In this work, nanocrystals of a robust switchable SCO-MOF are successfully synthesized using a sonication strategy. Microcrystal Electron Diffraction (MicroED) confirmed the preservation of structura...

High-resolution data are crucial for accurate structural modeling. Here, authors enhance MicroED data quality using energy filtering, achieving sub-atomic resolution protein data and uncovering diffus...

Microcrystal electron diffraction microED promises revolution study proteins small molecules

High-resolution data are crucial for accurate structural modeling. Here, authors enhance MicroED data quality using energy filtering, achieving sub-atomic resolution protein data and uncovering diffus...

A three-day school of lectures and practical sessions on electron crystallography methods

With an increasing number of refined MicroED structures available to the community, lessons can be learned of their profiles and quality, as well as of best practices.

Here we identify and characterize the warping of molecular crystal lattices induced by electron beam exposure during microcrystal electron diffraction (MicroED/3DED) data collection. We find changes to consensus crystal lattice orientation that are often dramatic, and appear ubiquitous in small organic molecule crystals. This evidence highlights the relevance of crystal bending or warping as a consequence of radiation-induced damage on molecular specimens, and points to it as a fundamental source of error in MicroED/3DED data collection and structure determination.

The effect of radiation damage in MicroED has been studied. By only including data collected at low accumulated electron dose during data processing, the effects of radiation damage can be reduced.