#T4SS
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vectorgen.bsky.social
Anyone interested in some Wolbachia T4SS goodness? And other weird stuff?

Started years ago by @bigvirushunter.bsky.social in my lab, pulled over finish line by awesome collaboration w Matt Swulius, Carrie Shaffer, & Mohammed Kaplan (don't tthink they're on bsky)

www.biorxiv.org/content/10.1...
August 30, 2025 at 12:37 PM
cascaleslab.bsky.social
T2SS ? T4SS ? CDI ? T6SS ? T7SS ?
NO ! The T9SS is also involved in interbacterial competition !!

Great work by Maelle Paillat @maellepllt.bsky.social under the supervision of Thierry Doan @thicoz.bsky.social Congratulations to both ! Glad to see this story out !
May 8, 2026 at 8:55 PM
figlegend.bsky.social
Here is it! Super new science from us on horizontal gene transfer & bact defense systems! Liyana OW YONG discovered the first-of-its-kind defense factor AbjA that triggers 'abortive conjugation' as a defense mechanism, by targeting the T4SS! How neat?! #MicroSky 1/7

www.biorxiv.org/content/10.6...
March 23, 2026 at 1:42 PM
ulikluemper.bsky.social
I had great fun presenting our work on „Impact of T4SS on the environmental spread of plasmids and antimicrobial resistance“ in a keynote lecture at the international T4SS conference in Nancy.

Thanks to the organisers for the invite and the fancy conference gala dinner in Nancy City Hall.

#AMR
February 21, 2025 at 11:46 AM
debnathghosal.bsky.social
As a postdoc at Caltech, I attended a conference in Germany on the Bacterial Type IV Secretion System(T4SS). The T4SS is a versatile molecular weaponry used by bacteria to cause several diseases and spread AMR. I presented the first complete structure of the T4SS and won the best presentation award.
March 16, 2025 at 12:34 PM
badreddinedouzi.bsky.social
Happy to share a new review proposing a unified nomenclature for T4SSs.
Great collaboration with Peter J Christie, Gabriel Waksman, Ronnie Per-Arne Berntsson and our team.
academic.oup.com/femsre/artic...
#T4SS#Microbiology
Type IV secretion systems: reconciling diversity through a unified nomenclature
This review presents a comprehensive overview of Type IV Secretion Systems (T4SSs), focusing on their structural, functional, and evolutionary diversity ac
academic.oup.com
February 18, 2026 at 2:13 PM
badreddinedouzi.bsky.social
Exited to start the T4SS conference.

www.t4ss-conference-nancy.org
Home - T4SS
www.t4ss-conference-nancy.org
February 17, 2025 at 11:06 AM
ritaramalhete.bsky.social
Did you know that the #T4SS can also mediate biofilm formation?🧫 A #Crohnsdisease associated E. coli strain carries a hybrid T4SS that drives pilin polymerization and #biofilm formation, leading to enhanced fitness in the gut. Read about it here ⬇️ @natcomms.nature.com #naturecommunications
October 9, 2025 at 9:52 AM
okagu.bsky.social
Happy to share our new bioRxiv preprint:

“To Kill or not to Kill: A Conserved Trans-Intoxication Protection Factor Blocks X-T4SS-Mediated Fratricide through Interaction with VirB5”

www.biorxiv.org/content/10.6...
May 11, 2026 at 12:10 PM
femsjournals.bsky.social
🦠 @laurentterradot.bsky.social lab reviews #Brucella’s #T4SS—a key virulence factor—covering its structure, function, and growing effector arsenal. It also explores how effectors enter host cells, opening new paths to understand and target infection. 🔬

👉 buff.ly/KW4kVN4
January 26, 2026 at 8:01 AM
femsjournals.bsky.social
TraF from a broad-host monoderm plasmid is the first conjugative T4SS component with strong homology to T7SS pseudokinase 🧬 Grohmann & Keller labs elucidate its crystal structure, function and partners. @uni-grat.at & BHT_berliner

👉️ buff.ly/ndoiPD4
May 3, 2026 at 10:01 AM
tlowepower.bsky.social
First, first-author publication from undergrad Tabitha Cowell is live!

She did some very cool comparisons of putative conjugative T4SS gene clusters and found neat eco evolutionary differences between the wilt pathogen and more environmental species!

I'll make an explainer sometime this week.
biorxiv-microbiol.bsky.social bioRxiv Microbiology @biorxiv-microbiol.bsky.social · Sep 28
Lifestyle-associated variation in type IV secretion systems between phytopathogenic and environmental Ralstonia https://www.biorxiv.org/content/10.1101/2025.09.26.675681v1
September 29, 2025 at 12:42 AM
lowlab.bsky.social
In-situ SPA 🧨

www.biorxiv.org/content/10.1...
In-situ structures of the Legionella Dot/Icm T4SS identify the DotA-IcmX complex as the gatekeeper for effector translocation
The Dot/Icm machine in Legionella pneumophila is one of the most versatile type IV secretion systems (T4SSs), with a remarkable capacity to translocate over 330 different effector proteins across the ...
www.biorxiv.org
June 30, 2025 at 8:16 PM
sbigot.bsky.social
New preprint from our Lesterlin Lab! Yannick Baffert and @nfrk92.bsky.social, in collaboration with @macerwan.bsky.social and @val-meve.bsky.social, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!
Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli
Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmi...
www.biorxiv.org
January 25, 2025 at 9:20 AM
josyterbeek.bsky.social
📣New preprint with @rberntsson.bsky.social👇We overturn the long-standing view that Gram-positive bacteria lack conjugative pili! We identify pili-forming proteins in a wide range of bacteria and show they're essential for the spread of #antibioticresistance #AMR 🧵1/6
www.biorxiv.org/content/10.6...
Pili are essential for conjugation also in many Gram-positive bacteria
Type IV secretion systems (T4SS) enable the spread of antibiotic resistance and other virulence factors. In Gram-positive bacteria, T4SSs have long been thought to lack VirB2-like proteins that form c...
www.biorxiv.org
December 30, 2025 at 6:03 PM
doradomoralesp.bsky.social
A plasmid and an ICE teaming up... 🤯😱

www.nature.com/articles/s41...
The assembly of a hybrid type IV secretion system by a Crohn’s disease-associated Escherichia coli strain - Nature Communications
Adherent-invasive strains of E. coli are commonly isolated from patients with Crohn’s disease. Here, the authors show that an AIEC harbours a hybrid Type IV secretion system (T4SS) that mediates pilin polymerization and biofilm formation in vivo.
www.nature.com
October 3, 2025 at 2:17 PM
cryoempapers.bsky.social
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS pubmed.ncbi.nlm.nih.gov/41510630/ #cryoem
January 11, 2026 at 5:24 AM
stcmicrobeblog.bsky.social
#PhageSky #ProtistsOnSky

endosymbionts have "their" phages, too. cyanos of sponges (below), 𝘞𝘰𝘭𝘣𝘢𝘤𝘩𝘪𝘢 of insects, and then we have the piñata 👇

schaechter.asmblog.org/schaechter/2...
Putative interactions between the MAnky­pha­ge, two bacterial endosymbionts (M. polyxenophila and G. numerosa), and the cryptomonad host. C. gyro­py­renoidosa harbors 4 genomes (in green): nuclear, mi­tochondrial (MT), nucleomorph (NM), and plastid. Mo­bile elements are found in the endosymbionts' chromo­somes and plasmids, particularly in M. polyxenophila. Only G. numerosa encodes a flagellum, but both endosymbionts have type IV secretion systems (T4SS) and ATP/ADP translocases. The endosymbionts and phage also encode eukaryotic-like protein domains like ankyrin repeats (ANKs) and leucine-rich repeats (LRRs). Other putative eukaryotic interactions include the MAnkyphage-encoded protein (DUF3685) with homo­logy to response regulators in plastids and cyanobac­teria. MAnkyphage harbors a type II toxin‑antitoxin system present in M. polyxenophila. The tally of gen­omes in this single-celled cryptomonad comes to 7 plus 3 bacterial plasmids. Source: https://pubmed.ncbi.nlm.nih.gov/37116483/
July 28, 2025 at 9:04 PM
waelelhenawy.bsky.social
Happy to share our latest pub in @natcomms.nature.com led by my trainee Jonas Wong www.nature.com/articles/s41.... We identified an 'unusual' T4SS used by Crohn's E. coli to make biofilms and ⬆️ inflammation. We're hiring a PDF to continue this. Details: iaejup.fa.ocs.oraclecloud.com/hcmUI/Candid...
The assembly of a hybrid type IV secretion system by a Crohn’s disease-associated Escherichia coli strain - Nature Communications
Adherent-invasive strains of E. coli are commonly isolated from patients with Crohn’s disease. Here, the authors show that an AIEC harbours a hybrid Type IV secretion system (T4SS) that mediates pilin polymerization and biofilm formation in vivo.
www.nature.com
October 7, 2025 at 5:00 PM
biorxiv-microbiol.bsky.social
To Kill or not to Kill: A Conserved trans-intoxication protection factor Blocks X-T4SS-Mediated Fratricide through Interaction with VirB5 https://www.biorxiv.org/content/10.64898/2026.05.09.723464v1
May 11, 2026 at 4:16 AM
journals.asm.org/doi/10.1128/...
An Anaplasma phagocytophilum T4SS effector, AteA, is essential for tick infection | mBio
Ticks are the number one vector of pathogens for livestock worldwide and for humans in the United States. The biology of tick transmission is an understudied area. Understanding this critical interact...
journals.asm.org
September 29, 2023 at 4:59 AM
tlowepower.bsky.social
Tabitha is currently writing two first author publications from her undergrad work. One on development of a chemical competence protocol for our non-model bacterium and the other a rigorous genomics survey of T4SS gene clusters in our genus of interest.
March 15, 2025 at 3:06 PM
euroxanth.eu
Very happy to announce our paper @natecoevo.nature.com on the functional replacement of ancestral antibacterial secretion system ( #T4SS #T6SS ) in a bacterial plant pathogen ( #Xanthomonas ). Enjoy reading! rdcu.be/euNrM
Special thanks to @costprogramme.bsky.social !
Functional replacement of ancestral antibacterial secretion system in a bacterial plant pathogen
Nature Ecology & Evolution - The bacterial plant pathogen genus Xanthomonas uses two distinct secretion systems for antibacterial competition. Here the authors show that some Xanthomonas...
rdcu.be
July 7, 2025 at 1:33 PM
josyterbeek.bsky.social
we tried to fill this gap in knowledge, by:
1) identifying conserved proteins by #ProteinFolding & matching #FoldSeek
2) review their roles & #ProteinStructure #AlphaFold3
3) Propose a model for the G+ T4SS from #Enterococcus
Check out our work to learn more!
#StructuralBioinformatics #AIinScience
authors.elsevier.com
January 16, 2025 at 8:46 AM
evolvedbiofilm.bsky.social
Crucial Gram-positive Type IV Secretion System protein TraF is a structural homolog of Type VII Secretion System protein EssB/YukC

in #microLife @femsjournals.bsky.social

academic.oup.com/microlife/ad...
Crucial Gram-positive Type IV Secretion System protein TraF is a structural homolog of Type VII Secretion System protein EssB/YukC
Abstract. Type IV secretion systems (T4SS) are found in both monoderm and diderm bacteria. The broad-host-range conjugative plasmid pIP501 from Enterococcu
academic.oup.com
March 27, 2026 at 8:01 PM