精密に測るほど短くなる蛍光の軌跡。大庭紗季と栗原透馬が、人工の輸送補助分子〈渡し舟〉を追う。MINFLUXに着想を得た創作。本文・挿絵に生成AIを使用。

Co-founded by Nobel Laureate Stefan W. Hell, the chief inventor of STED and MINFLUX, abberior is the go-to source for superresolution microscopes and dyes.

Slivka et al. track dynein’s microtubule-binding domain with minimal fluorescence photon flux, revealing transient backward ‘dips’ before steps. These dips represent microtubule release upon ATP bindi...

Wang et al. introduce HoTs, self-blinking DNA probes for live-cell super-resolution chromatin imaging. Compatible with both STORM and MINFLUX microscopy, HoTs capture chromatin organization across live cells, animal tissues, and clinical tumor samples, opening new opportunities for studying genome architecture in health and disease.

Wang et al. introduce HoTs, self-blinking DNA probes for live-cell super-resolution chromatin imaging. Compatible with both STORM and MINFLUX microscopy, HoTs capture chromatin organization across live cells, animal tissues, and clinical tumor samples, opening new opportunities for studying genome architecture in health and disease.

Nature Structural & Molecular Biology, Published online: 26 August 2026; doi:10.1038/s41594-026-01873-wSlivka et al. track dynein’s microtubule-binding domain with minimal fluorescence photon flux, revealing transient backward ‘dips’ before steps. These dips represent microtubule release upon ATP binding and diffusional search, terminating in net forward bias driven by ATP hydrolysis.

a, The schematic of yeast dynein dimer bound to the MT. The dashed rectangle shows the motor domain of the dynein heavy chain. b, Denaturing gels of GST-dynein constructs labeled with Alexa647-maleimide under the same experimental conditions imaged by (Left) fluorescence and (Right) Coomassie staining (MWM: molecular weight marker; kDa: kilodalton; 5 biological replicates). The bands in 27 and 43 kDa correspond to Tev protease and maltose binding protein (MBP) from the Tev-MBP construct used to elute the protein from affinity beads during preparation. c, The denaturing gel picture shows the mixing of SpyCatcher-DynCLM and SpyTag-DynCLM at a 5:1 ratio to result in the heterodimerization of these constructs (black arrow; 4 biological replicates). d, (Inset) Diffraction-limited images of a dynein heterodimer labeled with a single LD655 dye at its MTBD. Trajectories show FIONA tracking of dynein heterodimers with 100 ms temporal resolution at 2 µM ATP. The red lines show a fit for the step-finding algorithm. e, The histogram of steps taken along the MT long- and short-axis. Dashed lines show increments of 8 nm steps. f, Velocity and run length characteristics of WT and CLM Q3231C at saturating (1 mM) ATP. The centerline, box, and whiskers represent the mean,...

Nature Structural & Molecular Biology, Published online: 26 August 2026; doi:10.1038/s41594-026-01873-wSlivka et al. track dynein’s microtubule-binding domain with minimal fluorescence photon flux, revealing transient backward ‘dips’ before steps. These dips represent microtubule release upon ATP binding and diffusional search, terminating in net forward bias driven by ATP hydrolysis.

Neurotransmitter release and membrane retrieval at active zones require precise spatial and temporal coordination relying on an intricate molecular machinery. However, the exact nano-structural organi...