#ChIPSeq
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epistrucstab.bsky.social
🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Image credit: @gloglita.bsky.social‬ @lifescienceeditors.bsky.social‬ captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes
July 28, 2025 at 9:10 AM
haoyin.bsky.social
TBX4 in human Lung Pericyte & Fibroblast (matrix, motility, guidance molecules, cell cycle)

scRNAseq developing mouse lung
Bulk RNAseq + ChIPseq cultured human lung pericyte & fibroblast -/+ siTBX4

#PulmCirc 2025
onlinelibrary.wiley.com/doi/10.1002/...
March 4, 2025 at 7:48 PM
haoyin.bsky.social
"an accurate pan-tissue, pan-histone-mark age predictor"😎

1814 ChIPseq samples
H3K4me3
H3K27ac
H3K9ac
H3K9me3
H3K27me3
H3K36me3
H3K4me1

82 tissues (incld large arteries)

"only a small fraction of the 62,241 features is sufficient to predict age"

#SciAdv 2024
www.science.org/doi/10.1126/...
January 2, 2025 at 1:00 PM
pavelkrav.bsky.social
(14/14) Feedback very welcome!
cc @pensarata.bsky.social
@halfacrocodile.bsky.social
@vorontsovie.bsky.social
@sevamakeev.bsky.social

#bioinformatics #compbio #genomics #ChIPseq #TFBS #MachineLearning #RandomForest
May 24, 2026 at 11:23 AM
xgrau.bsky.social
New preprint from the @arnausebe.bsky.social lab! 💐

Here @crisnava.bsky.social, @seanamontgomery.bsky.social & collaborators develop a novel ChIPseq protocol, and demonstrate its huge potential to study the evolution of chromatin function and regulation across the eukaryotic tree of life.
Figure 1 from the paper, with two panels. Panel a shows a schematic cladogram of the eukaryotic tree of life with an adjacent table showing the presence/absence of various histone post-translational modifications in various lineages. Panel b is a summary of the multiplexing strategy for ChIP-seq experiments developed in the paper.
March 19, 2025 at 10:31 AM
robertife.bsky.social
Please RT 📧
Registration open for ➡️"Summer School on Chromatin Biology" Our 2 weeks hands on expedition 🧪👩‍💻August 2026. 3. edition. Learn CUT@Tag, CUT@Run, ChIPseq, ATACseq AND to analyse your own data at @helmholtzmunich.bsky.social Daily letures by experts in the field !

▶️ shorturl.at/jrA8i
December 9, 2025 at 11:59 AM
chansenwheat.bsky.social
JOB ALERT!

What is the cost of making color in butterfly wings?

Postdoc opening studying life-history tradeoffs using function genomics (CRISPR, ATACseq, CHIPseq, ssRNAseq) at Stockholm University.

t.co/ToErH8ITM5

🌍🧪 #AcademicSky #HigherEd #WomeninSTEM #EcoECRcareer
February 8, 2024 at 8:46 AM
marcrr.bsky.social
Update of our preprint on detecting selection on regulatory sequences! We notably added an analysis for human, where the likelihood test per ChIPseq peak lacks power.
www.biorxiv.org/content/10.1...
March 6, 2026 at 2:15 PM
babraham-bioinf.bsky.social
Check out our #TrainingTuesday #Bioinformatics and #statistics training opportunities
www.bioinformatics.babraham.ac.uk/training.html
We have #ChIPSeq and #ATACSeq data analysis training coming up! #ComputationalBiology #ResearchScientce #ECR #PhD
Training course dates and topics as per link
February 11, 2025 at 12:39 PM
romangp.bsky.social
New preprint from the Lab at @cabimer.bsky.social. We present PLAMseq, using TurboID to do like ChIPseq and proximity proteomics in the same protocol with extra powers like mapping protein interactions in the genome.

www.biorxiv.org/content/10.1...
PLAMseq enables the proteo-genomic characterization of chromatin-associated proteins and protein interactions in a single experimental workflow.
Chromatin Immunoprecipitation (ChIP) and Co-Immunoprecipitation (CoIP) assays are the most common approaches to characterize the genomic localization and protein interactors, respectively, for a prote...
www.biorxiv.org
April 28, 2025 at 7:55 AM
haoyin.bsky.social
MetaLoci
👉Spatial autocorrelation analysis for Hi-C + H3K27ac ChIPseq
👉No assumption on pre-defined genomic features - A/B compartment, TAD, chromatin loop▶️
A gonad-specific 3D regulatory element (250 kb downstream🤠) of Fgf9 gene in 🐭Sex determination

#NatSMB 2026
www.nature.com/articles/s41...
February 26, 2026 at 12:45 PM
haoyin.bsky.social
🤵Artery Super-enhancer (H3K27ac ChIPseq) analysis (127 TFs)▶️
PRDM16 as a positive regulator of Injury-induced (not developmental) Proliferation of Vascular Smooth Muscle Cell😎

Any role in SMC-to-brown adipocyte conversion?😁

@jiliang-zhou.bsky.social #JMCC 2026
www.sciencedirect.com/science/arti...
March 18, 2026 at 7:26 PM
jcarroll42.bsky.social
Here's an update on our story of doing CHIPSeq for the Huntingtin protein. Really interesting stuff, as it suggests HTT is binding to chromatin in patterns that are coherent with gene expression changes in HD, suggesting HTT might play a direct role in these changes.
jeffcantle.bsky.social jeffcantle.bsky.social @jeffcantle.bsky.social · Jan 7
Promoting this freshly revised HTT-chromatin interaction preprint that I've been working on with @jcarroll42.bsky.social as my first skeet. www.biorxiv.org/content/10.1...
January 7, 2025 at 7:49 PM
biotechniques.bsky.social
Scientists have created PLAMseq – a new method to enable proteogenomic characterization of genome proteins and their interactions in a single workflow >>> www.biotechniques.com/proteomics/i... 🧪
Introducing PLAMseq: new sequencing method transforms proteogenomic characterization
A new alternative to CHIPseq performs proteomics and genomics in the same workflow.
www.biotechniques.com
December 16, 2025 at 3:41 PM
epigenometech.bsky.social
Our expansion of single-cell epigenetics assays continues!

The Epigenome Technologies scCUT&Tag assay is now compatible with the BD Rhapsody platform

#Epigenetics #BDrhapsody #CUTandTag #Chromatin #Multiomics #Singlecell #CUTandRUN #ChIPseq #ATACseq

Learn more here: u.epigenome.us/NYjSGQao
August 13, 2026 at 8:01 PM
activemotifusa.bsky.social
🎉 The wait is over — say hello to the TIP-ChIP™ Assay Kit! [https://bit.ly/47Fpvjf] A high-throughput, low-input alternative to traditional ChIP that streamlines your workflow and boosts your data quality.
_____
#TIPChIP #Epigenetics #ChIPSeq #NGS #MolecularBiology #LabTools #Genomics #ActiveMotif
November 13, 2025 at 10:19 PM
epigenometech.bsky.social
Learn more about single-cell epigenetic profiling by downloading our guide to single-cell CUT&Tag and single-cell Paired-Tag, which provides comparisons to other methods and bioinformatic insight

bit.ly/4r7CGRD

#Epigenetics #Multiomics #CUTandTag #10XGgenomics #ChIPSeq #SingleCell #PairedTag
March 3, 2026 at 4:49 PM
haoyin.bsky.social
Map of #QuantitativeTraitLoci for TCF21 binding (ChIPseq), chromatin accessibility (ATACseq), & chromosomal looping (Hi-C) in 👤coronary artery SMC

CRISPRi (dCas9-KRAB) to assess allele-specific effects

Thomas Quertermous lab #GenomBiol 2020
genomebiology.biomedcentral.com/articles/10....
January 2, 2026 at 8:22 PM
epigenometech.bsky.social
Learn more about single-cell epigenetic profiling by downloading our guide to single-cell CUT&Tag and single-cell Paired-Tag, which provides comparisons to other methods and bioinformatic insight!

bit.ly/4r7CGRD

#Epigenetics #Multiomics #CUTandTag #10XGgenomics #ChIPSeq #SingleCell #PairedTag
November 21, 2025 at 3:03 PM
marcrr.ecoevo.social.ap.brid.gy
RE: https://ecoevo.social/@marcrr/115656029348896562

Update of our preprint on detecting selection on regulatory sequences! We notably added an analysis for human, where the likelihood test per ChIPseq peak lacks power.
There is a similar problem with branch-site dN/dS tests, and a solution has […]
March 6, 2026 at 2:05 PM
juliebiteenlab.bsky.social
Lauren, Lindsay, Xiaofeng et al. coupled live-cell super-resolution microscopy with ChIPseq and Hi-C in starved E. coli to learn that the abundant protein Dps compacts DNA while only minimally affecting other nucleoid properties. XindanWang Lab & @meyerroc.bsky.social
www.biorxiv.org/content/10.1...
Dps binds and protects DNA in starved Escherichia coli with minimal effect on chromosome accessibility, dynamics and organisation
Dps is the most abundant nucleoid-associated protein in starved Escherichia coli with ∼180, 000 copies per cell. Dps binds DNA and oxidises iron, facilitating survival in harsh environments. Dps-DNA c...
www.biorxiv.org
September 15, 2025 at 12:55 PM
scotomorph.bsky.social
G4s mapped in T. brucei using ChIPseq - finally!
biorxivpreprint.bsky.social bioRxivpreprint @biorxivpreprint.bsky.social · Jul 23
Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions https://www.biorxiv.org/content/10.1101/2025.07.22.666098v1
July 23, 2025 at 9:07 AM
epigenometech.bsky.social
Learn about single-cell epigenetic profiling by downloading our guide to single-cell CUT&Tag/Paired-Tag, which provides comparisons to other methods and bioinformatic insight

Learn more: bit.ly/4r7CGRD

#Epigenetics #Multiomics #CUTandTag #10XGgenomics #ChIPSeq #SingleCell #PairedTag
May 15, 2026 at 2:59 PM
epigenometech.bsky.social
Learn more about single-cell epigenetic profiling by downloading our guide to single-cell CUT&Tag and Paired-Tag, which provides comparisons to other methods and bioinformatic insight

#Epigenetics #Multiomics #CUTandTag #10XGgenomics #ChIPSeq #SingleCell #PairedTag

u.epigenome.us/mHSAqLpl
July 7, 2026 at 2:53 PM
pradeepmadapura.bsky.social
Now that I have read this paper, my lab has new findings based on CUT&Tag data that challenge the traditional view of euchromatic and repressive factors. I have started doubting all previous ChIPseq-based conclusions for many targets we are interested in. Thanks for publishing the preprint
February 6, 2025 at 5:55 PM