Linking the glycome and transcriptome at the single cell level #glycotime
Online now! Online now! Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies #chembiol
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
Marglous et al. generated a database of anti-glycan antibody sequences, expressed and characterized a library of 154 antibodies, and generated DNA-barcoded antibodies for single-cell transcriptome and glycome profiling. Glycans associated with cancer stem cells and metastasis were uncovered, demonstrating utility for basic and clinical research.
dlvr.it
December 15, 2025 at 4:54 PM
Linking the glycome and transcriptome at the single cell level #glycotime
(Whispers “look at the glycome”)
March 20, 2025 at 5:08 AM
(Whispers “look at the glycome”)
We were hesitant about such an approach, due to the potential loss of glycome specificity resulting from the mixed glycomes, but surprisingly, we see higher glycan intensities AND the same structures are specifically attributed to the correct glycome.
October 1, 2026 at 2:12 PM
We were hesitant about such an approach, due to the potential loss of glycome specificity resulting from the mixed glycomes, but surprisingly, we see higher glycan intensities AND the same structures are specifically attributed to the correct glycome.
MALDI imaging of lipids, metabolites and glycans all on the same section! From Ramon Sun and colleagues. www.nature.com/articles/s41...
Spatial mapping of the brain metabolome lipidome and glycome - Nature Communications
Clarke et al. presents a framework for spatial analysis of the metabolome, lipidome, and glycome from a single tissue section using mass spectrometry imaging. Applying this approach, they revealed reg...
www.nature.com
May 12, 2025 at 7:34 PM
MALDI imaging of lipids, metabolites and glycans all on the same section! From Ramon Sun and colleagues. www.nature.com/articles/s41...
It was great to attend #HUPO2026 and catch up with new friends and old.
Here's our (@maiakelly.bsky.social) main poster for the conference, achieving the most comprehensive representation of the glycome to date with N-, O-, GSL-, and free- glycans acquired in a single LC-MS run. #glycotime
Here's our (@maiakelly.bsky.social) main poster for the conference, achieving the most comprehensive representation of the glycome to date with N-, O-, GSL-, and free- glycans acquired in a single LC-MS run. #glycotime
October 1, 2026 at 2:10 PM
It was great to attend #HUPO2026 and catch up with new friends and old.
Here's our (@maiakelly.bsky.social) main poster for the conference, achieving the most comprehensive representation of the glycome to date with N-, O-, GSL-, and free- glycans acquired in a single LC-MS run. #glycotime
Here's our (@maiakelly.bsky.social) main poster for the conference, achieving the most comprehensive representation of the glycome to date with N-, O-, GSL-, and free- glycans acquired in a single LC-MS run. #glycotime
Online now! Online now! Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies #chembiol
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
Marglous et al. generated a database of anti-glycan antibody sequences, expressed and characterized a library of 154 antibodies, and generated DNA-barcoded antibodies for single-cell transcriptome and glycome profiling. Glycans associated with cancer stem cells and metastasis were uncovered, demonstrating utility for basic and clinical research.
dlvr.it
December 15, 2025 at 4:30 PM
Online now! Online now! Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies #chembiol
March 30, 2026 at 10:39 AM
We've just published a new scientific preprint that dives into the spatial glycome using super-resolution microscopy.
Discover the critical role of sugars and take a look at our visualizations revealing their cell-wide presence. -> www.biorxiv.org/content/10.1...
#dSTORM #SMLM #glycobiology
Discover the critical role of sugars and take a look at our visualizations revealing their cell-wide presence. -> www.biorxiv.org/content/10.1...
#dSTORM #SMLM #glycobiology
February 25, 2025 at 10:36 AM
We've just published a new scientific preprint that dives into the spatial glycome using super-resolution microscopy.
Discover the critical role of sugars and take a look at our visualizations revealing their cell-wide presence. -> www.biorxiv.org/content/10.1...
#dSTORM #SMLM #glycobiology
Discover the critical role of sugars and take a look at our visualizations revealing their cell-wide presence. -> www.biorxiv.org/content/10.1...
#dSTORM #SMLM #glycobiology
Closing out the day at #Glyco27 with Dr. Kenji Kadomatsu from @en.nagoya-u.ac.jp talking about glyconeuroscience and the Human Glycome Atlas Project!
#glycotime #glycomics #glycobiology
#glycotime #glycomics #glycobiology
May 28, 2025 at 12:52 AM
Closing out the day at #Glyco27 with Dr. Kenji Kadomatsu from @en.nagoya-u.ac.jp talking about glyconeuroscience and the Human Glycome Atlas Project!
#glycotime #glycomics #glycobiology
#glycotime #glycomics #glycobiology
Assignment complete 🫡 😀
"the structural diversity and potential chemical information of the glycome far exceeds that of the genome or proteome"- Lloyd, 2021
"the structural diversity and potential chemical information of the glycome far exceeds that of the genome or proteome"- Lloyd, 2021
November 19, 2024 at 6:45 AM
Assignment complete 🫡 😀
"the structural diversity and potential chemical information of the glycome far exceeds that of the genome or proteome"- Lloyd, 2021
"the structural diversity and potential chemical information of the glycome far exceeds that of the genome or proteome"- Lloyd, 2021
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
www.cell.com/cell-chemica...
@cp-cellchembiol.bsky.social
www.cell.com/cell-chemica...
@cp-cellchembiol.bsky.social
December 11, 2025 at 2:29 AM
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
www.cell.com/cell-chemica...
@cp-cellchembiol.bsky.social
www.cell.com/cell-chemica...
@cp-cellchembiol.bsky.social
Too cool to be true? 😎 Nope! GlycoShape ReGlyco runs on PyMol through the GlycoMe interface 🥰😍 check out this fab #glycotime tool from Hiren @glyco.me and Ojas @ojas-singh.bsky.social ⤵️🥳🥳🥳🎉🎉
Well, it turns out that you can actually make PyMOL do quite a lot of stuff. Here's the full workflow, integrating the amazing ReGlyco into PyMOL to re-glycosylate the LDLA linker. There's some serious magic needed to get it running as fast as it is within PyMOL. @elisafadda.bsky.social
June 11, 2025 at 6:33 AM
Too cool to be true? 😎 Nope! GlycoShape ReGlyco runs on PyMol through the GlycoMe interface 🥰😍 check out this fab #glycotime tool from Hiren @glyco.me and Ojas @ojas-singh.bsky.social ⤵️🥳🥳🥳🎉🎉
On my way to Lorne, Australia for #LorneProteomics2026. Looking forward to seeing the latest proteomics and glycomics advances.
I’ll be presenting our glycomics platform that more truthfully describes the glycome resulting in greater glycopeptide identifications.
Happy to meet up if you’re there!
I’ll be presenting our glycomics platform that more truthfully describes the glycome resulting in greater glycopeptide identifications.
Happy to meet up if you’re there!
February 5, 2026 at 12:04 AM
On my way to Lorne, Australia for #LorneProteomics2026. Looking forward to seeing the latest proteomics and glycomics advances.
I’ll be presenting our glycomics platform that more truthfully describes the glycome resulting in greater glycopeptide identifications.
Happy to meet up if you’re there!
I’ll be presenting our glycomics platform that more truthfully describes the glycome resulting in greater glycopeptide identifications.
Happy to meet up if you’re there!
Congratulations, fascinating #glycotime discovery connecting miRNA regulation of the glycome and proteome 👏
January 16, 2025 at 7:34 AM
Congratulations, fascinating #glycotime discovery connecting miRNA regulation of the glycome and proteome 👏
💡Join us for the SMART Symposium on Decoding the Glycome: Illuminating Biology's Last Uncharted Frontier
📆11–14 Nov 2026 | Shenzhen
🌏From glycan mapping to glyco‑engineered therapies
🔗Register: symposia.smart.org.cn
#SMARTSymposia #SMARTshenzhen #Glycome #Glycobiology
📆11–14 Nov 2026 | Shenzhen
🌏From glycan mapping to glyco‑engineered therapies
🔗Register: symposia.smart.org.cn
#SMARTSymposia #SMARTshenzhen #Glycome #Glycobiology
May 27, 2026 at 4:19 AM
💡Join us for the SMART Symposium on Decoding the Glycome: Illuminating Biology's Last Uncharted Frontier
📆11–14 Nov 2026 | Shenzhen
🌏From glycan mapping to glyco‑engineered therapies
🔗Register: symposia.smart.org.cn
#SMARTSymposia #SMARTshenzhen #Glycome #Glycobiology
📆11–14 Nov 2026 | Shenzhen
🌏From glycan mapping to glyco‑engineered therapies
🔗Register: symposia.smart.org.cn
#SMARTSymposia #SMARTshenzhen #Glycome #Glycobiology
Spatial Multiomics (Metabolome➡️ Lipidome➡️ Glycome) on a single 10 µm brain section👹
+Sami
Spatial Augmented Multiomics Interface
with Small Molecule Pathway Database
Direct pixel-to-pixel matching across modalities😎
@natcomms.nature.com 2025
www.nature.com/articles/s41...
+Sami
Spatial Augmented Multiomics Interface
with Small Molecule Pathway Database
Direct pixel-to-pixel matching across modalities😎
@natcomms.nature.com 2025
www.nature.com/articles/s41...
October 3, 2025 at 11:33 AM
Spatial Multiomics (Metabolome➡️ Lipidome➡️ Glycome) on a single 10 µm brain section👹
+Sami
Spatial Augmented Multiomics Interface
with Small Molecule Pathway Database
Direct pixel-to-pixel matching across modalities😎
@natcomms.nature.com 2025
www.nature.com/articles/s41...
+Sami
Spatial Augmented Multiomics Interface
with Small Molecule Pathway Database
Direct pixel-to-pixel matching across modalities😎
@natcomms.nature.com 2025
www.nature.com/articles/s41...
Now published in Nature Communications:
www.nature.com/articles/s41...
The anomer collapsing advance is something we continue to use today, and we hope others may find it useful.
Furthermore, this library is the largest representation to date of the human N-glycome (3x the next biggest) #glycotime
www.nature.com/articles/s41...
The anomer collapsing advance is something we continue to use today, and we hope others may find it useful.
Furthermore, this library is the largest representation to date of the human N-glycome (3x the next biggest) #glycotime
June 3, 2026 at 12:25 PM
Now published in Nature Communications:
www.nature.com/articles/s41...
The anomer collapsing advance is something we continue to use today, and we hope others may find it useful.
Furthermore, this library is the largest representation to date of the human N-glycome (3x the next biggest) #glycotime
www.nature.com/articles/s41...
The anomer collapsing advance is something we continue to use today, and we hope others may find it useful.
Furthermore, this library is the largest representation to date of the human N-glycome (3x the next biggest) #glycotime
@elisafadda.bsky.social we’ve seen quite substantial amounts of FA2/G0F glycans on (recombinant) corona Spike, flu HA, and ebola GP. Don’t think they’re that common in the general healthy human N-glycome.
November 22, 2024 at 11:25 PM
@elisafadda.bsky.social we’ve seen quite substantial amounts of FA2/G0F glycans on (recombinant) corona Spike, flu HA, and ebola GP. Don’t think they’re that common in the general healthy human N-glycome.
Congratulations on this exciting new #glycotime work! So many cool connections between the glycome and RNAome 😃
March 21, 2025 at 6:39 AM
Congratulations on this exciting new #glycotime work! So many cool connections between the glycome and RNAome 😃
First Bluesky post! Personal highlight of 2024, multi-glycomics method development that extracts multiple glycan types from the same sample for in-depth characterisation. #glycotime
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
SSSMuG: Same Sample Sequential Multi-Glycomics
The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as N- and O-linked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs), and other distinct gly...
pubs.acs.org
December 31, 2024 at 5:37 AM
First Bluesky post! Personal highlight of 2024, multi-glycomics method development that extracts multiple glycan types from the same sample for in-depth characterisation. #glycotime
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Comparative “omics” studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective of the brain N-glycome is lacking.
Human-specific features and developmental dynamics of the brain N-glycome 🏺 🧪
www.science.org/doi/10.1126/...
Human-specific features and developmental dynamics of the brain N-glycome 🏺 🧪
www.science.org/doi/10.1126/...
December 12, 2023 at 2:31 PM
Comparative “omics” studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective of the brain N-glycome is lacking.
Human-specific features and developmental dynamics of the brain N-glycome 🏺 🧪
www.science.org/doi/10.1126/...
Human-specific features and developmental dynamics of the brain N-glycome 🏺 🧪
www.science.org/doi/10.1126/...
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
Marglous et al. generated a database of anti-glycan antibody sequences, expressed and characterized a library of 154 antibodies, and generated DNA-barcoded antibodies for single-cell transcriptome and glycome profiling. Glycans associated with cancer stem cells and metastasis were uncovered, demonstrating utility for basic and clinical research.
dlvr.it
December 18, 2025 at 11:30 PM
Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies
High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes #nature #MassSpecRSS
High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes - Nature Communications
Glycomic analysis of scarce material remains a major challenge, limiting insight into glycosylation in human biology and disease. Here, the authors develop seGRAP-MS, an ultrasensitive and accessible workflow enabling single-oocyte glycome profiling that reveals a conserved human oocyte N-glycome.
dlvr.it
July 3, 2026 at 9:02 AM
High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes #nature #MassSpecRSS
In HS biology we learn about DNA, RNA, proteins. My @knowablemag.bsky.social story describes the other major building block of life: #glycans
knowablemagazine.org/content/arti...
knowablemagazine.org/content/arti...
Sweet! The cell’s sugary coating comes into view
Built from thickets of glucose, galactose, mannose and more, the glycome plays key roles in cell communication, immunity and the blood-brain barrier
knowablemagazine.org
March 24, 2026 at 3:59 PM
In HS biology we learn about DNA, RNA, proteins. My @knowablemag.bsky.social story describes the other major building block of life: #glycans
knowablemagazine.org/content/arti...
knowablemagazine.org/content/arti...