Grigory Tagiltsev
@grigorytagiltsev.bsky.social
Currently postdoc @briggsgroup.bsky.social @mpibiochem.bsky.social | PhD @ScheuringLab @weillcornell.bsky.social | #biophysics, #cryEM, #cryoET, #AFM
Pinned
Finally out! Working with this team was a fantastic learning experience. Also I was excited to determine the in situ clathrin structure for this project! That’s my first EM-structure upload to EMDB. Can I tell my mom I am a structural biologist now? 😁 #cryoET
Reposted by Grigory Tagiltsev
We are looking for a postdoc to investigate the principles of cellular organization using state-of-the-art microscopy! Please repost to help us find a colleague :) Full details here bio.rptu.de/fileadmin/ag...
September 3, 2026 at 2:57 PM
We are looking for a postdoc to investigate the principles of cellular organization using state-of-the-art microscopy! Please repost to help us find a colleague :) Full details here bio.rptu.de/fileadmin/ag...
Reposted by Grigory Tagiltsev
Endocytic clathrin coats are surprisingly dynamic: triskelia turn over in seconds. How is the turnover regulated and what is it for? Read Anne-Laure Boinet's preprint with @rouxlab.bsky.social www.biorxiv.org/content/10.6...
August 11, 2026 at 4:51 PM
Endocytic clathrin coats are surprisingly dynamic: triskelia turn over in seconds. How is the turnover regulated and what is it for? Read Anne-Laure Boinet's preprint with @rouxlab.bsky.social www.biorxiv.org/content/10.6...
Reposted by Grigory Tagiltsev
#AFM plus #AI is here. Say welcome to systems with autonomy level 5. TL; DR I show remote AFM operation by prompting my requests to #Claude instead of operating the system myself. This is not the future, it is happening now. youtu.be/lqVjFooYQEI?...
Claude controls an Atomic Force Microscope while the user sits at home.
YouTube video by Héctor Corte León
youtu.be
August 9, 2026 at 3:10 PM
#AFM plus #AI is here. Say welcome to systems with autonomy level 5. TL; DR I show remote AFM operation by prompting my requests to #Claude instead of operating the system myself. This is not the future, it is happening now. youtu.be/lqVjFooYQEI?...
Reposted by Grigory Tagiltsev
Take a look at our new paper on the structure of the EHD2 that, upon oligomerization, forms rings that remodel the plasma membrane and form caveolae.
Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...
Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...
August 1, 2026 at 5:56 PM
Take a look at our new paper on the structure of the EHD2 that, upon oligomerization, forms rings that remodel the plasma membrane and form caveolae.
Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...
Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...
Reposted by Grigory Tagiltsev
Structural basis of biofilm formation mediated by the Pseudomonas aeruginosa fibrillar adhesin CdrA
led by @olivia--smith.bsky.social
Collaboration with @alexbateman1.bsky.social , Andres Floto and @geiselbiofilm.bsky.social labs
led by @olivia--smith.bsky.social
Collaboration with @alexbateman1.bsky.social , Andres Floto and @geiselbiofilm.bsky.social labs
July 15, 2026 at 9:00 AM
Structural basis of biofilm formation mediated by the Pseudomonas aeruginosa fibrillar adhesin CdrA
led by @olivia--smith.bsky.social
Collaboration with @alexbateman1.bsky.social , Andres Floto and @geiselbiofilm.bsky.social labs
led by @olivia--smith.bsky.social
Collaboration with @alexbateman1.bsky.social , Andres Floto and @geiselbiofilm.bsky.social labs
Reposted by Grigory Tagiltsev
The architecture of carriers formed by AP3:ARF1 for lysosomal membrane protein transport: www.science.org/doi/10.1126/.... Work from @jggkaufman.bsky.social, in collaboration with @dgershlick.bsky.social and David Owen. @mpibiochem.bsky.social @thecimr.bsky.social
Architecture of clathrin-independent AP3:ARF1-coated carriers
AP3 generates clathrin-free carriers on endosomes by organizing via ARF1 dimers into elongated membrane-deforming arrays.
www.science.org
May 18, 2026 at 11:24 AM
The architecture of carriers formed by AP3:ARF1 for lysosomal membrane protein transport: www.science.org/doi/10.1126/.... Work from @jggkaufman.bsky.social, in collaboration with @dgershlick.bsky.social and David Owen. @mpibiochem.bsky.social @thecimr.bsky.social
Reposted by Grigory Tagiltsev
It's finally out!
Molecular architecture of mammalian AP3:ARF1 carriers - a mechanism for lysosomal membrane protein trafficking - from a combination of #biochemistry, #cryoET and #cellbiology
📝 Paper: www.science.org/doi/10.1126/...
🧵 Thread: subsequently
#teamtomo #membranetrafficking
Molecular architecture of mammalian AP3:ARF1 carriers - a mechanism for lysosomal membrane protein trafficking - from a combination of #biochemistry, #cryoET and #cellbiology
📝 Paper: www.science.org/doi/10.1126/...
🧵 Thread: subsequently
#teamtomo #membranetrafficking
The architecture of carriers formed by AP3:ARF1 for lysosomal membrane protein transport: www.science.org/doi/10.1126/.... Work from @jggkaufman.bsky.social, in collaboration with @dgershlick.bsky.social and David Owen. @mpibiochem.bsky.social @thecimr.bsky.social
Architecture of clathrin-independent AP3:ARF1-coated carriers
AP3 generates clathrin-free carriers on endosomes by organizing via ARF1 dimers into elongated membrane-deforming arrays.
www.science.org
May 18, 2026 at 8:34 PM
It's finally out!
Molecular architecture of mammalian AP3:ARF1 carriers - a mechanism for lysosomal membrane protein trafficking - from a combination of #biochemistry, #cryoET and #cellbiology
📝 Paper: www.science.org/doi/10.1126/...
🧵 Thread: subsequently
#teamtomo #membranetrafficking
Molecular architecture of mammalian AP3:ARF1 carriers - a mechanism for lysosomal membrane protein trafficking - from a combination of #biochemistry, #cryoET and #cellbiology
📝 Paper: www.science.org/doi/10.1126/...
🧵 Thread: subsequently
#teamtomo #membranetrafficking
Reposted by Grigory Tagiltsev
It's out! 🥳
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
May 14, 2026 at 11:07 AM
It's out! 🥳
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
Reposted by Grigory Tagiltsev
I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...
April 8, 2026 at 6:25 AM
I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...
Reposted by Grigory Tagiltsev
A bit late to post, but check out this peer-reviewed version at PNAS 2025 here: www.pnas.org/doi/10.1073/...
March 5, 2026 at 12:09 AM
A bit late to post, but check out this peer-reviewed version at PNAS 2025 here: www.pnas.org/doi/10.1073/...
Reposted by Grigory Tagiltsev
New paper from the lab led by @ericmulhall.bsky.social addresses how PIEZO1 and PIEZO2 are tuned to different types mechanical forces. From nanometer-scale super-resolution microscopy to in vivo experiments, links single-molecule observations to physiological function.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The molecular basis of force selectivity by PIEZO2
Nature - PIEZO2 is intrinsically more rigid than PIEZO1, and disparate mechanical stimuli paradoxically evoke opposite conformational and gating responses in each channel.
www.nature.com
March 4, 2026 at 5:19 PM
New paper from the lab led by @ericmulhall.bsky.social addresses how PIEZO1 and PIEZO2 are tuned to different types mechanical forces. From nanometer-scale super-resolution microscopy to in vivo experiments, links single-molecule observations to physiological function.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Grigory Tagiltsev
We are very excited to see this work out in its final form at Ultramicroscopy! Knife edge measurements of beam shapes and improved scanned versus shaped beam material removal comparisons. Find the OA article here: doi.org/10.1016/j.ul...
January 13, 2026 at 1:11 PM
We are very excited to see this work out in its final form at Ultramicroscopy! Knife edge measurements of beam shapes and improved scanned versus shaped beam material removal comparisons. Find the OA article here: doi.org/10.1016/j.ul...
Reposted by Grigory Tagiltsev
New preprint from the lab! 🆕
We combined in situ correlative cryo-ET 🥽🔬with multiscale simulations 🖥️⌨️ to study condensates inside yeast cells 🥼🧪
Link below 👇
We combined in situ correlative cryo-ET 🥽🔬with multiscale simulations 🖥️⌨️ to study condensates inside yeast cells 🥼🧪
Link below 👇
We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
January 13, 2026 at 9:36 PM
New preprint from the lab! 🆕
We combined in situ correlative cryo-ET 🥽🔬with multiscale simulations 🖥️⌨️ to study condensates inside yeast cells 🥼🧪
Link below 👇
We combined in situ correlative cryo-ET 🥽🔬with multiscale simulations 🖥️⌨️ to study condensates inside yeast cells 🥼🧪
Link below 👇
Reposted by Grigory Tagiltsev
Online Now: Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii Online now:
Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii
Using the latest advances in instrumentation and computational workflows, Kelley et al. present a large-scale annotated cryo-electron tomography dataset of the model green alga, Chlamydomonas reinhardtii. This unprecedented community resource is rich in high-resolution biological information and empowers the development of new methods for visual proteomics.
dlvr.it
December 24, 2025 at 8:14 PM
Online Now: Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii Online now:
Reposted by Grigory Tagiltsev
Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
December 19, 2025 at 4:45 PM
Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.
Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET
Reposted by Grigory Tagiltsev
ScienceDirect.com | Science, health and medical journals, full text articles and books.
authors.elsevier.com
December 19, 2025 at 4:13 PM
Reposted by Grigory Tagiltsev
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
October 28, 2025 at 3:07 PM
Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...
Reposted by Grigory Tagiltsev
Hey #TeamTomo …would you like to try some 🍦ICECREAM!? Our @unibas.ch colleagues over at the @ivandokmanic.bsky.social lab have developed a stunningly effective tool to de-noise and de-wedge your tomograms. The contrast and details will give you brain freeze! 🍨❄️🤯
Please try, we need feedback 🧪🧶🧬🔬
Please try, we need feedback 🧪🧶🧬🔬
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Icecream: High-Fidelity Equivariant Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...
www.biorxiv.org
October 23, 2025 at 5:57 PM
Hey #TeamTomo …would you like to try some 🍦ICECREAM!? Our @unibas.ch colleagues over at the @ivandokmanic.bsky.social lab have developed a stunningly effective tool to de-noise and de-wedge your tomograms. The contrast and details will give you brain freeze! 🍨❄️🤯
Please try, we need feedback 🧪🧶🧬🔬
Please try, we need feedback 🧪🧶🧬🔬
Reposted by Grigory Tagiltsev
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Icecream: High-Fidelity Equivariant Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...
www.biorxiv.org
October 23, 2025 at 11:13 AM
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Reposted by Grigory Tagiltsev
The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...
The mechanistic basis of cargo selection during Golgi maturation
Resident proteins of the Golgi recycle in vesicles and the protein GOLPH3 enables the COPI vesicle coat to accomplish this.
www.science.org
October 6, 2025 at 11:39 AM
The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...
Reposted by Grigory Tagiltsev
Providing order amongst constant traffic: #LMBResearch from Sean Munro (@cellbiol-mrclmb.bsky.social) with John Briggs (@mpibiochem.bsky.social) reveals how GOLPH3 allows COPI vesicles to distinguish between Golgi residents & ER-bound proteins.
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
October 6, 2025 at 8:07 AM
Providing order amongst constant traffic: #LMBResearch from Sean Munro (@cellbiol-mrclmb.bsky.social) with John Briggs (@mpibiochem.bsky.social) reveals how GOLPH3 allows COPI vesicles to distinguish between Golgi residents & ER-bound proteins.
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
Reposted by Grigory Tagiltsev
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
September 23, 2025 at 5:08 PM
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
Reposted by Grigory Tagiltsev
A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾
🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture.
buff.ly/j3TSIkn
buff.ly/j3TSIkn
September 20, 2025 at 7:24 PM
A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾
Reposted by Grigory Tagiltsev
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters - Nature Structural & Molecular Biology
Reddy et al. used ancestral protein reconstruction, cryo-electron microscopy and functional assays to elucidate how a secondary active transporter evolved to harness the energy of sodium gradients to ...
go.nature.com
September 12, 2025 at 12:08 AM
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Reposted by Grigory Tagiltsev
In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria
www.cell.com/cell-reports...
#cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social
www.cell.com/cell-reports...
#cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social
September 14, 2025 at 8:52 AM
In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria
www.cell.com/cell-reports...
#cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social
www.cell.com/cell-reports...
#cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social