Shahid Mansoor’s team reviewed the evolution of plant #primeediting from PE1 to AI-designed PE8, highlighting pegRNA stabilization, cis-regulatory expression tuning, and PE-recombinase systems for predictive crop breeding. 🌾🤖 #PlantBiotech2026 #TrendsInPlantScience #CropGenomics #AIinBiotech
September 23, 2026 at 4:51 AM
Shahid Mansoor’s team reviewed the evolution of plant #primeediting from PE1 to AI-designed PE8, highlighting pegRNA stabilization, cis-regulatory expression tuning, and PE-recombinase systems for predictive crop breeding. 🌾🤖 #PlantBiotech2026 #TrendsInPlantScience #CropGenomics #AIinBiotech
CRISPR technologies for genome, epigenome & transcriptome editing
Dazzling ever-expanding Cas-verse😎
#BaseEditing
#PrimeEditing
Large insertions/deletion kb
Transient/Stable transcriptional activation/inhibition
RNA degradation/editing/methylation🤠
#NatRevMCB 2024
www.nature.com/articles/s41...
Dazzling ever-expanding Cas-verse😎
#BaseEditing
#PrimeEditing
Large insertions/deletion kb
Transient/Stable transcriptional activation/inhibition
RNA degradation/editing/methylation🤠
#NatRevMCB 2024
www.nature.com/articles/s41...
February 14, 2024 at 8:25 PM
CRISPR technologies for genome, epigenome & transcriptome editing
Dazzling ever-expanding Cas-verse😎
#BaseEditing
#PrimeEditing
Large insertions/deletion kb
Transient/Stable transcriptional activation/inhibition
RNA degradation/editing/methylation🤠
#NatRevMCB 2024
www.nature.com/articles/s41...
Dazzling ever-expanding Cas-verse😎
#BaseEditing
#PrimeEditing
Large insertions/deletion kb
Transient/Stable transcriptional activation/inhibition
RNA degradation/editing/methylation🤠
#NatRevMCB 2024
www.nature.com/articles/s41...
Coming up in September!
➡️Christina Kajba @ckajba.bsky.social & Michael Herger @michaelherger.bsky.social — pooled prime editing screens in haploid human cells
➡️Jon Acosta — multiplexed in vivo base editing screens
#GeneticVariants #PrimeEditing
ℹ️https://www.varianteffect.org/seminar-series/
➡️Christina Kajba @ckajba.bsky.social & Michael Herger @michaelherger.bsky.social — pooled prime editing screens in haploid human cells
➡️Jon Acosta — multiplexed in vivo base editing screens
#GeneticVariants #PrimeEditing
ℹ️https://www.varianteffect.org/seminar-series/
August 7, 2025 at 2:26 PM
Coming up in September!
➡️Christina Kajba @ckajba.bsky.social & Michael Herger @michaelherger.bsky.social — pooled prime editing screens in haploid human cells
➡️Jon Acosta — multiplexed in vivo base editing screens
#GeneticVariants #PrimeEditing
ℹ️https://www.varianteffect.org/seminar-series/
➡️Christina Kajba @ckajba.bsky.social & Michael Herger @michaelherger.bsky.social — pooled prime editing screens in haploid human cells
➡️Jon Acosta — multiplexed in vivo base editing screens
#GeneticVariants #PrimeEditing
ℹ️https://www.varianteffect.org/seminar-series/
Partnership between David Liu, @jacksonlab.bsky.social, and rare disease group RARE Hope leads to first use of prime editing for a brain disease that reduced symptoms, extended lifespan in mice, offering hope for other rare genetic diseases. #Science #PrimeEditing
Prime editing treats childhood brain disease in mice
Scientists use a precise form of gene editing called prime editing to correct the most common genetic mutations that cause alternating hemiplegia of childhood, a rare and severe neurological disorder ...
www.broadinstitute.org
July 21, 2025 at 3:52 PM
Partnership between David Liu, @jacksonlab.bsky.social, and rare disease group RARE Hope leads to first use of prime editing for a brain disease that reduced symptoms, extended lifespan in mice, offering hope for other rare genetic diseases. #Science #PrimeEditing
So happy to have worked with Dr. Elie Haddad to help cure CGD using prime editing technology. An extraordinary moment for precision medicine and for children with rare immune disorders.
#CGD #PrimeEditing #PrecisionMedicine
#CGD #PrimeEditing #PrecisionMedicine
Our latest article features Dr. Stuart Turvey and Dr. Elie Haddad on how collaboration, clinical trials, precision health, and gene editing are helping transform pediatric rare disease research and access to treatment.
www.rarekidscan.com/news/rewriti...
www.rarekidscan.com/news/rewriti...
Rewriting Rare Disease Care: How Collaboration and Clinical Trials Are Transforming Pediatric Research and Access to Treatment — RareKids-CAN
Canada is entering a new era in pediatric rare disease research, driven by advances in precision medicine and novel, innovative clinical trial approaches, including gene editing and gene therapies.
www.rarekidscan.com
May 25, 2026 at 7:19 PM
So happy to have worked with Dr. Elie Haddad to help cure CGD using prime editing technology. An extraordinary moment for precision medicine and for children with rare immune disorders.
#CGD #PrimeEditing #PrecisionMedicine
#CGD #PrimeEditing #PrecisionMedicine
First patient receiving cells edited with prime editing, to treat chronic granulomatous disease! 💪🏻
#CRISPR #PrimeEditing
#CRISPR #PrimeEditing
World first: ultra-powerful CRISPR treatment trialled in a person
Immune-cell function improved in a teenager whose DNA was altered using prime editing.
buff.ly
May 30, 2025 at 11:43 AM
First patient receiving cells edited with prime editing, to treat chronic granulomatous disease! 💪🏻
#CRISPR #PrimeEditing
#CRISPR #PrimeEditing
🧬 CRISPR Meets Probiotics🦠
What if we could engineer probiotics with precision to fight disease and promote animal and plant health?
Read here-
🔗https://journals.asm.org/doi/10.1128/aem.00031-25
#CRISPR #PrimeEditing #MicrobiomeEngineering #Probiotics #SyntheticBiology #PrecisionMedicine
What if we could engineer probiotics with precision to fight disease and promote animal and plant health?
Read here-
🔗https://journals.asm.org/doi/10.1128/aem.00031-25
#CRISPR #PrimeEditing #MicrobiomeEngineering #Probiotics #SyntheticBiology #PrecisionMedicine
March 9, 2025 at 11:16 PM
🧬 CRISPR Meets Probiotics🦠
What if we could engineer probiotics with precision to fight disease and promote animal and plant health?
Read here-
🔗https://journals.asm.org/doi/10.1128/aem.00031-25
#CRISPR #PrimeEditing #MicrobiomeEngineering #Probiotics #SyntheticBiology #PrecisionMedicine
What if we could engineer probiotics with precision to fight disease and promote animal and plant health?
Read here-
🔗https://journals.asm.org/doi/10.1128/aem.00031-25
#CRISPR #PrimeEditing #MicrobiomeEngineering #Probiotics #SyntheticBiology #PrecisionMedicine
Chinese synthetic biologists engineered the PE7-scFv-MLH1dn system using SunTag spatial scaffolding to anchor mismatch repair inhibitors directly to active DNA nicks, lifting prime editing efficiencies by 68% without introducing background genomic damage. 🧬🤖#SynBio #CRISPR #PrimeEditing #SunTag
June 18, 2026 at 5:08 AM
Chinese synthetic biologists engineered the PE7-scFv-MLH1dn system using SunTag spatial scaffolding to anchor mismatch repair inhibitors directly to active DNA nicks, lifting prime editing efficiencies by 68% without introducing background genomic damage. 🧬🤖#SynBio #CRISPR #PrimeEditing #SunTag
TBC1D30 sequence & expression changes impact proinsulin & insulin secretion, linking this putative RabGAP to GWAS signal for proinsulin levels. Study suggests role for TBC1D30 in proinsulin secretion & diabetes risk. #T2D #Proinsulin #GWAS #PrimeEditing link.springer.com/article/10.1...
TBC1D30 regulates proinsulin and insulin secretion and is the target of a genomic association signal for proinsulin - Diabetologia
Aims/hypothesis Components of the insulin processing and secretion pathways remain incompletely understood. Here, we examined a genome-wide association study (GWAS) signal for plasma proinsulin levels...
link.springer.com
March 19, 2025 at 8:33 AM
TBC1D30 sequence & expression changes impact proinsulin & insulin secretion, linking this putative RabGAP to GWAS signal for proinsulin levels. Study suggests role for TBC1D30 in proinsulin secretion & diabetes risk. #T2D #Proinsulin #GWAS #PrimeEditing link.springer.com/article/10.1...
Epigenetics Update - Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing u.epigenome.us/fhjDiFSe
Daniel Price and Aliaksandra Radzisheuskaya (ICR, London) in natgenet.nature.com
#Epigenetics #CRISPR #Chromatin #PrimeEditing
---
epigenometech.com
Daniel Price and Aliaksandra Radzisheuskaya (ICR, London) in natgenet.nature.com
#Epigenetics #CRISPR #Chromatin #PrimeEditing
---
epigenometech.com
Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing - Nature Genetics
This study uses a precise and efficient clustered regularly interspaced short palindromic repeats (CRISPR) prime editing system to substitute lysine residues in histone H3, individually or in combinat...
u.epigenome.us
July 14, 2026 at 11:27 AM
Epigenetics Update - Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing u.epigenome.us/fhjDiFSe
Daniel Price and Aliaksandra Radzisheuskaya (ICR, London) in natgenet.nature.com
#Epigenetics #CRISPR #Chromatin #PrimeEditing
---
epigenometech.com
Daniel Price and Aliaksandra Radzisheuskaya (ICR, London) in natgenet.nature.com
#Epigenetics #CRISPR #Chromatin #PrimeEditing
---
epigenometech.com
Multimodal scanning of genetic variants with base and prime editing. #GeneticVariants #PrimeEditing #NatureBiotechnology
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Multimodal scanning of genetic variants with base and prime editing - Nature Biotechnology
Thousands of rare oncogene variants are evaluated using multimodal gene editing screens.
www.nature.com
November 12, 2024 at 11:12 PM
Multimodal scanning of genetic variants with base and prime editing. #GeneticVariants #PrimeEditing #NatureBiotechnology
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Scientists map cancer mutations in EGFR gene, revealing drug resistance paths 🔬🧬🧪 www.news-medical.net/news/2024111... #Cancer #GeneEditing #EGFR #PrecisionMedicine #DrugResistance #CancerProgression #BaseEditing #PrimeEditing #PersonalizedTreatment @naturebiotech.bsky.social
Scientists map cancer mutations in EGFR gene, revealing drug resistance paths
Researchers used base and prime editing to uncover new EGFR gene mutations that affect cancer progression and drug resistance, paving the way for personalized treatment strategies.
www.news-medical.net
November 14, 2024 at 10:46 PM
Scientists map cancer mutations in EGFR gene, revealing drug resistance paths 🔬🧬🧪 www.news-medical.net/news/2024111... #Cancer #GeneEditing #EGFR #PrecisionMedicine #DrugResistance #CancerProgression #BaseEditing #PrimeEditing #PersonalizedTreatment @naturebiotech.bsky.social
proPE splits tasks between two guides: engRNA nicks the DNA (exposing the non-target strand); tpgRNA carries PBS+RTT and binds nearby with a truncated spacer, delivering the template without cutting.
See schematics. #CRISPR #primeediting
See schematics. #CRISPR #primeediting
October 26, 2025 at 8:57 PM
proPE splits tasks between two guides: engRNA nicks the DNA (exposing the non-target strand); tpgRNA carries PBS+RTT and binds nearby with a truncated spacer, delivering the template without cutting.
See schematics. #CRISPR #primeediting
See schematics. #CRISPR #primeediting
ICR London scientists deployed high-throughput #CRISPR #primeediting across multicopy histone H3 gene arrays in stem cells, identifying critical essential lysines (H3K4, H3K9, H3K14, H3K18, H3K79) while demonstrating reversible editing and non-canonical H3.3 compensation dynamics. 🧬🤖
July 28, 2026 at 7:15 AM
ICR London scientists deployed high-throughput #CRISPR #primeediting across multicopy histone H3 gene arrays in stem cells, identifying critical essential lysines (H3K4, H3K9, H3K14, H3K18, H3K79) while demonstrating reversible editing and non-canonical H3.3 compensation dynamics. 🧬🤖
Henan Agricultural University researchers established TJ-PE in rice, enabling equal-length homologous promoter replacements up to 275 bp with 100% precision and multiplex deletions up to 2,024 bp. 🌾🤖 #PlantGenomics #PrimeEditing #TJPE #NewPhytologist2026 #RiceBreeding #AgBiotech
August 3, 2026 at 8:08 AM
Henan Agricultural University researchers established TJ-PE in rice, enabling equal-length homologous promoter replacements up to 275 bp with 100% precision and multiplex deletions up to 2,024 bp. 🌾🤖 #PlantGenomics #PrimeEditing #TJPE #NewPhytologist2026 #RiceBreeding #AgBiotech
🧬 QuadPE (Nature) uses four pegRNAs to insert up to 26 kb with ~40% efficiency — no DSBs, no recombinase — outperforming integrase systems by up to 61× and working in primary neurons and T cells. Yin/Zhang labs, Wuhan University. #PrimeEditing #GeneTherapy #CRISPR
May 20, 2026 at 3:36 AM
🧬 QuadPE (Nature) uses four pegRNAs to insert up to 26 kb with ~40% efficiency — no DSBs, no recombinase — outperforming integrase systems by up to 61× and working in primary neurons and T cells. Yin/Zhang labs, Wuhan University. #PrimeEditing #GeneTherapy #CRISPR
Researchers engineered flexiAsCas12a and the circular RNA-guided one-sniCPE2 split prime editor, expanding Cas12a PAM access to NATN/NCCN/GTCN motifs and raising theoretical human pathogenic SNV coverage to 95.86%. 🧬🤖
#PrimeEditing #Cas12a #flexiAsCas12a #GenomeBiology2026 #GeneTherapy #CRISPR
#PrimeEditing #Cas12a #flexiAsCas12a #GenomeBiology2026 #GeneTherapy #CRISPR
September 1, 2026 at 8:33 AM
Researchers engineered flexiAsCas12a and the circular RNA-guided one-sniCPE2 split prime editor, expanding Cas12a PAM access to NATN/NCCN/GTCN motifs and raising theoretical human pathogenic SNV coverage to 95.86%. 🧬🤖
#PrimeEditing #Cas12a #flexiAsCas12a #GenomeBiology2026 #GeneTherapy #CRISPR
#PrimeEditing #Cas12a #flexiAsCas12a #GenomeBiology2026 #GeneTherapy #CRISPR
🌿 Directly fusing Csy4 to the prime editor (uncleavable) instead of using a P2A linker reduces toxicity 13.5× in Arabidopsis and unlocks up to 85.7% T1 editing efficiency — decoupling Csy4's efficiency boost from its lethality in dicots. #PrimeEditing #Arabidopsis #CRISPR
May 25, 2026 at 5:18 AM
🌿 Directly fusing Csy4 to the prime editor (uncleavable) instead of using a P2A linker reduces toxicity 13.5× in Arabidopsis and unlocks up to 85.7% T1 editing efficiency — decoupling Csy4's efficiency boost from its lethality in dicots. #PrimeEditing #Arabidopsis #CRISPR
Prime-SGE: #saturation #mutagenesis via #primeediting
3825 epegRNAs → 1220 mutations in 7 cancer genes (EGFR, KRAS, BRAF, MET, MEK1, AKT, RIT1)
EGFR inhibitor screen: 17-37 resistance variants/drug. KRAS G12 = pan-resistance. C797S only in covalent inhibitors.
In vivo validated ✓
#CancerResearch
3825 epegRNAs → 1220 mutations in 7 cancer genes (EGFR, KRAS, BRAF, MET, MEK1, AKT, RIT1)
EGFR inhibitor screen: 17-37 resistance variants/drug. KRAS G12 = pan-resistance. C797S only in covalent inhibitors.
In vivo validated ✓
#CancerResearch
March 10, 2026 at 1:24 AM
Prime-SGE: #saturation #mutagenesis via #primeediting
3825 epegRNAs → 1220 mutations in 7 cancer genes (EGFR, KRAS, BRAF, MET, MEK1, AKT, RIT1)
EGFR inhibitor screen: 17-37 resistance variants/drug. KRAS G12 = pan-resistance. C797S only in covalent inhibitors.
In vivo validated ✓
#CancerResearch
3825 epegRNAs → 1220 mutations in 7 cancer genes (EGFR, KRAS, BRAF, MET, MEK1, AKT, RIT1)
EGFR inhibitor screen: 17-37 resistance variants/drug. KRAS G12 = pan-resistance. C797S only in covalent inhibitors.
In vivo validated ✓
#CancerResearch
🌾 First prime editing system in sorghum: fusing T5 exonuclease to nCas9+M-MLV (SbPEmaxT5E) boosted ALS W545L editing to 9.33% in protoplasts and 6.25% in stable plants — with transgene-free T1 lines confirmed herbicide-resistant in greenhouse trials. #PrimeEditing #Sorghum #CRISPR
May 20, 2026 at 8:33 AM
🌾 First prime editing system in sorghum: fusing T5 exonuclease to nCas9+M-MLV (SbPEmaxT5E) boosted ALS W545L editing to 9.33% in protoplasts and 6.25% in stable plants — with transgene-free T1 lines confirmed herbicide-resistant in greenhouse trials. #PrimeEditing #Sorghum #CRISPR
Fudan University researchers developed #DoPE, a donor-complementary #primeediting system that pairs opegRNAs with 3′-overhang dsDNA donors to achieve seamless 108 bp to 12.5 kb DNA insertions and high-throughput in situ saturation mutagenesis without DSBs. 🧬🤖 #CRISPR
August 31, 2026 at 9:38 AM
Fudan University researchers developed #DoPE, a donor-complementary #primeediting system that pairs opegRNAs with 3′-overhang dsDNA donors to achieve seamless 108 bp to 12.5 kb DNA insertions and high-throughput in situ saturation mutagenesis without DSBs. 🧬🤖 #CRISPR
Prime editing can potentially repair disease-causing human mutations, but the technology has not been widely used in vivo.
Now, scientists from the Liu lab at the @broadinstitute.org have addressed bottlenecks that impede the use of #PrimeEditing in patients: https://bit.ly/44qeLCJ
Now, scientists from the Liu lab at the @broadinstitute.org have addressed bottlenecks that impede the use of #PrimeEditing in patients: https://bit.ly/44qeLCJ
June 19, 2026 at 8:31 PM
Prime editing can potentially repair disease-causing human mutations, but the technology has not been widely used in vivo.
Now, scientists from the Liu lab at the @broadinstitute.org have addressed bottlenecks that impede the use of #PrimeEditing in patients: https://bit.ly/44qeLCJ
Now, scientists from the Liu lab at the @broadinstitute.org have addressed bottlenecks that impede the use of #PrimeEditing in patients: https://bit.ly/44qeLCJ
✂️ New in NAR: SPELL — a universal pegRNA design strategy that eliminates per-target PBS optimization for prime editing.
The SPELL solution: use a fixed long PBS (17–20 nt) + introduce a single 1 nt deletion at position ~13. That's it.
#PrimeEditing #CRISPR #pegRNA #GeneEditing #NAR
The SPELL solution: use a fixed long PBS (17–20 nt) + introduce a single 1 nt deletion at position ~13. That's it.
#PrimeEditing #CRISPR #pegRNA #GeneEditing #NAR
April 26, 2026 at 4:45 PM
✂️ New in NAR: SPELL — a universal pegRNA design strategy that eliminates per-target PBS optimization for prime editing.
The SPELL solution: use a fixed long PBS (17–20 nt) + introduce a single 1 nt deletion at position ~13. That's it.
#PrimeEditing #CRISPR #pegRNA #GeneEditing #NAR
The SPELL solution: use a fixed long PBS (17–20 nt) + introduce a single 1 nt deletion at position ~13. That's it.
#PrimeEditing #CRISPR #pegRNA #GeneEditing #NAR
First Curative Correction of a Deletion Mutation by #PrimeEditing (blog): rnaitherapeutics.blogspot.com/2025/05/firs...
$PRME $BEAM #CRISPR
$PRME $BEAM #CRISPR
First Curative Correction of a Deletion Mutation by Prime Editing
Another week, another disease cured by CRISPR technology. Today, Prime Medicine revealed that a young man with autosomal recessive chroni...
rnaitherapeutics.blogspot.com
May 19, 2025 at 7:03 PM
First Curative Correction of a Deletion Mutation by #PrimeEditing (blog): rnaitherapeutics.blogspot.com/2025/05/firs...
$PRME $BEAM #CRISPR
$PRME $BEAM #CRISPR
Prime Editing: The Next Frontier in Genetic Medicine www.news-medical.net/health/Prime... #PrimeEditing #GeneEditing #Genome #GeneticMedicine #CRISPR #PersonalizedMedicine #Biotechnology #Genetics #DNARepair
Prime Editing: The Next Frontier in Genetic Medicine
Prime editing is an advanced gene editing technology that enables precise DNA corrections without introducing double-stranded breaks, significantly reducing off-target effects. This innovation holds p...
www.news-medical.net
June 4, 2025 at 1:20 AM
Prime Editing: The Next Frontier in Genetic Medicine www.news-medical.net/health/Prime... #PrimeEditing #GeneEditing #Genome #GeneticMedicine #CRISPR #PersonalizedMedicine #Biotechnology #Genetics #DNARepair