Systematic benchmarking of basecalling models for RNA modification detection with highly-multiplexed nanopore sequencing. #DirectRNAsequencing #DRS #RNAmodifications #Nanopore #Genomics #Bioinformatics #ModelBenchmarking #Basecalling @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...

🧬(1/2) Capturing RNA structure at the single-molecule level🔬NanoRAPID is a deep learning framework that directly analyzes raw nanopore DRS signals to accurately identify RNA structural probing sites.
#Nanopore #DirectRNASequencing #RNAStructure #DeepLearning

(2/2)🧵🔎 Explore, search, and visualize Ψ modifications with built-in tools including PsiVar and PsiFinder.
📖 Read the study
@GPBees
academic.oup.com/gpb/advance-.... #Pseudouridine #RNA #RNAmodification #Bioinformatics #Database #DirectRNASequencing

Enhanced detection of RNA modifications in Escherichia coli utilizing direct RNA sequencing. #RNAmodifications #directRNAsequencing #Nanopore #Sequencing @nanoporetech.com @cp-cellrepmethods.bsky.social 🧬 🖥️
www.cell.com/cell-reports...

Most RNA structure studies show you a crowd snapshot.
sm‑PORE‑cupine lets you watch individuals.

#RNAstructure #Nanopore #DirectRNASequencing #SingleMolecule #RNAseq #Bioinformatics #Genomics #Transcriptomics #ComputationalBiology #NatureMethods #Research #Biology #LifeSciences #Trinobia

This paper by Denise Ohnezeit and the group of Angus Wilson ask a harder question than most.

When can we actually trust m6A calls from nanopore direct RNA sequencing?

Not if. Under what conditions.

#DirectRNASequencing #ONT #Dorado #ScientificRigor #Reproducibility #ComputationalBiology