Crystal Pharmatech announces the operational launch of the ELDICO ED-1 electron diffractometer at its Suzhou facility, enhancing global MicroED services.

スペラファーマが協賛する第2回MicroED/3D EDユーザーズミーティングが開催。製薬研究の最新知見が共有される予定です。

スペラファーマが協賛する第2回MicroED/3D EDユーザーズミーティングが開催。製薬研究の最新知見が共有される予定です。

AbstractDetermining the three-dimensional structures of small molecules in complex mixtures remains challenging as current approaches typically require physical isolation and quantities of material that are excessive for many components. Here we present a reciprocal structure-determination strategy that integrates ion mobility-resolved high-resolution tandem mass spectrometry (IMS-HRMS-MS/MS) with microcrystal electron diffraction (MicroED/3DED), enabling chemically consistent three-dimensional structure elucidation from sub-microgram quantities of material. Analysis of synthetic reaction mixtures revealed multiple stereoisomeric products with distinct ion-mobility and fragmentation behaviour. Mechanistically grounded modelling of fragmentation pathways and collision cross sections constrained viable three-dimensional molecular geometries, while MicroED independently resolved the structure of a crystallographically accessible isomer. Crucially, the gas-phase-derived geometries served directly as molecular replacement search models for MicroED, including for a second reaction product whose diffraction data could not be solved by direct methods alone, thereby demonstrating direct transfer of structural information between mass spectrometry and electron diffraction. Together, IMS-HRMS-MS/MS and MicroED provide complementary structural projections whose integrated interpretation enables structure elucidation of small molecules directly from complex heterogeneous mixtures using minimal amounts of material.

Hydrogen—the lightest atom—does the heavy lifting when it comes to important chemical reactions. It can be stored and released as clean fuel, and its movements help catalysts build medicines, fertilizers and materials.

MicroED has become a powerful tool for determining the atomic structure of microscale and nanoscale crystals, particularly in the pharmaceutical industry.

We present an algorithm for unit-cell determination from a set of randomly oriented electron diffraction patterns and demonstrate its performance for two known structures (copper perchlorophthalocyanine and lysozyme) and one as-yet uncharacterized structure.

While purifying the seed protein crambin, we discovered that needles of pure protein nanocrystals formed spontaneously during the drying of a simple ethanolic purification drop. Contrary to traditiona...

With the goal of accelerating the discovery of small molecule-protein complexes, we leverage fast, low-dose, event based electron counting microcrystal electron diffraction (MicroED) data collection a...

Alt: a cartoon of a monkey with the words ratioed + bloon sabotaged + i have more black borders than you

The Glacios 3 Cryo TEM is a high-performance cryo electron microscope for single particle analysis, cryo-electron tomography, and microED.