Woah! Sick reconstitution work showing that MAP6d1 recruits tubulin to form B tubules on A tubule templates, and ALSO intra-luminal protofilaments, which apparently are present in neuronal microtubules.
So not only actin inside microtubules, but also additional tubulin protofilaments?!? Wild!
So not only actin inside microtubules, but also additional tubulin protofilaments?!? Wild!
July 6, 2025 at 4:33 PM
Woah! Sick reconstitution work showing that MAP6d1 recruits tubulin to form B tubules on A tubule templates, and ALSO intra-luminal protofilaments, which apparently are present in neuronal microtubules.
So not only actin inside microtubules, but also additional tubulin protofilaments?!? Wild!
So not only actin inside microtubules, but also additional tubulin protofilaments?!? Wild!
Happy to contribute to this fantastic work by @maksimkalutskii.bsky.social and @maxotubule.bsky.social!
www.pnas.org/doi/abs/10.1...
www.pnas.org/doi/abs/10.1...
June 3, 2025 at 11:14 AM
Happy to contribute to this fantastic work by @maksimkalutskii.bsky.social and @maxotubule.bsky.social!
www.pnas.org/doi/abs/10.1...
www.pnas.org/doi/abs/10.1...
BTW we‘ve recently released an improved version of our manuscript uncovering the assembly mechanism of microtubules via intermediate clusters of protofilaments!
@maksimkalutskii.bsky.social @vladimirvolkov.bsky.social @compbiophys.bsky.social
👇👇👇
doi.org/10.1101/2024...
@maksimkalutskii.bsky.social @vladimirvolkov.bsky.social @compbiophys.bsky.social
👇👇👇
doi.org/10.1101/2024...
December 7, 2024 at 8:03 PM
BTW we‘ve recently released an improved version of our manuscript uncovering the assembly mechanism of microtubules via intermediate clusters of protofilaments!
@maksimkalutskii.bsky.social @vladimirvolkov.bsky.social @compbiophys.bsky.social
👇👇👇
doi.org/10.1101/2024...
@maksimkalutskii.bsky.social @vladimirvolkov.bsky.social @compbiophys.bsky.social
👇👇👇
doi.org/10.1101/2024...
What is THIS?
Source: doi.org/10.1016/j.cr...
Source: doi.org/10.1016/j.cr...
September 21, 2026 at 5:51 PM
What is THIS?
Source: doi.org/10.1016/j.cr...
Source: doi.org/10.1016/j.cr...
Congratulations to Doi Basu @dwaipayanbasu.bsky.social for publishing his first first-author paper from the lab!
The cryoEM structure of a tubulin-like protein from bacteriophage Goslar forms "microtubules" with nine protofilaments!
authors.elsevier.com/a/1mM253SNvc...
The cryoEM structure of a tubulin-like protein from bacteriophage Goslar forms "microtubules" with nine protofilaments!
authors.elsevier.com/a/1mM253SNvc...
authors.elsevier.com
December 30, 2025 at 5:35 AM
Congratulations to Doi Basu @dwaipayanbasu.bsky.social for publishing his first first-author paper from the lab!
The cryoEM structure of a tubulin-like protein from bacteriophage Goslar forms "microtubules" with nine protofilaments!
authors.elsevier.com/a/1mM253SNvc...
The cryoEM structure of a tubulin-like protein from bacteriophage Goslar forms "microtubules" with nine protofilaments!
authors.elsevier.com/a/1mM253SNvc...
Is the actin twist the highest resolution feature that can be observed in a raw tomogram? Maybe the protofilaments of microtubules? #teamtomo
November 20, 2024 at 11:58 PM
Is the actin twist the highest resolution feature that can be observed in a raw tomogram? Maybe the protofilaments of microtubules? #teamtomo
Nice, some MTs splaying to individual protofilaments upon bending with two optical traps.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
September 12, 2025 at 10:18 AM
Nice, some MTs splaying to individual protofilaments upon bending with two optical traps.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Performing cryoET on microtubules stabilised on grids in this way, we found that stabilised microtubule ends carry short and clustered protofilaments - properties that we previously described as characteristic for growing microtubules, despite the fact that microtubules were actually shortening(3/7)
July 7, 2025 at 9:39 AM
Performing cryoET on microtubules stabilised on grids in this way, we found that stabilised microtubule ends carry short and clustered protofilaments - properties that we previously described as characteristic for growing microtubules, despite the fact that microtubules were actually shortening(3/7)
🍃 🦠 🧪 Bacteriophage Microtubule-like Filaments
Rigid tubes formed by nine nearly straight protofilaments
Rigid tubes formed by nine nearly straight protofilaments
December 30, 2025 at 2:22 PM
🍃 🦠 🧪 Bacteriophage Microtubule-like Filaments
Rigid tubes formed by nine nearly straight protofilaments
Rigid tubes formed by nine nearly straight protofilaments
Have a great D2@ #EBSA2025 in Rome🫠! Here are the posters of today's session: 📌Maksim @maksimkalutskii.bsky.social is presenting (P-2.195) his project addressing #Microtubule Dynamics Are Defined by Conformations and Stability of Clustered #Protofilaments 📜🔗 www.pnas.org/doi/10.1073/...
Microtubule dynamics are defined by conformations and stability of clustered protofilaments | PNAS
Microtubules are dynamic cytoskeletal polymers that add and lose tubulin dimers at
their ends. Microtubule growth, shortening, and transitions betw...
www.pnas.org
July 2, 2025 at 7:32 AM
Have a great D2@ #EBSA2025 in Rome🫠! Here are the posters of today's session: 📌Maksim @maksimkalutskii.bsky.social is presenting (P-2.195) his project addressing #Microtubule Dynamics Are Defined by Conformations and Stability of Clustered #Protofilaments 📜🔗 www.pnas.org/doi/10.1073/...
Low-resolution subtomogram averaging revealed ordered "trains" of Ndc80 along tubulin protofilaments, but now ordered binding of Ska (4/7)
July 7, 2025 at 9:39 AM
Low-resolution subtomogram averaging revealed ordered "trains" of Ndc80 along tubulin protofilaments, but now ordered binding of Ska (4/7)
Mamata Bangera, iitmadras shows cryoEM of structures of microtubules from Plasmodium. Protofilaments in Plasmodium microtubules are stiffer. Properties may lead to the observed innate variation in protofilament number in microtubules. #M2T22025
February 17, 2025 at 7:14 AM
Mamata Bangera, iitmadras shows cryoEM of structures of microtubules from Plasmodium. Protofilaments in Plasmodium microtubules are stiffer. Properties may lead to the observed innate variation in protofilament number in microtubules. #M2T22025
Polyubiquitin may shape tau filaments beyond their ordered core: shifts in its position altered the interface between two protofilaments, producing previously unobserved structures. doi.org/hcmjhd
Polyubiquitin may stabilize tau filament structures in neurodegenerative diseases
In neurodegenerative diseases such as Alzheimer's disease, progressive neuronal damage is associated with the accumulation of abnormal tau filaments in the brain.
medicalxpress.com
October 4, 2026 at 1:00 PM
Polyubiquitin may shape tau filaments beyond their ordered core: shifts in its position altered the interface between two protofilaments, producing previously unobserved structures. doi.org/hcmjhd
also happy to report it gets worse. I took it up on the suggestion to make a cross section, and then its suggestion to label the 13 protofilaments.
September 4, 2026 at 1:33 AM
also happy to report it gets worse. I took it up on the suggestion to make a cross section, and then its suggestion to label the 13 protofilaments.
Happy to have Youngseo Lee join #ComeInCell to work in Montenegro's lab.
She will explore how self-assembling #peptide amphiphiles form #protofilaments under confinement its interaction with #membranes. More details here: www.comeincell.org/dc09-synthet...
Welcome, Youngseo! #HorizonEurope #MSCA
She will explore how self-assembling #peptide amphiphiles form #protofilaments under confinement its interaction with #membranes. More details here: www.comeincell.org/dc09-synthet...
Welcome, Youngseo! #HorizonEurope #MSCA
September 2, 2025 at 12:14 PM
Happy to have Youngseo Lee join #ComeInCell to work in Montenegro's lab.
She will explore how self-assembling #peptide amphiphiles form #protofilaments under confinement its interaction with #membranes. More details here: www.comeincell.org/dc09-synthet...
Welcome, Youngseo! #HorizonEurope #MSCA
She will explore how self-assembling #peptide amphiphiles form #protofilaments under confinement its interaction with #membranes. More details here: www.comeincell.org/dc09-synthet...
Welcome, Youngseo! #HorizonEurope #MSCA
Lovely work from the lab of one of my favourite people in the world - On the role of nucleotides and lipids in the polymerization of the actin homolog MreB from a Gram-positive bacterium elifesciences.org/articles/845...
On the role of nucleotides and lipids in the polymerization of the actin homolog MreB from a Gram-po...
First 3D-structure and first observation of organized protofilaments of MreB from a Gram-positive bacterium, suggesting a model where ATP drives the formation of dynamic pairs of MreB protofilaments o...
elifesciences.org
December 18, 2023 at 7:40 PM
Lovely work from the lab of one of my favourite people in the world - On the role of nucleotides and lipids in the polymerization of the actin homolog MreB from a Gram-positive bacterium elifesciences.org/articles/845...
Interface integrity in septin protofilaments is maintained by an arginine residue conserved from yeast to man. https://www.biorxiv.org/content/10.1101/2025.01.29.635471v1
January 30, 2025 at 2:46 AM
Interface integrity in septin protofilaments is maintained by an arginine residue conserved from yeast to man. https://www.biorxiv.org/content/10.1101/2025.01.29.635471v1
Eventually, yes.. Extrapolating ad absurdo, we can probably agree that centrosome is also a huge cluster of protofilaments with an intricate organisation
July 9, 2025 at 7:41 AM
Eventually, yes.. Extrapolating ad absurdo, we can probably agree that centrosome is also a huge cluster of protofilaments with an intricate organisation
That’s fabulous! I would love to walk by that every day.
How many protofilaments? There’s no seam visible, so not a 13pf microtubule. Right?
How many protofilaments? There’s no seam visible, so not a 13pf microtubule. Right?
January 12, 2026 at 4:28 AM
That’s fabulous! I would love to walk by that every day.
How many protofilaments? There’s no seam visible, so not a 13pf microtubule. Right?
How many protofilaments? There’s no seam visible, so not a 13pf microtubule. Right?
Then what stops it from diffusing off the tip? One argument against a diffusive ring is that it will slide right off a growing tip, which lacks large curled protofilaments (PFs) to sterically block it. We find that this dynamic interface itself creates a substantial barrier to ring escape. 6/n
September 23, 2026 at 3:30 PM
Then what stops it from diffusing off the tip? One argument against a diffusive ring is that it will slide right off a growing tip, which lacks large curled protofilaments (PFs) to sterically block it. We find that this dynamic interface itself creates a substantial barrier to ring escape. 6/n
2/4 We identified two FtsZ paralogs—OdinFtsZ1 & OdinFtsZ2—with distinct structures. OdinFtsZ1 forms protofilaments, like bacterial FtsZ, while OdinFtsZ2 forms stacked rings, similar to OdinTubulin! Shared function? 🤔
February 4, 2025 at 5:55 AM
2/4 We identified two FtsZ paralogs—OdinFtsZ1 & OdinFtsZ2—with distinct structures. OdinFtsZ1 forms protofilaments, like bacterial FtsZ, while OdinFtsZ2 forms stacked rings, similar to OdinTubulin! Shared function? 🤔
See their @elife.bsky.social paper on MreB and the role of nucleotides and lipids
elifesciences.org/articles/84505
#BACELL2025
elifesciences.org/articles/84505
#BACELL2025
On the role of nucleotides and lipids in the polymerization of the actin homolog MreB from a Gram-positive bacterium
First 3D-structure and first observation of organized protofilaments of MreB from a Gram-positive bacterium, suggesting a model where ATP drives the formation of dynamic pairs of MreB protofilaments o...
elifesciences.org
June 17, 2025 at 11:54 AM
See their @elife.bsky.social paper on MreB and the role of nucleotides and lipids
elifesciences.org/articles/84505
#BACELL2025
elifesciences.org/articles/84505
#BACELL2025
A paper using RRID:SCR_004097 was just published in iScience see "A new polymorphism of human amylin fibrils with similar protofilaments and a conserved core". We appreciate the author's support of reproducibility. #ReproducibleResearch #accelerateopenscience
doi.org
April 10, 2025 at 7:00 AM
A paper using RRID:SCR_004097 was just published in iScience see "A new polymorphism of human amylin fibrils with similar protofilaments and a conserved core". We appreciate the author's support of reproducibility. #ReproducibleResearch #accelerateopenscience
Cryo-EM resolves the fibers at 1.7 Å, revealing tightly packed protofilaments, ordered water channels, and a unidirectional dipole along the fiber. Together, aromatic stacking, polar interactions, and water organization generate emergent electrostatics and mechanical resilience.
August 7, 2026 at 5:25 AM
Cryo-EM resolves the fibers at 1.7 Å, revealing tightly packed protofilaments, ordered water channels, and a unidirectional dipole along the fiber. Together, aromatic stacking, polar interactions, and water organization generate emergent electrostatics and mechanical resilience.