#NatureProtocols
We are go! Follow the new #NatureReviews journals and #NatureProtocols starter pack

go.bsky.app/KcxvQLp

🧪 @natureportfolio.bsky.social
December 18, 2024 at 12:25 PM
CellRank protocol now in @NatureProtocols! 🎉

@PhilippWeiler's detailed guide shows how to:
- Integrate velocity, pseudotime & time points
- Infer cell fate probabilities
- Analyze lineage priming at scale

Great complement to our CellRank 2 paper!

📖 www.nature.com/articles/s41...
February 5, 2026 at 7:37 PM
Web-based multi-omics suite by Jianguo Xia lab

ExpressAnalyst➡️Transcriptomics/Proteomics
MetaboAnalyst➡️Metabolomics

OmicsNet, OmicsAnalyst➡️Mutiomics integration
omicsnet.ca
www.omicsanalyst.ca

+miRNet mGWAS-Explorer MicrobiomeAnalyst

#NatureProtocols 2024
www.nature.com/articles/s41...
October 17, 2025 at 12:13 PM
Learn how to predict mono-, multi-mers and conformations with the ColabFold-AlphaFold2 protocol now out in @NatureProtocols. Right in time for the Nobel Prize! It covers workflows for beginners and advanced concepts.
🚀https://rdcu.be/dWViQ
📄https://www.nature.com/articles/s41596-024-01060-5 🧬🧶
October 14, 2024 at 3:29 PM
Linked Inference of Genomic Experimental Relationships (LIGER)

#MultiomicsIntegration

Integrative nonnegative matrix factorization▶️ latent factor/metagenes

github.com/welch-lab/li...

#NatureProtocols 2020
www.nature.com/articles/s41...
July 26, 2026 at 12:53 PM
Super happy to share our latest work! A detailed protocol for #MicroED sample prep available @NatureProtocols! It covers #FIB, grid making, sample handling, #CryoFLM, and more! Hope it helps!

www.nature.com/articles/s41...
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM - Nature Protocols
We present a protocol for preparing microcrystal samples for cryo-EM diffraction imaging, including room temperature solid-state small molecules and soluble and membrane protein crystals.
www.nature.com
December 20, 2024 at 5:19 PM
Amazing😎Hematoxylin/Eosin-Mimetic NonDestructive 3D Histology of FFPE archived tissue

TOPRO3+Eosin➡️
Optical clearing with ethyl cinnamate➡️
Light-sheet microscopy➡️
Converted to HE-like image with FalseColor-Python➡️
Bioimage analysis with Napari

#NatureProtocols 2024
www.nature.com/articles/s41...
March 11, 2024 at 7:28 PM
I am working on a protocol that recommends using containers to install/execute the software. I remembered that we published a review on packaging and containerisation.
Packaging and containerization of computational methods. www.nature.com/articles/s41...

(Bronwen@NatureProtocols)
Packaging and containerization of computational methods - Nature Protocols
Attempts to reproduce the computational steps described in published omics research often fail. This review provides guidelines for the packaging and containerization of software so that readers can u...
www.nature.com
April 28, 2025 at 12:55 PM
Pysodb
protocols-pysodb.readthedocs.io/en/latest/

A Python package to mine/integrate the data in
#SpatialOmicsDatabase SODB+SOView (31 spatial technologies, >3000 datasets)
gene.ai.tencent.com/SpatialOmics/

#NatureProtocols 2023
www.nature.com/articles/s41...
January 12, 2024 at 12:53 PM
A protocol describing computational tools (kallisto, bustools and kb-python) for single-cell and single-nucleus RNA-seq preprocessing www.nature.com/articles/s41... #NatureProtocols
kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq - Nature Protocols
Kallisto, bustools and kb-python are a set of tools for quantifying bulk, single-cell and single-nucleus RNA-seq. Together, this set of free, open-source software tools can produce gene expression qua...
www.nature.com
November 25, 2024 at 4:43 AM
A steadfast advocate of open science, @pleinerlab.bsky.social makes his molecular tools generalizable & widely available, as in this paper in NatureProtocols www.nature.com/articles/s41... fin/n
A nanobody-based strategy for rapid and scalable purification of human protein complexes - Nature Protocols
Structural and functional analysis of human proteins requires their isolation in high yields and purity. Here, protease-cleavable, high-affinity, tag-specific nanobodies are used for the isolation of ...
www.nature.com
October 27, 2025 at 11:33 PM
A protocol describing the preparation of ultrastable polymeric monomicelle building units, the construction of a library of ultrasmall organic–inorganic nanohybrids, the superassembly of monomicelles into hierarchical superstructures. #NatureProtocols

www.nature.com/articles/s41...
Versatile synthesis of uniform mesoporous superparticles from stable monomicelle units - Nature Protocols
This protocol presents a method for generating mesoporous superparticles from monomicelle units, with precise control of the number of the monomicelle units and the derived mesopores for superparticle...
www.nature.com
November 25, 2024 at 4:51 AM
. @NatureProtocols Optimal 13C NMR investigation of IDPs at 1.2 GHz, Marco Schiavina*, Lorenzo Bracaglia, Maria Anna Rodella, Rainer Kümmerle, Robert Konrat, Isabella C. Felli* & Roberta Pierattelli* @cerm_cirmmp @UNI_FIRENZE www.nature.com/articles/s41... #NMRchat #NMR 🧲
Optimal 13C NMR investigation of intrinsically disordered proteins at 1.2 GHz - Nature Protocols
The recently available 28.2 Tesla nuclear magnetic resonance spectrometers (1.2 GHz 1H frequency) can be used to characterize highly flexible proteins and protein regions. This protocol explains how t...
www.nature.com
December 12, 2023 at 3:53 PM
In honor of #WorldAsthmaDaymonth browse #asthma researchfrom @Springernature @nature @BioMedCentral @NewAdisInsight @NanoNatResearch Incl. Dr. Ramachandran talking about laboratory procedures @SProtocols @NatureProtocols @naturemethods

goo.gl/BxBBR4
Goo.gl is sunsetting
goo.gl
January 15, 2025 at 4:18 PM
PITCh: Improved gene targeting using microhomology mediated endjoining and CRISPR
@NatureProtocols
http://goo.gl/EEwMqB
Goo.gl is sunsetting
goo.gl
November 23, 2024 at 10:58 PM
Out now in @NatureProtocols, a tutorial series using #oxView to design D(R)NA/protein nanostructures and relax them for @ox_DNA simulation! Awesome work by @progolab, @aatmilk, @petr_sulc et al

A quick overview of contents:

(OA link at end of...
November 12, 2024 at 5:47 AM
Check out this @NatureProtocols paper online today from some of our @ISTT_TG members: https://www.nature.com/articles/nprot.2017.153
February 19, 2025 at 1:24 PM
Interrogation of the mammalian gut–brain axis using LC–MS/MS-based targeted metabolomics with in vitro bacterial and organoid cultures and in vivo gnotobiotic mouse models @NatureProtocols https://www.nature.com/articles/s41596-022-00767-7
Interrogation of the mammalian gut–brain axis using LC–MS...
Targeted liquid chromatography–tandem mass spectrometry-b...
www.nature.com
November 25, 2024 at 8:29 AM
btw I missed this nice use and shoutout to BOP LUTs in @calebadawson et al's @NatureProtocols detailing intravital microscopy in mouse mammary tissue: https://www.nature.com/articles/s41596-020-00473-2?proof=t%253B
January 2, 2025 at 4:27 PM
Look who's there in the "Reviews" section @Roy_Lab_Thinks @pankajdubey26 https://twitter.com/NatureProtocols/status/993816024368721921
December 29, 2024 at 5:20 PM
Massive @NatureProtocols detailing DNA-PAINT procedures, including new software from the Jungmann lab http://www.nature.com/nprot/journal/v12/n6/full/nprot.2017.024.html
December 28, 2024 at 4:56 PM
Also, plasmids are on @addgene and a protocol will appear in @NatureProtocols soon #GDRSRday https://twitter.com/CH3W13/status/811483497181364224
December 28, 2024 at 4:03 PM