転写共役DNA修復の仕組みに迫る
DNA損傷によりRNAPolIIが転写中に停止することで開始する転写共役DNA修復を初めて試験管内で再構成。AlphaFold予測などによりSTK19という因子が修復マシナリーのリクルートに重要であることを発見。Cockayne症候群などの関連疾患の理解に重要
DNA損傷によりRNAPolIIが転写中に停止することで開始する転写共役DNA修復を初めて試験管内で再構成。AlphaFold予測などによりSTK19という因子が修復マシナリーのリクルートに重要であることを発見。Cockayne症候群などの関連疾患の理解に重要
www.cell.com
March 12, 2025 at 10:00 AM
転写共役DNA修復の仕組みに迫る
DNA損傷によりRNAPolIIが転写中に停止することで開始する転写共役DNA修復を初めて試験管内で再構成。AlphaFold予測などによりSTK19という因子が修復マシナリーのリクルートに重要であることを発見。Cockayne症候群などの関連疾患の理解に重要
DNA損傷によりRNAPolIIが転写中に停止することで開始する転写共役DNA修復を初めて試験管内で再構成。AlphaFold予測などによりSTK19という因子が修復マシナリーのリクルートに重要であることを発見。Cockayne症候群などの関連疾患の理解に重要
Do you know where transcription of a gene actually stops? Hint: it is not the annotated gene end.
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...
December 29, 2024 at 11:21 AM
We just posted a preprint about a death mechanism that we find truly surprising.
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
Pol II degradation activates cell death independently from the loss of transcription
Pol II-mediated transcription is essential for eukaryotic life. While loss of transcription is thought to be universally lethal, the associated mechanisms promoting cell death are not yet known. Here,...
www.biorxiv.org
December 13, 2024 at 4:02 PM
Another noteworthy work from 2025 is the use of RIP to isolate actively transcribing #RNAPolII proteins for #CryoEM, in order to obtain new information on structural dynamics during elongation. www.nature.com/articles/s41...
www.nature.com
December 4, 2025 at 7:20 AM
Another noteworthy work from 2025 is the use of RIP to isolate actively transcribing #RNAPolII proteins for #CryoEM, in order to obtain new information on structural dynamics during elongation. www.nature.com/articles/s41...
⭐ #NARBreakthrough! ⭐
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
May 15, 2026 at 2:39 PM
⭐ #NARBreakthrough! ⭐
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
Cleavage of the RNA polymerase II general transcription factor TFIIB tunes transcription during stress
Glaunsinger, B., Gulyas, L. et al.
Paper
Details
#RNApolII #TFIIB #TranscriptionRegulation
Glaunsinger, B., Gulyas, L. et al.
Paper
Details
#RNApolII #TFIIB #TranscriptionRegulation
July 29, 2025 at 4:03 PM
Cleavage of the RNA polymerase II general transcription factor TFIIB tunes transcription during stress
Glaunsinger, B., Gulyas, L. et al.
Paper
Details
#RNApolII #TFIIB #TranscriptionRegulation
Glaunsinger, B., Gulyas, L. et al.
Paper
Details
#RNApolII #TFIIB #TranscriptionRegulation
Our PLAMseq paper is out today in @ScienceAdvances . We developed a TurboID-based protein-genomic approach to map proteins and protein interactions in the genome. Validated with CTCF and RNApolII. Applied to map Histone H1 SUMOylation genomic loci.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single workflow
PLAMseq enables performance of genomics and proteomics of chromatin-associated proteins and protein interactions simultaneously.
www.science.org
November 7, 2025 at 9:25 PM
Our PLAMseq paper is out today in @ScienceAdvances . We developed a TurboID-based protein-genomic approach to map proteins and protein interactions in the genome. Validated with CTCF and RNApolII. Applied to map Histone H1 SUMOylation genomic loci.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...