Tzu-Jing Yang
@tzujingyang.bsky.social
Membrane protein biogenesis; MPI of Biophysics
Reposted by Tzu-Jing Yang
MemBrain v2 is on @natmethods.nature.com now? I never had a @biorxivpreprint.bsky.social with 164 citations before😅 ...Which is also to say: Thank you to the amazing #TeamTomo community, many of whom not only beta tested, but also contributed diverse training data. This one belongs to all of us! 🤗🔬🧪
September 8, 2026 at 11:10 AM
MemBrain v2 is on @natmethods.nature.com now? I never had a @biorxivpreprint.bsky.social with 164 citations before😅 ...Which is also to say: Thank you to the amazing #TeamTomo community, many of whom not only beta tested, but also contributed diverse training data. This one belongs to all of us! 🤗🔬🧪
Reposted by Tzu-Jing Yang
Check out our preprint on the structural mechanisms of how LEM2 and ESCRTs seal the nuclear envelope. Awesome collaboration with @karenpalacior.bsky.social who brought my structures to life 💃🔬❄️🌀 @hummerlab.bsky.social
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
September 4, 2026 at 9:45 AM
Check out our preprint on the structural mechanisms of how LEM2 and ESCRTs seal the nuclear envelope. Awesome collaboration with @karenpalacior.bsky.social who brought my structures to life 💃🔬❄️🌀 @hummerlab.bsky.social
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Tzu-Jing Yang
Identifying a broad-spectrum influenza inhibitor that blocks multiple functions of viral NS1 protein
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Identifying a broad-spectrum influenza inhibitor that blocks multiple functions of viral NS1 protein - Nature Communications
This study identifies a broad-spectrum IAV inhibitor that blocks multiple functions of NS1 protein. The critical sites on NS1 targeted by the inhibitor are validated in vitro and in vivo, supporting t...
www.nature.com
September 4, 2026 at 5:52 PM
Identifying a broad-spectrum influenza inhibitor that blocks multiple functions of viral NS1 protein
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tzu-Jing Yang
We are on air! Congratulations to everyone behind the story ❤️🔥 and thanks to @embojournal.org for providing the stage!
link.springer.com/article/10.1...
link.springer.com/article/10.1...
Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC - The EMBO Journal
The Sec translocon and the YidC/Oxa1-type insertases universally mediate biogenesis of α-helical membrane proteins, but the molecular basis of their cooperation has remained disputed. Recent discovery...
link.springer.com
June 26, 2026 at 9:13 AM
We are on air! Congratulations to everyone behind the story ❤️🔥 and thanks to @embojournal.org for providing the stage!
link.springer.com/article/10.1...
link.springer.com/article/10.1...
Reposted by Tzu-Jing Yang
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
May 27, 2026 at 10:14 AM
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
Reposted by Tzu-Jing Yang
Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
May 25, 2026 at 12:20 PM
Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs
doi.org/10.1038/s415...
Reposted by Tzu-Jing Yang
The first cut is the deepest: Cleavable signal peptides direct thousands of different nascent proteins to the endoplasmic reticulum. Oddly, SPs are all different and nevertheless cleaved with exquisite specificity. Our new cryo-EM structures + MD solve this paradox:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural basis of signal peptide recognition by the signal peptidase complex - Nature Communications
Signal peptides are highly variable, yet the signal peptidase complex processes them with remarkable specificity. Here, authors combine cryo-EM, molecular dynamics simulations and modeling to reveal a...
www.nature.com
May 25, 2026 at 10:03 PM
The first cut is the deepest: Cleavable signal peptides direct thousands of different nascent proteins to the endoplasmic reticulum. Oddly, SPs are all different and nevertheless cleaved with exquisite specificity. Our new cryo-EM structures + MD solve this paradox:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tzu-Jing Yang
Thrilled to share the updated video of my paper in Nature with Maciej (under supervision of @simonemattei.bsky.social & @jomaalab.bsky.social) Proud to use Cryo-ET to uncover how hibernation factors are key to translation restart! To model PTMs in situ and render animations fulfilled my PhD dream!🥹🎉
May 14, 2026 at 8:12 AM
Thrilled to share the updated video of my paper in Nature with Maciej (under supervision of @simonemattei.bsky.social & @jomaalab.bsky.social) Proud to use Cryo-ET to uncover how hibernation factors are key to translation restart! To model PTMs in situ and render animations fulfilled my PhD dream!🥹🎉
Reposted by Tzu-Jing Yang
We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better.
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
May 3, 2026 at 6:11 AM
We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better.
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
Reposted by Tzu-Jing Yang
Out now in Nature Communications!
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
April 21, 2026 at 4:25 PM
Out now in Nature Communications!
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
Reposted by Tzu-Jing Yang
Combining proteomics, structure prediction, bioinformatics and cryoET to discover non-canonical bacterial protein translocation-folding complex doi.org/10.1101/2025...
March 27, 2026 at 12:22 PM
Combining proteomics, structure prediction, bioinformatics and cryoET to discover non-canonical bacterial protein translocation-folding complex doi.org/10.1101/2025...
Reposted by Tzu-Jing Yang
Since last year, I've been making a RELION GUI that supports viewing, navigating and running jobs in a way completely compatible with the original RELION pipeline. Now it's working nicely for both SPA and tomography! Please try it out - github.com/hanjinliu/hi...
February 22, 2026 at 8:10 PM
Since last year, I've been making a RELION GUI that supports viewing, navigating and running jobs in a way completely compatible with the original RELION pipeline. Now it's working nicely for both SPA and tomography! Please try it out - github.com/hanjinliu/hi...
Reposted by Tzu-Jing Yang
Ribosomes in pairs: A survival strategy inside stressed cells.
When nutrients drop or temperatures shift, cells switch to survival mode. New work by @erin-schuman.bsky.social & colleagues shows that inactive ribosomes pair up via a ribosomal RNA link @andschwarz.bsky.social @muellermara.bsky.social
When nutrients drop or temperatures shift, cells switch to survival mode. New work by @erin-schuman.bsky.social & colleagues shows that inactive ribosomes pair up via a ribosomal RNA link @andschwarz.bsky.social @muellermara.bsky.social
February 19, 2026 at 7:17 PM
Ribosomes in pairs: A survival strategy inside stressed cells.
When nutrients drop or temperatures shift, cells switch to survival mode. New work by @erin-schuman.bsky.social & colleagues shows that inactive ribosomes pair up via a ribosomal RNA link @andschwarz.bsky.social @muellermara.bsky.social
When nutrients drop or temperatures shift, cells switch to survival mode. New work by @erin-schuman.bsky.social & colleagues shows that inactive ribosomes pair up via a ribosomal RNA link @andschwarz.bsky.social @muellermara.bsky.social
Reposted by Tzu-Jing Yang
Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience
February 12, 2026 at 1:21 PM
Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience
Reposted by Tzu-Jing Yang
New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Nature Protocols, Published online: 30 January 2026; doi:10.1038/s41596-025-01284-zA protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary Drosophila melanogaster neurons.
dlvr.it
January 30, 2026 at 11:25 AM
New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Reposted by Tzu-Jing Yang
FoldMason is out now in @science.org. It generates accurate multiple structure alignments for thousands of protein structures in seconds. Great work by Cameron L. M. Gilchrist and @milot.bsky.social.
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
Multiple protein structure alignment at scale with FoldMason
Protein structure is conserved beyond sequence, making multiple structural alignment (MSTA) essential for analyzing distantly related proteins. Computational prediction methods have vastly extended ou...
www.science.org
January 30, 2026 at 6:11 AM
FoldMason is out now in @science.org. It generates accurate multiple structure alignments for thousands of protein structures in seconds. Great work by Cameron L. M. Gilchrist and @milot.bsky.social.
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
📄 www.science.org/doi/10.1126/...
🌐 search.foldseek.com/foldmason
💾 github.com/steineggerla...
Reposted by Tzu-Jing Yang
I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...
#virology #teamtomo
www.nature.com/articles/s41...
Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation - Nature Communications
In this study, Dahmane et al use a method called cryo-electron tomography to uncover new details of how tick-borne flaviviruses transform cells into virus factories.
www.nature.com
January 23, 2026 at 4:47 PM
I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...
#virology #teamtomo
www.nature.com/articles/s41...
Reposted by Tzu-Jing Yang
LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.
🔗 doi.org/10.64898/202...
#CryoET
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.
🔗 doi.org/10.64898/202...
#CryoET
January 20, 2026 at 10:01 AM
LLOMe has long been used to study lysosomal damage, yet how it works has remained a mystery.
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.
🔗 doi.org/10.64898/202...
#CryoET
Using cryo-electron tomography, we show it forms amyloid structures inside lysosomes that mechanically rupture membranes – revealing a new paradigm for lysosomal failure.
🔗 doi.org/10.64898/202...
#CryoET
Reposted by Tzu-Jing Yang
We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
January 13, 2026 at 2:39 PM
We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
Reposted by Tzu-Jing Yang
Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
January 12, 2026 at 7:57 PM
Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7)
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Tzu-Jing Yang
The last chapter from my postdoc days is finally online! We solve structures of the ER membrane O-mannosyltransferase Pmt4 and uncover a functionally important cytosolic binding site for the Dol-P-Man substrate lipid - allosteric regulation or a long sought after Dol-P-Man flippase?? 🔄 rdcu.be/eUJfb
December 16, 2025 at 10:12 AM
The last chapter from my postdoc days is finally online! We solve structures of the ER membrane O-mannosyltransferase Pmt4 and uncover a functionally important cytosolic binding site for the Dol-P-Man substrate lipid - allosteric regulation or a long sought after Dol-P-Man flippase?? 🔄 rdcu.be/eUJfb
Reposted by Tzu-Jing Yang
It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
December 16, 2025 at 8:17 AM
It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
Reposted by Tzu-Jing Yang
ExoSloNano: nanogold probe-based labeling of proteins in live cells for cryo-ET and CLEM. @lindseynyoung.bsky.social @thevillalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms - Nature Methods
The ExoSloNano system facilitates nanogold probe-based labeling of specific proteins of a wide range of sizes in live cells for cryo-electron tomography and correlative light and electron microscopy s...
www.nature.com
December 11, 2025 at 4:16 PM
ExoSloNano: nanogold probe-based labeling of proteins in live cells for cryo-ET and CLEM. @lindseynyoung.bsky.social @thevillalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tzu-Jing Yang
@attychang.bsky.social, @nanigrotjahn.bsky.social et al. show that cytoplasmic #ribosomes on #mitochondria alter the local membrane environment for protein import. rupress.org/jcb/article/...
📕 From The Year In Cell Biology: rupress.org/jcb/collecti...
#CellBio2025
📕 From The Year In Cell Biology: rupress.org/jcb/collecti...
#CellBio2025
December 5, 2025 at 4:15 PM
@attychang.bsky.social, @nanigrotjahn.bsky.social et al. show that cytoplasmic #ribosomes on #mitochondria alter the local membrane environment for protein import. rupress.org/jcb/article/...
📕 From The Year In Cell Biology: rupress.org/jcb/collecti...
#CellBio2025
📕 From The Year In Cell Biology: rupress.org/jcb/collecti...
#CellBio2025
Reposted by Tzu-Jing Yang
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
November 6, 2025 at 7:38 AM
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...