#NewNProt: A stretchable #nanoelectronic mesh which expands in a growing #organoid enables long-term #electricaldata acquisition bit.ly/3FNdCfD
Cyborg organoids integrated with stretchable nanoelectronics can be functionally mapped during development - Nature Protocols
The integration of stretchable mesh nanoelectronics with stem cells enables the creation of cyborg organoid tissue, which can be longitudinally characterized during development to improve organoid mod...
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April 3, 2025 at 7:09 AM
#NewNProt: A stretchable #nanoelectronic mesh which expands in a growing #organoid enables long-term #electricaldata acquisition bit.ly/3FNdCfD
#NewNProt: #MolecularMRI using deep MR fingerprinting provides quantitative #biomarkers for proteins, metabolites & pH bit.ly/4ljmLwT
Quantitative molecular imaging using deep magnetic resonance fingerprinting - Nature Protocols
A comprehensive protocol for designing and performing magnetic resonance fingerprinting from chemical exchange saturation transfer and semi-solid magnetization transfer imaging using artificial intell...
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April 3, 2025 at 12:48 PM
#NewNProt: #MolecularMRI using deep MR fingerprinting provides quantitative #biomarkers for proteins, metabolites & pH bit.ly/4ljmLwT
#NewNProt: Construction of #RNAseq reference materials for standardising the quantification of #omics data across different instruments and methods bit.ly/41G83Z7
Construction of RNA reference materials for improving the quantification of transcriptomic data - Nature Protocols
The Quartet reference materials enable the integration and standardization of multiomics datasets acquired over a variety of instruments, samples and methods and the quantitative comparison of RNA seq...
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March 3, 2025 at 8:45 PM
#NewNProt: Construction of #RNAseq reference materials for standardising the quantification of #omics data across different instruments and methods bit.ly/41G83Z7
#NewNProt for unraveling #cellcellcommunication with #NicheNet by inferring active ligands from #transcriptomics data bit.ly/41FP0xK
Unraveling cell–cell communication with NicheNet by inferring active ligands from transcriptomics data - Nature Protocols
NicheNet is a computational tool to infer the ligand–receptor interactions that potentially regulate gene expression changes in receiver cell populations, by producing a list of prioritized ligands an...
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March 13, 2025 at 8:28 AM
#NewNProt for unraveling #cellcellcommunication with #NicheNet by inferring active ligands from #transcriptomics data bit.ly/41FP0xK
#NewNProt: Protocol Extension describing joint spatially resolved epigenomic and transcriptomic profiling on a tissue section using #microfluidic chips bit.ly/4hLs7xT #spatialtranscriptomics #spatialepigenomics see also associated News & Views
Spatially resolved genome-wide joint profiling of epigenome and transcriptome with spatial-ATAC-RNA-seq and spatial-CUT&Tag-RNA-seq - Nature Protocols
In this Protocol Extension, the authors describe procedures for spatial barcoding in tissue sections using a microfluidic chip followed by sequencing-based joint genome-wide profiling of transcriptome...
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March 27, 2025 at 9:47 AM
#NewNProt: Protocol Extension describing joint spatially resolved epigenomic and transcriptomic profiling on a tissue section using #microfluidic chips bit.ly/4hLs7xT #spatialtranscriptomics #spatialepigenomics see also associated News & Views
#NewNProt for designing, synthesizing and purifying transfection-ready small circular double-stranded #DNA templates for expression of #gRNAs bit.ly/3DiBPtf
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing - Nature Protocols
Oliynyk and Church present a protocol for designing, synthesizing and purifying transfection-ready, small, circular, double-stranded DNA templates for expression of gRNAs for different genome-editing ...
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March 6, 2025 at 8:56 AM
#NewNProt for designing, synthesizing and purifying transfection-ready small circular double-stranded #DNA templates for expression of #gRNAs bit.ly/3DiBPtf
#NewNProt for mRNA #lipidnanoparticle formulation, characterization, and evaluation bit.ly/4kSBj6d #mRNALNP
mRNA lipid nanoparticle formulation, characterization and evaluation - Nature Protocols
Lipid nanoparticles have emerged as leading candidates for encapsulating mRNA drugs and have shown success in in vivo applications. Here, the authors provide a step-by-step guide for mRNA lipid nanopa...
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March 19, 2025 at 11:10 AM
#NewNProt for mRNA #lipidnanoparticle formulation, characterization, and evaluation bit.ly/4kSBj6d #mRNALNP
#NewNProt: live-cell synthesis of #quantumdots overcomes the limited biocompatibility of their conventionally synthesized counterparts bit.ly/3RhxUAi
Live-cell synthesis of biocompatible quantum dots - Nature Protocols
Quantum dots can be synthesized in live yeast, bacterial and mammalian cells by controlling the temporal and spatial pattern of specific metabolic reactions via the timed supply of chemicals.
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March 20, 2025 at 2:17 PM
#NewNProt: live-cell synthesis of #quantumdots overcomes the limited biocompatibility of their conventionally synthesized counterparts bit.ly/3RhxUAi
#NewNProt for Peptide-enabled #ribonucleoprotein delivery for #CRISPR engineering (PERC) for ex vivo #genomeediting of primary human cells bit.ly/3DdsrY0
Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells - Nature Protocols
Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) uses an amphiphilic peptide reagent to mediate intracellular delivery of CRISPR enzymes for high-efficiency editing of stimulated immune cells and cultured hematopoietic stem and progenitor cells (HSPCs).
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March 12, 2025 at 10:54 AM
#NewNProt for Peptide-enabled #ribonucleoprotein delivery for #CRISPR engineering (PERC) for ex vivo #genomeediting of primary human cells bit.ly/3DdsrY0
#NewNProt for #genomewide characterization of a diverse set of #smallnoncodingRNAs in cells and tissues
Optimized identification and characterization of small RNAs with PANDORA-seq - Nature Protocols
This Protocol describes optimized procedures for purification and sequencing of small noncoding RNAs from a variety of samples to characterize these diverse RNA species and map them to the parental RNAs from which they originate.
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April 8, 2025 at 10:48 AM
#NewNProt for #genomewide characterization of a diverse set of #smallnoncodingRNAs in cells and tissues
#NewNProt details the fabrication of thin-film #neuralimplants, small, flexible electronic devices less than ten micrometers in thickness bit.ly/3CUSFhX #bioelectronics #neuroscience @GeorgeMalliaras @AlexanderJBoys
Fabrication of thin-film electrodes and organic electrochemical transistors for neural implants - Nature Protocols
The fabrication of thin-film poly(ethylene dioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) electrodes and electrochemical transistors via photolithography enables the reliable construction of mech...
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February 26, 2025 at 10:44 AM
#NewNProt details the fabrication of thin-film #neuralimplants, small, flexible electronic devices less than ten micrometers in thickness bit.ly/3CUSFhX #bioelectronics #neuroscience @GeorgeMalliaras @AlexanderJBoys
#NewNProt: Measuring #plasmamembrane fluidity using #confocalmicroscopy bit.ly/4bie557 #membranefluidity
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
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March 5, 2025 at 8:33 AM
#NewNProt: Measuring #plasmamembrane fluidity using #confocalmicroscopy bit.ly/4bie557 #membranefluidity
#NewNProt: Structural connectome atlases using #MRtrix3 software to process #dMRI data bit.ly/4hdlMuZ
Structural connectome construction using constrained spherical deconvolution in multi-shell diffusion-weighted magnetic resonance imaging - Nature Protocols
This is a comprehensive protocol for the analysis of white matter diffusion-weighted magnetic resonance imaging data, which enables the representation of neuroanatomical atlases by using network-based...
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February 26, 2025 at 1:51 PM
#NewNProt: Structural connectome atlases using #MRtrix3 software to process #dMRI data bit.ly/4hdlMuZ
#NewNProt: #Synthesis of bicyclo[3.1.1]heptanes, meta-substituted arene isosteres, from [3.1.1]propellane bit.ly/4ibZ0Ez
Synthesis of bicyclo[3.1.1]heptanes, meta-substituted arene isosteres, from [3.1.1]propellane - Nature Protocols
Replacing benzene rings with small-ring bridged hydrocarbons can improve a drug’s physicochemical and pharmacokinetic profile. This protocol describes the synthesis of the potential meta-arene isoster...
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March 4, 2025 at 7:09 AM
#NewNProt: #Synthesis of bicyclo[3.1.1]heptanes, meta-substituted arene isosteres, from [3.1.1]propellane bit.ly/4ibZ0Ez
#NewNProt: Sonication-assisted #proteinextraction improves proteomic detection of membrane-bound and #DNAbindingproteins from tumor tissues bit.ly/438vGub
Sonication-assisted protein extraction improves proteomic detection of membrane-bound and DNA-binding proteins from tumor tissues - Nature Protocols
Mass spectrometry-based proteomic studies involve tissue lysis. Herein, we updated our 2018 protocol1 by implementing a sonication step into urea-based tissue lysis to improve the detection of membran...
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February 27, 2025 at 9:00 AM
#NewNProt: Sonication-assisted #proteinextraction improves proteomic detection of membrane-bound and #DNAbindingproteins from tumor tissues bit.ly/438vGub
#NewNProt: Protocol Update of #CellPhoneDB, describing an expanded database of molecular interactors and new approaches to infer, prioritise and visualise the results bit.ly/4lbG2Af and see associated News & Views article
CellPhoneDB v5: inferring cell–cell communication from single-cell multiomics data - Nature Protocols
CellPhoneDB combines a curated database of receptor–ligand interactions with methods to infer cell–cell communication from single-cell expression data. This protocol update describes an expanded datab...
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April 1, 2025 at 1:02 PM
#NewNProt: Protocol Update of #CellPhoneDB, describing an expanded database of molecular interactors and new approaches to infer, prioritise and visualise the results bit.ly/4lbG2Af and see associated News & Views article
#NewNProt for how to combine #STEDmicroscopy of #nanostructures with #functionalimaging in live settings. Find all you need to know for performing #superresolution #STED including troubleshooting solutions! Check it out bit.ly/4bXv7Wt
Live STED imaging of functional neuroanatomy - Nature Protocols
Super-resolution microscopy using a confocal STED microscope enables analyzing the relationship between morphological and functional neuronal structures at nanoscale resolution in living brain slices.
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March 19, 2025 at 6:40 PM
#NewNProt for how to combine #STEDmicroscopy of #nanostructures with #functionalimaging in live settings. Find all you need to know for performing #superresolution #STED including troubleshooting solutions! Check it out bit.ly/4bXv7Wt
#NewNProt: DARLIN mouse for in vivo #lineagetracing at high efficiency and clonal diversity bit.ly/4hFeoZm
DARLIN mouse for in vivo lineage tracing at high efficiency and clonal diversity - Nature Protocols
The DARLIN mouse enables the study of the cell lineages of millions of cells and at a high efficiency in vivo, through the incorporation of three barcoding arrays and a Cas9–TdT to improve editing div...
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March 26, 2025 at 10:56 AM
#NewNProt: DARLIN mouse for in vivo #lineagetracing at high efficiency and clonal diversity bit.ly/4hFeoZm
#NewNProt for evaluation and error correction of #genomeassemblies based on #longreadsequencing data bit.ly/4chEHnp
A detailed guide to assessing genome assembly based on long-read sequencing data using Inspector - Nature Protocols
This protocol describes a versatile computational tool for reference-free or reference-guided assessment and correction of errors in de novo genome assembly based on long-read sequencing data, showcas...
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April 2, 2025 at 7:02 AM
#NewNProt for evaluation and error correction of #genomeassemblies based on #longreadsequencing data bit.ly/4chEHnp
#NewNProt for standardising the evaluation of #multiphotonmicroscope performance; the art of multiphoton microscope maintenance bit.ly/3FEDeLF
Standardized measurements for monitoring and comparing multiphoton microscope systems - Nature Protocols
A set of procedures is described here for standardizing the evaluation of multiphoton microscope performance, covering laser power, pulse width optimizations, field of view, resolution and photomultip...
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March 20, 2025 at 9:53 AM
#NewNProt for standardising the evaluation of #multiphotonmicroscope performance; the art of multiphoton microscope maintenance bit.ly/3FEDeLF
#NewNProt: Controlled culturing and selection of #Chlamydomonas strains with differing characteristics, tailored for a variety of applications bit.ly/41wdEjj
Preparation, maintenance and propagation of synchronous cultures of photoactive Chlamydomonas cells - Nature Protocols
Catalan et al. present a protocol covering the selection and characterization of experimental Chlamydomonas strains driven by specific applications, where culture synchronicity, population growth rate...
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March 19, 2025 at 2:06 PM
#NewNProt: Controlled culturing and selection of #Chlamydomonas strains with differing characteristics, tailored for a variety of applications bit.ly/41wdEjj
#NewNProt for generation of #photoreceptor progenitors from #humanpluripotentstemcells under xeno-free and chemically defined conditions bit.ly/3F6Nqwd
Retina-specific laminin-based generation of photoreceptor progenitors from human pluripotent stem cells under xeno-free and chemically defined conditions - Nature Protocols
This protocol describes the generation of photoreceptor progenitors from human pluripotent stem cells under xeno-free and chemically defined conditions, as well as steps to produce the retina-specific...
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March 6, 2025 at 3:55 PM
#NewNProt for generation of #photoreceptor progenitors from #humanpluripotentstemcells under xeno-free and chemically defined conditions bit.ly/3F6Nqwd