ICYMI: New online: Structural insights into transcriptional regulation by the helicase RECQL5
Structural insights into transcriptional regulation by the helicase RECQL5
Nature Structural & Molecular Biology, Published online: 07 July 2025; doi:10.1038/s41594-025-01611-8Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s translocation state, potentially restarting stalled transcription.
go.nature.com
July 8, 2025 at 9:15 AM
ICYMI: New online: Structural insights into transcriptional regulation by the helicase RECQL5
ICYMI: New online: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
Nature Structural & Molecular Biology, Published online: 07 July 2025; doi:10.1038/s41594-025-01586-6Using cryo-electron microscopy and biochemistry, Zhang et al. reveal that the DNA helicase RECQL5 and the transcription-coupled DNA repair complex coordinate to regulate transcription elongation rates and maintain genome stability.
go.nature.com
July 8, 2025 at 9:15 AM
ICYMI: New online: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
New online: Structural insights into transcriptional regulation by the helicase RECQL5
Structural insights into transcriptional regulation by the helicase RECQL5
Nature Structural & Molecular Biology, Published online: 07 July 2025; doi:10.1038/s41594-025-01611-8Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s translocation state, potentially restarting stalled transcription.
go.nature.com
July 7, 2025 at 9:14 AM
New online: Structural insights into transcriptional regulation by the helicase RECQL5
New online: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
Nature Structural & Molecular Biology, Published online: 07 July 2025; doi:10.1038/s41594-025-01586-6Using cryo-electron microscopy and biochemistry, Zhang et al. reveal that the DNA helicase RECQL5 and the transcription-coupled DNA repair complex coordinate to regulate transcription elongation rates and maintain genome stability.
go.nature.com
July 7, 2025 at 9:14 AM
New online: Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation
Data Processing for this project was beyond conventional and I came out with some strategies to solve the large local heterogeneity for RECQL5. I hope you all enjoy! (2/2)
January 31, 2025 at 4:26 AM
Data Processing for this project was beyond conventional and I came out with some strategies to solve the large local heterogeneity for RECQL5. I hope you all enjoy! (2/2)
Come to read our pre-print on Transcription Regulation by RECQL5 helicase! This work marks my 1st First-author paper as a PhD student. I have the pleasure of co-lead this project with the excellent postdoc and great lab mate @nlue8.bsky.social (1/2)
Check out our lab’s latest work on the regulation of transcription by RECQL5 helicase! This work was co-led by @alfredo0712.bsky.social and @nlue8.bsky.social, with contributions from Patricia Grob, Ben Kaeser, Jie Fang, and Susanne Kassube. 1/7
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Structural insights into transcriptional regulation by the helicase RECQL5
Transcription and its regulation pose a major challenge for genome stability. The helicase RECQL5 has been proposed as an important factor to help safeguard the genome, and is the only member of the h...
www.biorxiv.org
January 31, 2025 at 4:26 AM
Come to read our pre-print on Transcription Regulation by RECQL5 helicase! This work marks my 1st First-author paper as a PhD student. I have the pleasure of co-lead this project with the excellent postdoc and great lab mate @nlue8.bsky.social (1/2)
See also this nice piece of article created by the LMB news team on our work
www2.mrc-lmb.cam.ac.uk/how-recql5-a...
www2.mrc-lmb.cam.ac.uk/how-recql5-a...
July 7, 2025 at 2:00 PM
See also this nice piece of article created by the LMB news team on our work
www2.mrc-lmb.cam.ac.uk/how-recql5-a...
www2.mrc-lmb.cam.ac.uk/how-recql5-a...
A transcription balance required for genome stability 🧬⚖️
Our work on RNA Pol II transcription speed control by the DNA helicase RECQL5 and the transcription-coupled DNA repair complex is now on @natsmb.nature.com
#CryoEM #transcription #genomestability
www.nature.com/articles/s41...
Our work on RNA Pol II transcription speed control by the DNA helicase RECQL5 and the transcription-coupled DNA repair complex is now on @natsmb.nature.com
#CryoEM #transcription #genomestability
www.nature.com/articles/s41...
Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation - Nature Structural & Molecular Biology
Using cryo-electron microscopy and biochemistry, Zhang et al. reveal that the DNA helicase RECQL5 and the transcription-coupled DNA repair complex coordinate to regulate transcription elongation rates...
www.nature.com
July 7, 2025 at 2:00 PM
A transcription balance required for genome stability 🧬⚖️
Our work on RNA Pol II transcription speed control by the DNA helicase RECQL5 and the transcription-coupled DNA repair complex is now on @natsmb.nature.com
#CryoEM #transcription #genomestability
www.nature.com/articles/s41...
Our work on RNA Pol II transcription speed control by the DNA helicase RECQL5 and the transcription-coupled DNA repair complex is now on @natsmb.nature.com
#CryoEM #transcription #genomestability
www.nature.com/articles/s41...
See also the beautiful work from the @nogaleslab.bsky.social on RECQL5 www.biorxiv.org/content/10.1...
February 3, 2025 at 12:23 PM
See also the beautiful work from the @nogaleslab.bsky.social on RECQL5 www.biorxiv.org/content/10.1...
Ever wondered how Pol II speed is controlled during productive elongation?
Our study reveals that the helicase RECQL5 triggers transcription braking, while the transcription-coupled DNA repair complex restores the elongation rate. #cryoEM #transcription
www.biorxiv.org/content/10.1...
Our study reveals that the helicase RECQL5 triggers transcription braking, while the transcription-coupled DNA repair complex restores the elongation rate. #cryoEM #transcription
www.biorxiv.org/content/10.1...
February 3, 2025 at 12:23 PM
Ever wondered how Pol II speed is controlled during productive elongation?
Our study reveals that the helicase RECQL5 triggers transcription braking, while the transcription-coupled DNA repair complex restores the elongation rate. #cryoEM #transcription
www.biorxiv.org/content/10.1...
Our study reveals that the helicase RECQL5 triggers transcription braking, while the transcription-coupled DNA repair complex restores the elongation rate. #cryoEM #transcription
www.biorxiv.org/content/10.1...
Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work.
www.nature.com/articles/s41...
Structural insights into transcriptional regulation by the helicase RECQL5 - Nature Structural & Molecular Biology
Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s ...
www.nature.com
July 7, 2025 at 10:35 AM
Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work.
www.nature.com/articles/s41...
Beyond a role in slowing down Pol II elongation, RECQL5 is known to decrease transcription stress. We propose that RECQL5 may help restart transcription from stalled Pol II by allosterically modulating Pol II’s translocation state. 6/7
January 31, 2025 at 3:24 AM
Beyond a role in slowing down Pol II elongation, RECQL5 is known to decrease transcription stress. We propose that RECQL5 may help restart transcription from stalled Pol II by allosterically modulating Pol II’s translocation state. 6/7
Nucleotide binding (AMPPNP or ADP) causes the RECQL5 helicase domain to undergo a conformational change, pulling on the DNA to induce Pol II to shift to a post-translocation state. Meanwhile, the IRI module seems to serve as a stable anchor for RECQL5 binding. 5/7
January 31, 2025 at 3:24 AM
Nucleotide binding (AMPPNP or ADP) causes the RECQL5 helicase domain to undergo a conformational change, pulling on the DNA to induce Pol II to shift to a post-translocation state. Meanwhile, the IRI module seems to serve as a stable anchor for RECQL5 binding. 5/7
The high resolution of our structures also enabled us to ask how RECQL5 affects Pol II, in particular its translocation state on a DNA/RNA scaffold. We found that Pol II occupies different translocation states when RECQL5 helicase is in its apo vs. nucleotide-bound form. 4/7
January 31, 2025 at 3:24 AM
The high resolution of our structures also enabled us to ask how RECQL5 affects Pol II, in particular its translocation state on a DNA/RNA scaffold. We found that Pol II occupies different translocation states when RECQL5 helicase is in its apo vs. nucleotide-bound form. 4/7
We report high-resolution structures of Pol II elongation complexes with RECQL5, visualizing interactions between RECQL5, Pol II, and DNA, confirming its role as a transcriptional roadblock. This required extensive data processing, as RECQL5 is very flexible! 3/7
January 31, 2025 at 3:24 AM
We report high-resolution structures of Pol II elongation complexes with RECQL5, visualizing interactions between RECQL5, Pol II, and DNA, confirming its role as a transcriptional roadblock. This required extensive data processing, as RECQL5 is very flexible! 3/7
RecQ helicases help safeguard genome stability. In this family, RECQL5 stands out as the only one to interact with and inhibit RNA Pol II, and it’s been shown to help decrease transcription stress. We used cryo-EM to investigate the molecular basis for this mechanism. 2/7
January 31, 2025 at 3:24 AM
RecQ helicases help safeguard genome stability. In this family, RECQL5 stands out as the only one to interact with and inhibit RNA Pol II, and it’s been shown to help decrease transcription stress. We used cryo-EM to investigate the molecular basis for this mechanism. 2/7
Check out our lab’s latest work on the regulation of transcription by RECQL5 helicase! This work was co-led by @alfredo0712.bsky.social and @nlue8.bsky.social, with contributions from Patricia Grob, Ben Kaeser, Jie Fang, and Susanne Kassube. 1/7
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Structural insights into transcriptional regulation by the helicase RECQL5
Transcription and its regulation pose a major challenge for genome stability. The helicase RECQL5 has been proposed as an important factor to help safeguard the genome, and is the only member of the h...
www.biorxiv.org
January 31, 2025 at 3:24 AM
Check out our lab’s latest work on the regulation of transcription by RECQL5 helicase! This work was co-led by @alfredo0712.bsky.social and @nlue8.bsky.social, with contributions from Patricia Grob, Ben Kaeser, Jie Fang, and Susanne Kassube. 1/7
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Study links Lynch-like syndrome to DNA repair defects, identifying RecQ helicase variants as contributors to #colorectalcancer risk and supporting expanded genetic testing panels. bit.ly/4wDSgaw #GIMO #RECQL5 #DNAHelicases #GermlineVariants #LynchLikeSyndrome
May 19, 2026 at 1:40 AM
Study links Lynch-like syndrome to DNA repair defects, identifying RecQ helicase variants as contributors to #colorectalcancer risk and supporting expanded genetic testing panels. bit.ly/4wDSgaw #GIMO #RECQL5 #DNAHelicases #GermlineVariants #LynchLikeSyndrome
Nice new structure: RecQL5 prevents transcription elongation at DNA damage by 2 mechanisms. http://goo.gl/RZffI
Browse Articles
| Nature Structural & Molecular B...
Browse the archive of articles on Nature Structural & Mol...
goo.gl
November 24, 2024 at 6:31 AM
Nice new structure: RecQL5 prevents transcription elongation at DNA damage by 2 mechanisms. http://goo.gl/RZffI
Structural insights into transcriptional regulation by the helicase RECQL5
->Nature | More info from EcoSearch
->Nature | More info from EcoSearch
Structural insights into transcriptional regulation by the helicase RECQL5
GST-tagged RECQL5 1–620 was expressed in E. coli BL21-CodonPlus (DE3)-RIL cells (Stratagene) for 16 h at 18 °C and the cells were lysed with a cell disrupter (Avestin)22. The clarified lysate was loaded onto a glutathione Sepharose 4 fast-flow column...
www.nature.com
July 8, 2025 at 12:16 AM
Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation pubmed.ncbi.nlm.nih.gov/40624163/ #cryoem
July 8, 2025 at 8:19 PM
Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation pubmed.ncbi.nlm.nih.gov/40624163/ #cryoem
Structural insights into transcriptional regulation by the helicase RECQL5 pubmed.ncbi.nlm.nih.gov/40624164/ #cryoem
July 8, 2025 at 6:40 PM
Structural insights into transcriptional regulation by the helicase RECQL5 pubmed.ncbi.nlm.nih.gov/40624164/ #cryoem
Structural insights into transcriptional regulation by the helicase RECQL5 www.biorxiv.org/content/10.1101/2025.01.29.634372v1 #cryoem
January 30, 2025 at 1:06 PM
Structural insights into transcriptional regulation by the helicase RECQL5 www.biorxiv.org/content/10.1101/2025.01.29.634372v1 #cryoem
⭐ #NARBreakthrough! ⭐
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
May 15, 2026 at 2:39 PM
⭐ #NARBreakthrough! ⭐
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
New #cryo-EM study reveals how #transcription factor IWS1 promotes #RNApolII #elongation. Its C-terminus contains #modularmotifs that bind elongation components, stabilizing the complex. IWS1 also competes with #RECQL5 for Pol II binding.
📖 Read here: doi.org/10.1093/nar/...
Spearheaded by PhD student Luojia Zhang, @suyangzhang.bsky.social’s group have identified a model mechanism for regulating transcription elongation speed, balancing efficient gene expression and genome stability.
Read more: www2.mrc-lmb.cam.ac.uk/how-recql5-a...
#LMBResearch #cryoEM 🧪
Read more: www2.mrc-lmb.cam.ac.uk/how-recql5-a...
#LMBResearch #cryoEM 🧪
July 7, 2025 at 11:59 AM
Spearheaded by PhD student Luojia Zhang, @suyangzhang.bsky.social’s group have identified a model mechanism for regulating transcription elongation speed, balancing efficient gene expression and genome stability.
Read more: www2.mrc-lmb.cam.ac.uk/how-recql5-a...
#LMBResearch #cryoEM 🧪
Read more: www2.mrc-lmb.cam.ac.uk/how-recql5-a...
#LMBResearch #cryoEM 🧪