New month, new method: What is flow cytometry?
It is a powerful technique used for hundreds of applications, including clinical diagnostics, basic research, and discovery.
#FlowCytometry #WIMMResearch #CellAnalysis #AcademicSky #CellSorting @rdm.ox.ac.uk @medsci.ox.ac.uk #Oxford
New month, new method: What is flow cytometry?
It is a powerful technique used for hundreds of applications, including clinical diagnostics, basic research, and discovery.
#FlowCytometry #WIMMResearch #CellAnalysis #AcademicSky #CellSorting @rdm.ox.ac.uk @medsci.ox.ac.uk #Oxford
Here we go!
Ever wondered how scientists can measure dozens of proteins on a single cell? #MassCytometry makes this possible!
Here we go!
Ever wondered how scientists can measure dozens of proteins on a single cell? #MassCytometry makes this possible!
How do we edit #DNA?
At the Genome Engineering Facility, DNA editing is mainly carried out using CRISPR Cas9 technology for targeted mutagenesis in vivo.
#CRISPR #GenomeEngineering #GeneEditing #WIMMResearch #AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
How do we edit #DNA?
At the Genome Engineering Facility, DNA editing is mainly carried out using CRISPR Cas9 technology for targeted mutagenesis in vivo.
#CRISPR #GenomeEngineering #GeneEditing #WIMMResearch #AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
So… how does #MassCytometry actually work? 🤔
1. Cells are stained with antibodies tagged to rare heavy metals
2. Each cell is vaporised and ionised in a plasma
3. Ions are separated by their mass and detected in a time-of-flight (TOF) mass spectrometer
So… how does #MassCytometry actually work? 🤔
1. Cells are stained with antibodies tagged to rare heavy metals
2. Each cell is vaporised and ionised in a plasma
3. Ions are separated by their mass and detected in a time-of-flight (TOF) mass spectrometer
Our cytometry facility supports hundreds of researchers each year, from training to troubleshooting and advanced sorting. This week, we highlight the team behind the facility, whose expertise makes this work possible.
Our cytometry facility supports hundreds of researchers each year, from training to troubleshooting and advanced sorting. This week, we highlight the team behind the facility, whose expertise makes this work possible.
What is #imaging in biomedical research?
Imaging means a diverse set of methods to visualise and quantify biological structures, specimen and their function.
@medsci.ox.ac.uk
@rdm.ox.ac.uk
What is #imaging in biomedical research?
Imaging means a diverse set of methods to visualise and quantify biological structures, specimen and their function.
@medsci.ox.ac.uk
@rdm.ox.ac.uk
How does #FlowCytometry work?
1. Cells pass single file through laser beams.
2. Lasers excite markers and scatter light linked to cell features.
3. Detectors measure the signals.
Together, this builds a detailed profile of each cell.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
How does #FlowCytometry work?
1. Cells pass single file through laser beams.
2. Lasers excite markers and scatter light linked to cell features.
3. Detectors measure the signals.
Together, this builds a detailed profile of each cell.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
What makes #MassCytometry such a powerful tool for the #MRCWIMM researchers?
#WIMMResearch #Immunology #Hematology #SingleCellScience
@medsci.ox.ac.uk @drakesmith-lab.bsky.social 🧬
What makes #MassCytometry such a powerful tool for the #MRCWIMM researchers?
#WIMMResearch #Immunology #Hematology #SingleCellScience
@medsci.ox.ac.uk @drakesmith-lab.bsky.social 🧬
Behind every powerful technique is a team of dedicated scientists.
Meet the MRC WIMM Mass Cytometry Facility, where our mass cytometer helps researchers explore biology at single-cell resolution.
#WIMMResearch #MassCytometry #CoreFacilities #ScienceBehindTheScenes #MeetTheTeam
Behind every powerful technique is a team of dedicated scientists.
Meet the MRC WIMM Mass Cytometry Facility, where our mass cytometer helps researchers explore biology at single-cell resolution.
#WIMMResearch #MassCytometry #CoreFacilities #ScienceBehindTheScenes #MeetTheTeam
Aristotle's test for whether a scene belongs: "a thing whose presence or absence makes no visible difference, is not an organic part of the whole."
Which of your scenes survives that?
https://www.gutenberg.org/ebooks/1974
#writesky #MethodMonday
Aristotle's test for whether a scene belongs: "a thing whose presence or absence makes no visible difference, is not an organic part of the whole."
Which of your scenes survives that?
https://www.gutenberg.org/ebooks/1974
#writesky #MethodMonday
How is #imaging used in research at the #MRCWIMM?
Scientists choose microscopes to image specimens and to measure, track, and record changes during development, disease progression, or drug treatment.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
How is #imaging used in research at the #MRCWIMM?
Scientists choose microscopes to image specimens and to measure, track, and record changes during development, disease progression, or drug treatment.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
Meet the #Imaging Facility at the #MRCWIMM
With over 15 years of expertise each, our two MRC WIMM imaging specialists maintain the instruments and support researchers working with a diverse range of research questions and imaging demands.
@medsci.ox.ac.uk @rdm.ox.ac.uk #AcademicSky
Meet the #Imaging Facility at the #MRCWIMM
With over 15 years of expertise each, our two MRC WIMM imaging specialists maintain the instruments and support researchers working with a diverse range of research questions and imaging demands.
@medsci.ox.ac.uk @rdm.ox.ac.uk #AcademicSky
Editing DNA is only part of the story. The editing machinery must first reach the cell.
#GeneDelivery #CRISPR #GenomeEditing #WIMMResearch #AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
Editing DNA is only part of the story. The editing machinery must first reach the cell.
#GeneDelivery #CRISPR #GenomeEditing #WIMMResearch #AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
New month, new method: #GenomeEditing
How do we edit #DNA?
The Genome Engineering Facility mainly applies CRISPR Cas9 technology for targeted mutagenesis in vivo.
@rdm.ox.ac.uk @medsci.ox.ac.uk #AcademicSky
New month, new method: #GenomeEditing
How do we edit #DNA?
The Genome Engineering Facility mainly applies CRISPR Cas9 technology for targeted mutagenesis in vivo.
@rdm.ox.ac.uk @medsci.ox.ac.uk #AcademicSky
Great to see 100+ researchers come together to share ideas and connect.
Read more below ⬇️
www.imm.ox.ac.uk/news/OGEF-2026
#MethodMonday 🧪 #AcademicSky @medsci.ox.ac.uk @rdm.ox.ac.uk @ox.ac.uk
Great to see 100+ researchers come together to share ideas and connect.
Read more below ⬇️
www.imm.ox.ac.uk/news/OGEF-2026
#MethodMonday 🧪 #AcademicSky @medsci.ox.ac.uk @rdm.ox.ac.uk @ox.ac.uk
#MethodMonday
#MethodMonday
How does imaging work?
There are many types of light microscopes, from basic widefield and epifluorescence systems familiar from school laboratories, to advanced techniques such as laser scanning confocal, spinning disc confocal, and super resolution microscopy.
How does imaging work?
There are many types of light microscopes, from basic widefield and epifluorescence systems familiar from school laboratories, to advanced techniques such as laser scanning confocal, spinning disc confocal, and super resolution microscopy.
Behind the scenes of single cell OMICS at the #MRCWIMM
The Advanced Single Cell OMICS Facility provides end to end support for single cell, bulk RNAseq, and spatial transcriptomics and proteomics projects, from sample preparation to sequencing and analysis.
#AcademicSky
Behind the scenes of single cell OMICS at the #MRCWIMM
The Advanced Single Cell OMICS Facility provides end to end support for single cell, bulk RNAseq, and spatial transcriptomics and proteomics projects, from sample preparation to sequencing and analysis.
#AcademicSky
How does single cell OMICS work?
1. Cells or nuclei are isolated from tissues and QC.
2. Individual cells are captured and barcoded.
3. RNA, DNA, or proteins are analysed separately for each cell.
@rdm.ox.ac.uk @medsci.ox.ac.uk #AcademicSky #SingleCell #scRNAseq #Multiomics
How does single cell OMICS work?
1. Cells or nuclei are isolated from tissues and QC.
2. Individual cells are captured and barcoded.
3. RNA, DNA, or proteins are analysed separately for each cell.
@rdm.ox.ac.uk @medsci.ox.ac.uk #AcademicSky #SingleCell #scRNAseq #Multiomics
It's always nice to see information on research become more accessible to the public (and to the researchers & institutions who are curious about others' methodologies 😜)
It's always nice to see information on research become more accessible to the public (and to the researchers & institutions who are curious about others' methodologies 😜)
How does HPC work for us?
1️⃣ Data is securely uploaded to our petabyte-scale storage system
2️⃣ Jobs are queued and dispatched to multiple compute servers
3️⃣ Each job is processed with the requested resources (CPU, GPU, RAM, etc)
4️⃣ Results and trail records are made available
How does HPC work for us?
1️⃣ Data is securely uploaded to our petabyte-scale storage system
2️⃣ Jobs are queued and dispatched to multiple compute servers
3️⃣ Each job is processed with the requested resources (CPU, GPU, RAM, etc)
4️⃣ Results and trail records are made available
Freytag's Pyramid, from the 1863 book everyone cites and nobody reads. He was analysing five-act tragedy, not novels. And he hedged: the shape holds "if one may symbolize its arrangement by lines."
https://archive.org/details/freytagstechniqu00freyuoft
#MethodMonday #writesky
Freytag's Pyramid, from the 1863 book everyone cites and nobody reads. He was analysing five-act tragedy, not novels. And he hedged: the shape holds "if one may symbolize its arrangement by lines."
https://archive.org/details/freytagstechniqu00freyuoft
#MethodMonday #writesky
Dan Harmon's story circle, in his own words. A character in comfort wants something, enters an unfamiliar situation, adapts, gets it, pays a heavy price, returns changed.
https://channel101.fandom.com/wiki/Story_Structure_101:_Super_Basic_Shit
#MethodMonday #writesky
Dan Harmon's story circle, in his own words. A character in comfort wants something, enters an unfamiliar situation, adapts, gets it, pays a heavy price, returns changed.
https://channel101.fandom.com/wiki/Story_Structure_101:_Super_Basic_Shit
#MethodMonday #writesky
What do we use single cell OMICS for?
The facility supports a wide range of technologies and applications, including: 👉 Continued in the comments.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
What do we use single cell OMICS for?
The facility supports a wide range of technologies and applications, including: 👉 Continued in the comments.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk