#RNAdecay
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lenasteckelberg.bsky.social
Sad that #RNA2025 is almost over? Join us in August in the UK for another fun meeting on RNA! #RNAsky #RNAdecay
lenasteckelberg.bsky.social Lena Steckelberg @lenasteckelberg.bsky.social · Apr 25
Please join us for an exciting meeting on "Eukaryotic RNA turnover: from fundamentals to applications" in Brighton, UK this August! We have an exciting lineup of speakers, and will feature many selected talks. Abstract deadline is June 27! www.eventsforce.net/biochemsoc/f...
www.eventsforce.net
May 31, 2025 at 4:01 PM
milofasken.bsky.social
Great work! #RNAExosome #Assembly #RNADecay #RNA #Mammalian

Hierarchical assembly and functional resilience of the mammalian RNA exosome
Tsimafei Navalayeu...Stefan L. Ameres
www.biorxiv.org/content/10.1...
www.biorxiv.org
March 19, 2025 at 3:25 PM
jsantoyo.bsky.social
RNA methylation in retrotransposon control. #RNAmethylation #6mA #LTR #L1#ERV #RetrotransposonControl #RNAdecay #RNAstability #GenomeRegulation #CellularDifferentiation @cp-trendsgenetics.bsky.social‬
www.cell.com/trends/genet...
www.cell.com
June 7, 2025 at 10:49 AM
fariatrypslab.bsky.social
We identified 3 novel proteins at the ESB that negatively regulate genes co-transcribed with the active-VSG through specialised and spatially regulated RNA decay.
#RNA #condensates #3Dgenome #RNAdecay
May 27, 2025 at 10:28 AM
milofasken.bsky.social
Nice!
#RNAdecay #mRNAdecay #QualityControl #Cytoplasm #RNAsky #RNA

www.nature.com/articles/s41...
Cytoplasmic mRNA decay and quality control machineries in eukaryotes - Nature Reviews Genetics
In this Review, Dowdle and Lykke-Andersen discuss advances in our understanding of the machinery and mechanisms involved in the control of cytoplasmic mRNA decay and comment on implications for the de...
www.nature.com
February 6, 2025 at 6:14 PM
milofasken.bsky.social
#Dis3L2 #RNA #RNADecay
Structural and mechanistic insights into Dis3L2 mediated degradation of structured RNA
Rute G. Matos, Ankur Garg, Susana M. Costa, Patricia Pereira, Cecilia M. Arraiano, Leemor Joshua-Tor, Sandra C. Viegas
www.biorxiv.org/content/10.1...
www.biorxiv.org
August 5, 2025 at 3:17 PM
milofasken.bsky.social
A tour de force. Wonderful stuff! Congrats!
#ncRNA #MALAT1 #RNAExosome #RNaseP #NEXT #DDX59 #RNADecay #RNA #RNAsky

www.nature.com/articles/s41...
Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach - Nature Communications
Human pathways acting on nuclear ncRNAs have been refractory to forward genetics. Here, the authors develop a forward genetic approach that identifies such pathways and show DDX59 is required for mino...
www.nature.com
May 22, 2025 at 9:39 PM
milofasken.bsky.social
Cool! Congrats!

#Spt5 #Xrn2 #PolII #RNADecay #RNA #RNAsky

www.nature.com/articles/s41...
DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts - Nature Communications
The critical events in transcription termination of RNA polymerase II (Pol II) remain not fully understood. Here, the authors find that the transcription factor Spt5 enhances the activity of RNA exonu...
www.nature.com
January 5, 2025 at 9:42 PM
cmordstein.bsky.social
A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillance: Molecular Cell www.cell.com/molecular-ce...

#RNAsky #RNAbiology #RNAdecay
A nuclear RNA degradation code is recognized by PAXT for eukaryotic transcriptome surveillance
Soles et al. demonstrate that the combination of a 5′ splice site and a poly(A) junction constitutes a nuclear RNA degradation code that targets RNAs for degradation by the PAXT adaptor and the RNA ex...
www.cell.com
April 5, 2025 at 5:22 PM
genesdev.bsky.social
🆕 ADVANCE ONLINE 🆕

RESEARCH PAPER: Evolutionarily conserved spliceosome–exosome pathway in nuclear mRNA surveillance
By Abbas et al. and Elena Conti
➡️ https://ow.ly/4FuX50Z5MsN

Max Planck Institute of Biochemistry
#RNAdecay #splicing #mRNA #RNAsurveillance
June 3, 2026 at 3:00 PM
milofasken.bsky.social
#RNA #Condensate #RNAExosome #RNADecay

www.biorxiv.org/content/10.1...
RNA Dynamics Regulate Transcriptional Condensate Vivacity to Drive Gene Coordination
Transcriptional condensates (TCs), enriched with Mediator, orchestrate super-enhancer (SE)-driven gene expression critical for cell identity. However, how their dynamic physical properties shape trans...
www.biorxiv.org
June 13, 2025 at 5:49 PM
milofasken.bsky.social
Nice work from Sean Johnson on "The Molecular Mechanisms of RNA Processing in the TRAMP RNA Surveillance Complex presented today at #RNASociety #Emory GA RNA Salon! @rnasociety.bsky.social #RNA #TRAMP #RNAExosome #RNADecay #Mtr4

www.pnas.org/doi/10.1073/...
TRAMP assembly alters the conformation and RNA binding of Mtr4 and Trf4-Air2 | PNAS
The TRAMP complex contains two enzymatic activities essential for RNA processing upstream of the nuclear exosome. Within TRAMP, RNA is 3′ polyadeny...
www.pnas.org
April 18, 2025 at 6:42 PM
milofasken.bsky.social
Nice!
#RNAExosome #Structure #Review #RNADecay #RNA #RNAsky

www.annualreviews.org/content/jour...
RNA-Degrading Exosome Complexes: Molecular Mechanisms and Structural Insights | Annual Reviews
The RNA exosome is a conserved multiprotein complex essential for 3′-to-5′ RNA degradation in eukaryotic cells. In the cytoplasm, the exosome participates in messenger RNA surveillance and decay, whil...
www.annualreviews.org
June 10, 2025 at 11:17 PM
milofasken.bsky.social
#RNA #RNAdecay #XRN1 #Stress #RNAsky
Nice!
manolis-maragkakis.bsky.social Manolis Maragkakis @manolis-maragkakis.bsky.social · Dec 21
Our paper out in eLife. Full-length direct RNA #nanopore sequencing uncovers 5' #RNA shortening upon cellular #stress that depends on ✅ stress-granules and #XRN1 but ❌ not poly(A) or cap removal elifesciences.org/articles/96284

🤔 useful to further reduce translational load during #ISR?

#RNAbiology
December 21, 2024 at 7:46 AM
milofasken.bsky.social
Cool! #MTR4 #RNADecay #RNA #RNAExosome
The #nuclear #exosome co-factor #MTR4 shapes the #transcriptome for #meiotic initiation

Li Zhang, Jianshu Wang, Zhidong Tang, Zhen Lin, Ruibao Su, Naijing Hu, Yao Tang, Gaoxiang Ge, Jing Fan, Ming-Han Tong, Yuanchao Xue, Yu Zhou & Hong Cheng
rdcu.be/ed5WM
The nuclear exosome co-factor MTR4 shapes the transcriptome for meiotic initiation
Nature Communications - Nuclear RNA decay regulates gene expression in cultured cells, but its role in animals is largely unclear. Here, authors found that the nuclear exosome co-factor MTR4...
rdcu.be
March 18, 2025 at 9:08 PM
genesdev.bsky.social
RESEARCH PAPER: Evolutionarily conserved spliceosome–exosome pathway in nuclear mRNA surveillance
By Abbas et al. and Elena Conti
➡️ https://genesdev.cshlp.org/content/40/13-14/1119.full

Max Planck Institute of Biochemistry
#RNAdecay #splicing #mRNA #RNAsurveillance
July 14, 2026 at 6:00 PM
milofasken.bsky.social
Interesting! #RNADecay #NEXT #PAXT #RNAExosome #RNA
www.nature.com/articles/s41...
RNA 3′end tailing safeguards cells against products of pervasive transcription termination - Nature Communications
Pervasive transcription generates numerous unadenylated RNAs, usually degraded by the NEXT/nuclear exosome pathway. Here, the authors show that, upon NEXT inactivation, these RNAs are removed by compe...
www.nature.com
December 2, 2024 at 2:25 PM
milofasken.bsky.social
#RNADecay #RNA
www.biorxiv.org/content/10.1...
Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis
The maternal-to-zygotic transition shifts regulatory control from maternal to zygotic messenger RNAs (mRNA) through maternal mRNA degradation. While temporal aspects of maternal mRNA decay are known, ...
www.biorxiv.org
November 25, 2024 at 3:17 PM
plosbiology.org
ER-resident IRE1 plays a role in #cancer development via enzymatic activation of XPB1 TFs and regulated #RNAdecay. This study shows that some cancer cells also have an unexpected nonenzymatic dependency on IRE1 to promote #CellCycle progression & tumor growth @plosbiology.org 🧪 plos.io/3EaNFX4
Detection of endogenous Halo-tagged IRE1 and non-tagged Lamin B1 by confocal microscopy. U2OS HaloTag cells, containing a C-terminal HaloTag into the endogenous IRE1 gene locus, were examined. Cells were cultured in the presence of Janelia 646 HaloTag ligand (green), fixed, stained with anti-Lamin-B1 (red) antibodies, and analyzed by confocal microscopy. Left: Merge field of IRE1 (red), Lamin B1 (green), and nucleus (blue) is shown. Scale bar = 5 μm. Right: Close up of the nuclear envelope where each channel is shown separately and merged. Scale bar = 2 μm. Pixel size = 48 nm. Representative image of two independent experiments shown.
April 15, 2025 at 9:08 AM
milofasken.bsky.social
Interesting! #RNADecay #RNAExosome #RNA #ncRNA

Defining the roles of the #Integrator, #NEXT, and #nuclear #exosome complexes in #Drosophila #oogenesis
Yongjin Lee, Adriano Biasini, Cindy Tipping, Seong Hyeon Hong, Phillip Zamore

www.biorxiv.org/content/10.1...
Defining the roles of the Integrator, NEXT, and nuclear exosome complexes in Drosophila oogenesis
Nuclear RNA homeostasis depends on the balance of transcription, RNA processing, degradation, and transport between the nucleus and cytoplasm. RNA degradation directed by the Integrator, nuclear exoso...
www.biorxiv.org
March 13, 2025 at 3:24 PM
milofasken.bsky.social
Interesting!
#RNAExosome #EXOSC2 #RNADecay #RNAprocessing #Aging #Cancer

www.biorxiv.org/content/10.1...
Aging-dependent dysregulation of EXOSC2 is maintained in cancer as a dependency
Reprogramming of aged donor tissue cells into induced pluripotent stem cells (A-iPSC) preserved the epigenetic memory of aged-donor tissue, defined as genomic instability and poor tissue differentiati...
www.biorxiv.org
April 17, 2025 at 3:48 PM
plosbiology.org
ER-resident IRE1 plays a role in #cancer development via enzymatic activation of XPB1 TFs and regulated #RNAdecay. This study shows that some cancer cells also have an unexpected nonenzymatic dependency on IRE1 to promote #CellCycle progression & tumor growth @plosbiology.org 🧪 plos.io/3EaNFX4
Detection of endogenous Halo-tagged IRE1 and non-tagged Lamin B1 by confocal microscopy. U2OS HaloTag cells, containing a C-terminal HaloTag into the endogenous IRE1 gene locus, were examined. Cells were cultured in the presence of Janelia 646 HaloTag ligand (green), fixed, stained with anti-Lamin-B1 (red) antibodies, and analyzed by confocal microscopy. Left: Merge field of IRE1 (red), Lamin B1 (green), and nucleus (blue) is shown. Scale bar = 5 μm. Right: Close up of the nuclear envelope where each channel is shown separately and merged. Scale bar = 2 μm. Pixel size = 48 nm. Representative image of two independent experiments shown.
April 15, 2025 at 1:58 PM
plosbiology.org
ER-resident IRE1 plays a role in #cancer development via enzymatic activation of XPB1 TFs and regulated #RNAdecay. This study shows that some cancer cells also have an unexpected nonenzymatic dependency on IRE1 to promote #CellCycle progression & tumor growth @plosbiology.org 🧪 plos.io/3EaNFX4
Detection of endogenous Halo-tagged IRE1 and non-tagged Lamin B1 by confocal microscopy. U2OS HaloTag cells, containing a C-terminal HaloTag into the endogenous IRE1 gene locus, were examined. Cells were cultured in the presence of Janelia 646 HaloTag ligand (green), fixed, stained with anti-Lamin-B1 (red) antibodies, and analyzed by confocal microscopy. Left: Merge field of IRE1 (red), Lamin B1 (green), and nucleus (blue) is shown. Scale bar = 5 μm. Right: Close up of the nuclear envelope where each channel is shown separately and merged. Scale bar = 2 μm. Pixel size = 48 nm. Representative image of two independent experiments shown.
April 15, 2025 at 5:21 PM
genesdev.bsky.social
RESEARCH PAPER: Plagl1 and Lrrc58 control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503
By LaVigne et al., and Joshua Mendell
➡️ https://genesdev.cshlp.org/content/40/3-4/215.full

Josh Mendell HHMI
#TDMD #RNAdecay #miRNA #triggerRNA #development
February 9, 2026 at 7:00 PM