#methodmonday
Explaining why "woke" is a good thing to one person and an insult to another. On this #methodmonday we dive in #SymbolicInteractionism #sociology
April 21, 2025 at 8:30 AM
#MethodMonday 🧪

New month, new method: What is flow cytometry?

It is a powerful technique used for hundreds of applications, including clinical diagnostics, basic research, and discovery.

#FlowCytometry #WIMMResearch #CellAnalysis #AcademicSky #CellSorting @rdm.ox.ac.uk @medsci.ox.ac.uk #Oxford
March 2, 2026 at 9:49 AM
#MethodMonday From now on, each week, we’ll highlight a different research method used at the #MRCWIMM, breaking down how it works and why it’s important. 🧪

Here we go!

Ever wondered how scientists can measure dozens of proteins on a single cell? #MassCytometry makes this possible!
January 5, 2026 at 11:10 AM
#MethodMonday 🧪
How do we edit #DNA?

At the Genome Engineering Facility, DNA editing is mainly carried out using CRISPR Cas9 technology for targeted mutagenesis in vivo.

#CRISPR #GenomeEngineering #GeneEditing #WIMMResearch #AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
April 20, 2026 at 9:44 AM
#MethodMonday 🧪

So… how does #MassCytometry actually work? 🤔

1. Cells are stained with antibodies tagged to rare heavy metals
2. Each cell is vaporised and ionised in a plasma
3. Ions are separated by their mass and detected in a time-of-flight (TOF) mass spectrometer
January 12, 2026 at 10:26 AM
#MethodMonday: Behind the scenes of #flowcytometry at the #MRCWIMM 🧪

Our cytometry facility supports hundreds of researchers each year, from training to troubleshooting and advanced sorting. This week, we highlight the team behind the facility, whose expertise makes this work possible.
March 23, 2026 at 9:34 AM
#MethodMonday 🧪

What is #imaging in biomedical research?

Imaging means a diverse set of methods to visualise and quantify biological structures, specimen and their function.

@medsci.ox.ac.uk
@rdm.ox.ac.uk
February 2, 2026 at 11:54 AM
#MethodMonday 🧪
How does #FlowCytometry work?

1. Cells pass single file through laser beams.
2. Lasers excite markers and scatter light linked to cell features.
3. Detectors measure the signals.

Together, this builds a detailed profile of each cell.
#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
March 9, 2026 at 11:32 AM
January 19, 2026 at 9:54 AM
#MethodMonday 🧪

Behind every powerful technique is a team of dedicated scientists.

Meet the MRC WIMM Mass Cytometry Facility, where our mass cytometer helps researchers explore biology at single-cell resolution.

#WIMMResearch #MassCytometry #CoreFacilities #ScienceBehindTheScenes #MeetTheTeam
January 26, 2026 at 11:07 AM
Method Monday.

Aristotle's test for whether a scene belongs: "a thing whose presence or absence makes no visible difference, is not an organic part of the whole."

Which of your scenes survives that?

https://www.gutenberg.org/ebooks/1974

#writesky #MethodMonday
The Poetics of Aristotle by Aristotle
Free eBook digitized and proofread by volunteers.
www.gutenberg.org
September 7, 2026 at 4:46 PM
#MethodMonday 🧪:
How is #imaging used in research at the #MRCWIMM?

Scientists choose microscopes to image specimens and to measure, track, and record changes during development, disease progression, or drug treatment.

#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
February 16, 2026 at 9:49 AM
#MethodMonday 🧪
Meet the #Imaging Facility at the #MRCWIMM
With over 15 years of expertise each, our two MRC WIMM imaging specialists maintain the instruments and support researchers working with a diverse range of research questions and imaging demands.

@medsci.ox.ac.uk @rdm.ox.ac.uk #AcademicSky
February 23, 2026 at 9:52 AM
#MethodMonday🧪

Editing DNA is only part of the story. The editing machinery must first reach the cell.

#GeneDelivery #CRISPR #GenomeEditing #WIMMResearch #AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
April 27, 2026 at 9:28 AM
#MethodMonday 🧪
New month, new method: #GenomeEditing
How do we edit #DNA?
The Genome Engineering Facility mainly applies CRISPR Cas9 technology for targeted mutagenesis in vivo.

@rdm.ox.ac.uk @medsci.ox.ac.uk #AcademicSky
April 13, 2026 at 8:51 AM
On Thursday we hosted the 2026 Genome Engineering Forum at #MRCWIMM

Great to see 100+ researchers come together to share ideas and connect.

Read more below ⬇️
www.imm.ox.ac.uk/news/OGEF-2026
#MethodMonday 🧪 #AcademicSky @medsci.ox.ac.uk @rdm.ox.ac.uk @ox.ac.uk
2026 Genome Engineering Forum brings together Oxford research community
The Genome Engineering, Virus Production and Transgenics Facility at the MRC Weatherall Institute of Molecular Medicine (WIMM), together with the MRC Mary Lyon Centre, recently organised the 2026 Geno...
www.imm.ox.ac.uk
April 20, 2026 at 1:32 PM
Permanent birth control includes tubal ligation for those with ovaries and vasectomy for those with testicles, both over 99% effective. They are one-time procedures, not easily reversible, and safe, with vasectomy typically having a quicker recovery.
#MethodMonday
October 6, 2025 at 6:02 PM
#MethodMonday 🧪
How does imaging work?

There are many types of light microscopes, from basic widefield and epifluorescence systems familiar from school laboratories, to advanced techniques such as laser scanning confocal, spinning disc confocal, and super resolution microscopy.
February 9, 2026 at 10:33 AM
#MethodMonday 🧪

How does single cell OMICS work?

1. Cells or nuclei are isolated from tissues and QC.

2. Individual cells are captured and barcoded.

3. RNA, DNA, or proteins are analysed separately for each cell.

@rdm.ox.ac.uk @medsci.ox.ac.uk #AcademicSky #SingleCell #scRNAseq #Multiomics
June 15, 2026 at 8:38 AM
#MethodMonday 🧪

Behind the scenes of single cell OMICS at the #MRCWIMM

The Advanced Single Cell OMICS Facility provides end to end support for single cell, bulk RNAseq, and spatial transcriptomics and proteomics projects, from sample preparation to sequencing and analysis.
#AcademicSky
June 29, 2026 at 8:32 AM
Just stumbled upon one of the later #MethodMonday posts of yours, and I have to say, it's a very interesting idea!

It's always nice to see information on research become more accessible to the public (and to the researchers & institutions who are curious about others' methodologies 😜)
January 19, 2026 at 12:35 PM
#MethodMonday 🧪
How does HPC work for us?

1️⃣ Data is securely uploaded to our petabyte-scale storage system
2️⃣ Jobs are queued and dispatched to multiple compute servers
3️⃣ Each job is processed with the requested resources (CPU, GPU, RAM, etc)
4️⃣ Results and trail records are made available
May 18, 2026 at 8:40 AM
Method Monday.

Freytag's Pyramid, from the 1863 book everyone cites and nobody reads. He was analysing five-act tragedy, not novels. And he hedged: the shape holds "if one may symbolize its arrangement by lines."

https://archive.org/details/freytagstechniqu00freyuoft

#MethodMonday #writesky
August 17, 2026 at 5:35 PM
Method Monday.

Dan Harmon's story circle, in his own words. A character in comfort wants something, enters an unfamiliar situation, adapts, gets it, pays a heavy price, returns changed.

https://channel101.fandom.com/wiki/Story_Structure_101:_Super_Basic_Shit

#MethodMonday #writesky
August 10, 2026 at 5:43 PM
#MethodMonday 🧬

What do we use single cell OMICS for?

The facility supports a wide range of technologies and applications, including: 👉 Continued in the comments.

#AcademicSky @rdm.ox.ac.uk @medsci.ox.ac.uk
June 22, 2026 at 8:32 AM