Proximal Ascending aorta is made of #SmoothMuscleCell of dual lineage origins
Luminal side: Wnt1+ #CardiacNeuralCrest
Abluminal side: Mef2c+ #SecondHeartField

Their transcript profiles by scRNAseq + snATACseq👇

#AortaEd
#ATVB 2024 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

Regionalized & Sex-dependent scRNAseq of 🐭Aorta

Carotid
Aortic Arch
Descending Thoracic
Abdominal Aortic #SmoothMuscleCell

Arch
#SecondHeartField vs #NeuralCrest SMC

339 Regional SMC mRNA features ➡️ cardiovascular trait GWAS

#AortaEd
#NatCardiovascRes 2025
www.nature.com/articles/s44...

Sox10+ #NeuralCrest SMAD4 KO e9.5🐭abolishes
NC-derived #SmoothMuscleCell investment in remod #AorticArchArteries

BUT lack of NC-SMC doesn't⏬aortic arch SMC coverage-> Compensated by #SecondHeartField SMCs?🤓

@sophie_astrof @DevelopmentalDy 2023
anatomypubs.onlinelibrary.wiley.com/doi/10.1002/...

ADAMTS16 as a causal gene for #BiscupidAorticValve
Targeted Exon seq 304 BAV+301 Ctrl➡️p.H357Q

🐭Adamts16 H355Q/+ in both Tie2+ #EndothelialCell & Isl1+ #SecondHeartField, not Wnt1+ #NeuralCrest, contribute to developmental raphe formation

#CircAHA 2023
www.ahajournals.org/doi/abs/10.1...

#SecondHeartField #OutFlowTract

e7.5-9.5 APJ, Apelin & Elabela receptor, is SHF-enriched
e10.5 APJ turns vascular-specific

e7.5 APJ+ cell progenies contribute to both #PulmonaryTrunk+#Aorta

e8.5 they prefer PT
Dr. Bikram Sharma lab Dev Biol 2023
www.sciencedirect.com/science/arti...