To recapitulate clinical COVID-19 in human patients, we developed a clinically relevant COVID-19 mouse model in which the original strain of SARS-CoV-2 virus was used to infect immunocompetent heterozygous K18-hACE C57BL/6 J mice.39 All these experiments were performed in a P3 laboratory under the strict regulations of the Karolinska Institutet and the ethical regulations of the Stockholm Ethical Committee. Each of the 10-week-old male heterozygous K18-hACE C57BL/6 J mice received 100 PFU of SARS-CoV-2 virus through intranasal delivery using our previously published protocols.39 On day 7 postinfection, the mice were sacrificed, and the relevant tissues and organs were collected for their respective analyses. Gross examination of lung tissues showed increased shadier color in the SARS-CoV-2-infected lungs relative to the noninfected controls (Fig. 1a). The total tissue weight of the SARS-CoV-2-infected lungs was also significantly increased (Fig. 1a). Hematoxylin & eosin (H&E) staining of lung tissues demonstrated marked increases in cellular components in SARS-CoV-2-infected lungs, resulting in decreased diameters of the pulmonary alveolus (Fig. 1b). Extravasation of plasma fluid together with other cellular components was frequently found in the alveolar space (Fig. 1b). One of the distinguished features was the markedly increased density of CD31+ microvessels in SARS-CoV-2-infected lung tissues (Fig...

Background. Mitophagy is the cellular removal of unwanted mitochondria via the lysosome. Given the importance of this process to energy demanding tissues, mitophagy defects have been linked to various metabolic and neurodegenerative diseases. Mitophagy assessment tools are important f...

Spinal cord injury (SCI) often results in severe hypoxia and excessive activation of neuroinflammation, which aggravates neuropathology and neurological dysfunction. D-dopachrome tautomerase (D-DT), the homolog of macrophage migration inhibitory factor (MIF), is a key proinflammatory mediator implicated in inflammatory diseases of multiple tissues. However, its relation with hypoxia and the potential impact on neuroinflammation following SCI remain elusive. Herein, the dynamic expression of D...

Read the full paper: Hypoxia Promotes Pancreatic Cancer Malignancy via HIF-1α-induced Doublecortin-like Kinase 1 Upregulation

Journal of the peripheral nervous system : JPNS · An S, Shi J, Huang J, Li Z, Cheng J, Feng M.

Professor Rikke Olsen Aarhus University, Denmark/EMERG Hypoxia-Induced Mitochondrial Stress-Signaling: A Randomised Controlled Trial for ME

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