New article of the lab on Notch3, not only involved in vascular or neural defects, but also in heart defects and laterality defects.
Creating an asymmetric heart: @tbonnely.bsky.social @audreydsg.bsky.social @meilhacsigolene.bsky.social &co identify Notch3 as a novel asymmetric factor in lateral plate mesoderm that acts as a genetic modifier of NODAL to regulate #heart #morphogenesis @plosbiology.org 🧪 plos.io/42q6H4L
April 1, 2025 at 7:55 PM
New article of the lab on Notch3, not only involved in vascular or neural defects, but also in heart defects and laterality defects.
Thrilled to share our latest research in Nature Leukemia led by our amazing postdoc Raúl Sánchez-Lanzas! This work adds new insights into the links between vascular dementia & blood disorders. Read more: rdcu.be/d0bi1 #stemcells #hematology #CADASIL #NOTCH3 🧪
A CADASIL NOTCH3 mutation leads to clonal hematopoiesis and expansion of Dnmt3a-R878H hematopoietic clones
Leukemia - A CADASIL NOTCH3 mutation leads to clonal hematopoiesis and expansion of Dnmt3a-R878H hematopoietic clones
rdcu.be
November 15, 2024 at 11:56 AM
Thrilled to share our latest research in Nature Leukemia led by our amazing postdoc Raúl Sánchez-Lanzas! This work adds new insights into the links between vascular dementia & blood disorders. Read more: rdcu.be/d0bi1 #stemcells #hematology #CADASIL #NOTCH3 🧪
#CADASIL #CerebralSmallVesselDisease
Great comparative phenomics &🧠vessel multiomics
1⃣scRNAseq 2⃣proteomics 3⃣phosphoproteomics
Pericyte vs Smooth Muscle Cell Notch3 R170C🐭
Surprisingly, Notch3 signaling per se was unaltered in either🐭
@rrust.bsky.social #CellRep 2026
www.cell.com/cell-reports...
Great comparative phenomics &🧠vessel multiomics
1⃣scRNAseq 2⃣proteomics 3⃣phosphoproteomics
Pericyte vs Smooth Muscle Cell Notch3 R170C🐭
Surprisingly, Notch3 signaling per se was unaltered in either🐭
@rrust.bsky.social #CellRep 2026
www.cell.com/cell-reports...
April 21, 2026 at 11:58 AM
#CADASIL #CerebralSmallVesselDisease
Great comparative phenomics &🧠vessel multiomics
1⃣scRNAseq 2⃣proteomics 3⃣phosphoproteomics
Pericyte vs Smooth Muscle Cell Notch3 R170C🐭
Surprisingly, Notch3 signaling per se was unaltered in either🐭
@rrust.bsky.social #CellRep 2026
www.cell.com/cell-reports...
Great comparative phenomics &🧠vessel multiomics
1⃣scRNAseq 2⃣proteomics 3⃣phosphoproteomics
Pericyte vs Smooth Muscle Cell Notch3 R170C🐭
Surprisingly, Notch3 signaling per se was unaltered in either🐭
@rrust.bsky.social #CellRep 2026
www.cell.com/cell-reports...
Online now: Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
Benamar et al. demonstrate that Notch3+ Treg cells are increased in multiple sclerosis and in EAE. They are induced in the gut by microbiota and translocate to the CNS to degenerate into pathogenic Th17 cells. They also outcompete a protective NPY1R+-resident Treg cell population that controls Th1 responses.
dlvr.it
October 2, 2025 at 7:56 PM
Online now: Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
@ganuzalab.bsky.social
Exciting new work from my former trainee, Mikel Ganuza, now running his own excellent team. Congrats!
#HSCs #clonalhematopoiesis
#science
www.nature.com/articles/s41...
Exciting new work from my former trainee, Mikel Ganuza, now running his own excellent team. Congrats!
#HSCs #clonalhematopoiesis
#science
www.nature.com/articles/s41...
A CADASIL NOTCH3 mutation leads to clonal hematopoiesis and expansion of Dnmt3a-R878H hematopoietic clones - Leukemia
Leukemia - A CADASIL NOTCH3 mutation leads to clonal hematopoiesis and expansion of Dnmt3a-R878H hematopoietic clones
www.nature.com
January 29, 2025 at 4:30 AM
@ganuzalab.bsky.social
Exciting new work from my former trainee, Mikel Ganuza, now running his own excellent team. Congrats!
#HSCs #clonalhematopoiesis
#science
www.nature.com/articles/s41...
Exciting new work from my former trainee, Mikel Ganuza, now running his own excellent team. Congrats!
#HSCs #clonalhematopoiesis
#science
www.nature.com/articles/s41...
#Microbiota #FriendOrFoe? Our own @bostonchildrens.bsky.social Chatila, Benamar &co show @cp-immunity.bsky.social that #gut microbiota drives Notch3+ Tregs which migrate to brain & interacts with microglia becoming Th17 cells, subvert CNS resident neuropetide-responsive Tregs, supporting MS/EAE!
Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
Benamar et al. demonstrate that Notch3+ Treg cells are increased in multiple sclerosis
and in EAE. They are induced in the gut by microbiota and translocate to the CNS to
degenerate into pathogenic Th...
www.cell.com
October 3, 2025 at 1:51 PM
#Microbiota #FriendOrFoe? Our own @bostonchildrens.bsky.social Chatila, Benamar &co show @cp-immunity.bsky.social that #gut microbiota drives Notch3+ Tregs which migrate to brain & interacts with microglia becoming Th17 cells, subvert CNS resident neuropetide-responsive Tregs, supporting MS/EAE!
Very happy to share our latest paper identifying Notch3 as a new left gene required for #heart #morphogenesis 💜
Many thanks to all the authors!
Many thanks to all the authors!
Creating an asymmetric heart: @tbonnely.bsky.social @audreydsg.bsky.social @meilhacsigolene.bsky.social &co identify Notch3 as a novel asymmetric factor in lateral plate mesoderm that acts as a genetic modifier of NODAL to regulate #heart #morphogenesis @plosbiology.org 🧪 plos.io/42q6H4L
April 2, 2025 at 12:27 PM
Very happy to share our latest paper identifying Notch3 as a new left gene required for #heart #morphogenesis 💜
Many thanks to all the authors!
Many thanks to all the authors!
Creating an asymmetric heart: @tbonnely.bsky.social @audreydsg.bsky.social @meilhacsigolene.bsky.social &co identify Notch3 as a novel asymmetric factor in lateral plate mesoderm that acts as a genetic modifier of NODAL to regulate #heart #morphogenesis @plosbiology.org 🧪 plos.io/42q6H4L
April 1, 2025 at 8:04 AM
Creating an asymmetric heart: @tbonnely.bsky.social @audreydsg.bsky.social @meilhacsigolene.bsky.social &co identify Notch3 as a novel asymmetric factor in lateral plate mesoderm that acts as a genetic modifier of NODAL to regulate #heart #morphogenesis @plosbiology.org 🧪 plos.io/42q6H4L
#WeekendRead! #FroendsOrFoes?! Benamar, Chatila &co identify @cp-immunity.bsky.social a #GutBrain axis leading to microbiota- & TLR-dependent induction of Notch3+ Tregs in the gut that in multiple sclerosis migrate to the CNS & interacting with microglia become pathogenic Th17 cells!
Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
Benamar et al. demonstrate that Notch3+ Treg cells are increased in multiple sclerosis
and in EAE. They are induced in the gut by microbiota and translocate to the CNS to
degenerate into pathogenic Th...
www.cell.com
November 29, 2025 at 5:31 PM
#WeekendRead! #FroendsOrFoes?! Benamar, Chatila &co identify @cp-immunity.bsky.social a #GutBrain axis leading to microbiota- & TLR-dependent induction of Notch3+ Tregs in the gut that in multiple sclerosis migrate to the CNS & interacting with microglia become pathogenic Th17 cells!
#Neurogenesis #Angiogenesis
scarb2a (enriched in Radial Glia Cell) -/- #Zebrafish
⏬endolysosomal acidification
⏬Notch3 processing
⏫Neurogenesis ⏬Gliogenesis
Altered Neural Paracrine vefgaa wnt7a
Perturbed vascular patterning & #BBB
Karina Yaniv lab #NatureComms 2024
www.nature.com/articles/s41...
scarb2a (enriched in Radial Glia Cell) -/- #Zebrafish
⏬endolysosomal acidification
⏬Notch3 processing
⏫Neurogenesis ⏬Gliogenesis
Altered Neural Paracrine vefgaa wnt7a
Perturbed vascular patterning & #BBB
Karina Yaniv lab #NatureComms 2024
www.nature.com/articles/s41...
November 3, 2024 at 12:19 PM
#Neurogenesis #Angiogenesis
scarb2a (enriched in Radial Glia Cell) -/- #Zebrafish
⏬endolysosomal acidification
⏬Notch3 processing
⏫Neurogenesis ⏬Gliogenesis
Altered Neural Paracrine vefgaa wnt7a
Perturbed vascular patterning & #BBB
Karina Yaniv lab #NatureComms 2024
www.nature.com/articles/s41...
scarb2a (enriched in Radial Glia Cell) -/- #Zebrafish
⏬endolysosomal acidification
⏬Notch3 processing
⏫Neurogenesis ⏬Gliogenesis
Altered Neural Paracrine vefgaa wnt7a
Perturbed vascular patterning & #BBB
Karina Yaniv lab #NatureComms 2024
www.nature.com/articles/s41...
The authors included RRIDs in their in Journal of Experimental & Clinical Cancer Research paper! We value the author's support of reproducibility. #accelerateopenscience #RRID #reproducibility
Prolyl-isomerase Pin1 drives platinum resistance by regulating Notch3 stability and function in ovarian cancer
Read the full paper: Prolyl-isomerase Pin1 drives platinum resistance by regulating Notch3 stability and function in ovarian cancer
doi.org
October 9, 2026 at 7:04 AM
The authors included RRIDs in their in Journal of Experimental & Clinical Cancer Research paper! We value the author's support of reproducibility. #accelerateopenscience #RRID #reproducibility
The authors included RRIDs in their in NAR Cancer paper! Thanks for making your methods matter! #reproducibility #ReproducibleResearch #RRID
LncRNA RAMS11-dependent NOTCH3 activation drives tumor progression in non-small cell lung cancer
Read the full paper: LncRNA RAMS11-dependent NOTCH3 activation drives tumor progression in non-small cell lung cancer
doi.org
October 8, 2026 at 7:00 AM
The authors included RRIDs in their in NAR Cancer paper! Thanks for making your methods matter! #reproducibility #ReproducibleResearch #RRID
CADASIL 3D Vessel-on-Chip models the effects of NOTCH3 R182C on Vasculopathy
AIM Biotech with primary or hiPSC-derived Smooth Muscle Cells
⏫SMC arborization surrounding endothelial tubes
⏫SMC NOTCH3-ECD, PDGFRβ, Contractile proteins, Ca2+ activity
#StemCellRep 2026
www.cell.com/stem-cell-re...
AIM Biotech with primary or hiPSC-derived Smooth Muscle Cells
⏫SMC arborization surrounding endothelial tubes
⏫SMC NOTCH3-ECD, PDGFRβ, Contractile proteins, Ca2+ activity
#StemCellRep 2026
www.cell.com/stem-cell-re...
April 2, 2026 at 11:47 AM
CADASIL 3D Vessel-on-Chip models the effects of NOTCH3 R182C on Vasculopathy
AIM Biotech with primary or hiPSC-derived Smooth Muscle Cells
⏫SMC arborization surrounding endothelial tubes
⏫SMC NOTCH3-ECD, PDGFRβ, Contractile proteins, Ca2+ activity
#StemCellRep 2026
www.cell.com/stem-cell-re...
AIM Biotech with primary or hiPSC-derived Smooth Muscle Cells
⏫SMC arborization surrounding endothelial tubes
⏫SMC NOTCH3-ECD, PDGFRβ, Contractile proteins, Ca2+ activity
#StemCellRep 2026
www.cell.com/stem-cell-re...
Boston Children’s Hospital and collaborators studied patients with multiple sclerosis and mice.
They found that a gut-driven subset of Treg cells carrying Notch3 travel to the brain, lose their calming role, and turn into inflammatory Th17 cells.
www.cell.com/immunity/ful...
They found that a gut-driven subset of Treg cells carrying Notch3 travel to the brain, lose their calming role, and turn into inflammatory Th17 cells.
www.cell.com/immunity/ful...
Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
Benamar et al. demonstrate that Notch3+ Treg cells are increased in multiple sclerosis
and in EAE. They are induced in the gut by microbiota and translocate to the CNS to
degenerate into pathogenic Th...
www.cell.com
October 2, 2025 at 4:14 PM
Boston Children’s Hospital and collaborators studied patients with multiple sclerosis and mice.
They found that a gut-driven subset of Treg cells carrying Notch3 travel to the brain, lose their calming role, and turn into inflammatory Th17 cells.
www.cell.com/immunity/ful...
They found that a gut-driven subset of Treg cells carrying Notch3 travel to the brain, lose their calming role, and turn into inflammatory Th17 cells.
www.cell.com/immunity/ful...
#SmoothMuscleCell Transcriptomic Atlas🤠
betsholtzlab.org/Publications...
Organotypicity 🫀🫁Colon Aorta
Zonation: A-V, Promixal-Distal Aorta, elastic vs muscular Artery
16 adult🐭 2780 SMC
Notch3➡️vascular SMC
Hhip➡️visceral SMC
#DevCell 2022
www.cell.com/developmenta...
betsholtzlab.org/Publications...
Organotypicity 🫀🫁Colon Aorta
Zonation: A-V, Promixal-Distal Aorta, elastic vs muscular Artery
16 adult🐭 2780 SMC
Notch3➡️vascular SMC
Hhip➡️visceral SMC
#DevCell 2022
www.cell.com/developmenta...
April 8, 2025 at 7:08 PM
#SmoothMuscleCell Transcriptomic Atlas🤠
betsholtzlab.org/Publications...
Organotypicity 🫀🫁Colon Aorta
Zonation: A-V, Promixal-Distal Aorta, elastic vs muscular Artery
16 adult🐭 2780 SMC
Notch3➡️vascular SMC
Hhip➡️visceral SMC
#DevCell 2022
www.cell.com/developmenta...
betsholtzlab.org/Publications...
Organotypicity 🫀🫁Colon Aorta
Zonation: A-V, Promixal-Distal Aorta, elastic vs muscular Artery
16 adult🐭 2780 SMC
Notch3➡️vascular SMC
Hhip➡️visceral SMC
#DevCell 2022
www.cell.com/developmenta...
Notch3+ Treg induced in the gut by microbiota translocate to CNS, outcompete protective NPY1R+-resident Treg, and degenerate into pathogenic Th17 in MS/EAE @cp-immunity.bsky.social @bostonchildrens.bsky.social
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
October 2, 2025 at 4:52 PM
Notch3+ Treg induced in the gut by microbiota translocate to CNS, outcompete protective NPY1R+-resident Treg, and degenerate into pathogenic Th17 in MS/EAE @cp-immunity.bsky.social @bostonchildrens.bsky.social
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
#Pericyte - #EndothelialCell interplay in 3D Microvessel (160-μmφ hBMMSC HUVEC) in tunable Collagen Hydrogel
Low-temp 4°C polymerized gel➡️stiff-bundled collagen fibers➡️
⏬Pericytic differentiation (⏫NOTCH3 TGFβ1)
⏫Sprouting
⏫Permeability
⏫#Pericrine IL-8
#SciAdv 2025
www.science.org/doi/full/10....
Low-temp 4°C polymerized gel➡️stiff-bundled collagen fibers➡️
⏬Pericytic differentiation (⏫NOTCH3 TGFβ1)
⏫Sprouting
⏫Permeability
⏫#Pericrine IL-8
#SciAdv 2025
www.science.org/doi/full/10....
February 4, 2025 at 1:10 PM
#Pericyte - #EndothelialCell interplay in 3D Microvessel (160-μmφ hBMMSC HUVEC) in tunable Collagen Hydrogel
Low-temp 4°C polymerized gel➡️stiff-bundled collagen fibers➡️
⏬Pericytic differentiation (⏫NOTCH3 TGFβ1)
⏫Sprouting
⏫Permeability
⏫#Pericrine IL-8
#SciAdv 2025
www.science.org/doi/full/10....
Low-temp 4°C polymerized gel➡️stiff-bundled collagen fibers➡️
⏬Pericytic differentiation (⏫NOTCH3 TGFβ1)
⏫Sprouting
⏫Permeability
⏫#Pericrine IL-8
#SciAdv 2025
www.science.org/doi/full/10....
New preprint out! Novel transgenic lines directly read out Notch3 signaling in situ, and reveal how adult neural stem cells coordinate quiescence and stemness by integrating spatiotemporal information from Delta and Jagged ligands. Read more: doi.org/10.1101/2025...
August 5, 2025 at 1:57 PM
New preprint out! Novel transgenic lines directly read out Notch3 signaling in situ, and reveal how adult neural stem cells coordinate quiescence and stemness by integrating spatiotemporal information from Delta and Jagged ligands. Read more: doi.org/10.1101/2025...
Engineer position, 12 months starting Oct. 1st, 2025, to study the role of NOTCH3 in human iPSC-derived neurogenesis models. Solid previous expertise required in cell culture, notably iPSCs, and organoid generation. Send CV, motivation letter and reference letter(s) to laure.bally-cuif@pasteur.fr
July 11, 2025 at 8:22 PM
Engineer position, 12 months starting Oct. 1st, 2025, to study the role of NOTCH3 in human iPSC-derived neurogenesis models. Solid previous expertise required in cell culture, notably iPSCs, and organoid generation. Send CV, motivation letter and reference letter(s) to laure.bally-cuif@pasteur.fr
#SpatialTranscriptomics #Visium (n=2) human #UterineLeiomyoma
+snRNAseq
Tumor vs Pseudocapsule vs Myometrium
5 ACTA2+ACTG2+ #SmoothMuscleCell states
3 PDGFRB+NOTCH3+ #Pericyte states
Love to see more analysis...🤓
ERβ⏫in angiogenic #EndothelialCell
#IntJBiolSci 2023
www.ijbs.com/v19p2515.htm
+snRNAseq
Tumor vs Pseudocapsule vs Myometrium
5 ACTA2+ACTG2+ #SmoothMuscleCell states
3 PDGFRB+NOTCH3+ #Pericyte states
Love to see more analysis...🤓
ERβ⏫in angiogenic #EndothelialCell
#IntJBiolSci 2023
www.ijbs.com/v19p2515.htm
April 20, 2025 at 6:57 PM
#SpatialTranscriptomics #Visium (n=2) human #UterineLeiomyoma
+snRNAseq
Tumor vs Pseudocapsule vs Myometrium
5 ACTA2+ACTG2+ #SmoothMuscleCell states
3 PDGFRB+NOTCH3+ #Pericyte states
Love to see more analysis...🤓
ERβ⏫in angiogenic #EndothelialCell
#IntJBiolSci 2023
www.ijbs.com/v19p2515.htm
+snRNAseq
Tumor vs Pseudocapsule vs Myometrium
5 ACTA2+ACTG2+ #SmoothMuscleCell states
3 PDGFRB+NOTCH3+ #Pericyte states
Love to see more analysis...🤓
ERβ⏫in angiogenic #EndothelialCell
#IntJBiolSci 2023
www.ijbs.com/v19p2515.htm
Antagonistic interaction between Notch3 signaling and CREB/KLF15 pathway in regulating the phenotypic alterations of glomerular parietal epithelial cells in adriamycin-induced nephropathy
#nephsky
www.sciencedirect.com/science/arti...
#nephsky
www.sciencedirect.com/science/arti...
Antagonistic interaction between Notch3 signaling and CREB/KLF15 pathway in regulating the phenotypic alterations of glomerular parietal epithelial cells in adriamycin-induced nephropathy
Parietal epithelial cells (PECs) that line the Bowman’s capsule are considered progenitor cells for podocytes, which exhibit limited regeneration capa…
www.sciencedirect.com
August 29, 2025 at 4:29 AM
Antagonistic interaction between Notch3 signaling and CREB/KLF15 pathway in regulating the phenotypic alterations of glomerular parietal epithelial cells in adriamycin-induced nephropathy
#nephsky
www.sciencedirect.com/science/arti...
#nephsky
www.sciencedirect.com/science/arti...
Multi-omic profiling reveals pericyte and smooth muscle cell contributions to CADASIL pathology in cell-specific Notch3 mutant mice
Multi-omic profiling reveals pericyte and smooth muscle cell contributions to CADASIL pathology in cell-specific Notch3 mutant mice
Huang et al. identify cell-type-specific mechanisms driving CADASIL pathology using conditional Notch3R170C mouse models. Pericyte and smooth muscle cell mutations produce distinct vascular changes, inflammation, and memory deficits. Multi-omic profiling reveals mural cell-specific pathways that contribute to the CADASIL-associated pathology.
dlvr.it
May 6, 2026 at 2:39 PM
Multi-omic profiling reveals pericyte and smooth muscle cell contributions to CADASIL pathology in cell-specific Notch3 mutant mice
Notch3+ Treg cells were induced by the gut microbiota via Toll-like receptor (TLR)-dependent mechanisms. They translocated to the central nervous system (CNS) in EAE. Notch3 interacted with delta-like ligand 1 (DLL1) on microglia to subv...
#MSchat #MS #Neurology #MultipleSclerosis #NeuroSky #MedSky
#MSchat #MS #Neurology #MultipleSclerosis #NeuroSky #MedSky
Notch3 destabilizes regulatory T cells to drive autoimmune neuroinflammation in multiple sclerosis
Notch3+ Treg cells were induced by the gut microbiota via Toll-like receptor (TLR)-dependent mechanisms. They translocated to the central nervous system (CNS) in EAE. Notch3 interacted with delta-like
gregory-ms.com
October 4, 2025 at 7:00 AM
Notch3+ Treg cells were induced by the gut microbiota via Toll-like receptor (TLR)-dependent mechanisms. They translocated to the central nervous system (CNS) in EAE. Notch3 interacted with delta-like ligand 1 (DLL1) on microglia to subv...
#MSchat #MS #Neurology #MultipleSclerosis #NeuroSky #MedSky
#MSchat #MS #Neurology #MultipleSclerosis #NeuroSky #MedSky
New from Michael Wang’s lab: a quick & easy method to detect protein structure changes — a valuable addition to tools like mass spectrometry, NMR & protein gels. Published in #asbmbJBC.
📸 Notch3 crystal structure via @rcsbpdb.bsky.social
ow.ly/x61H50WQKTN
#StructuralBIology #ASBMBJournals
📸 Notch3 crystal structure via @rcsbpdb.bsky.social
ow.ly/x61H50WQKTN
#StructuralBIology #ASBMBJournals
September 12, 2025 at 7:51 PM
New from Michael Wang’s lab: a quick & easy method to detect protein structure changes — a valuable addition to tools like mass spectrometry, NMR & protein gels. Published in #asbmbJBC.
📸 Notch3 crystal structure via @rcsbpdb.bsky.social
ow.ly/x61H50WQKTN
#StructuralBIology #ASBMBJournals
📸 Notch3 crystal structure via @rcsbpdb.bsky.social
ow.ly/x61H50WQKTN
#StructuralBIology #ASBMBJournals