#LiveCell
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giudica.bsky.social
2/5 We ran a carefully controlled benchmark on single-cell crops from LIVECell, comparing CNNs and ViTs under matched training and evaluation conditions.

Once both models were pretrained, the gap between the best CNN and best transformer was less than half a point.
September 10, 2026 at 5:40 AM
gomez-mariscal.bsky.social
#PhotoFiTT proposes a standard label-free and quantitative protocol, including #AIImageProcessing, to measure #phototoxicity derived cell stress 🧮 By systematically assessing deviations in cell behaviour, we can optimize #livecell experiments and stop blindly frying our cells💡🧫
two eggs with faces drawn on them in a frying pan with the words the egging dead below them
ALT: two eggs with faces drawn on them in a frying pan with the words the egging dead below them
media.tenor.com
December 15, 2025 at 2:58 PM
cppape.bsky.social
We compare different foundation models (SAM and DINO variants) with classical features in ilastik and fully supervised learning. The FM features outperform classical approaches. Attentive probing yields better performance than RF, but is too slow for interactive use. See example results in the fig.
Results for object segmentation based on foundation model (FM) features with a random forest, compared with supervised learning baselines and classical features. Experiments over five different datasets covering cell classification in spatial proteomics (CRC, HBM), high-content macroscopic imaging for animal phenotyping (Planri), nucleus classification in histopathology (PanNuke), and cell line classification in phase-contrast microscopy (LIVECell). The extra plot repots runtimes for model training and inference.
July 16, 2026 at 3:38 PM
cppape.bsky.social
Segmenting individual cells in microscopy is much easier these days thanks to foundation models. Can we use these models for other tasks, such as cell classification? We investigate in our latest work, finding big improvements for object and pixel classification compared to classical approaches.
Evaluation of different methods for pixel classification (top) and object classification (bottom) on the LIVECell dataset. Dark green bars show the F1-Score, which measures the classification / segmentation quality (higher is better), light green bars show the runtimes. Five different settings are compared for each task: Ilastik features + random forest (RF), microSAM embeddings + RF, SAM2 embeddings + RF, and uSAM, SAM2 + attentive probing (DeAP and ObAP). microSAM feature perform best for RF based methods, attentive probing outperforms RF based approaches, but at a much higher runtime.
July 16, 2026 at 3:38 PM
microscopy.confocalnl.com
Are you attending the 28th Annual Meeting of the Korean Society for Brain and Neural Sciences (K-Brain 2025) and the 3rd CJK Neuroscience Meeting #KSBNS2025, in Incheon, Korea?

#Neuroscience #Confocal #Imaging #Livecell #Research
August 18, 2025 at 9:13 AM
spirochrome.com
Our SPY™ probes in action for #FluorescenceFriday!

This is a live 2 cell mouse #embryo stained with SPY555-FastAct™ (grey) and SPY650-DNA (orange) going through synchronized #mitosis.

Many thanks to Blake Hernandez for providing the raw data.

#SPYprobes #LiveCell #Imaging
2 cell stage mouse embryo stained with SPY555-FastAct (grey) and SPY650-DNA (orange) going through synchronized mitosis.
November 17, 2023 at 10:35 AM
andimaging.bsky.social
New Year, New Microscope! We just got upgraded to Abberior MIRAVA POLYSCOPE — confocal → MATRIX → STED. First test looks good, large field-of-view and nice STED images using our probes.

@abberior.rocks @stefanhelllabs.bsky.social @mpi-nat.bsky.social

#microscopy #livecell #fluorescence #bioimaging
January 27, 2026 at 10:20 AM
heilemannlab.bsky.social
#FluorescenceFriday (we are watching vimentin (HaloTag7) in U2OS cells co-stained with Nile Red) #livecell #longterm
December 13, 2024 at 4:23 PM
febsletters.bsky.social
Tracking phosphoinositides is challenging—they’re dynamic & compartment-specific. This review summarizes how pairing #LiveCell imaging with advanced #MassSpectrometry enables precise, isomer-resolved quantification, offering insights into these molecules' dynamics.

➡️ doi.org/10.1002/1873-3468.70200
December 23, 2025 at 12:02 PM
juliebiteenlab.bsky.social
Thrilled to share Luis' manuscript showing how bacterial ribonucleoprotein bodies (BR-bodies) control RNA metabolism: stress changes the bacterial biomolecular condensate material state *and* shifts function from mRNA decay to storage. #LiveCell #SingleMolecule #Microscopy @schraderlab.bsky.social
Thrilled to share Luis's manuscript showing how RNA metabolism by bacterial ribonucleoprotein bodies (BR-bodies) is controlled: stress changes the bacterial biomolecular condensate material state *and* shifts function from mRNA decay to storage. #LiveCell #SingleMolecule #Microscopy Proposed model showing how the material state of BR-bodies tunes their activity, enabling a transition from mRNA decay enhancement during growth to storage during stress.
November 13, 2024 at 10:35 PM
realscientists.de
📄 Edlund, Christoffer, et al. "LIVECell-A large-scale dataset for label-free live cell segmentation."
Nature methods 18.9 (2021): 1038-1045.
January 5, 2026 at 3:18 PM
realscientists.de
Hier ein Phasenkontrastmikroskopiebild aus dem LIVECell-Datensatz. Die Zellen sind nicht eingefärbt, weshalb die Methode häufig für Echtzeitaufnahmen von lebenden Zellen verwendet wird. Die Phasenverschiebung der Lichtwellen ist abh. von Dichte+Dicke der Zelle und wird als Bildkontrast dargestellt.
Phasenkontrastmikroskopiebild, das sich durch schwachen Kontrast zwischen den eng benachbarten Zellen und dem Hintergrund auszeichnet und in Graustufen visualisiert ist
January 5, 2026 at 3:18 PM
realscientists.de
Im LIVECell-Datensatz gibt es teilweise unvollständige Labels (man nennt diese Ground Truth), was sowohl das Training als auch die Evaluation am Ende erschweren kann.
Unvollständiger Ground Truth
May 10, 2024 at 11:08 AM
realscientists.de
Wie Segmentierungsmasken für den Phasenkontrastmikroskopie-Datensatz „LIVECell“ aussehen, seht ihr in diesen Beispielbildern.
Drei beispielhafte Segmentierungsmasken aus dem LIVECell-Datensatz
May 10, 2024 at 11:04 AM
realscientists.de
📄 Edlund, Christoffer, et al. "LIVECell-A large-scale dataset for label-free live cell segmentation." Nature methods 18.9 (2021): 1038-1045.
May 6, 2024 at 2:50 PM
realscientists.de
Hier ein Phasenkontrastmikroskopiebild aus dem LIVECell-Datensatz. Die Zellen sind nicht eingefärbt, weshalb die Methode häufig für Echtzeitaufnahmen von lebenden Zellen verwendet wird. Die Phasenverschiebung der Lichtwellen ist abh. von Dichte+Dicke der Zelle und wird als Bildkontrast dargestellt.
Phasenkontrastmikrokopiebild
May 6, 2024 at 2:50 PM
cephlainc.bsky.social
Cephla is at #SfN2025 through Wednesday 11/19. Come to our booth at 3217 to check out our Squid+ spinning confocal and fluidics system + learn how we help accelerate developing/disseminating/adopting new methods and scale data production capacities!
#HCS #LiveCell #SpatialOmics #ExpansionMicroscopy
November 17, 2025 at 5:59 PM
janovictom.bsky.social
First test of live-cell single-molecule imaging in a new lab and it looks very promising!!!

Thanks to @ceitec-cellimcf.bsky.social instruments at
@ceitec.eu and @masarykuniversity.bsky.social. 🙌

#microscopy #singlemolecule #livecell #newlab
January 20, 2026 at 9:20 AM
sinaihealth.bsky.social
The Incucyte® LiveCell Analysis System at the NBCC lets researchers measure real-time cell dynamics inside the incubator. It captures continuous images and turns them into data on cell growth, movement, immune cell activity & more.

Learn more ➡️ nbcc.lunenfeld.ca/High-Content...
February 26, 2026 at 3:21 PM
microbematos.bsky.social
#OpenScience #Publication #Microscopy #CellBiology #phototoxicity #LiveCell #LiveImaging #imaging
December 16, 2025 at 11:18 AM
oni-bio.bsky.social
Are you curious about the benefits of using live cell imaging? 🔬

Our latest blog post has you covered!

Read about 7 benefits of using live-cell imaging in single-molecule research here:

zurl.co/R7Tv8 

#livecell #liveimaging #blog #SMLM #lifesciences
April 10, 2026 at 5:00 AM
oni-bio.bsky.social
Navigate your sample across scales with #AploScope: from low-power #LiveCell to high-power #SuperResolution imaging at 15 nm resolution, no need for reconfiguration. Explore biological context with multimodal imaging and easily obtain 5 color panels! 🌈 t.co/q6R1XWjec5
March 6, 2025 at 10:34 AM
okolab.bsky.social
Thanks to J. Lerner from @duke-university.bsky.social
for highlighting the importance of T/H/CO2 in-chamber feedback control ensuring top 𝗿𝗲𝗽𝗿𝗼𝗱𝘂𝗰𝗶𝗯𝗶𝗹𝗶𝘁𝘆 in #highthroughput #livecell experiments.
DM us to discover our unique #FullSampleFeedbackTechnology, how 𝗶𝘁 𝗰𝗮𝗻 𝗺𝗮𝗸𝗲 𝘆𝗼𝘂𝗿 𝗲𝘅𝗽𝗲𝗿𝗶𝗺𝗲𝗻𝘁𝘀 𝗘𝗥𝗥𝗢𝗥-𝗣𝗥𝗢𝗢𝗙
February 3, 2026 at 1:05 PM
cytosurge.bsky.social
5/
🧬 The authors acknowledge Live-seq and #FluidFM as pivotal predecessors, enabling dynamic full-transcriptome snapshots of individual cells without killing them! 😍

#SingleCell #LiveCell
May 13, 2025 at 1:59 PM
cytosurge.bsky.social
Sequence your cells while keeping them alive! How? 👇

Can't make it to Spain?😉 Connect with Tamas here: www.cytosurge.com/event/cimus-...

#LiveCell #SingleCell #SingleCellResearch #RNAseq #Biotech #Innovation #CiMUS @usc.gal @cimususc.bsky.social
cytosurge.bsky.social Cytosurge AG @cytosurge.bsky.social · Mar 7
Join us in person for this FREE seminar on Live-Cell Biopsies & #SingleCell Transfection!
March 19, 12:00–13:00, @cimusus@cimususc.bsky.social 🔗 www.cytosurge.com/event/cimus-...

Unable to join but want to elevate your project with #FluidFM? ✉️ tamas.gerecsei(at)cytosurge.com @buscusc.bsky.social
March 17, 2025 at 1:49 PM