Unpause! I'm super happy to now be able to share the published version of our paper at Science Advances showing that:
1) active histone mods occur independently of transcription
2) transcription coordinates histone deacetylation at active promoters
www.science.org/doi/10.1126/...
1) active histone mods occur independently of transcription
2) transcription coordinates histone deacetylation at active promoters
www.science.org/doi/10.1126/...
RNA polymerase II coordinates histone deacetylation at active promoters
Transcription initiation limits histone acetylation and H2AZ incorporation at promoters.
www.science.org
February 12, 2025 at 9:21 PM
Unpause! I'm super happy to now be able to share the published version of our paper at Science Advances showing that:
1) active histone mods occur independently of transcription
2) transcription coordinates histone deacetylation at active promoters
www.science.org/doi/10.1126/...
1) active histone mods occur independently of transcription
2) transcription coordinates histone deacetylation at active promoters
www.science.org/doi/10.1126/...
Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2
tinyurl.com/setd2
December 12, 2024 at 7:16 PM
Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2
tinyurl.com/setd2
Thrilled to share our latest work, just published in @nature.com ⬇
www.nature.com/articles/s41...
We discovered that PARP inhibitors 💊 trigger histone eviction from the chromatin and this creates a hidden vulnerability in PARPi resistant tumors.
🧵 (1/8)
www.nature.com/articles/s41...
We discovered that PARP inhibitors 💊 trigger histone eviction from the chromatin and this creates a hidden vulnerability in PARPi resistant tumors.
🧵 (1/8)
NASP modulates histone turnover to drive PARP inhibitor resistance - Nature
PARP inhibitor treatment triggers histone release from the chromatin in cancer cells; consequently, targeting the histone chaperone NASP renders cells vulnerable to PARP inhibition.
www.nature.com
August 13, 2025 at 3:44 PM
Thrilled to share our latest work, just published in @nature.com ⬇
www.nature.com/articles/s41...
We discovered that PARP inhibitors 💊 trigger histone eviction from the chromatin and this creates a hidden vulnerability in PARPi resistant tumors.
🧵 (1/8)
www.nature.com/articles/s41...
We discovered that PARP inhibitors 💊 trigger histone eviction from the chromatin and this creates a hidden vulnerability in PARPi resistant tumors.
🧵 (1/8)
Excited to share our new preprint led by Fred and me in collaboration with the archaeal community! We found that the molecular foundation of histone-based chromatin has pre-eukaryotic roots in Asgard archaea. (1/4)
#ArchaeaSky
www.biorxiv.org/content/10.6...
#ArchaeaSky
www.biorxiv.org/content/10.6...
Emergence of histone-based chromatin complexity in Asgard archaea
The emergence of the eukaryotes coincided with the diversification of histone proteins and their post-translational modifications by enzymes that constitute the core of eukaryotic chromatin. Yet the e...
www.biorxiv.org
July 4, 2026 at 4:22 AM
Excited to share our new preprint led by Fred and me in collaboration with the archaeal community! We found that the molecular foundation of histone-based chromatin has pre-eukaryotic roots in Asgard archaea. (1/4)
#ArchaeaSky
www.biorxiv.org/content/10.6...
#ArchaeaSky
www.biorxiv.org/content/10.6...
Want to know how histone marks regulate your favorite gene? Go single molecule with ChromSMF: integrated measure of chromatin accessibility and histone marks + DNA methylation, TF footprints and genotype for free! Very proud of @mpalamin.bsky.social ! @embl.org
ChromSMF preprint is out!🚀
tinyurl.com/ChromSMF
We often piece together chromatin regulation layer by layer from separate assays. But this can be limiting!
In @arnaudkr.bsky.social's lab, we developed a method to directly study multiple layers on the same DNA molecule! 🧬
What does this unlock? ⬇️
tinyurl.com/ChromSMF
We often piece together chromatin regulation layer by layer from separate assays. But this can be limiting!
In @arnaudkr.bsky.social's lab, we developed a method to directly study multiple layers on the same DNA molecule! 🧬
What does this unlock? ⬇️
March 24, 2026 at 1:36 PM
Want to know how histone marks regulate your favorite gene? Go single molecule with ChromSMF: integrated measure of chromatin accessibility and histone marks + DNA methylation, TF footprints and genotype for free! Very proud of @mpalamin.bsky.social ! @embl.org
Work led by graduate student @trinitycookis.bsky.social and coauthors Alexandria Lydecker, @paulsauer.bsky.social, and @kasinath-lab.bsky.social is out today where we looked at the inhibition of PRC2 by histone PTMs associated with regions of active transcription.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural basis for the inhibition of PRC2 by active transcription histone posttranslational modifications - Nature Structural & Molecular Biology
Structures reveal that histone H3K36me3 and H3K4me3 modifications reduce Polycomb repressive complex 2 (PRC2) function through the inhibition of histone tail engagement and antagonistic binding to the...
www.nature.com
January 8, 2025 at 1:36 AM
Work led by graduate student @trinitycookis.bsky.social and coauthors Alexandria Lydecker, @paulsauer.bsky.social, and @kasinath-lab.bsky.social is out today where we looked at the inhibition of PRC2 by histone PTMs associated with regions of active transcription.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
𝐍𝐞𝐰 𝐦𝐞𝐜𝐡𝐚𝐧𝐢𝐬𝐭𝐢𝐜 𝐢𝐧𝐬𝐢𝐠𝐡𝐭𝐬 𝐢𝐧𝐭𝐨 𝐭𝐡𝐞 𝐫𝐞𝐠𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐡𝐢𝐬𝐭𝐨𝐧𝐞 𝐬𝐮𝐩𝐩𝐥𝐲! Excited to share our latest collaboration led by tkjeong.bsky.social and ciaranfrater.bsky.social! Structural determination of the histone chaperone ASF1 and its regulator CODANIN-1 uncovers how CODANIN-1 counters ASF1 activity. 🧵👇
CODANIN-1 sequesters ASF1 by using a histone H3 mimic helix to regulate the histone supply
Nature Communications - CODANIN-1 negatively regulates the function of ASF1, the key chaperone for histone H3-H4 supply. Here the authors present the cryo-EM structure of the CODANIN-1_ASF1A...
rdcu.be
March 6, 2025 at 12:45 PM
𝐍𝐞𝐰 𝐦𝐞𝐜𝐡𝐚𝐧𝐢𝐬𝐭𝐢𝐜 𝐢𝐧𝐬𝐢𝐠𝐡𝐭𝐬 𝐢𝐧𝐭𝐨 𝐭𝐡𝐞 𝐫𝐞𝐠𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐡𝐢𝐬𝐭𝐨𝐧𝐞 𝐬𝐮𝐩𝐩𝐥𝐲! Excited to share our latest collaboration led by tkjeong.bsky.social and ciaranfrater.bsky.social! Structural determination of the histone chaperone ASF1 and its regulator CODANIN-1 uncovers how CODANIN-1 counters ASF1 activity. 🧵👇
Ever wondered what the lonely histone H3C110 does? We found that it’s lost in many fungi. Adding it back boosts histone H3 copper reductase activity, rescues iron defects and modulates lifespan, suggesting an evolutionary tradeoff between these effects.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Histone H3 cysteine 110 enhances iron metabolism and modulates replicative life span in Saccharomyces cerevisiae
Histone H3 cysteine 110 enhances iron metabolism and modulates replicative life span in yeast.
www.science.org
April 11, 2025 at 9:43 PM
Ever wondered what the lonely histone H3C110 does? We found that it’s lost in many fungi. Adding it back boosts histone H3 copper reductase activity, rescues iron defects and modulates lifespan, suggesting an evolutionary tradeoff between these effects.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Our story on the mechanism of co-transcriptional histone mark deposition is now officially out: www.science.org/doi/10.1126/...
Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription
During transcription, RNA polymerase II traverses through chromatin, and posttranslational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine ...
www.science.org
January 30, 2025 at 10:16 PM
Our story on the mechanism of co-transcriptional histone mark deposition is now officially out: www.science.org/doi/10.1126/...
1/ Very excited to share the first publication from my lab!
In this work, we developed an efficient strategy to precisely mutate mammalian histone genes in their native genomic context using CRISPR prime editing: www.nature.com/articles/s41...
In this work, we developed an efficient strategy to precisely mutate mammalian histone genes in their native genomic context using CRISPR prime editing: www.nature.com/articles/s41...
Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing - Nature Genetics
This study uses a precise and efficient clustered regularly interspaced short palindromic repeats (CRISPR) prime editing system to substitute lysine residues in histone H3, individually or in combinat...
www.nature.com
July 8, 2026 at 10:05 AM
1/ Very excited to share the first publication from my lab!
In this work, we developed an efficient strategy to precisely mutate mammalian histone genes in their native genomic context using CRISPR prime editing: www.nature.com/articles/s41...
In this work, we developed an efficient strategy to precisely mutate mammalian histone genes in their native genomic context using CRISPR prime editing: www.nature.com/articles/s41...
Please RS/RT. #GordonConference on "Histone and DNA Modifications" #epigenetics #chromatin 20-25. July 2025. Il Ciocco, Italy. Great speaker lineup.
➡️Register now www.grc.org/histone-and-...
➡️Register now www.grc.org/histone-and-...
December 8, 2024 at 11:38 AM
Please RS/RT. #GordonConference on "Histone and DNA Modifications" #epigenetics #chromatin 20-25. July 2025. Il Ciocco, Italy. Great speaker lineup.
➡️Register now www.grc.org/histone-and-...
➡️Register now www.grc.org/histone-and-...
So excited that our work on predicting gene expression from histone modifications using deep learning is out in NAR today. Brilliant to work with lead author @al-murphy.bsky.social and collaborators Aydan Askarova, @borislenhard.bsky.social and Nathan Skene 🧬⭐️🙏
academic.oup.com/nar/advance-...
academic.oup.com/nar/advance-...
Predicting gene expression from histone marks using chromatin deep learning models depends on histone mark function, regulatory distance and cellular states
Abstract. To understand the complex relationship between histone mark activity and gene expression, recent advances have used in silico predictions based o
academic.oup.com
December 11, 2024 at 5:02 PM
So excited that our work on predicting gene expression from histone modifications using deep learning is out in NAR today. Brilliant to work with lead author @al-murphy.bsky.social and collaborators Aydan Askarova, @borislenhard.bsky.social and Nathan Skene 🧬⭐️🙏
academic.oup.com/nar/advance-...
academic.oup.com/nar/advance-...
TaHDA1-mediated #histone and non-histone #deacetylation orchestrates #drought tolerance in #wheat
onlinelibrary.wiley.com/doi/10.1111/...
#PlantScience @jipb.bsky.social @wileylifesci.bsky.social @wileyecology.bsky.social @planteditors.bsky.social @plant-sci.bsky.social @wheatpath.bsky.social
onlinelibrary.wiley.com/doi/10.1111/...
#PlantScience @jipb.bsky.social @wileylifesci.bsky.social @wileyecology.bsky.social @planteditors.bsky.social @plant-sci.bsky.social @wheatpath.bsky.social
TaHDA1‐mediated histone and non‐histone deacetylation orchestrates drought tolerance in wheat
The histone deacetylase TaHDA1 is a key regulator linking histone and non-histone deacetylation with drought adaptation and a potential target for improving drought resilience and grain nutritional q....
onlinelibrary.wiley.com
September 17, 2026 at 12:43 PM
TaHDA1-mediated #histone and non-histone #deacetylation orchestrates #drought tolerance in #wheat
onlinelibrary.wiley.com/doi/10.1111/...
#PlantScience @jipb.bsky.social @wileylifesci.bsky.social @wileyecology.bsky.social @planteditors.bsky.social @plant-sci.bsky.social @wheatpath.bsky.social
onlinelibrary.wiley.com/doi/10.1111/...
#PlantScience @jipb.bsky.social @wileylifesci.bsky.social @wileyecology.bsky.social @planteditors.bsky.social @plant-sci.bsky.social @wheatpath.bsky.social
Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...
@mpi-bio-fml.bsky.social
@mpi-bio-fml.bsky.social
May 12, 2025 at 9:17 AM
Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...
@mpi-bio-fml.bsky.social
@mpi-bio-fml.bsky.social
Publication Alert! Happy to share our new work published in CellReports of a collaboration with the Zychlinsky Lab (Max Planck Institute for Infection Biology). "Histone H1 kills MRSA". cell.com/cell-reports.... Enjoy!
Histone H1 kills MRSA
Marsman et al. detect histone H1 in MRSA in human abscesses and demonstrate that it
kills MRSA under physiological conditions. They identify through selective evolution
and a genome-wide screen that h...
cell.com
November 15, 2024 at 12:28 PM
Publication Alert! Happy to share our new work published in CellReports of a collaboration with the Zychlinsky Lab (Max Planck Institute for Infection Biology). "Histone H1 kills MRSA". cell.com/cell-reports.... Enjoy!
A must-read for histone afficionados: our new review on 'Histone-mediated chromatin organization in prokaryotes and viruses' www.cell.com/trends/bioch... @samuelschwab.bsky.social @vikramalva.bsky.social
Histone-mediated chromatin organization in prokaryotes and viruses
Histones are fundamental chromatin-organizing proteins in eukaryotes and archaea,
where they assemble into (hyper)nucleosomes that wrap DNA. Recent studies have expanded
the known repertoire of histon...
www.cell.com
June 30, 2025 at 7:35 AM
A must-read for histone afficionados: our new review on 'Histone-mediated chromatin organization in prokaryotes and viruses' www.cell.com/trends/bioch... @samuelschwab.bsky.social @vikramalva.bsky.social
My favorite science is the kind that makes you says, "Man, I wish I thought of that!" This is such a paper.
Mass action turns an eraser into a pen. HDACs are metabolite-dependent histone acyltransferases.
@dremilygoldberg.bsky.social @gburslem.bsky.social
www.nature.com/articles/s41...
Mass action turns an eraser into a pen. HDACs are metabolite-dependent histone acyltransferases.
@dremilygoldberg.bsky.social @gburslem.bsky.social
www.nature.com/articles/s41...
Reversible histone deacetylase activity catalyzes lysine acylation - Nature Chemical Biology
Tsusaka et al. discover that histone deacetylases, which are well known to remove protein modifications, such as lysine acetylation and β-hydroxybutyrylation, can also reverse their chemical activity ...
www.nature.com
March 30, 2025 at 5:28 PM
My favorite science is the kind that makes you says, "Man, I wish I thought of that!" This is such a paper.
Mass action turns an eraser into a pen. HDACs are metabolite-dependent histone acyltransferases.
@dremilygoldberg.bsky.social @gburslem.bsky.social
www.nature.com/articles/s41...
Mass action turns an eraser into a pen. HDACs are metabolite-dependent histone acyltransferases.
@dremilygoldberg.bsky.social @gburslem.bsky.social
www.nature.com/articles/s41...
Histone modifications in development
Read this 'Development at a Glance' article by Yu-Hao Liu and @robertife.bsky.social, summarising the role of selected histone modifications during development.
journals.biologists.com/dev/article/...
Read this 'Development at a Glance' article by Yu-Hao Liu and @robertife.bsky.social, summarising the role of selected histone modifications during development.
journals.biologists.com/dev/article/...
June 18, 2025 at 8:51 AM
Histone modifications in development
Read this 'Development at a Glance' article by Yu-Hao Liu and @robertife.bsky.social, summarising the role of selected histone modifications during development.
journals.biologists.com/dev/article/...
Read this 'Development at a Glance' article by Yu-Hao Liu and @robertife.bsky.social, summarising the role of selected histone modifications during development.
journals.biologists.com/dev/article/...
Nature research paper: NASP modulates histone turnover to drive PARP inhibitor resistance
go.nature.com/45vRWhd
go.nature.com/45vRWhd
NASP modulates histone turnover to drive PARP inhibitor resistance - Nature
PARP inhibitor treatment triggers histone release from the chromatin in cancer cells; consequently, targeting the histone chaperone NASP renders cells vulnerable to PARP inhibition.
go.nature.com
August 18, 2025 at 1:31 PM
Nature research paper: NASP modulates histone turnover to drive PARP inhibitor resistance
go.nature.com/45vRWhd
go.nature.com/45vRWhd
Our review on ➡️"Histone modifications in development" is out 🍾 in 🔖 Development @dev-journal.bsky.social urnal.bsky.social
Read more about histone tail and globular domain modifications during development: journals.biologists.com/dev/article/...
Read more about histone tail and globular domain modifications during development: journals.biologists.com/dev/article/...
Histone modifications in development
Read this 'Development at a Glance' article by Yu-Hao Liu and @robertife.bsky.social, summarising the role of selected histone modifications during development.
journals.biologists.com/dev/article/...
Read this 'Development at a Glance' article by Yu-Hao Liu and @robertife.bsky.social, summarising the role of selected histone modifications during development.
journals.biologists.com/dev/article/...
June 19, 2025 at 10:22 AM
Our review on ➡️"Histone modifications in development" is out 🍾 in 🔖 Development @dev-journal.bsky.social urnal.bsky.social
Read more about histone tail and globular domain modifications during development: journals.biologists.com/dev/article/...
Read more about histone tail and globular domain modifications during development: journals.biologists.com/dev/article/...
Read our recent publication in the Journal of Proteome Research on an approach to improve the quantification of histone ubiquitination.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Optimized and Robust Workflow for Quantifying the Canonical Histone Ubiquitination Marks H2AK119ub and H2BK120ub by LC–MS/MS
The eukaryotic genome is packaged around histone proteins, which are subject to a myriad of post-translational modifications. By controlling DNA accessibility and the recruitment of protein complexes ...
pubs.acs.org
November 23, 2024 at 4:57 PM
Read our recent publication in the Journal of Proteome Research on an approach to improve the quantification of histone ubiquitination.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Happy to share that our work on HLp, a bacterial histone from Leptospira perolatii, is now published in Nature Communications 🎉
In this study, we show that HLp forms stable tetramers that wrap ~60 bp of DNA, revealing a distinct histone–DNA organization in bacteria.
www.nature.com/articles/s41...
In this study, we show that HLp forms stable tetramers that wrap ~60 bp of DNA, revealing a distinct histone–DNA organization in bacteria.
www.nature.com/articles/s41...
December 13, 2025 at 8:09 AM
Happy to share that our work on HLp, a bacterial histone from Leptospira perolatii, is now published in Nature Communications 🎉
In this study, we show that HLp forms stable tetramers that wrap ~60 bp of DNA, revealing a distinct histone–DNA organization in bacteria.
www.nature.com/articles/s41...
In this study, we show that HLp forms stable tetramers that wrap ~60 bp of DNA, revealing a distinct histone–DNA organization in bacteria.
www.nature.com/articles/s41...
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
May 26, 2025 at 4:56 PM
We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM
Interested in the unexpected? How about a link between piRNAs and maternal histone mRNAs … Great collab with @sebastianfalk.bsky.social, @koenig-lab.bsky.social and Florian Steiner.
#piRNA #histone #mRNA #maternal #RNAbiology #RNASky
www.biorxiv.org/content/10.6...
#piRNA #histone #mRNA #maternal #RNAbiology #RNASky
www.biorxiv.org/content/10.6...
www.biorxiv.org
January 8, 2026 at 8:51 AM
Interested in the unexpected? How about a link between piRNAs and maternal histone mRNAs … Great collab with @sebastianfalk.bsky.social, @koenig-lab.bsky.social and Florian Steiner.
#piRNA #histone #mRNA #maternal #RNAbiology #RNASky
www.biorxiv.org/content/10.6...
#piRNA #histone #mRNA #maternal #RNAbiology #RNASky
www.biorxiv.org/content/10.6...