Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast
The work from Alejandro Melero @ameleroc.bsky.social and Miguel Basante-Bedoya just out in Current Biology.
www.sciencedirect.com/science/arti...
@biology-unige.bsky.social
The work from Alejandro Melero @ameleroc.bsky.social and Miguel Basante-Bedoya just out in Current Biology.
www.sciencedirect.com/science/arti...
@biology-unige.bsky.social
Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast
Endosomes serve as crucial sorting centers that streamline the distribution of cell surface proteins. The early endosome receives traffic from both th…
www.sciencedirect.com
March 17, 2026 at 8:55 PM
Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast
The work from Alejandro Melero @ameleroc.bsky.social and Miguel Basante-Bedoya just out in Current Biology.
www.sciencedirect.com/science/arti...
@biology-unige.bsky.social
The work from Alejandro Melero @ameleroc.bsky.social and Miguel Basante-Bedoya just out in Current Biology.
www.sciencedirect.com/science/arti...
@biology-unige.bsky.social
For this #FluorescenceFriday, some endosomes imaged with MI-SIM. Green: Rab21, Magenta: F-actin, Cyan: EPLINalpha.
May 29, 2026 at 12:38 PM
For this #FluorescenceFriday, some endosomes imaged with MI-SIM. Green: Rab21, Magenta: F-actin, Cyan: EPLINalpha.
More Nikon NSPARC confocal testing at the @ncis-marseille.bsky.social: super-resolved live-cell imaging (1 frame every 10 seconds) of a COS-7 cell labeled for DNA (blue), endosomes (pink) and actin (gray) using probes from @spirochrome.com
March 5, 2025 at 3:35 PM
More Nikon NSPARC confocal testing at the @ncis-marseille.bsky.social: super-resolved live-cell imaging (1 frame every 10 seconds) of a COS-7 cell labeled for DNA (blue), endosomes (pink) and actin (gray) using probes from @spirochrome.com
New work @harvard by Miguel Gonzalez-Lozano @harperlabhms.bsky.social & @ernstschmid.bsky.social in Johannes Walter lab charts structural interactome of endosomes. #XL-MS #Alphafold Funded by @asapresearch.parkinsonsroadmap.org & NIH. Science continues-despite attacks www.nature.com/articles/s41...
EndoMAP.v1 charts the structural landscape of human early endosome complexes - Nature
A study presents EndoMAP.v1, a resource that combines information on protein interactions and crosslink-supported structural predictions to map the interaction landscape of early endosomes.
www.nature.com
May 28, 2025 at 3:52 PM
New work @harvard by Miguel Gonzalez-Lozano @harperlabhms.bsky.social & @ernstschmid.bsky.social in Johannes Walter lab charts structural interactome of endosomes. #XL-MS #Alphafold Funded by @asapresearch.parkinsonsroadmap.org & NIH. Science continues-despite attacks www.nature.com/articles/s41...
go.bsky.app/2zGz8ot
Autophagy Starter Pack - for those interested in autophagy (macro, micro and CMA), lysosomes and late endosomes! Let’s join hands to degrade the trash 😃
Autophagy Starter Pack - for those interested in autophagy (macro, micro and CMA), lysosomes and late endosomes! Let’s join hands to degrade the trash 😃
November 19, 2024 at 11:07 PM
go.bsky.app/2zGz8ot
Autophagy Starter Pack - for those interested in autophagy (macro, micro and CMA), lysosomes and late endosomes! Let’s join hands to degrade the trash 😃
Autophagy Starter Pack - for those interested in autophagy (macro, micro and CMA), lysosomes and late endosomes! Let’s join hands to degrade the trash 😃
Wonder doesn’t flinch. It’s our compass pulling us toward curiosity, creativity It reminds us: #science & #discovery are good. Here’s a moment that stirred mine: a new PI(3,4)P₂ probe illuminating the cell membrane & endosomes. Marvel with me. Captured on #FluorescenceFriday for #MicroscopyMonday
May 26, 2025 at 4:43 PM
Wonder doesn’t flinch. It’s our compass pulling us toward curiosity, creativity It reminds us: #science & #discovery are good. Here’s a moment that stirred mine: a new PI(3,4)P₂ probe illuminating the cell membrane & endosomes. Marvel with me. Captured on #FluorescenceFriday for #MicroscopyMonday
In issue 6:
Commentary: organoids for decoding host-microbe interactions
Importance of mitochondrial DNA elimination during spermatogenesis
How kinesin KIF12 promotes steatohepatitis
Cancer cell adaptation strategies to aneuploidy
Cover: Daniel Ott, Anne Spang et al
www.embopress.org/toc/14602075...
Commentary: organoids for decoding host-microbe interactions
Importance of mitochondrial DNA elimination during spermatogenesis
How kinesin KIF12 promotes steatohepatitis
Cancer cell adaptation strategies to aneuploidy
Cover: Daniel Ott, Anne Spang et al
www.embopress.org/toc/14602075...
March 17, 2025 at 3:18 PM
🔬🔦In their Imaging spotlight, @taylashakespeare.bsky.social and @ijayas.bsky.social highlight their research dissecting receptor sorting in endosomes using the calibrated 3D ExM toolkit that they developed.
focalplane.biologists.com/2026/04/15/i...
focalplane.biologists.com/2026/04/15/i...
Imaging spotlight: Calibrating expansion microscopy to map receptor sorting domains in endosomes - FocalPlane
Imaging spotlight: Calibrating expansion microscopy to map receptor sorting domains in endosomes - News
focalplane.biologists.com
April 20, 2026 at 2:20 PM
🔬🔦In their Imaging spotlight, @taylashakespeare.bsky.social and @ijayas.bsky.social highlight their research dissecting receptor sorting in endosomes using the calibrated 3D ExM toolkit that they developed.
focalplane.biologists.com/2026/04/15/i...
focalplane.biologists.com/2026/04/15/i...
Beautiful imaging on the cover but also check out the beautiful imaging inside and our story on #endosomes and #GTPase cascades
👋🏻Our November issue's out!
Cover: Imaging #Ovulation
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
Cover: Imaging #Ovulation
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
November 20, 2024 at 6:36 PM
Beautiful imaging on the cover but also check out the beautiful imaging inside and our story on #endosomes and #GTPase cascades
Phosphoinositide lipids may be the link between lumen pH to compartment targeting of endosomes and phagosomes. #Science 🧪
Congratulations to former Rao Lab postdoc Kalyan Kondapalli on his exciting molecular insights linking endosomal pH to phagosome trafficking!
Read here: umdearborn.edu/news/ph-gps-...
Read here: umdearborn.edu/news/ph-gps-...
January 6, 2025 at 8:50 PM
Phosphoinositide lipids may be the link between lumen pH to compartment targeting of endosomes and phagosomes. #Science 🧪
I’m not going to be able to tell you about the consequences of EVERY lost grant, but I’m going to try.
Let’s start with the Antiviral Drug Discovery (AViDD) program for pathogens of pandemic concern! It just got unceremoniously shitcanned.
Let’s start with the Antiviral Drug Discovery (AViDD) program for pathogens of pandemic concern! It just got unceremoniously shitcanned.
March 27, 2025 at 5:06 PM
I’m not going to be able to tell you about the consequences of EVERY lost grant, but I’m going to try.
Let’s start with the Antiviral Drug Discovery (AViDD) program for pathogens of pandemic concern! It just got unceremoniously shitcanned.
Let’s start with the Antiviral Drug Discovery (AViDD) program for pathogens of pandemic concern! It just got unceremoniously shitcanned.
👋🏻Our November issue's out!
Cover: Imaging #Ovulation
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
Cover: Imaging #Ovulation
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
November 20, 2024 at 5:28 PM
👋🏻Our November issue's out!
Cover: Imaging #Ovulation
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
Cover: Imaging #Ovulation
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #PlasmaMembrane #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
Nothing better for procrastination than going through some live cell imaging of Endosomes...
Today we have the fusion of two equally sized PI(3)P positive endosomes. This is at 5s framerate, wish I had taken it at higher temporal resolution but still super cool to see
Today we have the fusion of two equally sized PI(3)P positive endosomes. This is at 5s framerate, wish I had taken it at higher temporal resolution but still super cool to see
May 29, 2026 at 1:27 PM
Nothing better for procrastination than going through some live cell imaging of Endosomes...
Today we have the fusion of two equally sized PI(3)P positive endosomes. This is at 5s framerate, wish I had taken it at higher temporal resolution but still super cool to see
Today we have the fusion of two equally sized PI(3)P positive endosomes. This is at 5s framerate, wish I had taken it at higher temporal resolution but still super cool to see
Tremendous effort and great native organellar interactome resource from Miguel and co in @harperlabhms.bsky.social : a crosslinking-MS, co-fractionation, and alphaFold interactome on isolated EEA1+ endosomes (+discovery/validation!) | www.biorxiv.org/content/10.1...
EndoMAPV1, a Structural Protein Complex Landscape of Human Endosomes
Early/sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome1,2,3. These events are c...
www.biorxiv.org
February 11, 2025 at 11:38 PM
Tremendous effort and great native organellar interactome resource from Miguel and co in @harperlabhms.bsky.social : a crosslinking-MS, co-fractionation, and alphaFold interactome on isolated EEA1+ endosomes (+discovery/validation!) | www.biorxiv.org/content/10.1...
The architecture of carriers formed by AP3:ARF1 for lysosomal membrane protein transport: www.science.org/doi/10.1126/.... Work from @jggkaufman.bsky.social, in collaboration with @dgershlick.bsky.social and David Owen. @mpibiochem.bsky.social @thecimr.bsky.social
Architecture of clathrin-independent AP3:ARF1-coated carriers
AP3 generates clathrin-free carriers on endosomes by organizing via ARF1 dimers into elongated membrane-deforming arrays.
www.science.org
May 18, 2026 at 11:24 AM
The architecture of carriers formed by AP3:ARF1 for lysosomal membrane protein transport: www.science.org/doi/10.1126/.... Work from @jggkaufman.bsky.social, in collaboration with @dgershlick.bsky.social and David Owen. @mpibiochem.bsky.social @thecimr.bsky.social
New study from our lab at NICHD, NIH! We identify TBC1D9 and TBC1D9B as novel ARL8 effectors that suppress exosome secretion by inactivating RAB11A and thus blocking exocyst-dependent fusion of multivesicular endosomes with the plasma membrane. www.pnas.org/doi/10.1073/...
July 23, 2026 at 6:47 PM
New study from our lab at NICHD, NIH! We identify TBC1D9 and TBC1D9B as novel ARL8 effectors that suppress exosome secretion by inactivating RAB11A and thus blocking exocyst-dependent fusion of multivesicular endosomes with the plasma membrane. www.pnas.org/doi/10.1073/...
Starting to write up the first mechanistic paper from my lab about regulation of endosomal PI(3)P. We see retromer tubules (Vps35) budding from PI(3)P positive sorting endosomes with Actin at the neck. When PI(3)P homeostasis is perturbed this organisation breaks down and cells cant recycle cargo.
June 11, 2026 at 5:13 PM
Starting to write up the first mechanistic paper from my lab about regulation of endosomal PI(3)P. We see retromer tubules (Vps35) budding from PI(3)P positive sorting endosomes with Actin at the neck. When PI(3)P homeostasis is perturbed this organisation breaks down and cells cant recycle cargo.
Most protein complexes are born only once. V-ATPase assembles over and over as part of its regulatory mechanism, enabled by RAVE (regulator of ATPase of vacuoles and endosomes)
@hanlinw222.bsky.social's 1st struct of RAVE bound to a partial V1 complex now out in PNAS.
www.pnas.org/doi/10.1073/...
@hanlinw222.bsky.social's 1st struct of RAVE bound to a partial V1 complex now out in PNAS.
www.pnas.org/doi/10.1073/...
December 3, 2024 at 7:34 PM
Most protein complexes are born only once. V-ATPase assembles over and over as part of its regulatory mechanism, enabled by RAVE (regulator of ATPase of vacuoles and endosomes)
@hanlinw222.bsky.social's 1st struct of RAVE bound to a partial V1 complex now out in PNAS.
www.pnas.org/doi/10.1073/...
@hanlinw222.bsky.social's 1st struct of RAVE bound to a partial V1 complex now out in PNAS.
www.pnas.org/doi/10.1073/...
Happy Fluorescence Friday! Our #CellLineOfTheWeek is Rab-5a - illuminating endosomes! 🧫
Our stem cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery.
📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social
#OpenScience
Our stem cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery.
📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social
#OpenScience
July 18, 2025 at 4:00 PM
Happy Fluorescence Friday! Our #CellLineOfTheWeek is Rab-5a - illuminating endosomes! 🧫
Our stem cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery.
📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social
#OpenScience
Our stem cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery.
📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social
#OpenScience
☕Our November issue's out folks!
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #ovulation #imaging #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
@natureportfolio.bsky.social
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #ovulation #imaging #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
@natureportfolio.bsky.social
November 18, 2024 at 1:25 PM
☕Our November issue's out folks!
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #ovulation #imaging #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
@natureportfolio.bsky.social
👉🏼Comments on #embryo models & #MembraneContactSites
🔬on: #ovulation #imaging #endosomes #CellDeath #metabolism #mTOR #BiomolecularCondensation #gastruloids #organoids #ProteinQualityControl & more!
www.nature.com/ncb/volumes/...
@natureportfolio.bsky.social
"It's official 🎉: Our remote magnetic control of #endosomes, driving invasive protrusions in living cells, has landed in #ScienceAdvances! #Magnetogenetics meets #Rabs! The journey was wild, but it works! 🔥 www.science.org/doi/full/10....
@imgprague.bsky.social #3Dmatrix #CellBiology
@science.org
@imgprague.bsky.social #3Dmatrix #CellBiology
@science.org
Live-cell magnetic manipulation of recycling endosomes reveals their direct effect on actin protrusions to promote invasive migration
Repositioning of Rab25 vesicles containing formin and integrin cargos to the cell periphery promotes protrusion formation.
www.science.org
July 7, 2025 at 12:58 PM
"It's official 🎉: Our remote magnetic control of #endosomes, driving invasive protrusions in living cells, has landed in #ScienceAdvances! #Magnetogenetics meets #Rabs! The journey was wild, but it works! 🔥 www.science.org/doi/full/10....
@imgprague.bsky.social #3Dmatrix #CellBiology
@science.org
@imgprague.bsky.social #3Dmatrix #CellBiology
@science.org
How do Endosomes dance? Looks like a waltz? Or maybe the more of a cèilidh?
Cells expressing the PI(3)P probe imaged every 5s for ~15min. Currently preparing a really exciting paper about the regulation of endosomal PI(3)P homeostasis. Stay tuned!
Cells expressing the PI(3)P probe imaged every 5s for ~15min. Currently preparing a really exciting paper about the regulation of endosomal PI(3)P homeostasis. Stay tuned!
May 9, 2026 at 11:14 AM
How do Endosomes dance? Looks like a waltz? Or maybe the more of a cèilidh?
Cells expressing the PI(3)P probe imaged every 5s for ~15min. Currently preparing a really exciting paper about the regulation of endosomal PI(3)P homeostasis. Stay tuned!
Cells expressing the PI(3)P probe imaged every 5s for ~15min. Currently preparing a really exciting paper about the regulation of endosomal PI(3)P homeostasis. Stay tuned!
Very excited for what promises to be a full immersion into #endosomes next year!
Excited to announce, the 93rd Biochemical Society Harden Conference will be on "Endosomal-lysosomal network dysfunction in neurodegeneration". Come and join us in early September on the beautiful south coast of England. Lots of speaker slots available from submitted abstracts!
November 20, 2025 at 4:40 PM
Very excited for what promises to be a full immersion into #endosomes next year!
Great opportunity for a Research Support Officer to join @cellbiol-mrclmb.bsky.social, supporting @deriverylab.bsky.social in their studies of the molecular mechanisms behind receptor sorting in endosomes.
More info: www.nature.com/naturecareer...
Apply by 10 JUL
#ScienceJobs
More info: www.nature.com/naturecareer...
Apply by 10 JUL
#ScienceJobs
June 23, 2025 at 3:11 PM
Great opportunity for a Research Support Officer to join @cellbiol-mrclmb.bsky.social, supporting @deriverylab.bsky.social in their studies of the molecular mechanisms behind receptor sorting in endosomes.
More info: www.nature.com/naturecareer...
Apply by 10 JUL
#ScienceJobs
More info: www.nature.com/naturecareer...
Apply by 10 JUL
#ScienceJobs