#FluorescenceFriday chick neural tube section stained for PAX7 (magenta) and SOX2 (yellow)
November 29, 2024 at 8:16 PM
#FluorescenceFriday chick neural tube section stained for PAX7 (magenta) and SOX2 (yellow)
TF binding is usually anticorrelated with DNA methylation, but there are very few studies that actually investigate why. This is really nice work from Jacques Drouin's lab showing PAX7 physically interacts with UHFR1, blocking DNMT1 recruitment @ircm.bsky.social www.science.org/doi/10.1126/...
Dual DNA demethylation mechanisms implement epigenetic memory driven by the pioneer factor PAX7
Pioneer factor PAX7 highjacks control of DNA methylation maintenance, leading to enhancer demethylation and epigenetic memory.
www.science.org
May 21, 2025 at 1:04 PM
TF binding is usually anticorrelated with DNA methylation, but there are very few studies that actually investigate why. This is really nice work from Jacques Drouin's lab showing PAX7 physically interacts with UHFR1, blocking DNMT1 recruitment @ircm.bsky.social www.science.org/doi/10.1126/...
Good to see this study from Ribes & co in print: PAX3/PAX7 transcription factors control neural tube patterning by simultaneously repressing ventral fates via H3K27me3 deposition at silencers & activating dorsal fates as pioneer factors at enhancers
@ribes
journals.plos.org/plosbiology/...
@ribes
journals.plos.org/plosbiology/...
Dual transcriptional activities of PAX3 and PAX7 spatially encode spinal cell fates through distinct gene networks
How do transcription factors, with pleotropic functions, generate organized cellular diversity in developing tissues? This study shows that PAX3 and PAX7 orchestrate spinal cord patterning by acting a...
journals.plos.org
October 29, 2025 at 3:21 PM
Good to see this study from Ribes & co in print: PAX3/PAX7 transcription factors control neural tube patterning by simultaneously repressing ventral fates via H3K27me3 deposition at silencers & activating dorsal fates as pioneer factors at enhancers
@ribes
journals.plos.org/plosbiology/...
@ribes
journals.plos.org/plosbiology/...
✨ A developmental biology classic! Somitogenesis of a chick embryo 🐣 is lighting up in this fluorescent HCR staining. The notochord (Chordin 🟡) and somites (PAX7 💎) are labelled. 🔬
📸 credit: Tatiane Kanno
#FluorescenceFriday #devbio
📸 credit: Tatiane Kanno
#FluorescenceFriday #devbio
May 8, 2026 at 6:04 PM
✨ A developmental biology classic! Somitogenesis of a chick embryo 🐣 is lighting up in this fluorescent HCR staining. The notochord (Chordin 🟡) and somites (PAX7 💎) are labelled. 🔬
📸 credit: Tatiane Kanno
#FluorescenceFriday #devbio
📸 credit: Tatiane Kanno
#FluorescenceFriday #devbio
#FluorescenceFriday for my first post, of course. Developing chicken embryo transfected with pCIG on the left (green) and pCI H2B-RFP on the right (red) and co-stained for SOX10 (magenta) and PAX7 (cyan).
November 23, 2024 at 1:46 AM
#FluorescenceFriday for my first post, of course. Developing chicken embryo transfected with pCIG on the left (green) and pCI H2B-RFP on the right (red) and co-stained for SOX10 (magenta) and PAX7 (cyan).
A semi‐automated pipeline for quantitation of Pax7+, myonuclei, and cross‐sectional area by fiber type - Megowan - 2026 - Physiological Reports - Wiley Online Library physoc.onlinelibrary.wiley.com/doi/10.14814...
A semi‐automated pipeline for quantitation of Pax7+, myonuclei, and cross‐sectional area by fiber type
Created in BioRender. Megowan, H. (2026) https://BioRender.com/9457v5e.
physoc.onlinelibrary.wiley.com
September 27, 2026 at 2:42 PM
A semi‐automated pipeline for quantitation of Pax7+, myonuclei, and cross‐sectional area by fiber type - Megowan - 2026 - Physiological Reports - Wiley Online Library physoc.onlinelibrary.wiley.com/doi/10.14814...
Happy #FluorescenceFriday! This is the developing neural tube of an E9.5 mouse. We can't use it for analysis because the Nkx2.2 antibody (magenta) was causing some drama - but still a pretty picture! Am I the only one who sees Gonzo here? 🧪 👩🔬 #DevBio image by Lab Manager Miriam Dillard.
November 8, 2024 at 6:35 PM
Happy #FluorescenceFriday! This is the developing neural tube of an E9.5 mouse. We can't use it for analysis because the Nkx2.2 antibody (magenta) was causing some drama - but still a pretty picture! Am I the only one who sees Gonzo here? 🧪 👩🔬 #DevBio image by Lab Manager Miriam Dillard.
✍️ The @spinalorga.bsky.social lab published a new article
📖 @plosbiology.org
📃 Dual transcriptional activities of PAX3 and PAX7 spatially encode spinal cell fates through distinct gene networks
🔗 journals.plos.org/plosbiology/...
📖 @plosbiology.org
📃 Dual transcriptional activities of PAX3 and PAX7 spatially encode spinal cell fates through distinct gene networks
🔗 journals.plos.org/plosbiology/...
October 28, 2025 at 3:40 PM
✍️ The @spinalorga.bsky.social lab published a new article
📖 @plosbiology.org
📃 Dual transcriptional activities of PAX3 and PAX7 spatially encode spinal cell fates through distinct gene networks
🔗 journals.plos.org/plosbiology/...
📖 @plosbiology.org
📃 Dual transcriptional activities of PAX3 and PAX7 spatially encode spinal cell fates through distinct gene networks
🔗 journals.plos.org/plosbiology/...
#DBfeature 🐣
CRISPR-Cas13d enables targeted gene expression knockdown in chick embryos, expanding the experimental potential and versatility of this model system.
by Minyoung Kim and Erica Hutchins @drericabiophd.bsky.social
www.sciencedirect.com/science/arti...
#CRISPRCas #ChickEmbryo
CRISPR-Cas13d enables targeted gene expression knockdown in chick embryos, expanding the experimental potential and versatility of this model system.
by Minyoung Kim and Erica Hutchins @drericabiophd.bsky.social
www.sciencedirect.com/science/arti...
#CRISPRCas #ChickEmbryo
August 12, 2025 at 1:13 PM
#DBfeature 🐣
CRISPR-Cas13d enables targeted gene expression knockdown in chick embryos, expanding the experimental potential and versatility of this model system.
by Minyoung Kim and Erica Hutchins @drericabiophd.bsky.social
www.sciencedirect.com/science/arti...
#CRISPRCas #ChickEmbryo
CRISPR-Cas13d enables targeted gene expression knockdown in chick embryos, expanding the experimental potential and versatility of this model system.
by Minyoung Kim and Erica Hutchins @drericabiophd.bsky.social
www.sciencedirect.com/science/arti...
#CRISPRCas #ChickEmbryo
#SETDB1 #EndogenousRetroVirus
Pax7+ #MuSC, but not Pdgfra+ FAP, SETDB1 KO🐭➡️defected #SkeletalMuscle Regeneration
50%⏬muscle mass
Loss of SETDB1-H3K9me3 in MuSC depresses ERV expression ▶️
⏫cGAC-STING-IRF3▶️
⏬proliferation ⏫cell death ⏫pro-inflamm cytokines
#DevCell 2025
www.cell.com/developmenta...
Pax7+ #MuSC, but not Pdgfra+ FAP, SETDB1 KO🐭➡️defected #SkeletalMuscle Regeneration
50%⏬muscle mass
Loss of SETDB1-H3K9me3 in MuSC depresses ERV expression ▶️
⏫cGAC-STING-IRF3▶️
⏬proliferation ⏫cell death ⏫pro-inflamm cytokines
#DevCell 2025
www.cell.com/developmenta...
May 1, 2025 at 11:28 AM
#SETDB1 #EndogenousRetroVirus
Pax7+ #MuSC, but not Pdgfra+ FAP, SETDB1 KO🐭➡️defected #SkeletalMuscle Regeneration
50%⏬muscle mass
Loss of SETDB1-H3K9me3 in MuSC depresses ERV expression ▶️
⏫cGAC-STING-IRF3▶️
⏬proliferation ⏫cell death ⏫pro-inflamm cytokines
#DevCell 2025
www.cell.com/developmenta...
Pax7+ #MuSC, but not Pdgfra+ FAP, SETDB1 KO🐭➡️defected #SkeletalMuscle Regeneration
50%⏬muscle mass
Loss of SETDB1-H3K9me3 in MuSC depresses ERV expression ▶️
⏫cGAC-STING-IRF3▶️
⏬proliferation ⏫cell death ⏫pro-inflamm cytokines
#DevCell 2025
www.cell.com/developmenta...
🔎 Spotted in #AdvancedScience!
Researchers from #NYUGrossman and @dnanexus.bsky.social used our PAX7 antibody in a study mapping FAP signaling in skeletal muscle, revealing how cell populations communicate during homeostasis and regeneration.
📄 tinyurl.com/32k25caf
🧫 dshb.biology.uiowa.edu/PAX7
Researchers from #NYUGrossman and @dnanexus.bsky.social used our PAX7 antibody in a study mapping FAP signaling in skeletal muscle, revealing how cell populations communicate during homeostasis and regeneration.
📄 tinyurl.com/32k25caf
🧫 dshb.biology.uiowa.edu/PAX7
June 4, 2026 at 10:06 PM
🔎 Spotted in #AdvancedScience!
Researchers from #NYUGrossman and @dnanexus.bsky.social used our PAX7 antibody in a study mapping FAP signaling in skeletal muscle, revealing how cell populations communicate during homeostasis and regeneration.
📄 tinyurl.com/32k25caf
🧫 dshb.biology.uiowa.edu/PAX7
Researchers from #NYUGrossman and @dnanexus.bsky.social used our PAX7 antibody in a study mapping FAP signaling in skeletal muscle, revealing how cell populations communicate during homeostasis and regeneration.
📄 tinyurl.com/32k25caf
🧫 dshb.biology.uiowa.edu/PAX7
🥳 Our preprint is LIVE 🥳 Huge thanks to Lex Tallan and the Stanton lab for another fantastic collaboration! Here, we use zebrafish and MNase-based methodology to show that PAX3::FOXO1 and PAX7::FOXO1 have divergent capabilities for interacting with repressive chromatin states and nucleosomal DNA.
Comparative modes of chromatin engagement by PAX::FOXO1 fusions in rhabdomyosarcoma https://www.biorxiv.org/content/10.64898/2026.02.18.706582v1
February 23, 2026 at 6:35 PM
🥳 Our preprint is LIVE 🥳 Huge thanks to Lex Tallan and the Stanton lab for another fantastic collaboration! Here, we use zebrafish and MNase-based methodology to show that PAX3::FOXO1 and PAX7::FOXO1 have divergent capabilities for interacting with repressive chromatin states and nucleosomal DNA.
#FibroAdipogenicProgenitor #FAP
Pdgfra+ FAP IGF-1 KO🐭
👉No baseline #SkeletalMuscle anomalies in 1 mo
👉⏬FAP proliferation ⏫FAP #Senescence⏬Pax7+ MuSc⏬Myofiber size in BaCl2 SkM injury
👉Impaired fibrotic AND adipogenic capacities of FAP in vivo
#MyoSky
bioRxiv 2025
www.biorxiv.org/content/10.1...
Pdgfra+ FAP IGF-1 KO🐭
👉No baseline #SkeletalMuscle anomalies in 1 mo
👉⏬FAP proliferation ⏫FAP #Senescence⏬Pax7+ MuSc⏬Myofiber size in BaCl2 SkM injury
👉Impaired fibrotic AND adipogenic capacities of FAP in vivo
#MyoSky
bioRxiv 2025
www.biorxiv.org/content/10.1...
May 9, 2025 at 11:22 AM
#FibroAdipogenicProgenitor #FAP
Pdgfra+ FAP IGF-1 KO🐭
👉No baseline #SkeletalMuscle anomalies in 1 mo
👉⏬FAP proliferation ⏫FAP #Senescence⏬Pax7+ MuSc⏬Myofiber size in BaCl2 SkM injury
👉Impaired fibrotic AND adipogenic capacities of FAP in vivo
#MyoSky
bioRxiv 2025
www.biorxiv.org/content/10.1...
Pdgfra+ FAP IGF-1 KO🐭
👉No baseline #SkeletalMuscle anomalies in 1 mo
👉⏬FAP proliferation ⏫FAP #Senescence⏬Pax7+ MuSc⏬Myofiber size in BaCl2 SkM injury
👉Impaired fibrotic AND adipogenic capacities of FAP in vivo
#MyoSky
bioRxiv 2025
www.biorxiv.org/content/10.1...
New #Dev_Cell paper from @RelaixLab #Morikis #LeGrandLab1 and colleagues: Postnatal Pax7-expressing limb cells are multipotent and generate non-myogenic lineages that persist into adulthood. #Myoblue tinyurl.com/4btwerez
Postnatal Pax7-expressing limb cells are multipotent and generate non-myogenic lineages that persist into adulthood
Gioftsidi, Hayashi, et al. uncover that in mouse neonatal hindlimb muscles Pax7 progenitors
generate both myogenic and non-myogenic cells, including a specific subpopulation
of fibroblast-like cells, ...
tinyurl.com
August 27, 2026 at 6:43 PM
New #Dev_Cell paper from @RelaixLab #Morikis #LeGrandLab1 and colleagues: Postnatal Pax7-expressing limb cells are multipotent and generate non-myogenic lineages that persist into adulthood. #Myoblue tinyurl.com/4btwerez
📢 PhD Thesis defense
📅 Sept. 25th
📍 IJM
Robin Rondon ( @spinalorga.bsky.social Lab ) will defend his PhD thesis "De la répression à l’activation : le double jeu transcriptionnel de PAX3 et PAX7 au cours de la spécification des neurones de la moelle épinière sensorielle"
➡️ buff.ly/tF2oHmv
📅 Sept. 25th
📍 IJM
Robin Rondon ( @spinalorga.bsky.social Lab ) will defend his PhD thesis "De la répression à l’activation : le double jeu transcriptionnel de PAX3 et PAX7 au cours de la spécification des neurones de la moelle épinière sensorielle"
➡️ buff.ly/tF2oHmv
September 9, 2025 at 2:04 PM
📢 PhD Thesis defense
📅 Sept. 25th
📍 IJM
Robin Rondon ( @spinalorga.bsky.social Lab ) will defend his PhD thesis "De la répression à l’activation : le double jeu transcriptionnel de PAX3 et PAX7 au cours de la spécification des neurones de la moelle épinière sensorielle"
➡️ buff.ly/tF2oHmv
📅 Sept. 25th
📍 IJM
Robin Rondon ( @spinalorga.bsky.social Lab ) will defend his PhD thesis "De la répression à l’activation : le double jeu transcriptionnel de PAX3 et PAX7 au cours de la spécification des neurones de la moelle épinière sensorielle"
➡️ buff.ly/tF2oHmv
New #SkeletalMuscle paper: A Pax7::Foxo1 conditional mouse strain. #Myoblue tinyurl.com/sex3ec52 #aRMS
A Pax7::Foxo1 conditional mouse strain - Skeletal Muscle
Skeletal Muscle - The PAX7::FOXO1 subtype of alveolar rhabdomyosarcoma (aRMS) is both understudied and an unmet clinical need. The biology of PAX7::FOXO1 aRMS is significantly different than...
tinyurl.com
March 28, 2026 at 8:58 PM
New #SkeletalMuscle paper: A Pax7::Foxo1 conditional mouse strain. #Myoblue tinyurl.com/sex3ec52 #aRMS
A trippy video of the day to end a trippy year: www.youtube.com/watch?v=pAX7...
Star Trek- White Rabbit.flv
YouTube video by Darken6669
www.youtube.com
December 31, 2024 at 4:53 PM
A trippy video of the day to end a trippy year: www.youtube.com/watch?v=pAX7...
How do pleiotropic TFs generate organized diversity in developing tissues? @spinalorga.bsky.social shows that PAX3 & PAX7 organize #SpinalCord by acting as both repressors & pioneer activators, regulated by #morphogens to ensure precise neural subtype specification @plosbiology.org 🧪 plos.io/4qumsla
October 27, 2025 at 5:25 PM
How do pleiotropic TFs generate organized diversity in developing tissues? @spinalorga.bsky.social shows that PAX3 & PAX7 organize #SpinalCord by acting as both repressors & pioneer activators, regulated by #morphogens to ensure precise neural subtype specification @plosbiology.org 🧪 plos.io/4qumsla
New #stemcellreports paper: Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells. #Myoblue tinyurl.com/yyjhn94p
Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells
In this manuscript, Sacco, Caputo, and colleagues demonstrate that modulation of the
JAK2/STAT3 signaling pathway during in vitro myogenic differentiation promotes expansion
of PAX7+ SMPCs in multiple...
tinyurl.com
October 30, 2025 at 4:07 PM
New #stemcellreports paper: Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells. #Myoblue tinyurl.com/yyjhn94p
🔍 The Laryngoscope: NYU used anti-Pax7 clone PAX7 to study swallowing muscle after chemoradiation. Results point to impaired regeneration; the rat model showed no neuromuscular-junction change.
📄 https://tinyurl.com/36rdnaar
🧫https://dshb.biology.uiowa.edu/PAX7
#AntibodiesForResearch
📄 https://tinyurl.com/36rdnaar
🧫https://dshb.biology.uiowa.edu/PAX7
#AntibodiesForResearch
September 21, 2026 at 7:15 PM
🔍 The Laryngoscope: NYU used anti-Pax7 clone PAX7 to study swallowing muscle after chemoradiation. Results point to impaired regeneration; the rat model showed no neuromuscular-junction change.
📄 https://tinyurl.com/36rdnaar
🧫https://dshb.biology.uiowa.edu/PAX7
#AntibodiesForResearch
📄 https://tinyurl.com/36rdnaar
🧫https://dshb.biology.uiowa.edu/PAX7
#AntibodiesForResearch
Hey #MyoBlue: if you are looking for an alternative to DSHB Pax7 to use on sections, try mCadherin from CST (#40491) - it's gorgeous (and it's rabbit)!
November 22, 2024 at 8:14 PM
Hey #MyoBlue: if you are looking for an alternative to DSHB Pax7 to use on sections, try mCadherin from CST (#40491) - it's gorgeous (and it's rabbit)!
Toutes nos félicitations à Juliette Harris qui a soutenu sa thèse de doctorat intitulée «Mémoire épigénétique et transcriptionnelle de l’action pionnière de PAX7.» Bravo!! 👏
Elle était supervisée par le Dr Jacques Drouin, directeur de l'Unité de recherche en génétique moléculaire.
Elle était supervisée par le Dr Jacques Drouin, directeur de l'Unité de recherche en génétique moléculaire.
June 16, 2025 at 10:15 AM
Toutes nos félicitations à Juliette Harris qui a soutenu sa thèse de doctorat intitulée «Mémoire épigénétique et transcriptionnelle de l’action pionnière de PAX7.» Bravo!! 👏
Elle était supervisée par le Dr Jacques Drouin, directeur de l'Unité de recherche en génétique moléculaire.
Elle était supervisée par le Dr Jacques Drouin, directeur de l'Unité de recherche en génétique moléculaire.
Second co-corrisponding preprint is out! In collaboration with Alessandra Sacco group, we manage to double the yield of PAX7+ SMPCs generated in vitro and to mature them to a postnatal stage by modulation JAK2/STAT3 signaling.
Thanks to CIRM and MDA for their support
#myosky #myogenesis #DMD
Thanks to CIRM and MDA for their support
#myosky #myogenesis #DMD
Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSCs-derived myogenic progenitor cells https://www.biorxiv.org/content/10.1101/2024.12.09.624203v1
December 10, 2024 at 11:02 AM
Second co-corrisponding preprint is out! In collaboration with Alessandra Sacco group, we manage to double the yield of PAX7+ SMPCs generated in vitro and to mature them to a postnatal stage by modulation JAK2/STAT3 signaling.
Thanks to CIRM and MDA for their support
#myosky #myogenesis #DMD
Thanks to CIRM and MDA for their support
#myosky #myogenesis #DMD
How do pleiotropic TFs generate organized diversity in developing tissues? @spinalorga.bsky.social shows that PAX3 & PAX7 organize #SpinalCord by acting as both repressors & pioneer activators, regulated by #morphogens to ensure precise neural subtype specification @plosbiology.org 🧪 plos.io/4qumsla
October 27, 2025 at 9:55 AM
How do pleiotropic TFs generate organized diversity in developing tissues? @spinalorga.bsky.social shows that PAX3 & PAX7 organize #SpinalCord by acting as both repressors & pioneer activators, regulated by #morphogens to ensure precise neural subtype specification @plosbiology.org 🧪 plos.io/4qumsla
🔎 Spotted in @natcomms.nature.com!
Researchers from Li Ka Shing Institute of Health Sciences, among others, used DSHB's anti-Pax7 [PAX7] mAb in a study on cellular senescence profiling of aging human skeletal muscle leading to a potential treatment for sarcopenia.
Researchers from Li Ka Shing Institute of Health Sciences, among others, used DSHB's anti-Pax7 [PAX7] mAb in a study on cellular senescence profiling of aging human skeletal muscle leading to a potential treatment for sarcopenia.
July 14, 2025 at 7:05 PM
🔎 Spotted in @natcomms.nature.com!
Researchers from Li Ka Shing Institute of Health Sciences, among others, used DSHB's anti-Pax7 [PAX7] mAb in a study on cellular senescence profiling of aging human skeletal muscle leading to a potential treatment for sarcopenia.
Researchers from Li Ka Shing Institute of Health Sciences, among others, used DSHB's anti-Pax7 [PAX7] mAb in a study on cellular senescence profiling of aging human skeletal muscle leading to a potential treatment for sarcopenia.