#TwoPhoton
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cgrimm.bsky.social
We are finally online 🥳 What a fantastic adventure with @rrsims, the @emilianilab.bsky.social and all co-authors from IdV Paris #TwoPhoton #Voltagers #Holography #Rhodopsins
February 13, 2026 at 9:06 PM
doctordianap.bsky.social
Our Jove video is finally out! Watch us try our best to look natural while reading the script 🤭

#vascular #imaging #bonemarrow #leukemia #intravital #twophoton #passarolab @institutcochin.bsky.social @imag-ic.bsky.social

app.jove.com/v/67332/intr...
September 20, 2025 at 7:49 AM
kirasha.bsky.social
Imaging in a dark room when it’s sunny outside is worth it for beautiful vessels 😍 (🟢green is methoxy label of CAA). #twophoton #alzheimers #imaging
March 19, 2025 at 2:15 PM
pstemkowskiphd.bsky.social
🤔Looking to unravel your biology @bioimagingna.bsky.social I2KxBINA 2026? 💡Let's connect and explore possibilities with 🔬Femtonics' breakthrough Acousto-Optic Technology!
👉DM for a meeting or visit my poster on Wednesday, September 30th, 4:15 - 6:15 pm
#TwoPhoton #microscopy #3D #imaging #biology
September 27, 2026 at 6:08 PM
manuelacasasoli.bsky.social
"Astrocytes functionally integrate multiple synapses via specialized leaflet domains."
These domains "coordinate [...] multiple synapses and circuits active at different spatiotemporal scales, executing computations distinct from neurons".
🧪 #Science #Neuroscience #Brain
www.cell.com/cell/fulltex...
Image Source: https://www.cell.com/cell/fulltext/S0092-8674(25)01028-1 Graphical abstract In brief Volumetric high-resolution electron microscopy reveals thin astrocyte leaflets form gap-junction-interconnected domains that contain minuscule endoplasmic reticulum saccules and enwrap synapses in clusters. Twophoton calcium imaging reveals how leaflets integrate incoming neuronal inputs occurring at different spatiotemporal scales, thereby contributing to an additional layer of circuit computations.
November 13, 2025 at 5:05 PM
bozhiwu.bsky.social
Wrote a little GUI for automatic and manual alignment across multiple calcium imaging sessions. Supports either tiff or sbx formats. github.com/Bozhi-Wu/TIF...

#2P #neuroscience #calciumimaging #miniscope #imaging #twophoton #github #tiff #scanbox #neurolabware
September 16, 2025 at 1:41 AM
margolislab.bsky.social
question for 2p / laser people-

What to do with our old Ti:sapph laser (MaiTai, c. 2009) that we are replacing with a fixed wavelength fiber laser?

#twophoton #neuro #laser #craigslist?
November 13, 2024 at 3:56 AM
neuroandbeyond.bsky.social
🌟 Portable Two-Photon Microscopy for Neuroscience & Clinical Applications

Discover how Dr. Lukas Krainer turned his research at @ethzurich.bsky.social into game-changing portable two-photon imaging technology with Prospective Instruments.

youtu.be/IlZZowWkOn8

#twoPhoton #microscopy #neuroscience
January 27, 2025 at 5:25 PM
ewadorotaz.bsky.social
Co-hosted by Transcend Vivoscope & Coherent

Pizza will be served 🍕🍕🍕🍕🍕. Be on time!

#Neuroscience #Multiphoton #Mini2P #BrainImaging #InVivoImaging #HarvardMed #TwoPhoton #NeuroTech
October 27, 2025 at 1:39 AM
hearingshef.bsky.social
We've been working on a very exciting project: developing a novel approach combining surgery and #twophoton imaging🔬to investigate #calcium signalling generated spontaneously in the cochlear sensory epithelium👂in live neonatal mice 🔗: www.nature.com/articles/s41... @NatureComms
In vivo spontaneous Ca2+ activity in the pre-hearing mammalian cochlea - Nature Communications
Mechanisms underlying the initiation and modulation of the firing activity in the cochlea and its dynamics in vivo are not fully understood. Here, the authors investigate the origin and regulation of ...
www.nature.com
January 6, 2025 at 2:01 PM
achucarro.bsky.social
#AIGS26 | Day 3 (Sept 18) 🧠

Session 4: "Innovative Experimental Approaches to Studying Glial Cells.".

Speakers:
🔬 Fabio @fabiocava.bsky.social #brainoids
🔬 Minou @minoudj.bsky.social #zebrafish
🔬 Federico N. @soriafn.bsky.social #twophoton
🔬 José P. @jplopez-atalaya.bsky.social #transcriptomics
March 6, 2026 at 11:23 AM
sasspectroscopy.bsky.social
New from Applied Spectroscopy!
Quenching-Independent Two-Photon Absorption Laser-Induced #Fluorescence Measurements of Atomic Oxygen in High-Enthalpy Air/Carbon Gas–Surface Interaction
Read more: https://doi.org/10.1177/00037028251388670
#SAS #Spectroscopy #TwoPhoton #quenching #AtomicOxygen
Abstract: Understanding the abundance of atomic oxygen in the vicinity of carbon surfaces exposed to high-enthalpy flows is critical to accurate predictions of the gas–surface interaction. A novel approach for obtaining absolute number density measurements of atomic oxygen in high-enthalpy facilities with nanosecond laser pulses is described and demonstrated using photoionization-dominated, two-photon laser-induced fluorescence. In two-photon laser-induced fluorescence measurements, the depopulation of the excited state is typically dominated by electronic quenching, which depends on the temperature, pressure, and gas composition. To account for the electronic quenching rate, the fluorescence lifetime can be measured by temporally resolving the fluorescence. This can prove challenging in high-temperature and/or high-pressure environments where the fluorescence lifetime can be less than a nanosecond. Instead, by increasing the laser intensity until photoionization dominates the depopulation of the excited state, we create a quenching-independent measurement that is proportional to absolute number density. This technique is demonstrated here in the reacting boundary layer of a graphite sample ablating in the 6000 K plume of an inductively coupled plasma torch. The boundary layer possesses a large temperature gradient that varies from about 2000 K near the sample surface to the plume temperature of 6000 K in a span of approximately 2 mm. The photoionization-dominated technique is calibrated by using the freestream oxygen concentration, assuming the torch plume is in local thermodynamic equilibrium. The spatial resolution of the measurements is 50 µm and we are able to measure the number density of atomic oxygen to within about 60 µm of the graphite sample.
March 19, 2026 at 6:08 PM
scsmith4.bsky.social
Badges! Had to rearrange my work bag to fit it in, and as I'm not supposed to be openly political, I had to put the AntiFascist Noodle one on my non-work bag, but people who know will know. (And anyone who follows me here, but I'd have thought that was obvious by now.)
The back of a grey backpack, covered in badges. From the top, roughly: a large silver Space Rocks badge based on an astronaut's helmet, a #TwoPhoton Hello World badge, a cheeky grey pigeon, an NCC 1701 in a bottle, a stripy bi-with-trans-flag reading B with the T, a rainbow badge reading "I Support Diversity in Science",another Space Rocks badge, black and glittery this time, a tiny Federation logo on a starfield, a London Overground train with a poppy alongside, a stylised rocket blasting off, a small black cat, a badge for Henrietta Lacks, a pink AUTOMATIC NOODLE badge, a Cosmic Shambles logo, a NotOkayBot, a West Wing "what's next?" seal, a Vagina Museum Cliterati badge, an oil-on-water cat skeleton (to go with the other cat), a reflective badge with an orange heart shape stitched on, a Bloody Good Period logo, an Inferior badge, a SHE/HER badge, and Miffy with a bucket and spade A denim backpack flap, with two groups of badges. On the left they're sort of space-themed, but include a steogosaurus with rainbow spines, a raindow Vulcan hand, a Rocinante in a Bottle, a BB8 in bi flag colours and an R2 in Aro colours. On the right they begin with Freedom for Palestine, a Bi Progress Pride badge, a badge reading "woman's hour" above a pentacles, a Rain-stained "share you pronouns" badge, a black ANTIFACIST NOODLE badge, and a badge with a small vulva surrounded by the words "my body my choice, no exceptions"
September 5, 2025 at 1:16 PM
sasspectroscopy.bsky.social
New from Applied Spectroscopy!
Quenching-Independent Two-Photon Absorption Laser-Induced Fluorescence of Atomic Nitrogen in High-Enthalpy Air–Carbon Gas–Surface Interaction
Read more: https://doi.org/10.1177/00037028251409832
#SAS #Spectroscopy #TwoPhoton #TALIF #nitrogen #fluorescence
Abstract: A technique for quenching-independent, two-photon absorption laser-induced fluorescence (TALIF) measurements of atomic nitrogen in high-enthalpy facilities is presented. The technique relies on high-laser intensity, which results in the photoionization rate dominating other depopulation channels for the induced excited state. The photoionization-dominated technique is applied here to study the distribution of atomic nitrogen in the vicinity of an ablating graphite sample in a plasma plume. Three different plasma conditions are investigated: a 17 MJ/kg air plasma delivering 145 W/cm2 to the graphite surface, an 18 MJ/kg air plasma delivering 195 W/cm2, and a 24 MJ/kg nitrogen plasma delivering 85 W/cm2. The number density of atomic nitrogen is measured along the stagnation streamline of the flow on the graphite sample in each case. The highest number densities are observed in the nitrogen plume, as would be expected given the pure nitrogen composition and high enthalpy. The 195 W/cm2 air condition has the second-highest atomic nitrogen distribution, followed by the 145 W/cm2 air condition. This technique may provide a valuable method for studying atomic nitrogen, an important species to air/carbon gas–surface interaction at hypersonic-relevant conditions, in high-enthalpy facilities.
April 13, 2026 at 1:13 PM
bozhiwu.bsky.social
Small update on #TIFFAlign: Now supports interpolation of #2P #optogenetics blocked frames.

github.com/Bozhi-Wu/TIF...

#calciumimaging #calcium #twophoton #onephoton #miniscope
November 2, 2025 at 7:37 PM
insaninthebrain.bsky.social
fMRI, PET, MEG are used mostly on immobilized subjects, so they are not very useful to study behavior. Optic methods monitor activity with a camera and can get to a resolution of 100μm. Twophoton microscope is used to get to the deeper tissue levels but the laser pulses are very strong and short./17
March 10, 2025 at 2:17 AM
arxivlens.bsky.social
Enantiospecific two-photon electric-dipole selection rule of chiral molecules.
Li, Yong et al.
Paper
Details
#Enantiospecific #TwoPhoton #ChiralMolecules
December 5, 2025 at 9:01 AM
opensourceneuro.com
Contact us to implement Spikeling practicals at your university !
#Neurophysiology #TwoPhoton
#ComputationalNeuroscience #OpenScience #OpenHardware
February 26, 2026 at 2:50 PM