#Atherosclerosis #FoamCell heterogeneity 1/2

AtheroSpectrum derives 4 transcriptomically distinct Macrophage

Foam cell Non-inflamm, Inflamm
Non-foamy Mφ Inflamm, Non-inflamm

Foam cell hi OXPHOS, Integrin, VEGF, TGFβ, IL-3

#Circ 2022 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...

#ApoA #Atherosclerosis

Apolipoprotein A-I Binding Protein deficiency augments #FoamCell formation & TLR4 dimerization on lipid raft/Inflammaraft in hyperlipidemic Ldlr-/-🐭

BODIPY-hi vs -lo lesional macrophage⬅️how much are #SmoothMuscleCell -derived?🧐

bioRxiv 2025
www.biorxiv.org/content/10.1...

#Atherosclerosis #FoamCell heterogeneity 2/2

Meta-analysis 37 scRNAseq libraries + 44 scATACseq datasets->
5 Foam cell states

ICAM1+ inflammatory
FOLR2+ phagocytic
MMP9+ matrix-remodeling
ANGPTL4+ hypoxic
COL1A2+ fibroblastic

#Biomol_MDPI 2024
www.mdpi.com/2218-273X/14...

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TREM2 as a positive regulator of Macrophage #FoamCell mitochondria, survival & efferocytosis under hypercholesterolemia

Hematopoietic TREM2 KO⏫#NecroticCore formation in early (8~12 wk) #Atherosclerosis in Ldlr-/-🐭
w/o altering lesion or Mφ burden

#NatureCVR 2024
www.nature.com/articles/s44...

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Modeling Monocyte-to-#FoamCell transition
3D #EndothelialCell (SVEC-10) #SmoothMusclecell (MOVAS) #Monocyte (RAW264.7) collagen co-culture

+Glucose/LPS

SMC⏫
Mɸ Adhesion
Mɸ Transendothelial migration
Mɸ/SMC Foamy transition

#AdvBiol 2024
onlinelibrary.wiley.com/doi/10.1002/...

#Mechanobiology #SmoothMuscleCell

#Piezo1 & Pressure (hypertensive 180/120 mmHg) ▶️cPLA2-mediated #LipidDroplet formation & #FoamCell phenotype in SMC in 2 h🤠

SMC Epigenetics by 8 h Yoda1 (Piezo1 activator): LAD-heterochromatin, H3K9me3 Cut&Tag

#AdvSci 2023
onlinelibrary.wiley.com/doi/10.1002/...

My continued biomedical education

Failure of physiological transformation & #SpiralArtery #Atherosis: their roles in #Preeclampsia

Avute Atherosis: #FoamCell lesions at poorly remodeled spiral artery

#SnoothMuscleCell or Macrophage progenies?

#AJOG_thegray 2022
www.ajog.org/article/S000...

FASN/#FattyAcidSynthase #SmoothMuscleCell - #FoamCell transition

Cholesterol->⏫FASN->⏫KLF4->
⏬ABCA1 (cholesterol efflux)
⏫SOAT1 (cholesterol esterification)

Alejandra San Martin lab #Cells_MDPI 2024
www.mdpi.com/2073-4409/13...

#Macrophage #Mitochondria #Atherosclerosis

Macrophage expressing 3β-hydroxysterol Δ24-reductase/DHCR (conv Desmosterol to Cholesterol)⏫Interferon signals, Mito ROS #inflammasome & #FoamCell formation

Myeloid DHCR KI ⏬ IL-10/-12 & LXR/RXR signals in foamy Mφ

#PNAS 2021
www.pnas.org/doi/10.1073/...

Multiplex imaging of human #Atherosclerosis
Frozen section, PhenoCycler, 9-plex imaging
DAPI, CD45, MYH11, CD68, aCasp3, IL1β, TNFα, BODIPY, CD31

Labeling #SmoothMuscleCell derived #FoamCell vs Leukocyte derived FC in🙍‍♂️coronary arteries Intima

#JAHA_AHA 2024
www.ahajournals.org/doi/10.1161/...

A nature-inspired confined cascade enzyme #Nanoreactor integrating multiple #Nanozyme activities within a #Biomimetic platform modulates inflammatory plaque microenvironments and inhibits #FoamCell formation in #Atherosclerosis.

#STTT #OpenAccess: doi.org/10.1038/s413...