WNT5A forms a gradient across the growth plate. Loss of GPC6 collapses it — WNT5A is consumed at its source, hyperactivating local signalling while depriving distal tissue of ligand! The same tissue experiences both gain and loss of signalling 🤯 GPC6 is a spatial allocator of WNT5A. #WNT5A #GPC6
September 22, 2026 at 2:42 PM
Source-proximal WNT5A hyperactivation has a completely unexpected consequence — it engages a previously unrecognised WNT5A–RAC–PAK–MEK–ERK cascade, driving pathological ERK activation in developing bone without any mutation in the conventional RAS–MAPK pathway. #RASopathy #ERK #WNT5A
September 22, 2026 at 2:42 PM
Source-proximal WNT5A hyperactivation has a completely unexpected consequence — it engages a previously unrecognised WNT5A–RAC–PAK–MEK–ERK cascade, driving pathological ERK activation in developing bone without any mutation in the conventional RAS–MAPK pathway. #RASopathy #ERK #WNT5A
Kevin Fang, Steffen Scholpp @scholpplab.bsky.social @lsiexeter.bsky.social and colleagues find that amyloid-β can activate JNK signalling via WNT5A-ROR2 to reduce synapse formation in Alzheimer's disease.
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
February 5, 2025 at 10:34 AM
Kevin Fang, Steffen Scholpp @scholpplab.bsky.social @lsiexeter.bsky.social and colleagues find that amyloid-β can activate JNK signalling via WNT5A-ROR2 to reduce synapse formation in Alzheimer's disease.
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
journals.biologists.com/jcs/article/...
#OpenAccess #ReadandPublish
#PsoriasisVulgaris #AutoimmuneDisease #SpatialTranscriptomics StereoSeq
A fibro-angiogenic niche in psoriatic👤skin epidermis
WNT5A+ fibroblast
VCAN+ #SmoothMuscleCell
ITIH5+ #EndothelialCell
Fibroblast, SMC & Schwann cell are major signal senders
#BrJDermatol 2024
academic.oup.com/bjd/advance-...
A fibro-angiogenic niche in psoriatic👤skin epidermis
WNT5A+ fibroblast
VCAN+ #SmoothMuscleCell
ITIH5+ #EndothelialCell
Fibroblast, SMC & Schwann cell are major signal senders
#BrJDermatol 2024
academic.oup.com/bjd/advance-...
December 15, 2024 at 1:08 PM
#PsoriasisVulgaris #AutoimmuneDisease #SpatialTranscriptomics StereoSeq
A fibro-angiogenic niche in psoriatic👤skin epidermis
WNT5A+ fibroblast
VCAN+ #SmoothMuscleCell
ITIH5+ #EndothelialCell
Fibroblast, SMC & Schwann cell are major signal senders
#BrJDermatol 2024
academic.oup.com/bjd/advance-...
A fibro-angiogenic niche in psoriatic👤skin epidermis
WNT5A+ fibroblast
VCAN+ #SmoothMuscleCell
ITIH5+ #EndothelialCell
Fibroblast, SMC & Schwann cell are major signal senders
#BrJDermatol 2024
academic.oup.com/bjd/advance-...
Study shows Wnt5a, from fibroblasts near tumor lumens, fuels colon cancer. Its depletion curbs growth. Meta-analysis identifies key fibroblast subtypes. PMID:40246836, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-58748-9 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
Hypoxia-induced Wnt5a-secreting fibroblasts promote colon cancer progression | Nature Communications
Wnt5a, a representative Wnt ligand that activates the β-catenin-independent pathway, has been shown to promote tumorigenesis. However, it is unclear where Wnt5a is produced and how it affects colon cancer aggressiveness. In this study, we demonstrate that Wnt5a is expressed in fibroblasts near the luminal side of the tumor, and its depletion suppresses mouse colon cancer formation. To characterize the specific fibroblast subtype, a meta-analysis of human and mouse colon fibroblast single-cell RNA-seq data is performed. The results show that Wnt5a is expressed in hypoxia-induced inflammatory fibroblast (InfFib), accompanied by the activation of HIF2. Moreover, Wnt5a maintains InfFib through the suppression of angiogenesis mediated by soluble VEGF receptor1 (Flt1) secretion from endothelial cells, thereby inducing further hypoxia. InfFib also produces epiregulin, which promotes colon cancer growth. Here, we show that Wnt5a acts on endothelial cells, inducing a hypoxic environment that ma
doi.org
April 22, 2025 at 5:10 AM
Loss of GPC6 generates a molecular hybrid of 2 developmental diseases: WNT5A deficiency characteristic of Robinow syndrome and pathological ERK activation characteristic of the RASopathies. Omodysplasia caused by loss of GPC6 overlaps both disease classes. #omodysplasia #RASopathy #Robinow
September 22, 2026 at 2:42 PM
Loss of GPC6 generates a molecular hybrid of 2 developmental diseases: WNT5A deficiency characteristic of Robinow syndrome and pathological ERK activation characteristic of the RASopathies. Omodysplasia caused by loss of GPC6 overlaps both disease classes. #omodysplasia #RASopathy #Robinow
Proud to share the labs first preprint! Led by Yeon, whose hard work, dedication and drive have been instrumental in bringing the project to this point. Thank you to everyone involved, especially long-term collaborators Yvonne and Yuguang. #Wnt #WntUK #RASopathy #ERK
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State
Morphogen gradients require ligands to disperse across tissues from their sites of production, yet receptor engagement can consume ligand before it reaches distant cells. This poses a particular chall...
www.biorxiv.org
September 22, 2026 at 2:42 PM
Proud to share the labs first preprint! Led by Yeon, whose hard work, dedication and drive have been instrumental in bringing the project to this point. Thank you to everyone involved, especially long-term collaborators Yvonne and Yuguang. #Wnt #WntUK #RASopathy #ERK
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Loss of GPC6 drives pathological ERK activation in developing bone — without any mutation in the RAS–MAPK pathway. pERK signal (green) is markedly elevated in GPC6 KO compared to wildtype. A RASopathy-like state from a WNT gradient defect 🤯 #RASopathy #ERK #WNT5A"
September 22, 2026 at 2:42 PM
Loss of GPC6 drives pathological ERK activation in developing bone — without any mutation in the RAS–MAPK pathway. pERK signal (green) is markedly elevated in GPC6 KO compared to wildtype. A RASopathy-like state from a WNT gradient defect 🤯 #RASopathy #ERK #WNT5A"
Our PhD Talents! Congratulations 🥳 @josefbryja.bsky.social @loschmidtlab.bsky.social
March 24, 2025 at 6:31 PM
Our PhD Talents! Congratulations 🥳 @josefbryja.bsky.social @loschmidtlab.bsky.social
I'm delighted to see this beautiful study out. WNT5A hyperactivation drives the pathological ERK1/2 signalling. #RASopathies and implicaitons for cancer, what's not too like?!
Congratulations to Ian, Yeonjoo and all their collaborators! 👏 👏 👏 👏 👏
P.S. Take the rest of the day off!
Congratulations to Ian, Yeonjoo and all their collaborators! 👏 👏 👏 👏 👏
P.S. Take the rest of the day off!
Proud to share the labs first preprint! Led by Yeon, whose hard work, dedication and drive have been instrumental in bringing the project to this point. Thank you to everyone involved, especially long-term collaborators Yvonne and Yuguang. #Wnt #WntUK #RASopathy #ERK
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State
Morphogen gradients require ligands to disperse across tissues from their sites of production, yet receptor engagement can consume ligand before it reaches distant cells. This poses a particular chall...
www.biorxiv.org
September 22, 2026 at 4:48 PM
I'm delighted to see this beautiful study out. WNT5A hyperactivation drives the pathological ERK1/2 signalling. #RASopathies and implicaitons for cancer, what's not too like?!
Congratulations to Ian, Yeonjoo and all their collaborators! 👏 👏 👏 👏 👏
P.S. Take the rest of the day off!
Congratulations to Ian, Yeonjoo and all their collaborators! 👏 👏 👏 👏 👏
P.S. Take the rest of the day off!
GPC6 shields the WNT5A lipid from premature Frizzled engagement, providing a protected window for diffusion and preserving a tissue-scale WNT pool for long-range action. Rather than simply maintaining solubility, GPC6 controls where signalling-competent WNT5A is used. #WNT #morphogen #gradients
September 22, 2026 at 2:42 PM
GPC6 shields the WNT5A lipid from premature Frizzled engagement, providing a protected window for diffusion and preserving a tissue-scale WNT pool for long-range action. Rather than simply maintaining solubility, GPC6 controls where signalling-competent WNT5A is used. #WNT #morphogen #gradients
GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State https://www.biorxiv.org/content/10.64898/2026.09.20.752969v1
September 22, 2026 at 11:31 AM
GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State https://www.biorxiv.org/content/10.64898/2026.09.20.752969v1
Robo2 as a surface marker for FACS 👤dermal papilla cells
Robo2 is induced by CHIR99021 + FGF9
CH+F9 seems superior to BIO+BMP2+FGF2 & synergize with 3D aggregation culture in maintaining hDPC markers (WNT5A/LEF1/NOG/BMP4/VCAN/SPRY4/SOX2)
#ExpDermatol 2026
onlinelibrary.wiley.com/doi/10.1111/...
Robo2 is induced by CHIR99021 + FGF9
CH+F9 seems superior to BIO+BMP2+FGF2 & synergize with 3D aggregation culture in maintaining hDPC markers (WNT5A/LEF1/NOG/BMP4/VCAN/SPRY4/SOX2)
#ExpDermatol 2026
onlinelibrary.wiley.com/doi/10.1111/...
June 23, 2026 at 12:34 PM
Robo2 as a surface marker for FACS 👤dermal papilla cells
Robo2 is induced by CHIR99021 + FGF9
CH+F9 seems superior to BIO+BMP2+FGF2 & synergize with 3D aggregation culture in maintaining hDPC markers (WNT5A/LEF1/NOG/BMP4/VCAN/SPRY4/SOX2)
#ExpDermatol 2026
onlinelibrary.wiley.com/doi/10.1111/...
Robo2 is induced by CHIR99021 + FGF9
CH+F9 seems superior to BIO+BMP2+FGF2 & synergize with 3D aggregation culture in maintaining hDPC markers (WNT5A/LEF1/NOG/BMP4/VCAN/SPRY4/SOX2)
#ExpDermatol 2026
onlinelibrary.wiley.com/doi/10.1111/...
GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State https://www.biorxiv.org/content/10.64898/2026.09.20.752969v1
September 22, 2026 at 11:31 AM
GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State https://www.biorxiv.org/content/10.64898/2026.09.20.752969v1
1/1 Our recent research sheds light on how Alzheimer's disease interferes with communication between brain cells. We identified a specific pathway (Wnt/PCP-ROR2) contributing to synapse loss, a crucial element in cognitive decline. tinyurl.com/4vh2uk3x @jcellsci.bsky.social @lsiexeter.bsky.social
Amyloid-β can activate JNK signalling via WNT5A-ROR2 to reduce synapse formation in Alzheimer's disease
Summary: The non-canonical Wnt/PCP pathway, specifically the ROR2 receptor, has a role in Alzheimer's disease mediated by Aβ-induced synaptic dysfunction by activating JNK signalling.
journals.biologists.com
February 5, 2025 at 9:49 AM
1/1 Our recent research sheds light on how Alzheimer's disease interferes with communication between brain cells. We identified a specific pathway (Wnt/PCP-ROR2) contributing to synapse loss, a crucial element in cognitive decline. tinyurl.com/4vh2uk3x @jcellsci.bsky.social @lsiexeter.bsky.social
KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy
pubmed.ncbi.nlm.nih.gov/40204099/
pubmed.ncbi.nlm.nih.gov/40204099/
KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy - PubMed
PaperClip The adult pancreas protects against cancer by actively expelling genetically mutated cells. Pancreatic cancer starts with cells carrying KRAS mutations; however, it is not clear how some KRA...
pubmed.ncbi.nlm.nih.gov
April 14, 2025 at 11:01 AM
KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy
pubmed.ncbi.nlm.nih.gov/40204099/
pubmed.ncbi.nlm.nih.gov/40204099/
Time for a weekly #AskZebrafish roundup! If you have any leads on the following, please reach out (or sign back into Slack 😉)
Looking for:
-plasmid for Tol2-vglut2a: fluorescence protein
-wntless & wnt5a/b mutants in Europe
-UAS-driven photoconvertible reporter lines in Europe
-advice on JC10 dye
Looking for:
-plasmid for Tol2-vglut2a: fluorescence protein
-wntless & wnt5a/b mutants in Europe
-UAS-driven photoconvertible reporter lines in Europe
-advice on JC10 dye
April 10, 2026 at 12:03 PM
Time for a weekly #AskZebrafish roundup! If you have any leads on the following, please reach out (or sign back into Slack 😉)
Looking for:
-plasmid for Tol2-vglut2a: fluorescence protein
-wntless & wnt5a/b mutants in Europe
-UAS-driven photoconvertible reporter lines in Europe
-advice on JC10 dye
Looking for:
-plasmid for Tol2-vglut2a: fluorescence protein
-wntless & wnt5a/b mutants in Europe
-UAS-driven photoconvertible reporter lines in Europe
-advice on JC10 dye
Wnt5a gain- and loss-of-function present distinctly in craniofacial bone https://www.biorxiv.org/content/10.1101/2025.07.21.665966v1
July 22, 2025 at 6:32 PM
Wnt5a gain- and loss-of-function present distinctly in craniofacial bone https://www.biorxiv.org/content/10.1101/2025.07.21.665966v1
Query from Slack: Looking for wls (wntless) and/or wnt5a/wnt5b mutant #zebrafish lines in Europe? If you have these or know any leads, please reach out 🙏 #AskZebrafish
April 2, 2026 at 5:15 AM
Query from Slack: Looking for wls (wntless) and/or wnt5a/wnt5b mutant #zebrafish lines in Europe? If you have these or know any leads, please reach out 🙏 #AskZebrafish
Omodysplasia is a rare severe skeletal dysplasia caused by loss of GPC6 — characterised by limb shortening and craniofacial abnormalities. Until now its molecular basis has been unclear. We show it likely arises from collapse of WNT5A gradients and pathological ERK activation. #omodysplasia
September 22, 2026 at 2:50 PM
Omodysplasia is a rare severe skeletal dysplasia caused by loss of GPC6 — characterised by limb shortening and craniofacial abnormalities. Until now its molecular basis has been unclear. We show it likely arises from collapse of WNT5A gradients and pathological ERK activation. #omodysplasia
The authors included RRIDs in their paper! Thanks for making your methods matter! #ReproducibleResearch #STMpublishing #BetterScience
Wnt5a regulates the expression of developmental genes in the adult retina following optic nerve crush injury
Read the full paper: Wnt5a regulates the expression of developmental genes in the adult retina following optic nerve crush injury
doi.org
March 19, 2026 at 7:04 AM
The authors included RRIDs in their paper! Thanks for making your methods matter! #ReproducibleResearch #STMpublishing #BetterScience
RRIDs were included in this in Advanced Science paper. We value the author's support of reproducibility. #OpenResearch #OpenScience #BetterScience
Targeting WTAP/ROR1/WNT5A‐Mediated Crosstalk Between Glioma Stem Cells and Macrophages to Normalize Tumor Vasculature and Enhance Chemotherapy
Read the full paper: Targeting WTAP/ROR1/WNT5A‐Mediated Crosstalk Between Glioma Stem Cells and Macrophages to Normalize Tumor Vasculature and Enhance Chemotherapy
doi.org
April 16, 2026 at 7:11 AM
RRIDs were included in this in Advanced Science paper. We value the author's support of reproducibility. #OpenResearch #OpenScience #BetterScience
Stromal SRD5A2 promotes prostate growth through WNT5A-LEF1-IGF1 signaling in benign prostatic hyperplasia.
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
December 15, 2025 at 2:53 PM
Stromal SRD5A2 promotes prostate growth through WNT5A-LEF1-IGF1 signaling in benign prostatic hyperplasia.
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
#ArticleinPress: Stromal SRD5A2 promotes prostate growth through WNT5A-LEF1-IGF1 signaling in benign prostatic hyperplasia.
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
September 24, 2025 at 7:03 PM
#ArticleinPress: Stromal SRD5A2 promotes prostate growth through WNT5A-LEF1-IGF1 signaling in benign prostatic hyperplasia.
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext
Full text: https://ajp.amjpathol.org/article/S0002-9440(25)00338-4/fulltext