeing SCN Per2Luc/GCaMP rhythms? @jonesjeffr.bsky.social @erikherzog.bsky.social
It's been a year in the making but I am happy to announce our UNC Neurophysiology core facility is online. What do we do? Patch clamp (from almost anything), calcium imaging, ABR, EEG, and more! Ready to help labs, research institutes, and companies anywhere! Details: www.med.unc.edu/neurophyscore/
July 17, 2026 at 6:46 PM
It's been a year in the making but I am happy to announce our UNC Neurophysiology core facility is online. What do we do? Patch clamp (from almost anything), calcium imaging, ABR, EEG, and more! Ready to help labs, research institutes, and companies anywhere! Details: www.med.unc.edu/neurophyscore/
How we did it: we register 4 views together, in-vivo 2p GCaMP functional, in-vivo GCaMP z-stack, ex-vivo GCaMP confocal and finally MERSCOPE imaging from @vizgen_inc.
Below is a snapshot of the interactive website where you can freely inspect the 4-way alignment.
Below is a snapshot of the interactive website where you can freely inspect the 4-way alignment.
April 10, 2026 at 1:42 AM
How we did it: we register 4 views together, in-vivo 2p GCaMP functional, in-vivo GCaMP z-stack, ex-vivo GCaMP confocal and finally MERSCOPE imaging from @vizgen_inc.
Below is a snapshot of the interactive website where you can freely inspect the 4-way alignment.
Below is a snapshot of the interactive website where you can freely inspect the 4-way alignment.
fluorescence lifetime of GCaMP, i.e. how long it takes GCaMP to emit a photon after excitation. In this paper, they make a GCaMP6s variant that is ~3x faster than the original using a fluorescent protein called BrUSLEE. This makes sense because Bruce Lee was ~3x faster than a normal human 3/
November 22, 2024 at 5:17 PM
fluorescence lifetime of GCaMP, i.e. how long it takes GCaMP to emit a photon after excitation. In this paper, they make a GCaMP6s variant that is ~3x faster than the original using a fluorescent protein called BrUSLEE. This makes sense because Bruce Lee was ~3x faster than a normal human 3/
#FluorescentFriday The lab’s 1st slice physiology recordings! Timelapse recording of striatal neurons ex vivo using a Thy1-GCaMP mouse 🐭 🧠 + glutamate in the bath. Thanks @neuromanonyc.bsky.social! Shout out to @keyenceusa.bsky.social who has been great to work with customizing the BZ-X800🔬
September 13, 2025 at 1:59 AM
#FluorescentFriday The lab’s 1st slice physiology recordings! Timelapse recording of striatal neurons ex vivo using a Thy1-GCaMP mouse 🐭 🧠 + glutamate in the bath. Thanks @neuromanonyc.bsky.social! Shout out to @keyenceusa.bsky.social who has been great to work with customizing the BZ-X800🔬
Huge thanks to Benj Plackett & the @nature.com journalism team for featuring our tardigrade 🧠🔬🧬 work! Means so much as an early-career researcher building community + tools (neural atlas, GCaMP strain, behavior) now based in @gaou-ak.bsky.social Lab @ IAB Tsuruoka 🇯🇵 www.nature.com/articles/d41...
Tardigrade-obsessed: meet the researchers trying to turn water bears into neuroscience models
For water-bear enthusiasts, it’s the creature’s legs that make it stand out.
www.nature.com
June 24, 2025 at 2:16 AM
Huge thanks to Benj Plackett & the @nature.com journalism team for featuring our tardigrade 🧠🔬🧬 work! Means so much as an early-career researcher building community + tools (neural atlas, GCaMP strain, behavior) now based in @gaou-ak.bsky.social Lab @ IAB Tsuruoka 🇯🇵 www.nature.com/articles/d41...
Check out this compact 920 nm ultrafast fs laser for 2p imaging of GCaMP, etc.. Mounted on its side like this, it takes up very little space on the table.
That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.
That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.
July 27, 2026 at 7:39 PM
Check out this compact 920 nm ultrafast fs laser for 2p imaging of GCaMP, etc.. Mounted on its side like this, it takes up very little space on the table.
That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.
That's the Axon 920, generously loaned to the course thanks to @ewadorotaz.bsky.social and others at Coherent. It's what we use on our own rigs.
A basic image for #fluorescencefriday -- neurons expressing the Ca++ sensor, GCaMP, imaged with a 2-photon microscope. The cool thing is the microscope is built by neuroscience students on our PhD optics course. It is the last thing they build at the end of their two week optics journey.
November 22, 2024 at 11:12 AM
A basic image for #fluorescencefriday -- neurons expressing the Ca++ sensor, GCaMP, imaged with a 2-photon microscope. The cool thing is the microscope is built by neuroscience students on our PhD optics course. It is the last thing they build at the end of their two week optics journey.
The 'sparks' of life! Our latest work on the role of #calcium dynamics in the early #embryo. 🎇🔬🐁
@idrm.ox.ac.uk @oxforddpag.bsky.social @srinivas-lab.bsky.social #lightsheetmicroscopy #GCAMP
@idrm.ox.ac.uk @oxforddpag.bsky.social @srinivas-lab.bsky.social #lightsheetmicroscopy #GCAMP
During #development, distal visceral #endoderm (DVE) cells migrate to shape A-P axis specification. @m-stower.bsky.social @srinivas-lab.bsky.social &co reveal the role of Ca2+ dynamics in this, showing that inhibition of #calcium transients causes DVE arrest @plosbiology.org 🧪 plos.io/4nHWxF4
October 8, 2025 at 2:56 PM
The 'sparks' of life! Our latest work on the role of #calcium dynamics in the early #embryo. 🎇🔬🐁
@idrm.ox.ac.uk @oxforddpag.bsky.social @srinivas-lab.bsky.social #lightsheetmicroscopy #GCAMP
@idrm.ox.ac.uk @oxforddpag.bsky.social @srinivas-lab.bsky.social #lightsheetmicroscopy #GCAMP
Suppl. Video 5. Prefrontal cortex (red: alb-mScarlet-SpyTag003, green: neuronal GCaMP; kudos to @thomasbienvenu.bsky.social )
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
March 25, 2025 at 2:08 PM
Suppl. Video 5. Prefrontal cortex (red: alb-mScarlet-SpyTag003, green: neuronal GCaMP; kudos to @thomasbienvenu.bsky.social )
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
#FluorescenceFriday rehash. DRG cultures expressing ER-targeted GCaMP and treated with CMXRos. May be “old” data, but finding it tonight instantly reminded me of how much I love these organelles.
December 21, 2024 at 2:57 AM
#FluorescenceFriday rehash. DRG cultures expressing ER-targeted GCaMP and treated with CMXRos. May be “old” data, but finding it tonight instantly reminded me of how much I love these organelles.
To test for crosstalk, we compared activity in overlapping GCaMP and dTomato signals. Calcium events in the GCaMP channel showed no corresponding change in dTomato, confirming minimal crosstalk between channels 🎯
(5/10)
(5/10)
July 14, 2025 at 4:55 PM
To test for crosstalk, we compared activity in overlapping GCaMP and dTomato signals. Calcium events in the GCaMP channel showed no corresponding change in dTomato, confirming minimal crosstalk between channels 🎯
(5/10)
(5/10)
Not sure if our 'striking image' we submitted will get used but here it is. Super striking, if you ask me!
Credit - @mathiasgua.bsky.social
Credit - @mathiasgua.bsky.social
February 5, 2025 at 4:28 PM
Not sure if our 'striking image' we submitted will get used but here it is. Super striking, if you ask me!
Credit - @mathiasgua.bsky.social
Credit - @mathiasgua.bsky.social