Please see our latest paper on the role of EXO1 in meiosis: "EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA". Congratulations to both first authors, Megha Roy and Aurore Sanchez and thanks to all our collaborators!
May 5, 2025 at 7:22 AM
Please see our latest paper on the role of EXO1 in meiosis: "EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA". Congratulations to both first authors, Megha Roy and Aurore Sanchez and thanks to all our collaborators!
March 5, 2026 at 1:36 AM
New study from the lab @hannahtrost.bsky.social Functions of PMS2 and MLH1 important for regulation of divergent repea... www.sciencedirect.com/science/arti...
Functions of PMS2 and MLH1 important for regulation of divergent repeat-mediated deletions
Repeat-mediated deletions (RMDs) are a type of deletion rearrangement that utilizes two repetitive elements to bridge a DNA double-strand break (DSB) …
www.sciencedirect.com
November 29, 2024 at 2:17 PM
New study from the lab @hannahtrost.bsky.social Functions of PMS2 and MLH1 important for regulation of divergent repea... www.sciencedirect.com/science/arti...
Good science news to greet the holidays. Our paper is now out @natcomms.nature.com: www.nature.com/articles/s41.... Outstanding work by two of my postdocs, Aloran and Tyler, and collabs with Nic Fusco, Carol Manhart @templechemistry.bsky.social & Matthew Bainbridge @radychildrensoc.bsky.social 🧵🧪/1
Aberrant cytoplasmic localization of MLH1 characterizes a cell population that seeds breast cancer recurrence - Nature Communications
Mismatch repair pathway is frequently dysregulated across cancer types, commonly represented by loss of MLH1 or MSH2 gene expression. Here the authors model MLH1 missense mutations from patients to st...
www.nature.com
December 15, 2025 at 6:14 PM
Good science news to greet the holidays. Our paper is now out @natcomms.nature.com: www.nature.com/articles/s41.... Outstanding work by two of my postdocs, Aloran and Tyler, and collabs with Nic Fusco, Carol Manhart @templechemistry.bsky.social & Matthew Bainbridge @radychildrensoc.bsky.social 🧵🧪/1
We are delighted to share our latest work, characterising the role of BRCA2 and its binding partner PALB2 at centromeres, just out in the latest issue of Cell Reports. ⬇️
www.cell.com/cell-reports...
www.cell.com/cell-reports...
The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability
Graham et al. demonstrate that BRCA2 and PALB2 protect centromere integrity in non-cancerous
cells through distinct mechanisms, partly counteracting the mismatch repair factor
MLH1. In cancer cells, B...
www.cell.com
February 25, 2025 at 5:56 PM
We are delighted to share our latest work, characterising the role of BRCA2 and its binding partner PALB2 at centromeres, just out in the latest issue of Cell Reports. ⬇️
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Targeting SIRT2 promotes MLH1 acetylation and degradation, increases CD8+ T cell infiltration and activation, and generates antitumor immune memory
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
July 17, 2025 at 2:27 PM
Targeting SIRT2 promotes MLH1 acetylation and degradation, increases CD8+ T cell infiltration and activation, and generates antitumor immune memory
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
#TissueTuesday
Proteintech’s MLH1 Monoclonal Antibody is derived from a clone optimized for high performance in IHC. It has been validated in multiple cancer tissues with whole slide images provided for the most in-depth look at IHC data.
Learn more: https://bit.ly/4jgp0Cg
Proteintech’s MLH1 Monoclonal Antibody is derived from a clone optimized for high performance in IHC. It has been validated in multiple cancer tissues with whole slide images provided for the most in-depth look at IHC data.
Learn more: https://bit.ly/4jgp0Cg
MLH1 antibody (60822-2-Ig) | Proteintech
Proteintech's Mouse Monoclonal MLH1 antibody is validated in IHC, ELISA and shows reactivity with human samples.
bit.ly
September 22, 2026 at 3:48 PM
#TissueTuesday
Proteintech’s MLH1 Monoclonal Antibody is derived from a clone optimized for high performance in IHC. It has been validated in multiple cancer tissues with whole slide images provided for the most in-depth look at IHC data.
Learn more: https://bit.ly/4jgp0Cg
Proteintech’s MLH1 Monoclonal Antibody is derived from a clone optimized for high performance in IHC. It has been validated in multiple cancer tissues with whole slide images provided for the most in-depth look at IHC data.
Learn more: https://bit.ly/4jgp0Cg
🧪 Super excited about our new paper!
We evaluated ChatGPT-4 for genetic info on BRCA1, HFE, and MLH1 which revealed improved accuracy over GPT-3.5 (P < .0001). Errors still persist and ethical implications demand careful tech integration in healthcare. 🩺🖥️🧬 🤖 #LLM
We evaluated ChatGPT-4 for genetic info on BRCA1, HFE, and MLH1 which revealed improved accuracy over GPT-3.5 (P < .0001). Errors still persist and ethical implications demand careful tech integration in healthcare. 🩺🖥️🧬 🤖 #LLM
A comparative evaluation of ChatGPT 3.5 and ChatGPT 4 in responses to selected genetics questions
AbstractObjectives. To evaluate the efficacy of ChatGPT 4 (GPT-4) in delivering genetic information about BRCA1, HFE, and MLH1, building on previous findin
academic.oup.com
June 14, 2024 at 8:20 PM
🧪 Super excited about our new paper!
We evaluated ChatGPT-4 for genetic info on BRCA1, HFE, and MLH1 which revealed improved accuracy over GPT-3.5 (P < .0001). Errors still persist and ethical implications demand careful tech integration in healthcare. 🩺🖥️🧬 🤖 #LLM
We evaluated ChatGPT-4 for genetic info on BRCA1, HFE, and MLH1 which revealed improved accuracy over GPT-3.5 (P < .0001). Errors still persist and ethical implications demand careful tech integration in healthcare. 🩺🖥️🧬 🤖 #LLM
OK, so MLH1 is in the cytoplasm. What does it do there? Expressing patient tumor MLH1 mutations in ER+ breast cancer cells induces endocrine resistance. The cool part is that the mutations are almost always subclonal, but resist standard treatments to become the majority population over time /4
December 15, 2025 at 6:14 PM
OK, so MLH1 is in the cytoplasm. What does it do there? Expressing patient tumor MLH1 mutations in ER+ breast cancer cells induces endocrine resistance. The cool part is that the mutations are almost always subclonal, but resist standard treatments to become the majority population over time /4
#LegacyContent: MLH1 Promotor Hypermethylation in Colorectal and Endometrial Carcinomas from Patients with #LynchSyndrome.
#OpenAccess text: www.jmdjournal.org/article/S152...
#OpenAccess text: www.jmdjournal.org/article/S152...
MLH1 Promotor Hypermethylation in Colorectal and Endometrial Carcinomas from Patients with Lynch Syndrome
Screening for Lynch syndrome (LS) in colorectal cancer (CRC) and endometrial cancer
patients generally involves immunohistochemical staining of the mismatch repair (MMR)
proteins. In case of MLH1 prot...
www.jmdjournal.org
February 27, 2025 at 6:48 PM
#LegacyContent: MLH1 Promotor Hypermethylation in Colorectal and Endometrial Carcinomas from Patients with #LynchSyndrome.
#OpenAccess text: www.jmdjournal.org/article/S152...
#OpenAccess text: www.jmdjournal.org/article/S152...
A novel MLH1 small binder (MLH1-SB) enhances prime editing efficiency by inhibiting mismatch repair, outperforming dominant-negative MLH1 in precision. PMID:40769155, Cell 2025, @Cell https://www.cell.com/cell/fulltext/S0092-8674(25)00799-8 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
https://www.cell.com/cell/fulltext/S0092-8674(25)00799-8
No description available
www.cell.com
August 30, 2025 at 7:00 PM
🧬 Crossover maps of mlh1 mutants reveal weakened interference, increased aneuploidy, and the involvement of helicases in resolving recombination intermediates in the absence of MutLγ. Tight regulation of MLH1/MLH3 levels is crucial for plant fertility!
March 19, 2025 at 2:18 PM
🧬 Crossover maps of mlh1 mutants reveal weakened interference, increased aneuploidy, and the involvement of helicases in resolving recombination intermediates in the absence of MutLγ. Tight regulation of MLH1/MLH3 levels is crucial for plant fertility!
Is Cancer Hereditary? Myths and Facts about Cancer Genetics
oncodaily.com/opinion/270673
#OncoDaily #Oncology #Cancer #Health #Medicine #MedEd #MedOnc #MedNews #BRCA #CancerScreening #CancerFreeFamilies #CFFs #TP53 #MLH1 #FamilialAdenomatousPolyposis #FAP
oncodaily.com/opinion/270673
#OncoDaily #Oncology #Cancer #Health #Medicine #MedEd #MedOnc #MedNews #BRCA #CancerScreening #CancerFreeFamilies #CFFs #TP53 #MLH1 #FamilialAdenomatousPolyposis #FAP
Is Cancer Hereditary? Myths and Facts about Cancer Genetics - OncoDaily
Is cancer hereditary? Learn how inherited mutations impact cancer risk, who should consider genetic testing, and how it informs prevention.
oncodaily.com
April 16, 2025 at 9:42 PM
Is Cancer Hereditary? Myths and Facts about Cancer Genetics
oncodaily.com/opinion/270673
#OncoDaily #Oncology #Cancer #Health #Medicine #MedEd #MedOnc #MedNews #BRCA #CancerScreening #CancerFreeFamilies #CFFs #TP53 #MLH1 #FamilialAdenomatousPolyposis #FAP
oncodaily.com/opinion/270673
#OncoDaily #Oncology #Cancer #Health #Medicine #MedEd #MedOnc #MedNews #BRCA #CancerScreening #CancerFreeFamilies #CFFs #TP53 #MLH1 #FamilialAdenomatousPolyposis #FAP
Other mentionables - Neurofibromatosis Type 1 (medulloblastoma and stomach cancers), Lynch Syndrome (MLH1/2 mutations: Colon, uterine and gastric cancers), Fanconi’s anemia, Ataxia Telangiectasia, Nijmegen breakage syndrome, Sekel Syndrome… list goes on and on
September 2, 2025 at 5:27 PM
Other mentionables - Neurofibromatosis Type 1 (medulloblastoma and stomach cancers), Lynch Syndrome (MLH1/2 mutations: Colon, uterine and gastric cancers), Fanconi’s anemia, Ataxia Telangiectasia, Nijmegen breakage syndrome, Sekel Syndrome… list goes on and on
Check out the preprint of our latest work: www.biorxiv.org/content/10.1...
The mismatch repair factor Mlh1-Pms1 uses ATP to compact and remodel DNA
In eukaryotes, mismatch repair begins with MutS homolog (MSH) complexes, which scan newly replicated DNA for mismatches. Upon mismatch detection, MSH complexes recruit the PCNA- stimulated endonucleas...
www.biorxiv.org
January 17, 2025 at 1:47 PM
Check out the preprint of our latest work: www.biorxiv.org/content/10.1...
Fascinating study (and thread) about the role of cytoplasmic MLH1 in residual tumor cells that go on to seed ER+ breast cancer recurrences
Good science news to greet the holidays. Our paper is now out @natcomms.nature.com: www.nature.com/articles/s41.... Outstanding work by two of my postdocs, Aloran and Tyler, and collabs with Nic Fusco, Carol Manhart @templechemistry.bsky.social & Matthew Bainbridge @radychildrensoc.bsky.social 🧵🧪/1
Aberrant cytoplasmic localization of MLH1 characterizes a cell population that seeds breast cancer recurrence - Nature Communications
Mismatch repair pathway is frequently dysregulated across cancer types, commonly represented by loss of MLH1 or MSH2 gene expression. Here the authors model MLH1 missense mutations from patients to st...
www.nature.com
December 15, 2025 at 8:06 PM
Fascinating study (and thread) about the role of cytoplasmic MLH1 in residual tumor cells that go on to seed ER+ breast cancer recurrences
Our EpiMelt MLH1 Human Methylation Detection Assay was used in a study to determine if MS-HRM is a simple & sensitive real-time PCR method to detect MLH1 epimutation. #epigenetics #DNAMethylation
See it: bocascientific.com/products/epi...
clinicalepigeneticsjournal.biomedcentral.com/articles/10....
See it: bocascientific.com/products/epi...
clinicalepigeneticsjournal.biomedcentral.com/articles/10....
EpiMelt MLH1 Human Methylation Detection Assay
EpiMelt MLH1 Human Methylation Detection Assay EpiMelt Assays Cat#: MD-MLH1-200
bocascientific.com
July 21, 2025 at 3:11 PM
Our EpiMelt MLH1 Human Methylation Detection Assay was used in a study to determine if MS-HRM is a simple & sensitive real-time PCR method to detect MLH1 epimutation. #epigenetics #DNAMethylation
See it: bocascientific.com/products/epi...
clinicalepigeneticsjournal.biomedcentral.com/articles/10....
See it: bocascientific.com/products/epi...
clinicalepigeneticsjournal.biomedcentral.com/articles/10....
The National Comprehensive Cancer Network (NCCN) guidelines recommend genetic testing for ovarian cancer susceptibility genes (ATM, BRCA1, BRCA2, BRIP1, MLH1, MSH2, MSH6, EPCAM, PALB2, RAD51C, and RAD51D) in individuals
July 21, 2025 at 10:28 PM
The National Comprehensive Cancer Network (NCCN) guidelines recommend genetic testing for ovarian cancer susceptibility genes (ATM, BRCA1, BRCA2, BRIP1, MLH1, MSH2, MSH6, EPCAM, PALB2, RAD51C, and RAD51D) in individuals
Image of the Week!
Submitted by: Idd Shabani, M.D.
Directory Profile: buff.ly/uFBf0hx
Subspecialty: Stains & CD markers (IHC)
Diagnosis: Histological image showing a mismatch repair protein (MLH1) proficient colorectal cancer
#Pathology #PathologyOutlines #PathSky
Submitted by: Idd Shabani, M.D.
Directory Profile: buff.ly/uFBf0hx
Subspecialty: Stains & CD markers (IHC)
Diagnosis: Histological image showing a mismatch repair protein (MLH1) proficient colorectal cancer
#Pathology #PathologyOutlines #PathSky
March 26, 2025 at 6:04 PM
Image of the Week!
Submitted by: Idd Shabani, M.D.
Directory Profile: buff.ly/uFBf0hx
Subspecialty: Stains & CD markers (IHC)
Diagnosis: Histological image showing a mismatch repair protein (MLH1) proficient colorectal cancer
#Pathology #PathologyOutlines #PathSky
Submitted by: Idd Shabani, M.D.
Directory Profile: buff.ly/uFBf0hx
Subspecialty: Stains & CD markers (IHC)
Diagnosis: Histological image showing a mismatch repair protein (MLH1) proficient colorectal cancer
#Pathology #PathologyOutlines #PathSky
Metachronous colorectal cancer risks after extended or segmental resection in MLH1, MSH2, and MSH6 Lynch syndrome: multicentre study from the Prospective Lynch Syndrome Database
➡️ doi.org/10.1093/bjs/...
➡️ doi.org/10.1093/bjs/...
Metachronous colorectal cancer risks after extended or segmental resection in MLH1, MSH2, and MSH6 Lynch syndrome: multicentre study from the Prospective Lynch Syndrome Database
This first prospective observational study evaluates the impact of extended versus segmental colorectal surgery on the risk of metachronous colorectal canc
doi.org
April 22, 2025 at 11:16 AM
Metachronous colorectal cancer risks after extended or segmental resection in MLH1, MSH2, and MSH6 Lynch syndrome: multicentre study from the Prospective Lynch Syndrome Database
➡️ doi.org/10.1093/bjs/...
➡️ doi.org/10.1093/bjs/...
So happy to have contributed to this work! rdcu.be/eyv8r
MLH1 c.27G>A (p.Arg9=) is a synonymous likely/pathogenic variant underlying variably mosaic constitutional MLH1 methylation in Lynch syndrome
rdcu.be
July 30, 2025 at 12:36 PM
So happy to have contributed to this work! rdcu.be/eyv8r
What are germline mutations and what role do they play in hereditary cancer?
The infographic can be downloaded at: genetionary.org/resources/
#HereditaryCancerWeek #GermlineMutations #Cancer #GeneticTesting #LynchSyndrome #BRCA2 #BRCA1 #CHEK2 #ATM #PALB2 #PTEN #MLH1 #MSH2 #MSH6 #TP53 #DNA
The infographic can be downloaded at: genetionary.org/resources/
#HereditaryCancerWeek #GermlineMutations #Cancer #GeneticTesting #LynchSyndrome #BRCA2 #BRCA1 #CHEK2 #ATM #PALB2 #PTEN #MLH1 #MSH2 #MSH6 #TP53 #DNA
September 29, 2025 at 2:37 PM
What are germline mutations and what role do they play in hereditary cancer?
The infographic can be downloaded at: genetionary.org/resources/
#HereditaryCancerWeek #GermlineMutations #Cancer #GeneticTesting #LynchSyndrome #BRCA2 #BRCA1 #CHEK2 #ATM #PALB2 #PTEN #MLH1 #MSH2 #MSH6 #TP53 #DNA
The infographic can be downloaded at: genetionary.org/resources/
#HereditaryCancerWeek #GermlineMutations #Cancer #GeneticTesting #LynchSyndrome #BRCA2 #BRCA1 #CHEK2 #ATM #PALB2 #PTEN #MLH1 #MSH2 #MSH6 #TP53 #DNA
Treatment resistance is caused by a lack of cell cycle control in cells where MLH1 is restricted to the cytoplasm -- it has to be able to get to the nucleus to induce a cell cycle arrest in response to standard treatments for ER+ breast cancer patients. Primary consequence is unregulated CDK4 /5
December 15, 2025 at 6:14 PM
Treatment resistance is caused by a lack of cell cycle control in cells where MLH1 is restricted to the cytoplasm -- it has to be able to get to the nucleus to induce a cell cycle arrest in response to standard treatments for ER+ breast cancer patients. Primary consequence is unregulated CDK4 /5
But why is MLH1 in the cytoplasm given it's a DNA repair protein? We found that MLH1 missense mutations found in patient tumors can induce cyto localization by preventing MLH1 binding to PMS2 which is required to expose its NLS. Low levels of PMS2 have the same effect /3
December 15, 2025 at 6:14 PM
But why is MLH1 in the cytoplasm given it's a DNA repair protein? We found that MLH1 missense mutations found in patient tumors can induce cyto localization by preventing MLH1 binding to PMS2 which is required to expose its NLS. Low levels of PMS2 have the same effect /3